1.Epidemiologic study on symptomatic gastroesophageal reflux disease and its risk factors in outpatient populations of internal medicine in Zhejiang province
Hong ZHANG ; Jing XU ; You-Ruing LI ;
Chinese Journal of Digestion 2001;0(07):-
Objective To investigate the prevalence of gastroesophageal reflux disease(GERD)in outpatients who visited department of internal medicine in hospitals of Zhejiang province and its risk fac- tors were analyzed.Methods The epidemiologic study was carried out on outpatients with reflex diagnos- tic questionnaire(RDQ).The data was analyzed with Logistic regression methods.Results The preva- lence of GERD in those patients was 7.28%(95% CI:6.87%-7.69%)in preliminary screening.There was significant correlation among gender,age,degree of labour intensity,status of marriage and the oc currence of GERD(P
2.Long snake moxibustion for 38 cases of gout.
Tian-You HE ; Li ZHANG ; Hong-Yan SHAO
Chinese Acupuncture & Moxibustion 2014;34(6):605-606
Acupuncture Points
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Adult
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Aged
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Female
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Gout
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therapy
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Humans
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Male
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Middle Aged
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Moxibustion
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Treatment Outcome
3.Fifty cases of dyspnea treated by warming needle moxibustion.
Ren-Ding WU ; You-Hong LI ; Jing-Min SONG
Chinese Acupuncture & Moxibustion 2012;32(9):856-857
Acupuncture Therapy
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instrumentation
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Adolescent
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Adult
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Aged
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Aged, 80 and over
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Combined Modality Therapy
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Dyspnea
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therapy
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Female
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Humans
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Male
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Middle Aged
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Moxibustion
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Young Adult
4.Acupuncture at Yinlingquan (SP 9), Taichong (LR 3) mainly plus blood-letting therapy for 52 cases of gout.
Hong-Yan SHAO ; Tian-You HE ; Li ZHANG
Chinese Acupuncture & Moxibustion 2013;33(6):526-526
Acupuncture Therapy
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Adult
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Aged
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Bloodletting
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Female
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Gout
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therapy
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Humans
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Male
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Middle Aged
5.Auricular points injection for 76 cases of chloasma.
You-hong REN ; Bing-quan MA ; Jie LI
Chinese Acupuncture & Moxibustion 2014;34(11):1122-1122
6.The expression and significance of human macrophage metalloelastase in both gastric cancer cell lines and gastric cancer tissues
Hong ZHANG ; You-Ming LI ; Chun-Xiao CHEN ;
Chinese Journal of Digestion 1996;0(05):-
Objective To investigate the expression of human macrophage metalloelastase(HME) both in gastric cancer cell lines and gastric cancer tissues,and to find the role of HME in gastric carcino genesis.Methods Fifty eight patients who were operated in our hospital during April to Aug.2003 were enrolled.The samples taken from cancer,paracancer or normal tissues of these patients and cancer cell lines(MGC-803,SGC-7901,AGS)were detected for HME protein and HME mRNA expressions by Western blot and immunohistochemistry,RT-PCR and fluorescence quantitative PCR,respectively. Results Both HME mRNA and HME protein expressions were found in all three gastric cancer cell lines.The expressions of HME mRNA and HME protein in gastric cancer tissues was increased signifi- cantly compared with that in normal tissues(P0.05).Conclusions The increased HME expression in gastric cancer tissures compared with normal tissue indicate that HME may be a potential tumor marker for gastric cancer.
7.Dynamic observation on IgG and its subclasses and IgE in sera of mice by immunization with mixed recombinant of BCG-Em Ⅱ/3 and BCG-Em14-3-3 vaccine of Echinococcus multilocularis
Wen-gui, LI ; Hong, WANG ; You-ming, ZHU ; Mei, YANG
Chinese Journal of Endemiology 2009;28(3):280-282
Objective To dynamically observe changes of IgG, its subclasses and IgE in sera of mice by immunization with mixed recombinant of BCG-Em Ⅱ/3 and BCG-Em14-3-3 vaccine of Echinococcus multilocularis (Era). Methods Forty Balb/c mice of 12-14 week old and 20-25 g weight were intranasally vaccinated by the vaccine, 4 mice were killed randomly by the weight on 0,2,4,6,8,10,12,14,16 and 18 weeks of immunization respectively, sera were gathered from the eyeball to measure IgG, its subclasses and IgE by routine ELISA. Results Levels of IgG, IgG2a and IgG2b in the sera of mice increased obviously on 2-18 weeks, reached the highest level on 10, 4 and 4 weeks respectively, the value was 0.095±0.033,0.022±0.001,0.023±0.003 respectively, as compared with the value on 0 week(0.030±0.013,0.012±0.004,0.013±0.004), the difference being statistically significant(q=2.95,4.87,2.81 respectively, P < 0.01 or P < 0.05); levels of IgG1, IgG3 and IgE in the sera of mice decreased remarkably on 2-18 weeks,came to the lowest level on 4,2,6 weeks respectively, the value was 0.031±0.004,0.136±0.002,0.114±0.002 respectively, as compared with the value on 0 week(0.192±0.007, 0.175±0.013,0.024±0.003), the difference being statistically significant (q =5.16,4.93,5.32 respectively, P < 0.01 or P < 0.05). Conclusion Helper T cell(TH) Ⅰ response is induced in mice by mixed recombinant of BCG-Em Ⅱ/3 and BCG-Em14-3-3 vaccine on early immunization.
