1.Long snake moxibustion for 38 cases of gout.
Tian-You HE ; Li ZHANG ; Hong-Yan SHAO
Chinese Acupuncture & Moxibustion 2014;34(6):605-606
Acupuncture Points
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Adult
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Aged
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Female
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Gout
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therapy
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Humans
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Male
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Middle Aged
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Moxibustion
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Treatment Outcome
2.Acupuncture at Yinlingquan (SP 9), Taichong (LR 3) mainly plus blood-letting therapy for 52 cases of gout.
Hong-Yan SHAO ; Tian-You HE ; Li ZHANG
Chinese Acupuncture & Moxibustion 2013;33(6):526-526
Acupuncture Therapy
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Adult
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Aged
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Bloodletting
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Female
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Gout
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therapy
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Humans
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Male
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Middle Aged
3.Optimization of Xylanase Production by Paecilomyces thermophila in Solid State Fermentation
Shao-Qing YANG ; Qiao-Juan YAN ; Zheng-Qiang JIANG ; Li-Te LI ; You-Zhi WANG ;
Microbiology 1992;0(03):-
A new thermophilic fungus J18 isolated from the soil samples was identified as Paecilomyces thermophila. This strain produced effectively xylanase utilizing several lignocellulosic materials in the solid-state fermentation (SSF) , and wheat straw was the best carbon source. The results of single-factor-experiment showed that the wheat straw of particle size 0. 3 mm ~ 0.45 mm, initial moisture content of 83% , initial pH of 7. 0 and cultivation temperature of 50℃were the optimal conditions for xylanase production. Under the optimized conditions, it produced 18 580 U/g dry substrate after 8 days of cultivation. Therefore, xylanase production by Paecilomyces thermophila in SSF possess great potential for commercial applications.
4.Evaluation Study on the Life Quality of Gastroesophageal Reflux Disease Patients
Shao-Neng LIU ; You LI ; Dong YAN ; Hongmei WU ; Jia LIU ; Huimin LIU ;
Chinese Journal of Information on Traditional Chinese Medicine 2006;0(11):-
Objective To evaluate the life quality of gastroesophageal reflux disease (GERD) patients. Methods Classify the definite GERD patients by differentiation of symptoms and signs first, and then evaluate their quality of life from physiological function, physiological role, body pain, general health, activity, social function, affection function and mental health with scale SF-36. At the same time, 100 healthy persons were brought into the study as control. Results The life quality of GERD patients decreased, and the body pain, general health, social function and mental health decreased more obviously. Difference also lies among the life quality of patients of different syndromes, and the scores of physiological function, physiological role, general health and mental health show significant differences among the four syndromes. Conclusion The life quality of GERD patients decreased obviously.
5.Construction and experimental study on off-line hybrid bioartificial liver supporting system with human liver cell line.
Yi-Hui RONG ; Hong-Ling LIU ; Shao-Li YOU ; Wan-Shu LIU ; Yan HU ; Jun HOU ; Pan-Yong MAO ; Shao-Jie XIN
Chinese Journal of Experimental and Clinical Virology 2010;24(3):193-195
OBJECTIVETo construct an off-line hybrid bioartificial liver supporting system with human liver cell line, and study it's effect on the plasma from patients with liver failure.
METHODSWe established the bioreactor using Psu-2s (Fresenius) cultured with Hep G2 cell transfected with human augmenter of liver regeneration (hALR) gene, then constructed a hybrid bioartificial liver supporting system, at last using the bioartificial liver support system to purify the plasma treated 2 hours with serum bilirubin absorbent, separated from acute on chronic liver failure patients infected by hepatitis B virus.
RESULTSBioreactor was successful constructed. The cell viability in perigastrum of bioreactor is 85.2% and cell propagated rapidly. Before and after treating with bilirubin absorbent, serum total bilirubin was (176.19 +/- 54.14) micromol/L and (50.1 +/- 16.85) micromol/L respectively (P = 0.0002). While there were no significance difference in the level of albumin, urea and glucose. At the begin and end of treatment with bioartificial liver, serum total bilirubin was (50.10 +/- 16.85) micromol/L and (30.27 +/- 15.02) micromol/L respectively (P = 0.000), the urea and albumin increased, urea has significantly difference, but the change of albumin hasn't.
CONCLUSIONThe off-line hybrid bioartificial liver supporting system with human liver cell line were builded successfully and have synthesis and metabolism functions for acute on chronic liver failure patients.
Adult ; Artifacts ; Bilirubin ; metabolism ; Bioreactors ; standards ; Chimera ; End Stage Liver Disease ; physiopathology ; Hepatocytes ; metabolism ; physiology ; Humans ; Liver ; physiology ; Liver Failure ; Liver, Artificial ; utilization ; Male ; Middle Aged
6.Brain arousal dysfunction in severe craniocerebral injury treated with acupuncture.
