Objective:To investigate the expression changes of SKOV3 and MRTFA in ovarian cancer and their effects on tumor cell proliferation and invasion.Methods:91 cases of patients with ovarian cancer admitted to our hospital from Jun. 2017 to May. 2019 were enrolled in this study. The relationship between MRTFA mRNA expression and clinical characteristics in ovarian cancer tissues was analyzed. The ovarian cancer SKOV3 cells were cultured and grouped, and the proliferation activity of ovarian cancer SKOV3 cells was detected by MTT. The invasive ability of SKOV3 cells was determined by Transwell method, and the expression of MRTFA protein in SKOV3 cells was determined by Western blot.Results:The relative expression of MRTFA mRNA in ovarian cancer tissues was significantly higher than that in adjacent tissues ( P<0.05) . The relative expression of MRTFA mRNA in ovarian cancer tissues with tumor diameter <10 cm, FIGO stage I-II stage, moderately high differentiation and no lymph node metastasis was lower than that with tumor diameter≥10cm, FIGO stage III-IV stage, poor differentiation, lymph node metastasis in patients with ovarian cancer ( P<0.05) . The absorbance values of ovarian cancer SKOV3 cells in MRTFA intervention group were lower than those in the control group and the negative control group at 24 h, 48 h, 72 h and 96 h ( P<0.05) . Conclusions:The expression of MRTFA is abnormally increased in ovarian cancer. The specific inhibition of MRTFA protein expression can block the proliferation and invasion of SKOV3 cells. The mechanism of action may be related to the transformation process of SRF induced epithelial mesenchyme.