Mixed reality technology can achieve a 3D reconstruction of the patient's imaging data and organically combine the real scene. This allows to simulate a real medical operation in clinical teaching. Combined with large data analysis, mixed reality technology allows to build situational awareness data models, thus providing data support for teaching interaction and disease simulations. In the present work, the application of mixed reality technology and situational awareness in clinical teaching of cardiac macrovascular surgery is explored innovatively. These technologies open a new avenue for teaching, solving the issue that stymies traditional teaching approaches and improving the teaching efficiency. It is thus important to explore and promote the applications of this innovative teaching methodology particularly in clinical teaching.

Apoptosis ; drug effects ; Cell Line, Tumor ; Cell Proliferation ; drug effects ; Doxorubicin ; administration & dosage ; analogs & derivatives ; pharmacology ; Humans ; Precursor Cell Lymphoblastic Leukemia-Lymphoma ; drug therapy ; pathology ; Valproic Acid ; administration & dosage ; pharmacology

Humans ; Liver Diseases ; Practice Guidelines as Topic ; Prognosis

Objective To investigate the morphological changes in the sciatic nerve and the dorsal root ganglions (DRGs) and also gene expression in DRGs after non-freezing cold injury, and to explore the molecular mechanism of peripheral nerve cold injury and regeneration. Methods Twenty-four male Wistar rats were used. The sciatic nerve on one side was cooled to 4℃ for 2 h, and the sciatic nerve on the opposite side was exposed, but without cooling. Sciatic nerves and L4, L5 and L6 DRGs from both sides were harvested at the 1st, 2nd and 3rd week after cooling. Any pathological changes were observed using light and electron microscopy. Laser capture microdissection (LCM) was used to investigate the DRG neurons' gene expression. The array result was verified with RT-PCR for eight genes. Results Large fiber degeneration was obvious by the 7th day after cooling. Myelinated fiber regeneration had begun by the 14th day, so this time was chosen to explore the neurons' gene expression. Ninety-six genes and expressed sequence tags (ESTs) were up-regulated greater than 2 fold. Their proteins' functions were classified as adaptive response to external stimulus, apoptosis regulation, cell adhesion, immune and inflammation response,nerve regeneration, pain associated molecules, microtubule cytoskeleton, ion-channels, neurotransmitters and receptors, and neuropeptides. Conclusions A complex molecular mechanism is involved in cold injury and regeneration of the sciatic nerve, and many genes are involved. Large scale microarray analysis is a potent means to screen out related genes, thus suggesting future repair strategies.

Objective To evaluate the life quality of gastroesophageal reflux disease (GERD) patients. Methods Classify the definite GERD patients by differentiation of symptoms and signs first, and then evaluate their quality of life from physiological function, physiological role, body pain, general health, activity, social function, affection function and mental health with scale SF-36. At the same time, 100 healthy persons were brought into the study as control. Results The life quality of GERD patients decreased, and the body pain, general health, social function and mental health decreased more obviously. Difference also lies among the life quality of patients of different syndromes, and the scores of physiological function, physiological role, general health and mental health show significant differences among the four syndromes. Conclusion The life quality of GERD patients decreased obviously.

Objective To study the effects of lucentis preventing vitreous hemorrhage after vitrectomy for diabetic retinopathy combined with iris neovascularization.Methods The clinical data of 70 patients (70 eyes) underwent vitrectomy for diabetic retinopathy combined with iris neovascularization during 2009 to 2015 in our hospital were retrospectively analyzed.The control group (30 eyes) accepted panretinal photocoagulation (PRP),re-vitrectomy,cyclocryotherapy,and the study group (40 eyes) had the 0.5mg lucentis in addition.The follow-up time was 3 months,and the visual acuity,IOP,vitreous hemorrhage,INV regression and complication were observed.Results At 1 month,2 months in the follow-up,the visual acuity of study group was better than the control group,but there was no significant difference along with the follow up.The average preoperative IOP was (26.312 ±4.566) mmHg (1 kPa =7.5 mmHg) in the study group and (24.586 ±5.783) mmHg in the control group,and at the end of the follow up,IOP was (18.576 ±4.762) mmHg in the study group and (28.587 ±7.786) mmHg in the control group,there was statistical difference between the two groups (P =0.041).The intraoperative and postoperative anterior chamber or vitreous hemorrhage occurred in 15 cases,5 cases of the control group,and 3 cases,1 case of the control group,there were significant differences (all P =0.000).At the end of the follow up,3 eyes (7.5％) developed to NVG in the study group and 10 eyes (33.3％) in the control group,there was statistical difference (P ＜ 0.05).Conclusion Lucentis can effectively eliminate the new vessels,reduce the incidence of neovascularization glaucoma and vitreous hemorrhage for patients after vitrectomy for diabetic retinopathy combined with iris neovascularization,and improve the visual acuity in a short time.

