BACKGROUND:Neural stem cel transplantation provides an important way to treat sequela of traumatic brain injury, but the timing for treatment is inconclusive.
OBJECTIVE:To explore the clinical effect of neural stem cel transplantation in the treatment of sequela of traumatic brain injury and the choice of the best treatment time.
METHODS: Totaly 178 patients with sequela of traumatic brain injury who underwent neural stem cel transplantation were divided into three groups as per the timing for neural stem cel transplantation: group A (with 6 months after injury,n=60), group B (6-12 months after injury,n=59), and group C (over 12 months after injury,n=59). Improvement in clinical symptoms and scores on function independent measure (FIM) were recorded and compared in the three groups.
RESULTS AND CONCLUSION:The total effective rate of group A was significantly higher than that in groups B and C (P < 0.05). FIM scores were significantly improved in the three groups after cel transplantation (P < 0.05). At 3 months after the fourth transplantation, the FIM score in the group A was significantly higher than that in the other two groups, and the incidence of adverse reactions in the group A was significantly lower than that in the other two groups (P < 0.05). These findings indicate that neural stem cel transplantation at different timing can al harvest certain clinical effects, but the best timing for neural stem cel transplantation is within 6 months after injury.

Objective To investigate the diagnostic values of vaginitis five of the joint inspection kit combined with microscopic examination for common vaginal disease.Methods The vaginal secretions samples from 4 114 outpatients were tested with LTS-V400 vaginitis five of the joint inspection kit and microscopic examination.The examination results of age groups were analyzed and compared.Results In all 4 114 cases of samples,the positive rate of trichomonad(1.95%)was significantly lower than that of fun-gi(4.74%),P <0.05.The positive rates of N-acetyl-beta-galactosamine glycosides enzyme(NAG),sialidase(SNA),and leukocyte esterase(LE)were the highest in >40-50-age group,which were 1.95%,6.10%,14.15%,10.24% and 46.34%,respectively. The positive rates of H2 O2 ,pH >4.5 and pH < 3.8 were the highest in > 50 age group,which were 85.43%,86.09%,and 0.66%,respectively.The positive rates of trichomonad,SNA,LE,H2 O2 ,pH>4.5 and pH<3.8 were statistically different a-mong the age groups(P <0.05 ).Conclusion Vaginitis five of the joint inspection kit combined with microscopic examination is suitable for the diagnosis of trichomonas vaginitis,mouldsex vaginitis,and bacterial vaginal disease .

AIM:To evaluate the antitumor effect of caffeic acid Ge on U14 tumor bearing mice.METHODS:The tumor inhibitory ratios of caffeic acid Ge on the growth of U14 in mice was observed.Apoptosis morphological transformation of U14 cells induced by caffeic acid Ge was detected by electronic scan microscope and MG-P staining.Alteration of cell cycle was analyzed by flow cytometry.Apoptosis-related protein levels of Bax and Bcl-2 were determined by immunity histochemistry technology.MTT assay was applied to study the antitumor activities of caffeic acid Ge in U14 cell lines in vitro.RESULTS:Tumor inhibitory rates in caffeic-acid Ge groups were 38.50%,47.17% and 64.02%(from low dose to high dose)(P

Objective:To explore the effect of online group positive psychological intervention on the professional quality of life and professional values of oncology nurses.Methods:From November 2020 to March 2021, 100 oncology nurses from Peking University Cancer Hospital were selected as the study subjects. The nurses were divided into intervention group (50 cases) and control group (50 cases) by random number table. The control group received routine intervention, and the intervention group received online group intervention with the theme of "happy work" on the basis of the control group. The professional quality of life and professional values of the two groups of nurses were compared before, immediately after, one month and three months after the intervention.Results:Immediately after intervention, the score of compassion satisfaction in the intervention group was higher than that in the control group and before intervention, and the score of burnout was lower than that in the control group and before intervention. One month after the intervention, the score of compassion satisfaction in the intervention group was higher than that in the control group and before intervention. Three months after the intervention, the score of professional values in the intervention group was higher than that before intervention. The above differences were statistically significant ( P<0.05) . Conclusions:Online group positive psychological intervention can improve the professional quality of life of oncology nurses in a short time, and has an effect on promoting professional values.

Objective:To retrieve and analyze the status quo and hotspots of death anxiety research, and provide directions for further research in death anxiety.Methods:Articles concerning death anxiety in their titles, keywords and abstracts published up to July 10, 2020 in core journals from CNKI were systematically retrieved. The retrieved results were analyzed bibliometrically using the bibliographic information co-occurrence analysis system (Bicomb) , including year of publication, source journal, keywords, and authors, etc. The high-frequency words were extracted by calculation and customization, and a word-part matrix was derived. The word-part matrix was clustered with GCLUTO 1.0 software, and the current status and research hotspots were derived from the specific articles.Results:A total of 79 death anxiety-related papers were published, with 19 key words appearing ≥ 3 times. Four research hotspots of death anxiety were obtained through analysis: death anxiety of nursing staff, death anxiety of terminal patients, development and adaptation of death anxiety scale, and death anxiety and mental health.Conclusions:Death anxiety research has entered the developmental stage; studies are mostly conducted among the aged and caregivers with easily accessible samples; the types of studies are mainly empirical studies and cross-sectional surveys. In the future, we may further break through the limitation of sample accessibility, strengthen cooperation and communication between authors and institutions, and try to conduct multi-center, large-sample, effective intervention studies to alleviate death anxiety and improve the quality of caregiving.

