1.Triple staining of immunohistochemistry.
You-zhi YU ; Min LIN ; Wei-cheng XUE ; Qiu-jing SONG ; Dan-hua SHEN
Chinese Journal of Pathology 2005;34(4):244-245
4.Dynamic inhalation contamination installation in evaluation of detection of toxicity of liquid chemicals.
Jing WANG ; You-run YANG ; Wei-lin FAN ; Yong-min MA ; Xin LI ; Ying-hua LIU
Chinese Journal of Industrial Hygiene and Occupational Diseases 2006;24(7):435-436
Administration, Inhalation
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Animals
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Female
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Male
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Pesticides
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analysis
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toxicity
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Rats
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Rats, Wistar
5.Status assessment on rural environmental hygiene in Pukou District of Nanjing in 2017
Jing ZHANG ; You-huan SHI ; Hu-min ZHU ; De-lin LI ; Jun YOU ; Lei QING
Shanghai Journal of Preventive Medicine 2020;32(2):171-
Objective To ascertain the level and dynamic changes of environmental health hazards in the rural areas of Pukou, Nanjing, to objectively evaluate the situation of rural environmental sanitation, providing basis and support for formulating policies and measures by the higher authorities. Methods According to the requirements of the rural environmental sanitation monitoring program of Jiangsu Province in 2017, 5 streets, 20 administrative villages were selected as monitoring points and 100 families used as monitoring objects.The monitoring data were obtained through interviews, access data, field records and laboratory tests. Results Centralized water supply was realized by 20 monitoring points.The rate of collection and centralized disposal of domestic waste was 95.00%.The sewage disposal sites were mainly discharged into sewage treatment plants and harmless sanitary toilets, accounting for 41.00% and 42.00% respectively.The coverage rate of sanitary toilets was 95.54%.Those who did the work of killing rats, flies and mosquitoes accounted for 40.00% and those who did the work of killing cockroaches 25.00%.And 98.00% of the families used fuel for gas, 98.00% of the families never burned straw in the fields, 88.00% of the families did not use the film, and the membrane recycling rate was 91.67%.It was found that 100% of the households drank boiled water, and 90.00% of the respondents developed the habit of washing hands before and after meals.The detection rate of Ascaris eggs was 20.00% in 20 soil samples, and the contents of Pb, CD and Cr were all within the normal range. Conclusion Although some achievements have been made in the rural environmental sanitation in Pukou, the management of pollution of rural sewage, waste and feces should be further strengthened, and the dangerous factors that affect the health of rural residents should be decreased and four pests removal activities actively carried out.
6.Effect of guilu erxianjiao in suppressing splenic T-lymphocyte apoptosis in mice undergoing chemotherpy.
Sheng-You LIN ; Min-He SHEN ; Jian CHEN
Chinese Journal of Integrated Traditional and Western Medicine 2008;28(4):339-342
OBJECTIVETo investigate the effect of Guilu Erxianjiao (GLEXJ) in suppressing chemotherapy induced cell apoptosis in mice and to discuss its possible acting mechanism.
METHODSSeventy-two tumor-bearing Kunming mice were randomly divided into 6 groups, Group A, treated with normal saline; Group B treated with cyclophosphamide (CTX), Group C treated with Chinese herbal medicine, and Group D-F, the three combined treated group received CTX plus high, moderate and low dose GLEXJ. All mice were sacrificed after 9-day medication, their splenic tissues were taken for determining T-lymphocyte apoptosis with flow cytometer, the expression of bcl-2 mRNA and Caspase-3mRNA detected by RT-PCR.
RESULTSAs compared with Group A, FITC+/PI- cell proportion of spleen T-lymphocyte cells and Caspase-3mRNA expression were higher, while bcl-2 mRNA expression was lower in Group B; as compared with Group B, the former two indexes were lower and the latter was higher in the three combined treated groups (all P <0.05).
