Objective To set up an animal model of high altitude pulmonary edema(HAPE) by putting SD rats in the condition simulating high altitude where is hypoxic and cold,in addition the SD rats are forced to swim.Methods SD rats were divided into normal control group and experiment group(SD rats were put into the stimulated 4 000-meter low pressure chamber;Temperature: 8:00-20:00 10 ℃,20:00-8:00 4 ℃;Everyday the rats were forced to swim 6 times a day,once every 4 hours,each for 40 min).The rat lung arterial pressure was observed and pulmonary tissue was collected for pathological examination at 24,48,72 h.Results PaO_(2),SaO_(2),_()PaCO_(2) in the experiment decreased significantly.Lung arterial pressure increased significantly at 72 h,consistent with the progression of clinical HAPE.About 70.2% rats in the experiment exhibited swollen and thicken pulmonary tissue,the smaller alveolar cavity,but no case of alveolar edema was found.Conclusion The animal model of HAPE is successfully replicated,but the alveolar model of HAPE needs further study.

In multilayer spiral CT heart and coronary artery imaging, image data are acquired by ECG gating technology when heart beats slowest so as to restrain pulsation pseudo-image. ECG gating technology includes foresight ECG gating and retrospective ECG gating. The principle, methods and mutual image reconstruction are introduced in this paper.

Objective To study the effects of hyperbaric oxygen therapy on traumatized cerebral neurons and investigate its mechanism in attenuating cerebral damage.Methods Seventy-two rats were randomly assigned to a control group,a traumatic brain injury group or a traumatic brain injury group treated with hyperbaric oxygen. Each group was observed 1,7 and 24 days after the operation.TUNEL was used to examine the distribution of apop- tosis cells.An immunohistoehemical method was used to examine the distribution of Bcl-2 and Bax immunoreactive- positive cells in the brain tissues.Results The average percentages of both apoptosis cells and Bcl-2 immunoreac- tive-positive cells were lower in the CAl region of the traumatized brains treated with hyperbaric oxygen than in those of the traumatic injury group at each time of observation.A significant difference in Bax immunoreactive-positive cells between the two groups was also observed.Conclusion Hyperbaric oxygen therapy can significantly protect neurons against traumatic brain injury and modulate the expression of the apoptosis related genes Bcl-2 and Bax.This may ex- plain the protective mechanisms of hyperbaric oxygen therapy in treating traumatic brain injury.

Objective To compare analgesic efficacy of intravenous postoperative and preemptive analgesia with flurbiprofen axetil combined with small dose of morphine and with morphine alone. Methods One hundred and twenty patients were randomly divided into four groups: group A,morphine 30 mg +0.9% NaCl for postoperative analgesia,n=30;group B,morphine 20 mg +0.9% NaCl for postoperative analgesia,n=30;group C,flurbiprofen axetil 100 mg + morphine 20 mg +0.9% NaCl for postoperative analgesia,n=30;group D,flurbiprofen axetil 100 mg before operation + morphine 20 mg +0.9% NaCl for postoperative analgesia,n=30.The drugs in each group were diluted to 100 mL and infused by a pump at a rate of 2 mL/h with a patient-controlled analgesia(PCA)bolus of 2 mL after a loading dose of 5 mL.The visual analogue scale(VAS),demanding times for PCA and incidence of side effects were recorded during the period of postoperative 24 h. Results The VAS of group B at 3 h after operation was significantly higher than those of the other three groups(P

Epidemiologic Studies ; Humans ; Neoplasms ; epidemiology

Objective To investigate the diagnostic value of serum soluble transferrin receptor(sTfR) in children with iron deficiency anemia(IDA).Methods Sixty-three children with microcytic hypochromic anemia were divided into IDA and non-IDA(n-IDA)groups,on which sTfR and other iron metabolism related indexes such as serum ferritin(SF) and serum iron(SI) were measured,and(t-test) between groups and analysis of ROC curve were carried out.Results The mean concentration of sTfR in the group of IDA was above normal value and t-test difference was extremely significant compared with n-IDA group(P

Hot melt extrusion (HME) is an effective method to make the drugs form amorphous solid dispersions (ASD) inmicrostructure, through which we can improve the dissolution behavior so as to improve the bioavailability ofhydrophobic drugs This review mainly focuses on the. screening of active pharmaceutical ingredients and polymer carriers, feasibility evaluation of the technique, and the control of key parameters of preparation process, summarizes the application of HME to develop drug delivery system.

