According to the goal of promoting all-round ability and the core idea of PRICE,the Medical School of Shanghai Jiao Tong University improves the curriculum,teaching method,experimental teaching,multidimensional examination to explore the new system of teaching reform for eight-year clinical medical education.

Autophagy ; Child ; Humans ; Kidney Diseases

Adaptor Proteins, Signal Transducing ; chemistry ; metabolism ; Animals ; Apoptosis ; Cell Differentiation ; Cell Line, Tumor ; Cell Proliferation ; Humans ; Neoplasms ; pathology ; Phosphorylation ; Protein Isoforms ; RNA-Binding Proteins ; chemistry ; metabolism ; Transcription Factors ; antagonists & inhibitors

Carcinoma ; Cytological Techniques ; Humans ; Nasopharyngeal Neoplasms ; pathology ; Neoplastic Stem Cells ; cytology ; Oncogenes

With the development of magnetic resonance (MR) technology, the concern is focused on molecular and physiological imaging besides structural information. Ultra high field MR scanner shows great superiority. 3. 0-T MR scanners provide almost two-fold signal-to-noise ratio (SNR) compared with a standard 1. 5-T MR scanner. The higher baseline SNR can be converted into a variety of alternative benefits. There are still some other changes brought about by 3. 0-T, such as higher sensitivity to motion, susceptibility effect, more difference in resonant frequency among different metabolites, and so on. These features may be a double-edged sword in structural and functional imaging. But with the ceaseless progress of the technology and gains in experience, 3. 0-T systems will provide more information and show more advantages.
Animals ; Humans ; Image Enhancement ; methods ; Magnetic Resonance Imaging ; instrumentation ; methods ; Magnetic Resonance Spectroscopy ; instrumentation ; Sensitivity and Specificity

Objective To investigate the diagnostic value of serum soluble transferrin receptor(sTfR) in children with iron deficiency anemia(IDA).Methods Sixty-three children with microcytic hypochromic anemia were divided into IDA and non-IDA(n-IDA)groups,on which sTfR and other iron metabolism related indexes such as serum ferritin(SF) and serum iron(SI) were measured,and(t-test) between groups and analysis of ROC curve were carried out.Results The mean concentration of sTfR in the group of IDA was above normal value and t-test difference was extremely significant compared with n-IDA group(P

OBJECTIVE To investigate whether α-lipoic acid(α-LA)can promote the anti-prolifera-tion effect of pyrrolidine dithiocarbamic acid(PDTC)in Hep-2 cells. METHODS The laryngeal carcino-ma Hep-2 cells were cultured with α-LA,PDTC or α-LA+PDTC respectively for 12,24 and 48 h. The proliferation of Hep-2 cells was detected by WST-1 assay and soft agar colony formation while apoptosis and cell cycle were analyzed by Hoechst33258 staining and flow cytometry. RESULTS α-LA 5 -500 μmol·L-1 could not inhibit Hep-2 cell proliferation,but PDTC 5 -50 μmol·L-1 could( P ﹤0.05,P ﹤0.01). The cell proliferation inhibitory rate of PDTC 5 μmol·L-1 combined with α-LA 5,10 and 20 μmol·L-1 groups was much higher than in control group(P﹤0.01)and PDTC alone group(P﹤0.05). When α-LA 5 μmol·L-1 was used in combination with PDTC 5 μmol·L-1 for 12-48 h,the cell proliferation was inhibited in a time-dependent manner(r=0.987,P﹤0.05). When the cells were treated for 24 h,the number of soft agar colony formations in combined group was significantly smaller than that of both α-LA alone group(P﹤0.01)and PDTC alone group(P﹤0.05). The result of Hoechst33258 staining and flow cytometry indicated that after combined treatment with PDTC 5 μmol·L-1 and α-LA 5 μmol·L-1 for 24 h,the level of apoptosis and the percentage of cells in G2 / M stage were significantly increased compared with PDTC alone or α-LA alone treatment. CONCLUSION α-LA can enhance the anti-proliferation and pro-apoptosis effect of PDTC in Hep-2 cells.

Objective To explore the cause and treatment of chronic pain after tension-free repair of inguinal hernia.MethodsThe clinical data of 426 cases with inguinal hernia underwent the tension-free hernioplasty during February 2002 to September 2007 were retrospectively analyzed.ResultsTension-free hernioplasty was performed to all patients.According to operative methods,they were divided into two groups:polypropylene filling group(n=210)and expanded polytetrafluoroethylene(e-PTFE)mycromesh group(n=216).The chronic pain rate after operation,polypropylene filling group(9.0%,19/210)was significantly higher than e-PTFE mycromesh group(4.2%,9/216),P

Objective To review the clinical operation methods of abdominal incisional hernia. Methods Classification, operation method and fellow-up of 78 patients with abdominal incisional hernia were retrospectively analyzed. Results The average time of fellow-up was 26 months. Nineteen cases were repaired with simple suture with 3 cases (15.8%) recurrence, 57 cases were repaired with man-made material with 2 case (3.4%) recurrence. Conclusions Individual operation method should be chosen according to body condition, classification of the size of abdominal loss and abdominal hypertension. It is an effective method to repair the hernia of abdominal incision with man-made material.

Objective To introduce the clinical manifestations of REM sleep behavior disorder (RBD) which hasn't been reported in China ever before and to discuss the possible causes and mechanisms of the disease. Methods Ten cases of RBD verifted by polysomnography(PSG)were analyzed retrospectively. Results All patients were senile or in middle ages and most of them were male.The main clinical features included violent movements,shouting and other abnormal behaviors occuring in dreams when they fell asleep.Four cases were diagnosed as Parkinsonism,2 as multiple system atrophy and 1 as cerebral trauma.The causes of another 3 cases were unknown.all symptoms of them were relieved by using clonazepam administered prior to sleep.The PSG of all cases showed a loss of normal muscular atonia and an appearance of EMG activities during REM sleep. Conclusions RBD should be considered when patients showed abnormal especially violent behaviors in sleep.PSG should be essential for its correct diagnosis and the differentiation from partial complex epilepsy.Clonazepam given prior to sleep should be effective for treatment.