1.Dembo polymerase chain reaction technique for detection of bovine abortion, diarrhea, and respiratory disease complex infectious agents in potential vectors and reservoirs
Sayed Samim RAHPAYA ; Shinobu TSUCHIAKA ; Mai KISHIMOTO ; Mami OBA ; Yukie KATAYAMA ; Yuka NUNOMURA ; Saki KOKAWA ; Takashi KIMURA ; Atsushi KOBAYASHI ; Yumi KIRINO ; Tamaki OKABAYASHI ; Nariaki NONAKA ; Hirohisa MEKATA ; Hiroshi AOKI ; Mai SHIOKAWA ; Moeko UMETSU ; Tatsushi MORITA ; Ayako HASEBE ; Keiko OTSU ; Tetsuo ASAI ; Tomohiro YAMAGUCHI ; Shinji MAKINO ; Yoshiteru MURATA ; Ahmad Jan ABI ; Tsutomu OMATSU ; Tetsuya MIZUTANI
Journal of Veterinary Science 2018;19(3):350-357
Bovine abortion, diarrhea, and respiratory disease complexes, caused by infectious agents, result in high and significant economic losses for the cattle industry. These pathogens are likely transmitted by various vectors and reservoirs including insects, birds, and rodents. However, experimental data supporting this possibility are scarce. We collected 117 samples and screened them for 44 bovine abortive, diarrheal, and respiratory disease complex pathogens by using Dembo polymerase chain reaction (PCR), which is based on TaqMan real-time PCR. Fifty-seven samples were positive for at least one pathogen, including bovine viral diarrhea virus, bovine enterovirus, Salmonella enterica ser. Dublin, Salmonella enterica ser. Typhimurium, and Neospora caninum; some samples were positive for multiple pathogens. Bovine viral diarrhea virus and bovine enterovirus were the most frequently detected pathogens, especially in flies, suggesting an important role of flies in the transmission of these viruses. Additionally, we detected the N. caninum genome from a cockroach sample for the first time. Our data suggest that insects (particularly flies), birds, and rodents are potential vectors and reservoirs of abortion, diarrhea, and respiratory infectious agents, and that they may transmit more than one pathogen at the same time.
Animals
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Birds
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Cattle
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Cockroaches
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Diarrhea Viruses, Bovine Viral
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Diarrhea
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Diptera
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Disease Reservoirs
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Disease Vectors
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Enterovirus
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Enterovirus, Bovine
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Genome
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Insects
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Neospora
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Polymerase Chain Reaction
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Real-Time Polymerase Chain Reaction
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Rodentia
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Salmonella enterica
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Virulence Factors
Result Analysis
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