Xiao-Hua TU ; Zeng-Yi HE ; Xiao FU ; Yan-Hua CHEN ; You-Lin CHEN ; Shao-Jun KANG
Chinese Acupuncture & Moxibustion 2010;30(12):974-976
OBJECTIVETo explore the early rehabilitation effect of acupuncture on brain arousal in severe craniocerebral injury.
METHODSOne hundred and two cases of severe craniocerebral injury were randomly divided into an observation group and a control group, 51 cases in each one. Based on the conventional nursing care in neurological external medicine, in observation group, acupuncture was applied at Shuigou (GV 26), Neiguan (PC 6) and Sanyinjiao (SP 6) mainly. In control group, functional electric stimulation was applied at stimulate the affected muscles of the upper limbs. Thirty days later, the lucid rate from coma, lucid interval and clinical efficacy were compared between two groups. RESULTS; The lucid rate from coma was 82.4% (42/51) in observation group, which was higher than 56.9% (29/51) in control group (P < 0.01). The lucid interval in observation group was shortened remarkably as compared with control group (P < 0.01), and the clinical efficacy was superior apparently to that in control group (P < 0.01).
CONCLUSIONOn the basis of conventional treatment, acupuncture intervention at early stage can accelerate the recovery of brain arousal function in patients with severe craniocerebral injury.
Acupuncture Therapy ; Adolescent ; Adult ; Aged ; Arousal ; Brain ; physiopathology ; Child ; Craniocerebral Trauma ; physiopathology ; rehabilitation ; therapy ; Humans ; Male ; Middle Aged ; Young Adult
7.Effects of cofilin phosphorylation on the actin cytoskeleton reorganization induced by shear stress.
Yan-hui LIU ; You-rui LI ; Min-feng SHAO ; Xiao-juan ZHANG ; Qiang FU
Chinese Journal of Stomatology 2010;45(12):763-766
OBJECTIVETo explore the effects of cofilin on the actin cytoskeleton reorganization in osteoblasts induced by fluid shear stress.
METHODSFluid shear stress (1.2 Pa) was applied to osteoblasts for 0 (control group), 15, 30, 45, 60, 120 min in vitro. Cells were stained with fluorescein isothiocyanate (FITC)-phalloidin for fiber-actin, and confocal laser scanning microscope(CLSM) was used to observe the fluorescence of fiber-actin. Western blotting was used to detect the expression of the cofilin and the phospho-cofilin.
RESULTSActin filaments became organized into stress fibers that were thicker and more abundant than those in non-flowed cells. The fluorescence intensity (38.00 ± 6.88) of fiber-actin after 120 min (42.93 ± 6.41) loading it was 2.8 times as much as that in control group (15.41 ± 3.60, P < 0.05). Additionally, the level of phospho-cofilin protein was dramatically elevated after loading. Fluid shear stress induced an initial decrease of cofilin at 60 min. However, at 120 min cofilin (0.254 ± 0.026) increased to 1.5 times as much as that at 60 min (0.162 ± 0.004).
CONCLUSIONSThe results indicate that cofilin phosphorylation mediates fiber-actin reorganization in the osteoblasts induced by fluid shear stress.
Actin Cytoskeleton ; ultrastructure ; Actin Depolymerizing Factors ; biosynthesis ; Humans ; Osteoblasts ; ultrastructure ; Phosphorylation ; Stress, Mechanical
8.Clinical study of pulmonary infection in kidney transplantation recipients taking new immunosuppressant.
Yan-Xuan ZHANG ; Li-Xin YU ; Shao-Jie FU ; Jun-Sheng YE ; Xiao-You LIU
Journal of Southern Medical University 2008;28(6):1037-1040
OBJECTIVETo explore the etiopathogenesis, therapy and incidence of pulmonary infection in kidney transplantation recipients taking new immunosuppressant.
METHODSThe clinical data from 752 kidney transplant recipients were retrospectively analyzed, who were divided into 3 groups according to the immunosuppressants administered, namely group A (CsA+MMF+Pred, n=226), group B (FK506+MMF+Pred, n=386) and group C (FK506+Rap+Pred, n=140). The incidence and mortality of pulmonary infection were recorded and the analysis of etiopathogenesis, diagnosis and therapy of pulmonary infection were carried out in the 3 groups.
RESULTSFifty-three patients acquired post-transplant pulmonary infection. The incidence of pulmonary infection was 7.08% (16/226) in group A, 7.25% (28/386) in group B and 6.43% (9/140) in group C. One patient died in group A and 2 in group B. Among the 53 patients, 24 had simple bacterial infection, 9 had cytomegalovirus infection, 1 had mycotic infection, 17 had combined infection, and 2 had unidentified pathogen infection. Of the pathogenic bacteria detected, 68.35% were Gram-negative.
