Objective To study the expression of small breast epithelial mucin(SBEM) in the peripheral blood of the patients with breast cancer,and explore the correlation between micrometastasis and SBEM.Methods Peripheral blood samples were obtained from 92 patients with breast carcinoma,20 patients with benign breast diseases,10 patients with malignant tumor not originating from breast,and 10 normal healthy volunteers.SBEM-mRNA expression was detected by nested reverse transcription-polymerase chain reaction(Nested-RT PCR).Results Among 92 breast carcinoma patients,43(46.7%) were Nested-RT-PCR positive for SBEM-mRNA,but it was negative in all the other groups.The expression of SBEM-mRNA in peripheral blood did not correlate with ER or PR status,menstruation state or primary tumour size.but correlated with patient′s age,the state of Her-2,CEA,CA15-3 and axillary lymph node metastasis of breast cancer.Conclusions The expression of SBEM-mRNA in peripheral blood of breast cancer can be of potential use to monitor the micrometastasis of breast cancer.

BACKGROUND:Many patients underwent aspirin anticoagulation in preventing cardiovascular disease before hip and knee replacement. No report addressed the effect of aspirin on blood loss during perioperative stage in patients receiving hip and knee replacement. OBJECTIVE:To observe the effect of perioperative continuous low-dose aspirin anticoagulation application on total hip arthroplasty or total knee arthroplasty. METHODS: A total of 40 patients with primary total hip arthroplasty or total knee arthroplasty were enroled in the Department of Orthopedics, Tianjin First Center Hospital from December 2012 to August 2013. According to anticoagulation scheme, they were divided into two groups (n=20). In the observation group, 100 mg/d aspirin anticoagulation was continuously used before replacement for a long period, and the medicine was not withdrawn except the day of replacement. In the control group, aspirin anticoagulation was not used, and their ages were similar to the observation group. The operation was conducted by the same physician in the observation and control groups. 10 patients received total hip arthroplasty and 10 patients received total knee arthroplasty in both groups. Bleeding was stopped actively during replacement. After replacement, pressurized ice compress was used to reduce bleeding. At 48 hours after replacement, wound drainage, perioperative hemoglobin and the incidence of complications were recorded. Joint function recovery was observed at 3 months of folow-up. RESULTS AND CONCLUSION:Hemoglobin levels before and after operation, postoperative blood drainage at 48 hours, perioperative hemoglobin changes, the incidence of complication, and joint function score at 3-month folow-up did not show significant difference between both groups. These findings indicate that it is safe to use some measures for reducing blood loss and continue low-dose aspirin in the perioperative period. The use of aspirin did not impact blood loss.

Asian Continental Ancestry Group ; China ; epidemiology ; Fatty Liver ; epidemiology ; Humans ; Hyperlipidemias ; epidemiology

Acupuncture Therapy ; Adult ; Bloodletting ; Breast Diseases ; therapy ; Combined Modality Therapy ; Female ; Humans ; Hyperplasia ; therapy ; Mammary Glands, Human ; physiopathology ; Middle Aged ; Young Adult

Objective To discuss the effect of Yifei Mixture (YFM) on quality of life in patients with chronic obstructive pulmonary disease (COPD) at stable phase. Methods Sixty patients with COPD were randomly divided into the control group with small dose Aminophylline Sustained-release Tablets and inhaling Salbutamol or becotide (30 cases), and the observation group with YFM 100 mL, po, Bid (30 cases). Results There was significant statistical differences between the two groups, which shows that the observation group was superior to the control group in amelioration of syndrome, pulmonary function, the times of hospitalization yearlong days, single hospitalization and the integration of SGRQ. Conclusion YFM can slow down the progression of COPD, reduce the time of hospitalization and improve quality of life.

Recent studies show that the abnormalities of dickkopf-1 (DKK-1)are closely related to the bone metastasis and therapeutic effect of breast cancer,prostatic carcinoma,multiple myeloma,neuroblastoma and non-small cell lung cancer.Clinical researches of large sample on the relationships between serum DKK-1 concentration and malignant tumors bone metastases have important clinical significance for evaluating the value of DKK-1 as a clinical biological biomarker of bone metastases and understanding the tumor mechanisms of bone metastases.

