Objective To study the biological effects of dose radiotherapy for primary liver cancer patients with stereotactic body portion.Methods 80 patients with primary liver cancer were selected.According to a random number table method, all patients were divided into observation group and control group,40 patients in each group.All patients were treated with stereotactic body radiotherapy treatment, the observation group was treated with high dose, while the control group received low-dose.The radiotherapy effects of different doses were analyzed and compared.Results After treatment, the effective rate of the observation group was 77.5%,which of the control group was 55.0%, ,the difference between the two groups was statistically significant (x2=4.528,P=0.033).After treatment for 6 months,1 year and 2 years, the survival rates of the observation group were 77.5%,75.0%,72.5%, which were significantly higher than 55.5%,52.5%,50.0% of the control group (P<0.05).The incidence rate of adverse reactions of the observation group was 42.5%, which was significantly lower than 72.5% of the control group (x2=7.365,P=0.006).Conclusion Stereotactic body radiotherapy treatment with different therapeutic dose has different treatment efficacy, high-dose SBRT treatment has significantly higher efficacy, survival rate and lower adverse reactions, which is worthy of clinical application.

Objective To study the biological effect of different dose of satereotactic body radiation therapy (SBRT) on hepatoma Hep G2 Cells.Methods Hep G2 hepatumor cells vitality of 0,2,4,6,8,and 10 Gy doses of SBRT were determined by methyl thiazolyl tetrazolium (MTT) method.The cell colony formation rate and the cell survival fraction were detected.The cell apoptosis was detected by flow cytometry.The expressions of cyclin D1,Bax,Bcl-2,and caspase-3 protein were detected by Western blot.Results Hep G2 cells vitality was obvious inhibitory effect in different doses of SBRT,increasing with the SBRT dose,the cell vitality inhibition [2 Gy group (4.29 ± 0.63) ％,4 Gy group (19.37 ± 4.54) ％,6 Gy group (41.35 ±4.51)％,8 Gy group (86.34 ±5.68)％,10 Gy group (97.24 ±5.41)％] was increased,compared to 0 Gy group [(2.94 ± 0.52) ％],the difference was statistically significant (all P ＜ 0.01 or P ＜0.05).The cell colony formation rate and survival score in different doses of SBRT were statistically lower than the control group,increasing with the SBRT dose,the cell colony formation rate [4 Gy group (83.15 ± 4.71),6 Gy group (65.23 ± 5.47),8 Gy group (41.28 ± 5.36),10 Gy group (8.14 ± 5.22)] and survival score [2 Gy group (95.49 ±3.21),4 Gy group (84.23 ±3.45),6 Gy group (66.08 ± 3.71),8 Gy group (41.82 ± 3.82),10 Gy group (8.25 ± 3.56)] were decreased,compared to 0 Gy group [(98.72 ± 1.23),(100.00 ±0.00)],the differences were statistically significant (all P ＜0.01 or P ＜ 0.05).The cell apoptosis in different doses of SBRT was increased,increasing with the SBRT dose,the Hep G2 cells apoptosis [4 Gy group (13.67 ±4.62),6 Gy group (28.71 ±5.13),8 Gy group (54.16 ±5.24),10 Gy group (76.31 ± 5.17)] were significantly increased,compared to 0 Gy group (1.35 ±0.46),the difference was statistically significant (all P ＜ 0.01).The expressions of cyclinD1,Bax,Bcl-2,and caspase-3 protein in different doses of SBRT were decreased,increasing with the SBRT dose,the cyclin D1 [6 Gy group (1.94 ± 0.57),8 Gy group (1.38 ± 0.41),10 Gy group (0.52 ± 0.34)],Bax[6 Gy group (2.67 ±0.59),8 Gy group (1.73 ±0.45),10 Gy group (0.63 ±0.37)],Bcl-2 [4 Gy group (1.78 ±0.41),6 Gy group (1.03 ±0.35),8 Gy group (0.76 ±0.28),10 Gy group (0.38 ± 0.21)],Caspase-3 [6 Gy group (0.76 ± 0.31),8 Gy group (0.42 ± 0.24),and 10 Gy group (0.24 ± 0.21)] were obviously decreased,compared to 0 Gy group [(3.41 ± 0.75),(4.36 ± 0.87),(2.57 ± 0.46),(1.67 ± 0.55)],the difference was statistically significant (all P ＜ 0.05).Conclusions Different dose of SBRT can reduce Hep G2 cell vitality,enhance radiation sensitivity,have a dose dependent,maybe related to promote cell apoptosis and inhibit the proliferation of protein expression.

Objective To analyze the differential value of gastrointestinal stromal tumors and non-gastrointestinal stromal tumor by cytokeratin-19 fragment(CYFRA21-1),protein kinase B(PKB)and carbohydrate antigen 19-9(CA19-9)combined with endoscopic ultrasonography(EUS).Methods 69 patients with gastrointestinal stromal tumors from January 2020 to July 2023 were selected as the study group,and 78 patients with non-gastrointestinal stromal tumor(they were 25 cases of gastrointestinal leiomyoma and 53 cases of gastrointestinal schwannoma)were selected as the control group during the same period prospectively.The EUS indicators and tumor markers in the two groups were compared.Receiver operator characteristic curve(ROC curve)was drawn to analyze the diagnostic value of serum levels of CYFRA21-1,PKB and CA19-9 alone and in combination for gastrointestinal stromal tumors.Results Compared with the control group,the mean gray level,gray standard deviation and serum levels of CYFRA21-1,PKB and CA19-9 in the study group were higher(P<0.05).There was no significant difference in mean gray level,gray standard deviation and serum levels of CYFRA21-1,PKB and CA19-9 in patients with gastrointestinal stromal tumors of different genders and ages(P>0.05).Compared with patients with gastrointestinal stromal tumors with tumor diameter≤5 cm and pathological mitotic figure≤5/50 HPF,the mean gray level,gray standard deviation and serum levels of CYFRA21-1,PKB and CA19-9 were higher in patients with gastrointestinal stromal tumors with tumor diameter>5 cm and pathological mitotic figure>5/50 HPF(P<0.05).Gastrointestinal stromal tumors were included as positive and non-gastrointestinal stromal tumor as negative.ROC curve was drawn to analyze the diagnostic value of gray mean value,gray standard deviation,serum levels of CYFRA21-1,PKB,and CA19-9 alone and in combination for gastrointestinal stromal tumors,and the combined detection was the highest,area under the curve(AUC)was 0.936,the sensitivity was 82.61%,and the specificity was 91.03%.Conclusion With the occurrence and development of gastrointestinal stromal tumors,CYFRA21-1,PKB,CA19-9 levels,gray mean value and gray standard deviation increase,and levels of CYFRA21-1,PKB,CA19-9 combined with EUS are valuable in the diagnosis of gastrointestinal stromal tumors.