Objective To investigate the type A behavior, brain-derived neurotrophic factor (BDNF) and hypersensitive C-reactive protein (hs-CRP) levels in serum in patients with post stroke depression (PSD) ,and explore the correlation between them.Methods The HAMD-17 was used to assess the severity of depressive symptoms in patients with stroke (n=116).TABP,Social Support Scale, MMSE, NIHSS,mRS and BI were measured in PSD group(n=36) and non PSD group(NPSD group, n=80);fast blooding samples were analyzed with BDNF and hs-CRP in patients with PSD group, NPSD group and healthy controls (NC group).Results (1) The incidence of acute PSD was 31.03％.(2)The constituent ratio of type A behavior(83.33％ vs 57.50％), CH scores, TH +CH scores were significantly higher in PSD group than NPSD group(P＜0.01).Social Support Scale scores,MMSE scores were significantly lower in PSD group than NPSD group(P＜0.01, P＜0.05).NIHSS scores,mRS scores were significantly higher in PSD group than NPSD group, BI scores were significantly lower(P＜0.01).(3) The levels of serum BDNF,hs-CRP in the PSD group,the NPSD group and the normal control group were respectively (BDNF (617.24(274.98,976.22)) pg/ml, (938.19(615.88,1 714.76)) pg/ml, (4 149.46(1 658.83,7 583.34)) pg/ml);(hs-CRP(6.47(2.66,11.21)) mg/L, 1.77(0.74,6.51) mg/L, (0.86(0.47,1.59)) mg/L).The BDNF levels of PSD group were significantly lower and hs-CRP levels were significantly higher than NPSD group (P＜0.01).(4) The severity of depression was positively correlated with CH,TH+CH scores(r=0.38, P＜0.05;r=0.43, P＜0.01).CH scores were negatively correlated with social support(r=-0.38, P＜0.05),and were positively correlated with hs-CRP levels(r=0.34, P＜0.05).Conclusion In patients with post stroke depression,the BDNF levels decrease and hs-CRP levels increase,but severity of depression and cognitive function are not correlated with BDNF levels.Hostility and competition(CH) are correlated with the increasing hs-CRP levels.Patients with type A behavior, especially high CH scores, have immune system dysfunction and lower social support.Hostility and excessive competition are psychosocial risk factors occurring PSD.

Objective: To establish a HPLC for determination of paracetamol and vitamin C in Wei C Yinqao Tablets. Methods: A Hypersil C 18 column(4.6?250mm,5?m) was used with a mobile phase of 0.02mol/L potassium dihydrogen phosphate acetonitrile (90∶10). The detection wavelength was 249nm. Results: The average recoveries of paracetamol and vitamin C were 100.5%(RSD was 1.33) and 98.7%(RSD was 1.68%), respectively. Conclusion: The method is simple, rapid and accurate. It can be used for quantitative analysis of Wei C Yinqiao Tablets.

Objective To compare the effect of the different operation for mammary duct fistula (MDF). Method The clinical data of 85 cases of MDF were analysed retrospectively. Continuous section examinations between the multiple fistula was performed in 3 cases undergoing breastectomy.Results Among the 85 cases with 146 MDF, the opening located within 5cm around areola in 129 cases. MDF incision was performed on 38 cases, 34 cases cured, 4 cases recurred;MDF resection was performed in 44 cases, all the 44 cases were cured;simple mastectomy was performed on 3 cases of extensive breast inflammation,all the 3 cases cured. Conclusions Most of MDF occurs in main mammary duct. Most of the opening of MDF are around the areola. MDF incision or resection is the first choice of operation.

To detect the expression of the human papilloma virus (HPV) 16 and HPV18 in esophageal squamous cell carcinoma in Minnan area. Methods: Real-time fluorescence quantitative polymerase chain reaction (FQ-PCR) was applied to detect HPV DNA in 100 esophageal carcinoma sample and 100 normal tissues beside the tumor. Results:The positive rates of high-risk HPV 16 and HPV 18 infection in Minnan esophageal carcinoma and in normal tissues beside the tumor were 14.00%, 15.00%and 7.00%, 8.00%, respectively. The positive rates of lymph node and non-lymph node metastases were 40.98%and 10.25%, respectively. These re-sults exhibited statistical significance (P<0.01). HPV 16 and HPV 18 infection was uncorrelated with patient age, pathological type, and tumor grade (P>0.05). Conclusion:HPV16 and HPV 18 infection was correlated to esophageal squamous cell carcinoma in Minnan ar-eas. Such infection may also contribute to the occurrence and development of esophageal squamous cell carcinoma.