Objective To discuss the clinical value of transesophageal echocardiography used in extracorporeal circulationcardiac valve replacement. Methods 89 patients received extracorporeal circulation cardiac valve replacement in our hospital from January 2012 to December 2014 were included in the study. Transesophageal echocardiography were performed preoperatively, intraoperation and postoperatively and the findings were compared to the result of preoperation transthoracic echocardiography exam. The measurement of aortic annulus diameter and detection rates of calcification obtained by transthoracic and transesophaged echocardiography were compared. Changes in heart function parameters before and after operation were compared. The alternations in surgery plan made after pre-operative transesophageal echocardiography exam and any relevant treatment or procedure performed according to intra-operation transesophageal echo findings were recorded. All the patients were followed up for 6 months - 40 months. Results Before extracorporeal circulation cardiac valve replacement, the aortic annulus diameter measured and detection rates of calcification by transthoracic echocardiography were smaller and lower than actual surgical pathology results ( P < 0. 05 ) . No differences in the results from transesophageal echocardiography of two-dimensional and three-dimensional examination when compared to pathological findings ( P > 0. 05 ) . Conclusions For patients receiving extracorporeal circulation cardiac valve replacement, transesophagus echocardiography examination is helpful to guide the selection of valve stent and operation methods.

Objective To explore the curative effect of triple lumen feeding tube on upper gastroin-testinal tract perforation after endoscopic operation.Methods A total of 1 12 patients with upper gastrointes-tinal perforation after endoscopic operation were involved in this prospective control test.The remission rate of abdominal pain and abdominal distension,gastric pH,lesion healing time,length of stay and the success rate of conservative treatment were compared between the control group (indwelling traditional gastric tube and intravenous use of omeprazole injection)and the experimental group (intestinal administration of PPI through triple lumen feeding tube).Results The remission rate of abdominal pain of the experimental group was 61. 3% at 6 hours,and 83. 2% at 12 h,which were significantly higher than those of the control group (P<0. 05 ).The lesion healing time(of cases where lesions were not clipped)and length of hospitalization of the experimental group were significantly shorter than those of the control group(P<0. 05 ).The gastric pH and success rate of conservative treatment of the two groups had no significant difference(P>0. 05 ).Con-clusion The curative effect of triple lumen feeding tube on upper gastrointestinal tract perforation after endo-scopic operation is ideal.

Objective To study the influence of hierarchical model on the professional attitude and quality improvement of nurses and supply references for the apphcation of hierarchical model.Methods 310 nurses were divided into the control group(158 cases)and the practice group(152 cgses).The practice group adopted hierarchical model while the control group did not.Investigation was carried out in 15 aspects such as training requirement,choosing of nursing posts and departure intention,etc.Results The nursing teaching pest and nursing management post in the aspect of post requirement between the two groups had no statistical difference(P＞0.05),but statistical difference existed in other items(P＜0.05).In the aspect of training requirement evident difference existed in two groups(P＜0.05).Conclusion Application of hierarchical model proved to be a pivotal method to improve the professional passion and loyalty of nurses.

Objective To study the correlation between micrometastasis and human manmmaglobin mRNA(hMAM-mRNA)in tlle peripheral blood of the patients with breast cancer.Methods Peripheral blood samples were obtained from 92 patients with breast carcinoma,and 20 patients were with benign breast diseases,and 10 patients with other system malignant tumor,and 10 normal healthy volunteers.To identify breast carcinoma cells in periphersl blood,hMAM-mRNA Was amplified from total RNA extracted from whole blood by nested reverse transcription-polymerase chain reaction(Nested-RT-PCR).The expression of hMAM-mRNA wag contracted to MCF-7 breast cancer cell.Results The hMAM-mRNA wag only detectable in the blood from breast disease patients.Among 92 breast carcinoma patients,40 were nested-RT-PCR positive for hMAM-mRNA.The positive rate was 43.5%(40/92).The expression of hMAM-mRNA in pe-ripheral blood was not correlated with patient's estrogen or pregnant receptor;menstruation state;primarytumour size.The expression of hMAM-mRNA in peripheral blood wag correlated with patient's age:the state of c-erbB-2 and carcino embryonic antigen and carbohydrate antigen 15-3.Conclusion The hMAM-mRNA in peripheral blood may be indicator of the micmmetastasis of breast cancer.