Objective To establish the determination of prim-O-glucosylcimifugin in Biyan Tablets by HPLC.Methods The HPLC determination was carried out on Diamonsil C18 column(4.6 mm? 250 mm,5 ? m).The mobile phase consisted of methanol-acetonirile-H2O(18 :12 :70) with a flow rate of 1.0 mL/min,and the ultraviolet detection wavelength was set at 254 nm.Results Prim-O-glucosylcimifugin showed a good linearity in the range of 0.1888～ 1.534 ? g with the peak area score,the coefficient was 0.9997,and the average recovery was 101.74 %(RSD=2.97 %).Conclusion The method is accurate and reliable,and can be used for the quality control of Biyan Tablets.

[Objective] To establish a method for rapid identification of Fructus Citri Sarcodactylis (FCS) from different production areas. [Methods] Fourier transform infrared (FTIR) spectroscopy was adopted to identify FCS. The height and position of the characteristic absorption peaks, and I value (a quantitative parameter of peak height ratio) were compared in FCS from different production areas. [Results] Each kind of FCS had their fixed range in peak height ratio I: 0.7-0.9 in FCS from Guangdong, 1.3-1.6 in FCS from Sichuan, 0.9-1.1 in FCS from Zhejiang, indicating that I value can be used to identify FCS from different areas . [Conclusion] For the identification of FCS from different production areas, FTIR spectroscopy is an effective method, in which there is no need for isolation, purification and chemical treatment.

In continuation of our investigation, a new diterpene, rabdonervosin C (Ⅰ), was isolat-ed from the leaves and stems of Rabdosia nervosa (Hemsl.) C. Y. Wu et H. W. Li. Its structure wasidentified as lα, 15β, -dihydroxy-6β-ethoxy-6, 7-B-seco-ent-kaur-16-en-6, 20-epoxy-7, 20-δ-olide on thebasis of spectroscopic (MS, IR, 1HNMR, 13CNMR, DEPT and 2D-NMR) data and in comparison with theknown rabdonervosin A(Ⅲ) and B (Ⅱ).

篈 new diterpene,rabdonervosin A(I ) was isolated from an ethanolic extract of the leaves and stems ofRabdosia nervosa co1lected in Jiangxi,China. Its structure was established as 1Q, 69, 15Attihydroxy-6, 7-B-sec-oent-kaur-16-en-6, 20-epoxy-7, 20-8-olide on basis of spectroscopic data.

Objective To develop a HPLC method for the determination of Lasiokaurin in the stems and leaves of Rabdosia nervosa.Methods HPLC was performed on a Kromsil C18 reversed-phase column(250mm?4.2mm,5?m).All of the reference substances and samples were separated with the mobile phase of methanol :water(55:45) under isocratic elution for 30min,flow rate was 1.0ml/min,the detection wavelength was 234nm,and the column temperature was 30℃.Results The content of Lasiokaurin was 0.005mg/g in the stems and 0.372mg/g in the leaves,the content in the stems were almost 1/75 times as much as that in the leaves.The average recovery of Lasiokaurin was 96.21%.Conclusion This developed method,which is simple and with good precision,high sensitivity and selectivity,can be used for quality evaluation of Lasiokaurin in Rabdosia nervosa.

Objective To determine the icariin content in Qianlie Shule Granules (QSG). Methods After proper pre-treatment,HPLC method was performed on a C18 column. The mobile phase consisted of acetonitrile-water (29 ∶71) and the UV d etective wavelength was set at 270 nm. Results The peak of icariin was separated completely. The calibration curve of icariin was linear within 0.16～1.2 mg?mL -1,Y=0.028 7X-0.009 62 (r=0.999 9). Conclusion This method is accurate and su itable for the determination of icariin content.

AIM:To study the chemical constituents of Fissistigma oldhamii(Hemsl.)Merr.METHODS:Silica gel column chromatography was used in the isolation procedure,the structures of the isolated compounds were elucidated by spectral data.Meanwhile cytotoxic activity of compound Ⅰ was made according to the cell experiment in vitro.RESULTS:Five compounds were isolated from Radix of Fissistigma oldhamii(Hemsl.)Merr..On the basis of physical-chemical constants and spectral data(EI-MS,1H-NMR,13C-NMR),these compounds were identified as:Aristolactam A Ⅱ(Ⅰ),Aristolactam B(Ⅱ),Physcion(Ⅲ),?-sitosterol(Ⅳ),Stigmastan-7-one(Ⅴ).Compound Ⅰ showed cytotoxic activities on GLC-82 and HL_ 60 cell strains.CONCLUSION:Compounds Ⅲ,Ⅳ and Ⅴ are firstly isolated from Fissistigma oldhamii(Hemsl.)Merr..The IC_ 50 of compound Ⅰ on GLC-82 and HL_ 60 cell strain are:234.45,101.17 ?M.

Objective To study the chemical constituents of Evodia lepta.Methods The chemical constituents were isolated by chromatographic methods and their structures were elucidated by physicochemical characteristics and spectral data.Results Two compounds were isolated and their structures were identified as erythro-3-(1',2',3'-trihydroxy) isopentyl-7-hydroxycoumarin(Ⅰ) and?-daucosterol (Ⅱ).Conclusion CompoundⅠis a new one named evodosin A while compoundⅡis isolated from E. lepta for the first time.

Objective To develop a HPLC method for the determination of oridonin in the leaves and stems of Rabdosia nervosa.Methods All of reference substances and samples were separated with the mobile phase of methanol:water (45 ∶55) under isocratic elution for 20 min on a Kromasil C18 reversed-phase column(250 mm?4.0 mm,5 ?m),the flow rate was 1.0 mL?min-1,the detection wavelength was 235 nm,and the column temperature was 30 ℃.Results The content of oridonin was 0.047 mg/g in stems and 0.791 mg/g in leaves,the content in stems being about one seventeenth of that in leaves.The average recovery of oridonin was 98.33 %.Conclusion This method is simple,sensitive,accurate and suitable for quantitative determination of oridonin in Rabdosia nervosa.

Objective To study the constituents in Melicope pteleifolia. Methods Plant material was isolated with 80% EtOH. Compounds were separated with chromatographic methods and their structures were elucidated on the basis of spectral analysis (EI-MS, 1H-NMR, and 13C-NMR) and chemical evidence. Results Five compounds were isolated from petrol ether or ethyl acetate soluble fraction. Their structures were identified as 3,5,3'-trihydroxy-4'-methoxy-7-(3-methylbut-2-enyloxy) flavone (pteleifolosin C, 1), 3,7-dimethoxyl kaempferol (kamatakenin, 2), vanillic acid (3), tricosanoic acid tetradecyl ester (4), and p-sitosterol (5), respectively. Conclusion Compound 1 is a new structure named pteleifolosin C. Compounds 2-4 are isolated from this plant for the first time.