ObjectiveTo evaluate the effect of combined gene transfection with p53,p16 and p21 on the growth of human liver cancer cell lines.MethodsAfter transducing adeno associated virus(AAV) mediated p53, p16 or/and p21 genes to human hepatic carcinoma cell lines HLE,HepG2,QGY 7701,QGY 7703,BEL 7402,SMMC 7721, gene expression and tumor inhibition were studied in vitro and in BALB/c mouse model.ResultsAdeno associated virus mediated p53, p16 or p21 encoding gene could express in BEL 7402 cell line. Each individual type of recombinant AAV effected a significant induction of tumor cell apoptosis both in vitro and in vivo, the rate of apoptotic cells in vitro is about 30% and that of tumor growth inhibition is about 30～44%. And the apoptosis inducing efficiency was the highest after the tumor cell line was transfected by three recombinant AAV simultaneously, with a rate of 56% (in vitro) and 65% (in vivo).ConclusionNot only could all of the exogenous wild type p53, p16 and p21 genes mediated by AAV inhibit the growth of liver cancer cell lines, but also can the efficiency be significantly elevated by combined gene transfection.

Objective To investigate the relationship of PTEN,Ki-67,type of credits of thymoma in WHO organization and Masaoka staging,and the significance of the biology's expression in thymoma.Methods The expression of PTEN and Ki-67 in 52 pieces of thymoma specimens was detected by the method of immunohistochemical SP.Results Positive expression rate of the 52 patients with thymoma was decreased gradually after WHO classification (8/10,10/14,6/12,4/11,0,0 and 10/13,12/20,6/16,0),Masaoka staging and PTEN.The difference were significant in statistics (λ2 =11.751,P =0.038 ; x2 =8.311,P =0.040).Positive expression rate of Ki-67 was gradually increased (1/10,6/14,6/12,8/11,2/3,2/2 and 3/13,9/20,10/16,3/3),the difference was also significant in statistics (x2 =11.231,P =0.047; x2 =7.904,P =0.048).Conclusions The positive expression rate of PTEN,Ki-67 are both related to clinical classification and staging closely.The detection to the positive expression rate of PTEN and Ki-67 can contribute to judging thymoma biological characteristics,thus,it will be helpful in diagnosing the thymoma,analyzing the degree of malignancy,invading and transferring ability.And then provide objective indicators for clinical diagnosis and treatment.

The focal hepatic lesion caused by local injection of absolute alcohol in rats was evaluated with ultrasonic contrast agent and pathologic examination. Twenty adult Wistar rats weighing about 200 g were injected with absolute alcohol (0.05-0.1 mL each one) on the exterior left lobe of the liver under the monitoring of ultrasound. Pulse inversion harmonic imaging was used to evaluate the focal lesion after bolus injection of ultrasonic contrast agent (0.05 mL/200 g) through caudal vein. Seven days later, the focal lesion was studied again as before. The exterior left lobe of liver with focal lesion was incised and underwent pathologic examination. The results showed that all of the focal lesions could be defined clearly after bolus injection of the ultrasonic contrast agent under the mode of pulse inversion harmonic imaging. There was good correlation between the size of the focal lesion measured by ultrasound on the 7th day after the "ablation" under the mode of pulse inversion harmonic imaging and that gotten by pathologic examination (P = 0.39). The focus size measured by ultrasound right after the ablation was larger than that gotten by pathologic examination (P = 0.002). It was concluded that ultrasonic contrast agent plus pulse inversion harmonic imaging could be used to assess the size of the focal hepatic lesion caused by local injection of absolute alcohol in rats.

Salsola collina is widely distributed in droughty and semi-droughty area, which is used as a kind of folk remedy in traditional Chinese medicine for treatment of hypertension. The study is on the chemical constituents of this herb from its aerial parts to obtain its active constituents. Dried and crushed aerial parts of this herb were extracted three times with 95% EtOH at reflux. The ethanol extracts were combined and concentrated under reduced pressure at 70 ℃ to yield residue, which was suspended in water and successively partitioned with light petroleum, chloroform and n-butanol. The chloroform and n-butanol fractions were treated by various chromatographic techniques, such as silica gel, C18 reversed-phase silica gel and macroporous resin column chromatography. Compounds were elucidated by their physicochemical properties and spectroscopic analysis. In the course of our study on searching biological active components from this herb, a new alkaloid together with three known alkaloids were isolated and identified as N-transferuloyl-3-methyldopamine (1), 3-[4-(β-D-glucopyranosyloxy)-3- methoxyphenyl]-N-[2-(4-hydroxyl-3-methoxyphenyl)ethyl]-2-propenamide (2), salsoline A (3), salsoline B (4). Compound 4 is a new compound and named as salsoline B, while compound 2 was obtained in Salsola collina for the first time.

A new phenolic glycoside was isolated from the spikes of Prunella vulgaris. Its structure was elucidated as gentisic acid 5-O-beta-D-(6'-salicylyl)-glucopyranoside by spectroscopic evidence and chemical analysis.

