Purpose To investigate the relationship between Helicobacter pylori L-form ( Hp-L) infection and expression of XPF and p53 in gastric carcinoma. Methods XPF, p53 and Hp-L were examined in 239 cases of gastric carcinoma and healthy individuals by means of Giemsa stain and immunohistochemical staining with ABC method. Results XPF and p53 expression in gastric carcinoma was significantly higher than that of the surrounding tissue (P<0. 01). There were positive correlation between the expression of XPF and p53 (r=0. 196, P<0. 01). The expression of XPF was related to the Laurén classification (P<0. 05). The expression of p53 was associated to the depth of invasion (P<0. 05). (2) 175 (73. 22%) cases were Hp-L positive by both Giemsa and immunohisto-chemical staining. Hp-L expression in gastric carcinoma were significantly higher than that of the surrounding tissue (P<0. 01). (3) In the Hp-L positive group, XPF and p53 were significantly higher than in that of the Hp-L negative group (P<0. 01). Positive corre-lation existed between positive Hp-L staining and the expression of XPF and p53 (r=0. 32, r=0. 296, P<0. 01). (4) Univariate a-nalysis showed that depth of invasion, distant metastasis, TNM stage, XPF and p53 were related to the prognosis of gastric carcinoma ( P<0. 05). Multivariate Cox model analysis showed that XPF was an independent prognostic factor for the patients (P<0. 05). The pa-tients with positive expression of XPF had a shorter survival time. When combined with the depth of invasion and distant metastasis, the correction risk increased by 3. 791 times. Conclusion XPF, p53 and Hp-L may play very important roles in the development of gastric carcinoma. The high expression of XPF implies poorer prognosis. Hp-L infection may be related to the up-regulated expression of XPF and p53.

Objective To observe the tissue displacements between the acupoints and non-acupoints using tissue Doppler imaging.Methods Acupuncture bilaterally were performed at Zusanli,Qvchi and corresponding control points 1 cm lateral to them in 30 healthy human subjects.Monitored by the dynamic system for detecting the force of acupuncture needle during the acupuncture process,tissue Doppler imaging was recorded on the condition of keeping the depth of needle insertion and the rotational torque at the same level approximately.Tissue Doppler imaging was also recorded before and after the acupuncture.Results The mean tissue displacements of the aforementioned 2 acupoints were all significantly greater than those of the corresponding non acupoints(P＜0.05).The mean tissue displacements after the acupuncture were all significantly greater than those before the acupuncture(P＜0.001).Conclusions Tissue Doppler imaging provides a new thought and method for the research of the acupoints.

Objective To analyze the antibody titer value of indirect haemagglutination test(IHA)in 135 confirmed acute schistosomiasis patients,so as to provide the evidence for improving the diagnosis and treatment of acute schistosomiasis. Meth-ods A total of 135 acute schistosomiasis inpatients were selected from 2001 to 2006. They all received the IHA antibody titer de-tection,and the correlation among the age,incubation period,and hospitalization days was calculated. Results The antibody titers of IHA were higher than 1:320 in all the cases. The percentages of 1:640,1:1280,1:2560,1:5120 and 1:10240 were 1.48%,28.15%,35.56%,20.00%,and 14.81%respectively. The mean age was(47.70 ± 14.58)years,average incuba-tion period was(38.03 ± 4.59)days and mean hospital stay time was(15.08 ± 3.79)days. The antibody titer value had no corre-lation with the age distribution(r=0.109,P>0.05). There was a negatively correlation between the antibody titer value and in-cubation period,(r=-0.558,P<0.01),there was a positive correlation between the antibody titer value and hospitalization time(r=0.791,P<0.01),and there were significant differences among different groups(F=17.07,64.53,both P<0.01). Conclusions The antibody titer of acute schistosomiasis cases detected by IHA is 1:640 and above. There is no correlation be-tween the antibody titer value and age,but the antibody titer value is higher,the incubation period is shorter and hospitalization time is longer.

Objective To obtain the shear wave velocity(SWV) in breast tumors at different probe orientations.Methods SWV was measured on 92 breast tumors in 48 female patients with the probe placed on transverse,longitudinal and 45 degree planes.Pathological test results were followed up.Results Pathological tests showed benign tumors in 48 patients (92 tumors).Of the three planes,values of the biggest and the smallest SWV showed significant difference (P ＜0.001).Conclusions SWV differs on different planes of breast tumors.So the effect in different probe orientations should be considered using SWV for the differential diagnosis of breast tumors.

