Our previous study showed that malignant phenotype of human promyelocytic leukemia cells could he suppressed by fusion of them with mouse reticulocytes. In order to investigate the morphological changes for malignancy reversion, the present experiment was designed to study the microscepic and submicroscopic structure of cybrid cells and compared with their parental tumor cells. The results indicated that during the short period of cybrid cell cultivation, nuclei of numerous cybrid cells became pyknotic and eccentric, and some cells showed the process of nuclear expulsion (denucleation). The cybrids which cultivated for long period in vitro developed into more mature cells along both myeloid and erythroid differentiation pathway. The effects of mouse reticulocyte cytoplasmic factor on differentiation pathway of human promyelocytic leukemia cells were discussed.

The heterospecies hybrid cells(HL-N)from the fusion of human promyelocy-tic leukemia mutant cells(HL-60-AR)and mouse bone marrow nucleated red cellswere established in HAT selective medium.Malignant phenotype comparative analy-sis between parental tumor cells and hybrid cells showed that growth ability ofhybrid cells was decreased.The hybrid cells reduced their DNA synthesis rate andlost the ability of colony-forming in 0.3% soft agar medium.The cells lost tumor-producing ability when they were transplanted into nude mice also.Inhibition orreduction of c-myc oncogene expression was demonstrated by Northern molecularhybridization techniques.The ultrastructure of hybrid cells were also different fromtheir parental cells.These results mentioned above showed that the mouse bone mar-row nucleated red cells might provide some peculiar factors(both nuclear factorsand cytoplasmic factors)to inhibit the expression of HL-60-AR cell malignant phe-notypes.

Retrospective analysis of 761 case-times successive color Doppler sonography for 518 renal transplant patients was carried out.six parameters of sonography were used to compare, the CsA-NT hemodynamic changes among the patients with normal function, rejected and hy-dronephrotic kidney.It revealed that during rejection of the rendl srafts, besides an elevation of resistance index(RI)and a decrease of D/S ratio, the velocity and volume of renal blood flow were also slowed down and decreased respectively.After combating rejection, the renal function recovered to normal gradually, and RI dropped below 0.75 subsequently.Otherwise R1 would be persistently elevated, which indicated an irreversible rejection.The diagnosis of acute rejection was given at RI≥0.83, with the sensitivity rate being 82.70%and specificity being 80.10%.It was suggested that RI is a relatively sensitive and relialble parameter for the diagnosis of rejection.In combination with the clinical manifestations RI is of great important clinical value for the early diagnosis and treatment of acute rejection.

BACKGROUND:Biodegradable implants cannot only rebuild bone defect site, moreover, with the gradual degradation of the materials, new bone tissue can completely replace the graft materials to fil bone defects. OBJECTIVE:To summarize the research progress of biodegradable materials combined with osteogenic factor in orthopedics. METHODS: We took the “biodegradable materials, factors, cel active factor, bone tissue engineering” as the search terms in Chinese and English, respectively, to retrieve the related literatures from PubMed, Wanfang and CNKI database during 2000 to 2015 by computer. RESULTS AND CONCLUSION: Biodegradable medical polymer materials can be divided into natural polymer materials and synthetic biodegradable materials. Natural polymeric materials have good biocompatibility, but poor mechanical strength. The mechanical strength of synthetic biodegradable materials is higher than that of natural polymer materials, but the synthetic biodegradable materials are likely to cause local accumulation of acidic substances, produce local inflammation. The biodegradable medical polymer materials combined with osteogenic factor can improve the mechanical strength and osteoinductive ability of materials, but as a bone repair material, it stil has many problems to be solved.

ObjectiveTo explore the inflammatory factors C-reactive protein (CRP),interleukin (IL)-6,tumor necrosis factor (TNF)-α and white blood cell (WBC) count differences between acute myocardial infarction(AMI ) thrombolysis treatment and unthormbolysis treatment,and find out the relevance between the inflammatory factors and the prognosis.MethodsAccording to the condition of accepting AMI thrombolysis treatment,the 229 patients of AMI were divided into the thrombolysis group( 131 cases) and the unthrombolysis group(98 cases).The levels of myocardial troponin I (cTnI),creatine kinase(CK),creatine kinase isoenzyme-MB (CK-MB) were detected at the time of patients sent into the hospital for the immediate,6-hour later and 24-hour later.After 6-month's follow-up,prognosis was compared between two groups.ResultsTwenty-seven cases lost in the thrombolysis group.One case died within 6 months and the mortality was 1.0％(1/104) in the thrombolysis group,and 6 cases died within 6 months and the mortality was 6.1％(6/98 ) in the unthrombolysis group.There was significant difference between two groups (P ＜ 0.05 ).The levels of CK,CK-MB in the thrombolysis group advanced,and compared with that in the unthrombolysis group,there were significant differences (P ＜ 0.05 ).The levels of TNF-α,IL-6 in the thrombolysis group were significantly higher than those in the unthrombolysis group (P＜ 0.05),and CRP and WBC count had no significant differences between two groups (P ＞ 0.05).The repatency rate was 79.4％( 104/131 ) in the thrombolysis group,the levels of TNF- α,IL-6 in repatency patients were higher than those in non-repatency patients.There were significant differences(P ＜ 0.05 ).ConclusionsThe thrombolysis is an effective way to cure AMI.The increase of TNF- α,IL-6 after the thrombolysis is considered to be related to reperfusion injury,and CRP,IL-6,TNF-α and WBC count can forecast the inflammation of myocardial necrosis and take an impotant role in predicting the prognosis of the AMI.The antiinflammatory and antioxidation treatment is significant to improve the prognosis of the AMI.

