Objective To set up an animal model of high altitude pulmonary edema(HAPE) by putting SD rats in the condition simulating high altitude where is hypoxic and cold,in addition the SD rats are forced to swim.Methods SD rats were divided into normal control group and experiment group(SD rats were put into the stimulated 4 000-meter low pressure chamber;Temperature: 8:00-20:00 10 ℃,20:00-8:00 4 ℃;Everyday the rats were forced to swim 6 times a day,once every 4 hours,each for 40 min).The rat lung arterial pressure was observed and pulmonary tissue was collected for pathological examination at 24,48,72 h.Results PaO_(2),SaO_(2),_()PaCO_(2) in the experiment decreased significantly.Lung arterial pressure increased significantly at 72 h,consistent with the progression of clinical HAPE.About 70.2% rats in the experiment exhibited swollen and thicken pulmonary tissue,the smaller alveolar cavity,but no case of alveolar edema was found.Conclusion The animal model of HAPE is successfully replicated,but the alveolar model of HAPE needs further study.

In multilayer spiral CT heart and coronary artery imaging, image data are acquired by ECG gating technology when heart beats slowest so as to restrain pulsation pseudo-image. ECG gating technology includes foresight ECG gating and retrospective ECG gating. The principle, methods and mutual image reconstruction are introduced in this paper.

Objective To compare analgesic efficacy of intravenous postoperative and preemptive analgesia with flurbiprofen axetil combined with small dose of morphine and with morphine alone. Methods One hundred and twenty patients were randomly divided into four groups: group A,morphine 30 mg +0.9% NaCl for postoperative analgesia,n=30;group B,morphine 20 mg +0.9% NaCl for postoperative analgesia,n=30;group C,flurbiprofen axetil 100 mg + morphine 20 mg +0.9% NaCl for postoperative analgesia,n=30;group D,flurbiprofen axetil 100 mg before operation + morphine 20 mg +0.9% NaCl for postoperative analgesia,n=30.The drugs in each group were diluted to 100 mL and infused by a pump at a rate of 2 mL/h with a patient-controlled analgesia(PCA)bolus of 2 mL after a loading dose of 5 mL.The visual analogue scale(VAS),demanding times for PCA and incidence of side effects were recorded during the period of postoperative 24 h. Results The VAS of group B at 3 h after operation was significantly higher than those of the other three groups(P

Epidemiologic Studies ; Humans ; Neoplasms ; epidemiology

Objective To study the effects of hyperbaric oxygen therapy on traumatized cerebral neurons and investigate its mechanism in attenuating cerebral damage.Methods Seventy-two rats were randomly assigned to a control group,a traumatic brain injury group or a traumatic brain injury group treated with hyperbaric oxygen. Each group was observed 1,7 and 24 days after the operation.TUNEL was used to examine the distribution of apop- tosis cells.An immunohistoehemical method was used to examine the distribution of Bcl-2 and Bax immunoreactive- positive cells in the brain tissues.Results The average percentages of both apoptosis cells and Bcl-2 immunoreac- tive-positive cells were lower in the CAl region of the traumatized brains treated with hyperbaric oxygen than in those of the traumatic injury group at each time of observation.A significant difference in Bax immunoreactive-positive cells between the two groups was also observed.Conclusion Hyperbaric oxygen therapy can significantly protect neurons against traumatic brain injury and modulate the expression of the apoptosis related genes Bcl-2 and Bax.This may ex- plain the protective mechanisms of hyperbaric oxygen therapy in treating traumatic brain injury.

Objective To investigate the diagnostic value of serum soluble transferrin receptor(sTfR) in children with iron deficiency anemia(IDA).Methods Sixty-three children with microcytic hypochromic anemia were divided into IDA and non-IDA(n-IDA)groups,on which sTfR and other iron metabolism related indexes such as serum ferritin(SF) and serum iron(SI) were measured,and(t-test) between groups and analysis of ROC curve were carried out.Results The mean concentration of sTfR in the group of IDA was above normal value and t-test difference was extremely significant compared with n-IDA group(P

Hot melt extrusion (HME) is an effective method to make the drugs form amorphous solid dispersions (ASD) inmicrostructure, through which we can improve the dissolution behavior so as to improve the bioavailability ofhydrophobic drugs This review mainly focuses on the. screening of active pharmaceutical ingredients and polymer carriers, feasibility evaluation of the technique, and the control of key parameters of preparation process, summarizes the application of HME to develop drug delivery system.

Objective To investigate the effect of Smad7 gene on the activation of rat hepatic stellate cells (HSCs) in primary culture and on the regulation on gene expression of HSCs. Methods HSCs were isolated from male SD rats by collagenase perfusion of liver from portal vein and by 8.2% Nycodenz gradient centrifugation, and thereby transfected with AdSmad7 and AdGFP (control) respectively. The mRNA expression of transforming growth factor (TGF) ?1, Smad3 and Smad7 were measured by RT-PCR. Meanwhile, Smad7 and ?-smooth muscle actin (?-SMA) expressions were detected by immuno- cytochemistry. Results The expression of Smad7 mRNA in AdSmad7 group increased remarkably compared with TGF?1 control group, while the expression of Smad3 and TGF?1 mRNA remains unchanged. The expression of Smad7 protein was significantly higher in AdSmad7 group than that in other three groups. Accordingly, the expression of ?-SMA protein in the group of HSCs transfected with AdSmad7 was the lowest(P

Objective: To explore the roles of CD4+CD25+and CD8+CD28- T regulatory cells in spontaneous tolerance of inbred rats orthotopic liver Transplantation.Methods: The orthotopic liver transplantation models of spontaneous tolerance were established between BN (donor) and LEW (recipient) inbred rats whose survival time was more than 120 days, The expression of CD8+CD28- T and CD4+CD25+T cells in PBMC and spleen were determined by flow cytometry. CD8+CD28- T and CD4+CD25+T cells were sorted by immunomagnetic beads. The expressions of CD25, CD28, CD152 and CD45RO on CD8+CD28- T and CD4+CD25+T regulatory cells were also determined by flow cytometry. Results: Compared with control groups, the proportion of CD8+CD28-T regulatory cells in spontaneous models was significantly increased in peripheral blood mononuclear cells (PBMC) and spleen [PBMC:(30.2?5.8)% vs(16.4?6.1)%;Spleen(23.7?7.2)% vs(13.4?5.5)% P

Objective　To manufacture adriamycin-porous tricalcium phosphate (A-PTCP) ceramic drug delivery system (DDS)as a possible method for bone defect treatment after bone tumor operation. Methods　A-PTCP DDS was made from putting adriamycin into PTCP. Thirty rabbits were divided randomly into group A(24 rabbits) and group B(6 rabbits). A-PTCP was implanted in the greater trochanter of the right femur in group A. Adriamycin were injected into veins in group B. Muscle around A-PTCP and plasma were taken out at different period. Adriamycin concentrations in muscle and plasma were measured by high performance liquid chromatography (HPLC). Results　A-PTCP could gradually release adriamycin over 10 weeks. Adriamycin concentrations in the muscle were higher than that in plasma. Conclusion　A-PTCP may be a new method for repairing bone defects after bone tumor operation.