Objective To evaluate the value and characteristics of left ventricular global systolic strain and its relation to the left ventricular global systolic function in patients with myocardial infarction(MI) by three-dimensional speckle tracking echocardiography (3D-STI).Methods In 24 patient subjects and 20 control subjects,the myocardial motion was tracking by 3D-STI,left ventricular ejection fraction(LVEF),and global longitudinal peak systolic strain(LVGLS),global circular peak systolic strain(LVGCS),global radial peak systolic strain(LVGRS),global area peak systolic strain(LVGAS) were measured.The values were compared between the two groups,the correlations between LVEF and LVGLS,LVGCS,LVGRS,LVGAS were analyzed respectively.Results (1)There was significant difference of each index about LVEF and global strain between normal group and MI group (all P ＜0.05).(2)The correlations among LVEF and LVGLS,LVGCS,LVGRS,LVGAS were found (r =-0.626,-0.770,0.772,-0.748 respectively,P ＜0.01 for all).(3) Bland-Altman analysis showed there were good agreements in both patients with MI and control subjects.Conclusions 3D-STI could be applied non-invisibly and objective to assess alteration of myocardial global systolic function by accurately measuring strain.Therefore,3D-STI appears to be a reliable and useful tool to estimate the left ventricular systolic function of MI.

Objective To study the microcirculation pattern of expanded skin and its influence on skin blood supply. Methods The microvessel diameter, blood velocity and blood volume were recorded under the microcirculatory imaging system and the microvessel density was measured by enzyme histochemical staining and medical imagine analysis. Results Compared with the control group, all the recorded values rose obviously in every expanded groups ( P

Objective To investigate the angiogenic effect of leptin on grainy fat transplantation. Methods Grainy fat was obtained from the Wistar rat subcutaneous tissue in the abdomen. It was then mixed with 0.9 % NS and 20 ng/ml leptin and transplanted in the head subcutaneously. The transplanted tissue was taken out after 10, 20 and 40 days and was stained with AKP histochemistry and actin immunohistochemistry. The microvessel density was measured by medical imagine analysis system. The data were analyzed with SPSS software. Results The values of microvessel density in leptin groups were 0.12 ?0.04?m 2/?m 2 after 10 days and (6.83?1.86)?10 -2 ?m 2/?m 2 after 20 days. This was obviously high as compared with (1.49?0.88)?10 -2 ?m 2/?m 2 and (4.32?2.11)?10 -2 ?m 2/?m 2 in saline groups in the same observing periods ( P

Objective To describe an appreciate operation method in the treatment of deformi-ties secondary to gluteal muscles and fascia contracture. Methods 25 cases of serious secondary de-formity of gluteal muscles and fascia contracture were treated by Z-plasty. The contracted fascia and scar were loosed and the skin around the wound was rearranged. Results The following-up time was from 6 months to 3 years with an average time of 1.6 years. All cases rehabilitated to normal or nearly normal in gait. Aesthetic results were also satisfactory. No recurrence was observed. Conclusion Re-arranging the contracted fascia with Z-plasty can extend the fascia and improve the movement of the hip joint. Besides, Z-plasty treatment of skin around the scar is benefit for the aesthetic result of the deformities, Z-plasty of contracted fascia and skin is an appreciate operation method in the treatment of deformities secondary to gluteal muscles and fascia contracture.

Objective To observe the clinical effect of a silicone tractor in the treatment of nipple inversion. Methods 15 patients were treated with this new type of tractor. Of all the patients, 11 were with mild to moderate nipple inversion, and 4 were serious affected patients. In the seriously affected group, the tractor was used after operation. Patients in the mild to moderate nipple inversion group was treated with the silicone tractor only. Results The result in all the patients was satisfactory after follow-ing-up for 6 to 12 months. The appearance of the nipples was well. Conclusion Continuously pulling with silicone tractor is an ideal method in nipple inversion treatment. It is simple, safe and effective.

ObjectiveTo explore the effect of mental toughness level on positive and negative emotion and mental health in children with epilepsy.MethodsIn accordance with the International League against epilepsy and epilepsy syndrome in 1989 International Classification of young children with epilepsy,160 cases were sampled.Based on the Adolescent Resilience Scale ( HKRA ) score,according to the 27％ principles of delimitation,the patients were divided into high HKRA score group and low HKRA score group,43 cases in each group.Then positive emotions and negative affect scale (PANAS),mental health test (MHT) were applied to two groups.Results,Except family support dimension,the others HKRA dimension and total score were higher in female children with epilepsy than those in male patients,there was significant difference (P＜ 0.01 ).The positive affect score were higher in high HKRA score group than that of low HKRA group ; and negative emotion was significantly lower than that in low HKRA group (P＜ 0.01 ).Except physical symptom dimension,other dimensions and total score of HKRA in high HKRA score group were higher than those in low HKRA group,there was significant difference (P＜ 0.01 ).Children with epilepsy HKRA score was positive correlation with positive emotion score but negative correlation with negative affect scores (P ＜ 0.01 ) ; in addition to physical symptom,HKRA score and MHT score,and all the other dimensions were negatively related (P＜0.01 or 0.015).ConclusionThe mental toughness level are significantly related to children's epilepsy patients with positive and negative emotion and mental health status.A high level of mental toughness can increase positive emotional experience,and reduce the negative emotional experience of children with epilepsy.