CONCLUSIONGLEXJ could efficiently suppress the splenic T-lymphocyte apoptosis in tumor bearing mice undergoing chemotherapy, one of its mechanisms may be through up-regulating of bcl-2 mRNA expression and down-regulating of Caspase-3mRNA expression.
Animals ; Apoptosis ; drug effects ; Caspase 3 ; genetics ; metabolism ; Cell Proliferation ; drug effects ; Drugs, Chinese Herbal ; administration & dosage ; Humans ; Male ; Mice ; Neoplasms ; drug therapy ; genetics ; metabolism ; physiopathology ; Proto-Oncogene Proteins c-bcl-2 ; genetics ; metabolism ; Random Allocation ; Spleen ; cytology ; drug effects ; metabolism ; T-Lymphocytes ; cytology ; drug effects ; metabolism
7.Analysis and Development Prediction of Current Situation of TCM Human Resources
Yue XU ; Bao-Lin HONG ; Zong-You LI ; Yan-Min HU
Chinese Journal of Information on Traditional Chinese Medicine 2018;25(6):1-5
Objective To understand the current situation of TCM human resources; To forecast the number of TCM human resources in 2020; To provide data for national scientific management policy. Methods Through Statistical Yearbook of China's Health and Family Planning and Yearbook of Chinese Traditional Medicine, the national human resources data of TCM were collected and compiled from 2010 to 2015, and descriptive analysis and gray model method were used for data analysis. Results The TCM human resources continued to grow, but the growth rate of Chinese pharmacists was slow; the lack of TCM human resources in TCM health institutions; the uneven distribution of human resources in inter-provincial TCM; the number of TCM human resources in 2020 could meet the requirements of TCM 13th Five-year Plan. Conclusion The development of Chinese pharmacists should be paid attention to; the proportion of TCM pharmacists should be balanced; the proportion of TCM human resources in TCM institutions should be improved; scientific planning and the coordinated development of TCM human resources in various regions should be promoted.
8.Relationship among ALDH2 gene polymorphism, alcohol metabolism and acetaldehyde level in peripheral blood.
Hui XIONG ; Wei WANG ; Yi YE ; You-Yi YAN ; Min XIAO ; Ruo-Yun RUAN ; Lin-Chuan LIAO
Journal of Forensic Medicine 2014;30(1):31-35
OBJECTIVE:
To explore alcohol pharmacokinetics as well as acetaldehyde level in peripheral blood in human subjects with different ALDH2 genotypes after drinking.
METHODS:
Venous blood samples of 14 unrelated volunteers were collected. Polymerase chain reaction-restriction fragment length polymorphism technology was adopted for DNA extraction and ALDH2 genotyping. The volunteers were asked to drink beer at certain doses. The concentration of alcohol and acetaldehyde were assayed by headspace gas chromatography method at different time. The pharmacokinetic parameters were calculated.
RESULTS:
According to the results of electrophoresis, 5 people carried ALDH2*1/*1 as wild group and 9 people carried ALDH2*1/*2 as mutation group. The good linear range of alcohol and acetaldehyde were 0-1 570.7 microg/mL and 0-5.1772 microg/mL, respectively. The AUC values of alcohol and acetaldehyde and the t1/2Z value of alcohol were higher in the mutation group than that in the wild group. But the CL/F value of alcohol was lower in the mutation group than that in the wild group (P<0.05).
CONCLUSION
After the consumption of alcohol, alcohol and acetaldehyde metabolism in blood slow down in ALDH2*1/*2 mutation group influenced by the inhibition of enzyme activity, leading to the accumulation of acetaldehyde in peripheral blood, thus reinforcing their effects in the body.
Alcohol Drinking
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Aldehyde Dehydrogenase/genetics*
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Aldehyde Dehydrogenase, Mitochondrial
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Ethanol/metabolism*
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Genotype
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Humans
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Polymerase Chain Reaction
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Polymorphism, Genetic
9.Anti-atherosclerosis role of N-oleoylethanolamine in CB2.
