Objective To investigate the expressions of interluekin-3 in BALB/c mice infected by mycoplasma pneumonia (MP)and to clarify the relationship between IL-3 and MP infection.Methods Total 36 BALB/c mice were randomly divid-ed into control group,MP infection group and clarithromycin treatment group.MP infection models were established with the method of dripping cultured MP into nasal cavity of mice.ELISA method was used to assay the levels of IL-3 in the the serum of the mice and bronchoalveolar lavage fluid in various groups.The lung tissue was fixed in formalin for sections,HE staining was performed for the observation of histological changes.Results The MP-infected mouse models were established successfully and MP strains were dripped into the respiratory system of mice.As showed in histopathological observasion re-sults,significantly increased after MP infection and then gradually decreased after clarithromycin treatment.The ELISA re-sults showed that the level of serum and bronchoalveolar lavage fluid of IL-3 of the mice in MP infection group were 96.4± 8.3 ng/L and 60.9 ±6.5 ng/L.Compared with the control group(203.1±14.5 ng/L,96.3±12.6ng/L),serum and bron-choalveolar lavage fluid of mice infected with MP in IL-3 levels were significantly lower (t serum = 19.50,t balf = 17.20,P <0.01),and after treatment with clarithromycin,serum and bronchoalveolar lavage fluid of IL-3 significantly increased,re-spectively(175.6±12.5 ng/L and 85.6± 10.2 ng/L,tserum = 17.41,tbalf = 14.62,P <0.01),the difference was statistically significant.Conclusion IL-3 levels were decreased in mycoplasma pneumonia mice.Clarithromycin treatment had effect on mycoplasma pneumonia infecttion.The expression level IL-3 may be related to the severity degree of MP infection.

Objective To compare the curative effects of extraperitoneal laparoscopic hernia repair and tradi-tional hernia repair in the treatment of inguinal hernia.Methods The clinical data of 200 inguinal hernia patients in our hospital from October 2013 to Novermber 2015 were retrospectively analyzed.They were divided into traditional hernia repair group (100 cases)and laparoscopic hernia repair group (100 cases)according to different surgical treatment.The operation time,bed activity time and hospital stay of the two groups were detected.The uroschesis, hydrocele,incidence of postoperative pain and recurrence rate of the two groups were detected.Results The opera-tion time between the two groups had no significant difference(t =1.74,P >0.05).The bed activity time[(2.6 ± 1.6)h]and hospital stay[(5.7 ±2.1 )d]of the laparoscopic hernia repair group were better than the traditional hernia repair group[(9.1 ±3.3)h,(7.4 ±2.3)d,t =17.72,5.46,all P <0.05].The uroschesis 3.0%,hydrocele 0,incidence of postoperative pain 10.0% and recurrence rate 0 of the laparoscopic hernia repair group were lower than the traditional hernia repair group(21.0%,15.0%,50.0%,12.0%,χ2 =15.17,15.34,39.11,11.69,all P <0.05).Conclusion The recovery is fast in inguinal hernia patients treatment by extraperitoneal laparoscopic hernia repair,complication is little,which is worthy of application.

Objective To study the endoscopic manifestation of gonorrheal proctocolitis.Methods Retrospectively reviewed and analysed the clinical and endoscopic smaples of 6 gonorrheal proctocolitis cases on the findings of clinical endoscopic features,pathology,mucus smear and cultures.Results Colonoscopic features of rectum and distal sigmoid mucosa revealed varying grades of edema,congestion and abundant mucus in 6 cases;supurative secretion in 3 cases;scattered bleeding spots,local erosive and superficial ulceration in 2 cases. Pathological findings were chronic inflammation in 4 cases,local erosion,necrosis with superficial ulceration in 2 cases.Rectum mucus smear and mucus culture were positive for gonococcus in all 6 cases.Conclusion The colonoscopic and pathological features of proctocolitis merely showed the non-specific changes even in the severe cases.The gram stain and culture of rectal mucus,particularly the later,gave the highest diagnostic significance.

AIM: To observe the effects of Egr-1 gene knockout on the expression of inflammatory-related factors in pancreatic tissue in a mouse acute pancreatitis model.METHODS: The experimental pancreatitis was induced by high-dose of cearulein in wildtype mice and Egr-1 knockout mice.The pancreatitis indexes,such as serum amylase,pancreata edema,and myeloperoxidase(MPO) levels in pancreata and lungs were recorded.The mRNA levels of tissue factor(TF),plasminogen activator inhibitor(PAI-1),monocyte chemoattractant protein(MCP-1),Gro-1,IL-6 and ICAM-1 were measured by quantitative PCR.RESULTS: Contrary to wildtype mice,typical pancreatitis was not induced by high-dose cearulein in the Egr-1 knockout mice,not only markedly reduced edema in pancreata and lungs,but decreased MPO levels in lungs as well were found.Furthermore,the mRNA of TF,PAI,MCAP,ICAM-1 and IL-6 in pancreata were significantly decreased in Egr-1 knockout mice.CONCLUSION: The severity of pancreatitis and lung damage is ameliorated in Egr-1 knockout mice stimulated by high-dosage of cearulein,which was probably mediated by decreasing expression of inflammatory-related factors in pancreata,such as TF,PAI,MCP-1,ICAM-1 and IL-6.