[Objective] To evalvate the influence of total knee replacement(TKR)on the lipid peroxdation in erythrocytes,and the prophylactic treatment of Vitamin E and fructose 1,6-diphosphate(FDP)on it.[Method]Totally 60 patients of knee osteoarthritis were divided into control group,Vitamin E group,Fructose 1,6-diphosphate(FDP)group and Vitamin E added FDP group.Blood samples were taken for biochemical determination of MDA and Cu-Zn-SOD before and after the operation at 1,3,5 and 7 days.[Result]MDA level in erythrocytes increased singnificantly after TKR compared with that before operation(P

Objective: To investigate effective matching between computerized physician order entry and charging items in the hospital. Methods: Standard data of computerized physician order has been established, in which physician order items and charging items have been matched. It has effectively solved the problems in the process of computerized physician order entry. Results and Conclusion: The implementation of matching computerized physician and costs has effectively controlled the errors and missing of costs, which released the complaint of patients and received the good reputation and welcome, the compliance of doctors using computerized physician order entry has been obviously improved, meanwhile, it has provided new application experiences for implementing the following charging of national medical services prices in hospitals.

The present study is designed to establish a xenograft model of human promyelocytic leukemia cell mutant (HL-60-AR) deficient in hypoxanthine guanine phosphoribosyltransferase (HGPRT) in nude mice. A solid leukemia sarcoma developed after subcutaneous inoculation with HL-60-AR cells. Comparative studies of HL-60-AR/Nu tumor cells in nude mice and cultured HL-60-AR cells in vitro revealed virtual identity as shown by light microscopic morphology, ultrastructure of cell, cytochemistry, chromosome analysis, LDH isoenzyme pattern, genetic markers and differentiated characters assay. Up to now, twelve generations haw been transmitted in rive by inoculating with the solid tumor Cells developed in nude mice. This nude mice model in which human leukemia cells grew could be considered as a useful model for in rive studies of human leukemic cells proliferation, differentiation and the screening for anti-leukemia drugs.

Objective To study the classification, management and outcome of the fracture of ulna coronoid process. Methods Retrospective analysis was carried out in 31 patients with fracture of ulna coronoid process. There were 19 males and 12 females with an average age of 29.8 years old (range, 18-52 years old). The fractures of ulna coronoid process were classified into 4 major groups based on the condition of the injury of ulna coronoid process, the anterior bundle of the ulnar collateral ligaments(UCL) and the elbow stability. The fracture of coronoid process within half of the height was defined as type Ⅰ. The half of coronoid process fracture with the injury of UCL was defined as type Ⅱ. The base of coronoid process fracture with the dislocation of elbow joint was defined as type Ⅲ, sometimes with the injury of UCL. The severe comminute fracture of coronoid process with elbow instability was defined as type Ⅳ. There were 11 cases in typeⅠ, 9 typeⅡ, 6 type Ⅲ and 5 type Ⅳ in our group. According to the type of injury we choose correlative treatment. Results The follow-up was 18-72 months(average 28.6 months). All the patients got fracture union, and without inflammation, neural injuries and elbow instability. 1 type Ⅲ and 2 type Ⅳ had traumatic osteoarthritis, and 2 type Ⅲ and 2 type Ⅳ occurred heterotopic ossification. There was a statistical significance when the motion range of two-side joints was compared in type Ⅳ. Conclusion We choose conservative treatment in the fracture of typeⅠ. But if the piece of bone affected motion of elbow joint, we can choose operative treatment, and elbow stability is not affected. The fracture of type Ⅱ and type Ⅲ with elbow instability should be reduced internal fixation and the ligament must be repaired or reconstructed. In cases of type Ⅳ, bone reconstruction is necessary to recover of elbow stability. Proper post-operative rehabilitation can decrease the rate of traumatic osteoarthritis.