AIM: To evaluate the effect of simvastatin on the left ventricular mass in patients with essential hypertension (EH). METHODS: 50 patients with hypertension without severe complication were randomly divided into two treatment groups: combination treatment group and hydragogue group, and 25 normal subjects without any treatment were taken as the control. The 25 patients in combination treatment group were given simvastatin and hydragogue for 12 weeks while the other patients in hydragogue group were given hydragogue during the same time. The left ventricular mass was examined from ultrasonography in all patients before and after treatment. RESULTS: The left ventricular mass index (LVMI) was higher in the two treatment groups of patients (133.61±31.02, 118.04±39.62 g·m-2) than that in the control group (88.79±22.73 g·m-2) before treatment (P<0.01, 0.0001, respectively) while the blood pressure was higher. There was no significantly difference in age, serum concentrations of total cholesterol or triglyceride, sugar and blood pressure between the two treatment groups and the control group (P>0.05). There was no significant difference in all variables between the two treatment groups before treatment. After treatment, the LVMI was decreased (133.61±31.02 vs 91.07±16.01 g·m-2, P<0.01) in the combination treatment group while there was no significant change in LVMI in the hydragogue group compared with the control group. The blood pressure in the two treatment groups was decreased to the normal. Compared with hydragogue group, the change of LVMI was higher in the combination group though the serum concentrations of total cholesterol, triglyceride or sugar were not significantly different. No significant change in serum concentrations of total cholesterol, triglyceride or sugar was found during treatment in the two groups. The change of LVMI did not correlate with the change of blood pressure, serum concentrations of total cholesterol , triglyceride or sugar in the combination treatment group(P>0.05). CONCLUSION: Being independent of the changes of serum concentrations of total cholesterol, triglyceride or sugar and blood pressure, simvastatin can inhibit the increase of left ventricular mass in patients with essential hypertension.

BACKGROUND:Tumor necrosis factor-α-induced protein-8 like-2 (TIPE2), an anti-inflammatory protein, through the T cel receptor (TCR) and TOLL-like receptor signaling pathway, implements negative regulation of adaptive immunity and innate immunity, and thus effectively maintains the stable internal environment of the body.
OBJECTIVE:To construct a recombinant adenovirus that can overexpress rat TIPE2 gene.
METHODS:TIPE2 cDNA target gene was amplified from rat’s lymphocytes using RT-PCR, cloned into shuttle plasmid pShuttle-clontech, and then subcloned into artificial adenovirus vector AdC68. Hereafter, HEK 293 cel s were transfected to generate a recombinant adenovirus. HEK293A cel s were infected using this recombinant adenovirus, and then TIPE2 gene level was tested by western blot method.
RESULTS AND CONCLUSION:Based on results of PCR, digestion identification and sequencing, the obtained cDNA was the coding sequence region of TIPE2. Western blot findings showed that the recombinant adenovirus could overexpress TIPE2 gene. These findings indicate that the recombinant adenovirus is constructed successful y and can express TIPE2 gene stably.

Objective To assess the aorta elastic properties in the procession of atherosclerosis by ultra‐high frequency ultrasound ,and to detect the relationship between the aorta elastic properties and the atherosclerotic plaque burden .Methods Mice deficient for the apolipoprotein E (ApoE‐/‐) with high‐cholestrol diet were studied as an age‐dependent model of atherosclerosis .At 8 ,16 ,24 and 32 weeks of age , the blood pressure in the ascending aorta was measured by catheter ,and the aorta mechanical properties were assessed by measuring aortic elastic modulus of the ascending aorta with ultra‐high frequency ultrasound .The plaque burden was assessed by high‐frequency ultrasound and Masson′s trichrome stain , separately .Results Vessel thickness at the lesion‐prone sites of the lesser curvature of the aorta and the proximal brachiocephalic artery increased with age ,consistent with the Masson′s trichrome staining which showed age‐dependent worsening of atherosclerosis in the mice model .Elastic modulus of the aorta significantly increased from 8 to 32 weeks of age in E‐/‐mice .There was a statistically difference between any two groups .Conclusions With the progression of atherosclerosis and the increased plaque burden ,aorta mechanical properties deteriorated in Apo E‐/‐mice .Ultra‐high frequency ultrasound was a potential tool for assessment of plaque burden and aorta mechanical properties in mouse model .

Objective To investigate the expression of tumor-associated carbohydrate antigen sTn in breast neoplasm,and analyzed the association between the expression of sTn and size of tumor,age,menopause,axillary lymph node metastasis,clinical stage,pathologic grade and the level of estrogen receptor in breast carcinoma.Methods The monoclonal antibody TKH-2 was used to detect the immunohistochemical expression of sTn in 46 cases of breast carcinoma,4 breast adenoma and 2 gynecomastia.The level of estrogen receptor was detected by S-P method in 46 breast carcinoma.Results Immunohistochemical expression of sTn was not detected in breast adenoma and gynecomastia.The expression of sTn was found in 28 of 46 breast carcinoma(60.9%).The expression of sTn was significantly associated with axillary lymph node metastasis(P0.05).In 46 breast carcinoma,the positive quantity of estrogen receptor was 25(54.3%).Conclusion STn immunostaining appears to be a poor prognostic factor in patients with breast carcinoma.It is more useful in detecting the expression of sTn together with the level of estrogen receptor in breast carcinoma to investigate the prognosis of the patients.