To investigate the expression of ICAM 1 and TNF ? in gastric mucosal cell during drug induced mucosal injury, the authors carried out the study in SD rats with aspirin by mouth with immunohistochemical method . The results showed that ICAM 1 began to express at 30min after administration of aspirin, peaked at 1h, maintained until 6h, decreased at 12h,and expressed slightly at 24h . TNF ? began to express at 15min, peaked at 3h to 6h,decreased at 12h, and expressed significantly at 24h. There was a slight ICAM 1 and TNF ? expression in gastric mucosal cells in the control group. It was suggested that the expression of ICAM 1 and TNF ? plays an important role in the mechanisms of gastric mucosal injury .

Objective To explore the principle and methods of normative minimally invasive surgery treatment on hypertensive intracerebral hemorrhage. Methods 49 cases of hypertensive intracerebral hemorrhage were treated by microsurgery with small incision. Results 46 cases survived and 3 cases died after surgery. 42 cases were followed up. Adopting GOS, 19 cases were normal, 12 cases were disable and could care for self, 5 cases were severely disable, sober and could not care for self, 3 cases were vegetable survival, and 3 cases were dead. Conclusion Application of minimally invasive surgery idea and normative treatment according to the patients' condition can obviously improve the efficacy of patients with hypertensive intracerebral hemorrhage.

Objective To explore the cause and treatment of chronic pain after tension-free repair of inguinal hernia.MethodsThe clinical data of 426 cases with inguinal hernia underwent the tension-free hernioplasty during February 2002 to September 2007 were retrospectively analyzed.ResultsTension-free hernioplasty was performed to all patients.According to operative methods,they were divided into two groups:polypropylene filling group(n=210)and expanded polytetrafluoroethylene(e-PTFE)mycromesh group(n=216).The chronic pain rate after operation,polypropylene filling group(9.0%,19/210)was significantly higher than e-PTFE mycromesh group(4.2%,9/216),P

Objective To review the clinical operation methods of abdominal incisional hernia. Methods Classification, operation method and fellow-up of 78 patients with abdominal incisional hernia were retrospectively analyzed. Results The average time of fellow-up was 26 months. Nineteen cases were repaired with simple suture with 3 cases (15.8%) recurrence, 57 cases were repaired with man-made material with 2 case (3.4%) recurrence. Conclusions Individual operation method should be chosen according to body condition, classification of the size of abdominal loss and abdominal hypertension. It is an effective method to repair the hernia of abdominal incision with man-made material.

Objective:To investigate the effect of heat shock protein 60(HSP60) on the functions of dendritic cells of ApoE-/-mice (mDCs) in vitro by signal transduction of mitogen-activated protein kinase (MAPK) family mediated by Toll-like receptors (TLRs).Methods:Myeloid mDCs were extracted in vitro from ApoE-/-mouse marrow,which were incubated as atherosclerotic animal model,and divided into three groups:HSP group,LPS group and control group.The three groups of mDCs were incubated to mature with mHSP60,LPS and normal saline,respectively and then examined the expression of TLR protein and mRNA by flow cytometry and real-time RT-PCR.In addition,the expression level of CD80 was examined by flow cytometry.The expression of MAPK family proteins was determined by Western blot analysis.The levels of cytokines secreted by mDCs,such as IL-6 and TNF-?,were analyzed by ELISA.Results:The expression of TLRs and MAPK families (p38 and ERK) was increased in the samples of HSP group,when compared with control group (P0.05).Conclusion:HSP60 is involved in activation of mDCs of atherosclerotic animal model by means of the signal transduction of MAPK family mediated by TLRs.

Objective To design and develop a quadrature demodulation circuit for detection of ampli-tude and phase information in EIT measurement. Methods A model structure file was built in matlab/simulink using the elements in Ahera DSP Builder and verified by simulated stimulation. Then the model file was convert-ed to VHDL file via Signal Compiler, and the synthesis, adaptation, simulation and hardware implementation were accomplished. Results The simulation and testing results show that the circuit can extract the real and imagi-nary information of the measured signal and it also meets the EIT measuring requirement, with acceptable mea-sure error. Conclusion The demodulation circuit based on DSP Builder can precisely give real and imaginary information of EIT and provides technological support for full-information complex impedance measurement.