Objective To investigate the effect of Smad7 gene on the activation of rat hepatic stellate cells (HSCs) in primary culture and on the regulation on gene expression of HSCs. Methods HSCs were isolated from male SD rats by collagenase perfusion of liver from portal vein and by 8.2% Nycodenz gradient centrifugation, and thereby transfected with AdSmad7 and AdGFP (control) respectively. The mRNA expression of transforming growth factor (TGF) ?1, Smad3 and Smad7 were measured by RT-PCR. Meanwhile, Smad7 and ?-smooth muscle actin (?-SMA) expressions were detected by immuno- cytochemistry. Results The expression of Smad7 mRNA in AdSmad7 group increased remarkably compared with TGF?1 control group, while the expression of Smad3 and TGF?1 mRNA remains unchanged. The expression of Smad7 protein was significantly higher in AdSmad7 group than that in other three groups. Accordingly, the expression of ?-SMA protein in the group of HSCs transfected with AdSmad7 was the lowest(P

Objective To study the value of echocardiogram in preoperative examination for the diagnosis of silent myocardial is-chemia in patients before surgery .Methods 172 patients with surgical preoperative suspected diagnosis of coronary heart disease , were divided into heart valve surgery group (72 cases) and non heart valve surgery group (100 cases) .All of the patients had echo-cardiography and coronary angiography before 2 weeks of operation .We drew a series of analysis to assess the sensitivity、specialty and accuracy of diagnosis of silent myocardial ischemia with regional wall motion abnormality (RWMA) and wall motion score in-dex (WMSI) .Results Coronary angiography as the gold standard of coronary heart disease diagnosis .The sensitivity of RWMA for the diagnosis of silent myocardial ischemia in patients undergoing heart valve surgery group and non heart valve surgery group was 85 .7% and 100 .0% ,while the specificity of RWMA was 100 .0% and 80 .0% ,respectively ,the differences between the two groups had no statistical significance (P>0 .05) .We also drew the ROC curve of WMSI for the diagnosis of silent myocardial ische-mia ,maximum area under the ROC curve of WMSI were 2 .20 and 2 .05 .In patients undergoing cardiac surgery and non-cardiac sur-gery ,the curves indicated that the sensitivity were 79 .7% and 75 .9% ,the specificity were 83 .0% and 67 .7% ,respectively .Area under the ROC curve was 0 .832 and 0 .702 ,but the difference was not statistically significant (P>0 .05) .Conclusion Using RW-MA and WMSI as reference indicators ,surgical preoperative echocardiography in the diagnosis of patients with silent myocardial is-chemia has good sensitivity ,specific ,and accurate ,there is no statistically significant difference between heart valve surgery and non heart valve surgery .

Objective To explore the relationship between calcium-sensing receptor's expression and papillary thyroid carcinoma.Methods Seventy cases of postoperative papillary thyroid carcinoma were selected.Immunohistochemical technique was used to detect expression of calcium-sensing receptor in papillary thyroid carcinoma,thyroid benign lesions,and normal thyroid tissue,respectively.SPSS 17.0 statistical analysis was used with non parametric test,P＜0.05 indicated significant difference.Results The expression of calcium-sensing receptor in papillary thyroid carcinoma,benign thyroid,and normal thyroid were significantly different (90％,40％,0％,respectively; P ＜ 0.05).The expression of the calcium-sensing receptor in the group of papillary thyroid carcinoma with calcification was significantly higher than that in thyroid cancer without calcification (P ＜ 0.05).Conclusions This study suggests that the calcium-sensing receptor may be associated with papillary thyroid carcinoma and may play a key role in the calcification of the carcinoma.