8.Dynamic observation on subsets of splenocytes in mice by immunization with recombinant BCG-Eg95 vaccine of Echinococcus granulosus
Wen-gui, LI ; You-ming, ZHU ; Hong, WANG
Chinese Journal of Endemiology 2009;28(1):54-57
Objective To dynamically observe the changes of subsets of splenocytes in mice by immunization with recombinant BCG-Eg95 vaccine of Echinococcus granulosus(Eg).Methods Balb/c mice were divided randomly into 3 groups according to their weishts:intranasal group.per os group and PBS control.The mice were vaccinated intranasally or orally by the vaccine respectively in experimental groups.and the control mice were given phosphate buffer saline intranasally.These mice were killed to get spleen on 0,2,4,6,8,10,12, 14,16 and 18 week of immunization,respectively.Splenocytes were separated to measure subsets of CD4+ and CD8+T ceUs by FACsort.Results There were marked differences in ratio of CD4+ and CD8+ subsets among the different groups(F value were 21.56 and 22.08 respectively,P<0.05).There were very marked differences in ratio of CD4+ and CD8+ subsets in different weeks(F value were 5.75 and 6.29 respectively.P<0.01).In the intranasal group,CD4+ and CD8+ T subsets increased obviously in 6~18 weeks and 12 weeks,and reached the highest level on 10 and 12 week,espectively.Their values were 0.348±0.013 and 0.090±0.003.respectively.There were marked or very marked differences(q value were 7.32 and 5.32 respectively,P<0.01 or<0.05)in comparison with 0 week(0.230±0.022 and 0.069±0.015).In the oral group,CD4+and CD8+ subsets rose reinarkablv on 6-16 weeks and 8-18 weeks,achieved the hishest level on 10 and 16 weeks,respectively.Their vahes were 0.405± 0.006 and 0.096±0.004,respectively.There were marked or very marked difference(q value were 7.53 and 5.35 respectively,P<0.01 or<0.05)in comparison with week 0(0.230±0.022 and 0.069±0.015).Conclusion CD4+and CD8+T subsets may play an important role in immune response induced in mice by rBCG-Eg95 vaccine.
9.Study on the changes of cytokines of splenocytes in mice by immunization with recombinant BCG-Em Ⅱ/3 vaccine against Echinococcus multilocularis
Wen-gui, LI ; Hong NG WA ; You-ming, ZHU
Chinese Journal of Endemiology 2008;27(3):276-279
Objective To investigate the changes of cytokines of splenocytes in mice immunized with recombinant BCG-Em Ⅱ/3 vaccine of Echinococcus multilocularis(Em)and consequently challenged with Em protoscoleces.Methods Balb/c mice were subcutaneously or intranasally vaccinated and challenged with Em were separated and cultured with EmAg,ConA or PHA,respectively.The supematants were gathered to measure the levels of IL-2,IFN-γ,TNF-α.and IL-4 by ELISA Kits.Results The levels of IL-2,IFN-γ,TNF-α and IL-4 in the subcutaneous group were(34.6±2.7),(34.5±2.8),(265.0 ±0.0)and(9.8±2.6)ng/L respeetively:those in the intranasal group were(32.5±2.2),(33.6±2.7),(130.0±0.0)and(10.4±27)ng/L respectively;those in the control were(25.0±1.9),(30.0±0.0),(10.0±0.0)and(12.5±2.7)ng/L,respectively:there were statistical differences between the immunized groups and control group(P<0.01 or<0.05);The level of TNF-α in the subcutaneous group was higher than that in the intranasal group.Conclusion Th1 response has been induced in mice vaccinated with rBCG-Em Ⅱ/3 vaccine and challenged with Em protoscoleces.
10.Recent advances in the structure-activity relationship study of small-molecule sodium channel blockers with analgesic effects.
Wen LI ; You ZHOU ; Hong-Min LIU ; Qi-Dong YOU
Acta Pharmaceutica Sinica 2009;44(2):101-108
Pain is one of the common clinical symptom, previous studies have implicated sodium channels as a key constituent in pain signaling. Sodium channel blockers with efficient sodium channel blockade effect play an important role in analgesic treatment. However, most drugs used in clinic have many drawbacks and can not meet the demand of the clinical use. Therefore, for the development of new generation of sodium channel blockers, it is of great significance to find small molecule sodium channel blocking lead compounds with novel chemical scaffolds and new structures, sodium channel blocking activity and structure-activity relationship are discussed in detail, and current problems and trends in future research are also emphasized.
Analgesics
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chemistry
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pharmacology
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therapeutic use
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Animals
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Drug Design
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Humans
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Molecular Structure
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Neuralgia
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drug therapy
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Pain
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drug therapy
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Pain Measurement
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Sodium Channel Blockers
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chemistry
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pharmacology
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therapeutic use
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Sodium Channels
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drug effects
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Structure-Activity Relationship