CONCLUSIONGram-negative bacteria are most likely responsible for pulmonary infection after kidney transplantation, which most possibly occurs within 6 months after kidney transplantation. Early diagnosis and early treatment are critical for decreasing the mortality of severe pneumonia and for improving the survival rate of the patients and grafts.
Adolescent ; Adult ; Aged ; Cyclosporine ; administration & dosage ; adverse effects ; Cytomegalovirus Infections ; chemically induced ; diagnosis ; therapy ; Female ; Gram-Positive Bacterial Infections ; chemically induced ; diagnosis ; therapy ; Humans ; Immunosuppressive Agents ; administration & dosage ; adverse effects ; Kidney Transplantation ; Lung Diseases ; chemically induced ; diagnosis ; therapy ; Male ; Middle Aged ; Tacrolimus ; administration & dosage ; adverse effects
9.Effects of over-expressing resistin on glucose and lipid metabolism in mice.
You LIU ; Qun WANG ; Ying-bin PAN ; Zhi-jie GAO ; Yan-fen LIU ; Shao-hong CHEN
Journal of Zhejiang University. Science. B 2008;9(1):44-50
Resistin, a newly discovered peptide hormone mainly secreted by adipose tissues, is present at high levels in serum of obese mice and may be a potential link between obesity and insulin resistance in rodents. However, some studies of rat and mouse models have associated insulin resistance and obesity with decreased resistin expression. In humans, no relationship between resistin level and insulin resistance or adiposity was observed. This suggests that additional studies are necessary to determine the specific role of resistin in the regulation of energy metabolism and adipogenesis. In the present study, we investigated the effect of resistin in vivo on glucose and lipid metabolism by over-expressing resistin in mice by intramuscular injection of a recombinant eukaryotic expression vector pcDNA3.1-Retn encoding porcine resistin gene. After injection, serum resistin and serum glucose (GLU) levels were significantly increased in the pcDNA3.1-Retn-treated mice; there was an obvious difference in total cholesterol (TC) level between the experiment and the control groups on Day 30. In pcDNA3.1-Retn-treated mice, both free fatty acid (FFA) and high density lipoprotein (HDL) cholesterol levels were markedly lower than those of control, whereas HDL cholesterol and triglyceride (TG) levels did not differ between the two groups. Furthermore, lipase activity was expressly lower on Day 20. Our data suggest that resistin over-expressed in mice might be responsible for insulin resistance and parameters related to glucose and lipid metabolism were changed accordingly.
Animals
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Blood Glucose
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analysis
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Cholesterol, HDL
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blood
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Cholesterol, LDL
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blood
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Fatty Acids, Nonesterified
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blood
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Glucose
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metabolism
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HeLa Cells
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Humans
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Lipid Metabolism
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Male
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Mice
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Resistin
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blood
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physiology
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Triglycerides
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blood
10.Predictive and prognostic value of monitoring lymphocyte subsets in peripheral blood before and after chemotherapy in patients with metastatic breast cancer
Bin SHAO ; Huiping LI ; Lijun DI ; Guohong SONG ; Hanfang JIANG ; Xu LIANG ; Chaoying WANG ; Ying YAN ; Xiaolin LIN ; Lina WANG ; Fengling WAN ; Yanhua YUAN ; Miaoning YOU
Journal of Peking University(Health Sciences) 2016;48(2):304-309
Objective:To detect the proportion of lymphocyte subsets in peripheral blood of the ad-vanced breast cancer patients before and after chemotherapy with docetaxel and thiotepa,as well as the association between the proportion of peripheral blood lymphocyte subsets with the response rate and prog-nosis.Methods:The proportions of lymphocyte subsets (CD3 +T cell,CD3 +/CD4 +T cell,CD3 +/CD8 +T cell,CD3 -/CD16 +56 +NK cell,CD3 +/CD16 +56 +T cell,CD19 +B cell,CD4 +/CD25 +T cell,CD8 +/CD28 -T cell,CD8 +/CD28 +T cell)in the peripheral blood of 86 patients were analyzed with flowcytometry before and after chemotherapy.The result was analyzed in combination with clinico-pathological data.Results:The proportion of regulatory T cells (Treg)after chemotherapy in the disease control patients decreased significantly compared with that of the progressive patients (P=0.034).The difference of the proportions of Treg before and after chemotherapy affected significantly the overall survi-val (OS).The OS of the patients with decreased proportion of Treg was significantly longer than that of the patients with increased proportion of Treg,which was 23.5 and 9.4 months respectively (P<0.05). Conclusion:The patients with decreased proportion of Treg after chemotherapy had higher response rate and better survival benefit.