Objective To construct and identify an eukaryotic expression plasmid containing rat hepatocyte growth factor(rHGF)gene and rat augmenter of liver regeneration(rALR)gene,so that to provide experimental basis for developing new treatments of hepatic fibrosis.Methods The gene fragments of rHGF and rALR were amplified from recombinant prokaryotic plasmid pUC18-rHGF and pUC18-rALR by polymerase chain reaction(PCR),respectively,then were spliced by overlap extension PCR with a linker,and the fusion gene rHGF-linker-rALR was constructed.The fusion gene was directionally inserted into the eukaryotic expression plasmid pcDNA3.1 between restriction sites of Kpn Ⅰ and Xba Ⅰ to construct the recombinant eukaryotic expression plasmid pcDNA3.1-rHGF-linker-rALR,and the new constructed recombinant plasmid was identified by double restriction digestion and DNA sequencing.Results DNA fragments of 2200 bp and 400 bp were observed after the electrophoresis of products amplified from recombinant prokaryotic plasmid pUC18-rHGF and pUC18-rALR,respectively,which was consistent with the theoretical value.The electrophoresis of fusion gene rHGF-linker-rALR obtained by overlap extension PCR technique showed only a 2 600 bp DNA fragment,which was in accordance with the expected value.Electrophoresis of products of pcDNA3.1-rHGF-linker-rALR digested with Kpn Ⅰ and Xba Ⅰ showed two DNA fragments with 2600 bp and 5400 bp,which were both consistent with the expected value.The sequences were confirmed correctly by DNA sequencing.Conclusion The recombinant eukaryotic expression plasmid pcDNA3.1-rHGF-linker-rALR is successfully constructed,which provides experimental basis for developing gene therapy of hepatic fibrosis.

Objective To investigate the safety and clinical effect of prophylactic ileostomy using right lower abdominal specimen extraction incision in laparoscopic rectal low anterior resection and its impact on ileos-tomy closure.Methods A retrospective analysis was conducted on 127 patients who underwent laparoscopic low anterior resection of rectal cancer combined with prophylactic ileostomy in Department of General Surgery in our hospital from January 2020 to December 2023.Patients were divided into observation group(n=60)and control group(n=67)based on whether with prophylactic ileostomy using the right lower abdominal specimen extraction incision or not.Relevant data and clinical data of ileostomy closure were compared between the two groups.Results(1)There was no significant difference in baseline clinical data between the two groups(P>0.05).(2)In terms of operation time,pain score within three days after surgery and postoperative first exhaust time,the observation group was better than the control group,showing statistical difference(P<0.05).In the observation group,the average length of the incision was longer than that in the control group,and it was significantly different between the two groups(P<0.05).There was no significant difference between the two groups in terms of blood loss,post-operative feeding time and postoperative hospital stay(P>0.05).(3)There was no significant difference between the two groups in postoperative and ostomy-related complication S(P>0.05).(4)The observation group was better than the control group in terms of postoperative pain score and postoperative first exhaust time,indicating statistical significance(P<0.05).There were no significant differences in surgical time,abdominal adhesion,blood loss,postoperative hospital stay and postoperative complications between the two groups(P>0.05).Conclusion The patients undergoing laparoscopic rectal low anterior resection who needs prophylactic ileostomy using right lower abdominal specimen extraction incision can improve the appearance of the incision,shorten operation time,reduce postoperative pain of the patients but does not increase postoperative complications and the difficulty of ileostomy closure.It is safe and feasible,which worthy for clinical promotion.

This study explores the effects of enhancing the definitive hematopoiesis(DH)signals during the differentiation of human embryonic stem cells(hESCs)into hematopoietic stem/progenitor cells on the generation efficiency and effector function of natural killer(NK)cells generated from hESCs(also known as hESC-NK cells).The hESC(H1)were transformed into embryoid bodies(Ebs)by centrifugation,and during the induction of K562,were used to analyze the efficiency of hematopoietic differentiation,the efficiency of NK cell generation from hESC,the in vitro effector functions,and the expression of effector function related surface receptors.Compared to the control group,the DH group had a significant increase in the number of arterial hematopoietic endothelial cells(CD34+DLL4+)and a significant decrease in primitive hematopoietic related cells(CD34-CD43+)on day 8 of hematopoietic differentiation(P<0.05).On day 28 of NK cell differentiation,the DH group demonstrated a significant increase in the number of NK cells(CD45+CD56+),while a slight increase in the expression of effector function-related molecules such as IFN-γ,Granzyme B,Perforin and CD107a without statistical significance.Furthermore,the activation receptors CD16a and CD69 were significantly increased,NKP46 was significantly decreased,the inhibitory receptor NKG2A was significantly increased,while CD96 was significantly decreased on hESC-NK cells of DH groups(P<0.05).Conclusively,enhancing the signals for definitive hematopoiesis during hESC differentiation into hematopoietic stem/progenitor cells significantly improves the yield of NK cells and the expression of CD16a without affecting their in vitro effector functions.Our study provides a new approach to improving the efficiency of hESC-NK cell or iPSC-NK cell generation.