This paper introduces the preamplifier for the microcomputer-detecting system of 3-Channel ECG signals and its technical specifications. The practical experiments and clinical applications show that its performances conforms with all the requirements of the system.
Amplifiers, Electronic ; Electrocardiography ; instrumentation ; Equipment Design ; Heart Diseases ; diagnosis ; Humans ; Image Enhancement ; instrumentation ; Microcomputers ; Signal Processing, Computer-Assisted

AIM: To discuss the tongxinluo supermicropowder's interventional effect on aorta endodermis of rabbits which are fed with fatty forage plants.METHODS: Thirty-two healthy male New Zealand rabbits were divided into 4 groups randomly: control group,model group,atorvastatin treatment group,and tongxinluo treatment group.The control group was fed with common feedstuff,and all the other groups were fed with fatty feedstuff.The atorvastatin treatment group and the tongxinluo treatment group were given suspension of atorvastatin(3 g?kg-1?d-1) and tongxinluo supermicropowder(0.31 g?kg-1?d-1) by intragastric administration on the basis of fatty feedstuff.All the groups were fed with medicine for 6 weeks.At the end of 6 weeks,blood lipid levels were observed by enzymic method,the levels of blood serum NO and MDA and the activity of SOD were observed by chromatometry,the expression of nuclear factor-kappaB(NF-?B),intercellular adhesion molecule-1(ICAM-1) was detected,and VCAM-1mRNA expression in the aortic tissue was examined by reverse transcription polymerase chain reaction(RT-PCR).RESULTS: The levels of blood serum TC,TG,LDL-C,HDL-C,and MDA were increased significantly compared with the control group(P

The object of this study is to preparate the berberine hydrochloride (BBH) resin compound with taste masking effect. We took the BBH as the model drug and Amberlite IRP69 as the drug carriers, uncovered the curve of solubility of BBH in different cosolvent with a certain range of temperature, and then used it to calculate the parameters during the preparation of the complex such as adding quantity of BBH and the reaction temperature. Afterwards, the characteristic and in vitro release experiments were studied to verify the formation and predict the in vivo release behavior of the complex. The results showed that in the condition of using 60% ethanol as a cosolvent and stirring at 50 ℃ for 1 h, the drug loading and drug availability of the complex are at about 35% and 64%, respectively, and has a better taste-masking effect. In this study, a method was provided for preparing a taste-masking preparation of BBH.

Objective To discover the influence of thiomersalate exposure on the behavior and humoral immunity of premature rats.Methods According to common thiomersalate dose of vaccine used in human,the surface conversation method was used to change the dose to 1,2,3,4 times for rats(32.8,65.6,98.4 and 131.2 μg/kg),and the thiomersalate was separately injected to the gluteus maximus of premature rats respectively,which were delivered on the 20th day of gestation by cesarean for the injection on day 1 (premature).Simultaneously,saline was injected to the control group.On day 44 to 48 after injection,the Morris water maze test was used to evaluate the spatial learning and memory abilities of rats and on the post-injection day 49,immunoglobulin content in rat blood and cerebrospinal fluid was measured.Results The spatial learning abilities of rats in 131.2 μg/kg group and the memory abilities in 65.6 μg/kg,98.4 μg/kg and 131.2 μg/kg group were significantly weaker than those in 9 g/L saline group(all P ＜0.001).The immunoglobulin content in blood and cerebrospinal fluid changed in different groups exposed to thiomersalate.The levels of IgG,IgA,IgE,IgM in 98.4 μg/kg group and 131.2 μg/kg group rose significantly higher than those in 9 g/L saline group(all P ＜ 0.01).Analysis of rectilinear correlation between the immunoglobulin and learning abilities of rats revealed that IgG,IgA,IgE levels in the blood were correlated with the spatial learning abilities (r =0.36,0.47,0.50,all P ＜ 0.05) and the levels of IgG,IgA,IgE,IgM in blood were rectilinear correlated with the memory abilities of rats (r =-0.39,-0.43,-0.49,-0.38,all P ＜ 0.05).In rats'cerebrospinal fluid,IgG,IgA,IgE,IgM levels were rectilinear correlative with both the spatial learning and memory abilities (r =0.48,0.59,0.54,0.41,all P＜0.05;r=-0.39,-0.61,-0.57,-0.44,all P＜0.05).Conclusion With the dose ofthiomersalate increased,the learning and memory and humoral immunity are sustained damaged for premature rats.