Objective:To explore the mediating effect of professional values on the stress overload and professional quality of life in oncology nurses.Methods:Using the convenient sampling method, a total of 544 oncology nurses from 2 cancer hospitals in Beijing were selected as the research objects. General data questionnaire, Stress Overload Scale (SOS) , The Nurses Professional Values Scale-Revised (NPVSR) , revised version of Nurses Professional Values Scale and Chinese version of Professional Quality of Life Scale were used for questionnaire survey and correlation analysis of the data. AMOS 21.0 software was used to establish a structural equation model to verify the mediating effect.Results:The stress overload score of oncology nurses was 48.00 (36.00, 62.00) , and the professional values score was 102.00 (86.25, 110.00) . The compassion satisfaction score for professional quality of life was 36.00 (31.00, 41.00) , the burnout score was 22.00 (17.00, 26.00) , and the secondary traumatic stress score was 22.00 (19.00, 26.00) . The stress overload of oncology nurses was negatively correlated with professional values, negatively correlated with compassion satisfaction, and positively correlated with job burnout and secondary traumatic stress ( P<0.05) . Structural equation fitting index ( CMIF/ DF) was 0.430, goodness of fit index ( GFI) was 1.000, adjusted goodness of fit index ( AGFI) was 0.995 and root mean square error of approximation ( RMSEA) was 0.000. The stress overload and professional values of oncology nurses had a direct predictive effect on their professional quality of life. The stress overload was mediated by professional values, which indirectly affected compassion satisfaction and job burnout. Conclusions:Nursing managers can enhance their professional values and improve their professional quality of life by reducing the stress overload on oncology nurses.

Objective:To understand the attitude and knowledge on cancer pain management among oncology department nurses and to explore their influencing factors so as to provide a basis for developing targeted pain training projects.Methods:From April to June 2018, we investigated 291 oncology department nurses from 21 provinces, 4 municipalities directly under the central government and 4 autonomous regions on the spot with the self-designed information questionnaire and the Knowledge and Attitudes Survey Regarding Pain (KASRP) by convenience sampling.Results:A total of 276 valid questionnaires were collected. Among 276 nurses, the accuracy rate of KASRP was (59.2±11.0) % ranged from 31.7% to 85.4%. There were statistical differences in the accuracy rates of KASRP among nurses with different areas, years working in cancer nursing, whether attended the pain training, whether participated in cancer pain management practice and monthly number of patients with cancer pain nursing provided by nurses ( P<0.05) . Correlation analysis showed that the age of nurses had a positive correlation with the score of KASRP ( r=0.237, P<0.001) . Multiple linear regression analysis proved that the influencing factors of attitude and knowledge on cancer pain management of nurses included the age of nurses, attending pain management practice, participating in the pain training ( P<0.05) . Conclusions:Among oncology department nurses in China, there are misunderstandings in attitudes on cancer pain management, and their related knowledge was insufficient which influenced by many factors. On the one hand, it points out the importance and urgency for clinical nurses to develop targeted pain management pain. On the other hand, we should provide innovative training methods and combine pain management practice tightly to effectively improve attitude and knowledge on cancer pain management among of nurses so as to make nurses play an important role well in cancer pain management.

Pathological cardiac hypertrophy serves as a significant foundation for cardiac dysfunction and heart failure. Recently, growing evidence has revealed that microRNAs (miRNAs) play multiple roles in biological processes and participate in cardiovascular diseases. In the present research, we investigate the impact of miRNA-34c-5p on cardiac hypertrophy and the mechanism involved. The expression of miR-34c-5p was proved to be elevated in heart tissues from isoprenaline (ISO)-infused mice. ISO also promoted miR-34c-5p level in primary cultures of neonatal rat cardiomyocytes (NRCMs). Transfection with miR-34c-5p mimic enhanced cell surface area and expression levels of foetal-type genes atrial natriuretic factor (Anf) and β-myosin heavy chain (β-Mhc) in NRCMs. In contrast, treatment with miR-34c-5p inhibitor attenuated ISO-induced hypertrophic responses. Enforced expression of miR-34c-5p by tail intravenous injection of its agomir led to cardiac dysfunction and hypertrophy in mice, whereas inhibiting miR-34c-5p by specific antagomir could protect the animals against ISO-triggered hypertrophic abnormalities. Mechanistically, miR-34c-5p suppressed autophagic flux in cardiomyocytes, which contributed to the development of hypertrophy. Furthermore, the autophagy-related gene 4B (ATG4B) was identified as a direct target of miR-34c-5p, and miR-34c-5p was certified to interact with 3' untranslated region of Atg4b mRNA by dual-luciferase reporter assay. miR-34c-5p reduced the expression of ATG4B, thereby resulting in decreased autophagy activity and induction of hypertrophy. Inhibition of miR-34c-5p abolished the detrimental effects of ISO by restoring ATG4B and increasing autophagy. In conclusion, our findings illuminate that miR-34c-5p participates in ISO-induced cardiac hypertrophy, at least partly through suppressing ATG4B and autophagy. It suggests that regulation of miR-34c-5p may offer a new way for handling hypertrophy-related cardiac dysfunction.