Ya-Ting GAI ; Qiang SHU ; Cai-Xia CHEN ; You-Lin LAI ; Wen-Jun LI ; Lu PENG ; Li-Min LIN ; Xin JIN
Acta Pharmaceutica Sinica 2014;49(3):316-321
To observe a PPAR-alpha agonist effect of N-oleoylethanolamine (OEA) on CB2 (cannabinoid receptor 2), an anti-inflammatory receptor in vascular endothelial cell, healthy HUVECs and TNF-alpha induced HUVECs were used to establish a human vascular endothelial cell inflammatory model. Different doses of OEA (10, 50 and 100 micromol x L(-1)) had been given to HUVECs, cultured at 37 degrees C for 7 h and then collected the total protein and total mRNA. CB2 protein expression was detected by Western blotting and CB2 mRNA expression was assayed by real-time PCR. As the results shown, OEA (10 and 50 micromol x L(-1)) could induce the CB2 protein and mRNA expression, but not 100 micromol x L(-1). To detect if anti-inflammation effect of OEA is partly through CB2, CB2 inhibitor AM630 was used to inhibit HUVEC CB2 expression, then the VCAM-1 expression induced by TNF-alpha was detected, or THP-1 adhere to TNF-alpha induced HUVECs was examined. OEA (50 micromol x L(-1)) could inhibit TNF-alpha induced VCAM-1 expression and THP-1 adhere to HUVECs, these effects could be partly inhibited by a CB2 inhibitor AM630. The anti-inflammation effect of OEA is induced by PPAR-alpha and CB2, suggesting that CB2 signaling could be a target for anti-atherosclerosis, OEA have wide effect in anti-inflammation, it may have better therapeutic potential in anti-inflammation in HUVECs, thus achieving anti-atherosclerosis effect.
Anti-Inflammatory Agents
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pharmacology
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Atherosclerosis
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pathology
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Cell Adhesion
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drug effects
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Cells, Cultured
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Endocannabinoids
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pharmacology
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Endothelial Cells
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cytology
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metabolism
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Ethanolamines
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pharmacology
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Humans
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Indoles
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pharmacology
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Monocytes
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drug effects
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Oleic Acids
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pharmacology
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PPAR alpha
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antagonists & inhibitors
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RNA, Messenger
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metabolism
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Receptor, Cannabinoid, CB2
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antagonists & inhibitors
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genetics
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metabolism
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Tumor Necrosis Factor-alpha
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pharmacology
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Vascular Cell Adhesion Molecule-1
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metabolism
10.Experimental study on p53 gene mutation in keloid fibroblasts.
Wang LIU ; You-Hui JIANG ; You-Liang LI ; Zi-Hao LIN ; Hua JIANG ; Qian TAN ; Jian-Lin ZHANG ; Zi-Min WANG
Chinese Journal of Burns 2004;20(2):85-87
OBJECTIVETo explore the significance and the role of the p53 gene mutation in the exon 4 to 8 in keloid fibroblasts.
METHODSTissue samples from twelve patients with keloid and twelve hyperplastic scar respectively were harvested for in vitro culture of fibroblasts, and normal skin samples from the same patients were employed as the control. Polymerase chain reaction-based single-strained conformational polymorphism (PCR-SSCP) and DNA sequencing were employed to detect p53 gene mutations of the fibroblasts.
RESULTSThe points and frameshift mutations in the exon 4, 5, 6, 7 of p53 gene were identified in 9 of the 12 keloid tissue samples. No p53 gene mutation was detected in all hyperplastic scar and normal skin samples.
CONCLUSIONp53 gene mutation might play an important role in the formation and development of keloids.
Female ; Fibroblasts ; metabolism ; Genes, p53 ; Humans ; Keloid ; genetics ; Male ; Mutation