Objective To explore the effect of celecoxib on neural function in rats with acute intracerebral hemorrhage.Methods SD rats were randomly divided into sham group,model group,inhibitor group and celecoxib group.Except the sham operation group,the cerebral hemorrhage model was made by autologous blood injection in each group.Rats in the inhibitor group were injected with extra cellular regulated protein kinase(ERK)inhibitor(DCZ19931)into the anterior ventricle on the basis of the model group.Celecoxib group rats were injected intraperitoneally with 100 mg·kg 1·d-1 celecoxib,for 2 weeks.The degree of neurological injury in rats was compared by Longa five-grade score.The water content of brain tissue of rats in each group was detected and the expression of aquaporin 4(AQP4)in brain tissue of rats in each group was detected by Western blotting.Enzyme-linked immunosorbent assay was used to detect the content of interleukin-1(IL-1β),tumor necrosis factor-α(TNF-α),superoxide dismutase(SOD)and malonaldehyde(MDA)in the brain tissue of rats in each group.Western blotting was used to detect the expression of mitogen-activated protein kinase(MAPK)/ERK signal pathway protein in rats of each group.Result Compared with sham group,the neurological function score,brain water content,relative expression level of AQP4 protein,content of IL-1β,TNF-α and MDA,relative expression levels of p-ERK1 protein,p-ERK2 protein and MAPK protein in model group were significantly increased,while content of SOD was significantly decreased(P＜0.05).Compared with model group,the neurological function score,brain water content,relative expression level of AQP4 protein,content of IL-1β,TNF-α and MDA,relative expression levels of p-ERK1 protein,p-ERK2 protein and MAPK protein in inhibitor group and celecoxib group were significantly decreased,while content of SOD was significantly increased(P＜0.05).Conclusion Celecoxib can effectively improve the neurological function of rats with acute intracerebral hemorrhage,and its mechanism may be related to the regulation of MAPK/ERK signal pathway and inhibition of inflammation.

OBJECTIVETo study the distribution of serotype and the positive rate of toxins among vibrio cholerae non-O(1) isolated from environmental waters in Foshan city.

METHODSWater specimens were collected from river and cultured for vibrio cholerae non-O(1). The PCR method was used to detect cholerae enterotoxin (CT) gene; the ELISA method was used to detect heat-stable toxin (ST) and heat-labile toxin (LT).

RESULTS478 vibrio cholerae non-O(1) strains were isolated from 1 644 water specimens, with a positive rate of 29.07%. Serological assay showed that the main serotype of vibrio cholerae non-O(1) in Foshan city is VBO(7). Positive rate of CT, ST and LT were 1.91%, 13.14% and 12.17%, respectively.

CONCLUSIONSA few non-O(1) strains were found to have several virulent factors simultaneously, and the results suggest that vibrio cholerae non-O(1) in environmental waters is potentially pathogenic and may affect people's health. It is necessary to pay attention to the prevention of diarrhoea caused by vibrio cholerae.

China ; Enterotoxins ; genetics ; Environmental Monitoring ; methods ; Enzyme-Linked Immunosorbent Assay ; Polymerase Chain Reaction ; Seasons ; Serotyping ; Vibrio cholerae non-O1 ; classification ; genetics ; isolation & purification ; Water ; analysis ; Water Microbiology