Three cases of spondyIolysis were experienced and treated by anterior interbody fusion of the involved spines at the department of orthopaedic surgery in the Taegu Hospital One case was accompanied with severe lumbago with radiating pain and other two cases with lumbago significantly. Review of literature was done with report of three cases of spondylolysis.
Daegu ; Low Back Pain ; Spinal Fusion ; Spine ; Spondylolysis

No abstract available.
B-Lymphocytes* ; Cytokines* ; Humans*

No abstract available.
Blood Pressure* ; Metoclopramide* ; Rabbits*

No abstract available.
Abortion, Habitual* ; Female ; Immunization* ; Lymphocytes* ; Pregnancy

BACKGROUND AND OBJECTIVES: The Herpes Simplex type 2 Defective Infectious Single Cycle virus (DISC virus) is an attenuated virus originally produced as a viral vaccine, but it also serves as an efficient gene transfer vehicle. The main goals of this study were to examine determinants of gene transfer using DISC vectors for squamous cell carcinoma and to evaluate the efficacy of vaccination with the DISC virus carrying a combination of immunomodulatory genes (IL-2, GM-CSF) as cancer therapy in a model of squamous cell cancer in the C3H/HeJ mice. MATERIALS AND METHODS: We determinated the gene and protein expression of DISC-IL-2 and DISC-GM-CSF transfected SCCVII cells by RT-PCR and ELISA method. Also, we evaluated the ex vivo vaccination effects of DISC-IL-2 and DISC-GMCSF on preventing the development of SCCVII tumor. RESULTS: SCCVII cells transduced by the DISC virus vector (MOI=10) carrying the IL-2, or the GM-CSF gene, produced nanogram quantities of IL-2 or GM-CSF per 10(6) cells. Of particular interest was the observation that cells irradiated at different doses (5,000 cGy, 10,000 cGy) secreted levels of GM-CSF or IL-2 that were comparable to non-irradiated cells. In vivo vaccination using tumor cells transduced ex vivo with DISC-IL-2 or DISC-GMCSF resulted in protection against subsequent tumor challenge (p<0.01). Among the multiple immunomodulatory transgenes vaccination groups, the DISC-GMCSF transfected vaccine showed the greatest suppression of tumor development and growth (p<0.001). CONCLUSION: These data demonstrate that: 1) The DISC virus vector is capable of efficient gene transfer to SCCVII cells, 2) The GM-CSF secreting, genetically modified tumor vaccine (SCCVII/GMCSF) efficiently protected against tumor cell challenge and suppressed tumor growth in our tumor model. The DISC virus-mediated, cytokine gene transfer may prove to be useful in clinical therapy for head and neck cancers.
Animals ; Carcinoma, Squamous Cell ; Enzyme-Linked Immunosorbent Assay ; Genetic Therapy* ; Granulocyte-Macrophage Colony-Stimulating Factor* ; Growth and Development ; Head* ; Herpes Simplex ; Interleukin-2* ; Mice ; Neck* ; Neoplasms, Squamous Cell* ; Transgenes ; Vaccination

The pleckstrin homology (PH) domain is a protein module of approximately 100 amino acids, that has been found in signaling molecules, including serinelthreonine kinase, GTPase-activating protein, phospholipase, and some cytoskeletal proteins. Although the specific function of PH domain has not been defined yet, it is believed that this domain is involved in the regulation of signal transduction pathway. The expression plasmids of human PLCg PH domains were constructed to see the roles of them in IL-6 signal transduction. When these expression plasmids are transfected into B9 cells, only N-terminal of PH domain inhibited IL-6-induced B9 cell proliferation. These results suggest that N-terminal of PH domain is critical for IL-6 signal transduction in B9 cells. To search the binding proteins associated PH domains of PLCy1 in B9 cells, Glutathione S-trnaferase (GST) fusion proteins containg PH domains were expressed in E. coli. Then, IL-6-dependent B9 cells were treated with 10 unit/ml IL-6 and the cell lysates were immunoprecipited with GST-PH doman fusion proteins. In vitro kinase assay of immune complex demonstrated that p38 (38 KDa) protein was coprecipitated with NC fusion protein, but IL-6 had no additional effect on it. When S-methaionine labelled cell lysates were used for immunoprecipitation, the same result was observed, conforming the association of p38 with NC motive of PH domain.
Amino Acids ; Antigen-Antibody Complex ; Carrier Proteins ; Cell Proliferation ; Cytoskeletal Proteins ; Glutathione ; GTPase-Activating Proteins ; Humans ; Hydrogen-Ion Concentration ; Immunoprecipitation ; Interleukin-6 ; Phospholipases ; Phosphotransferases ; Plasmids ; Signal Transduction

BACKGROUND: Hemopoietic cells require the constant presence of growth factors for survival in vitro and in vivo. Caspases have been known as central executors of apoptotic cell death. We have, therefore, investigated the pathways that regulate caspase activity and apoptosis using the CD34+ cell line, TF-1 which requires GM-CSF for survival. METHODS: Apoptosis was measured by annexin V staining and mitochondrial membrane potential was measured by DiOC6 labelling. Intracellular pH was measured using pH sensitive fluorochrome, BCECF or SNARF-1, followed by flow cytometry analysis. Caspase activation was analyzed by PARP cleavage using anti-PARP antibody. RESULTS: Removal of GM-CSF induceed PARP cleavage, a hallmark of caspase activity, concomitant with pHi acidification and a drop in mitochondrial potential. Treatment with ZVAD, a competitive inhibitor of caspases, partially rescued cell death without affecting pHi acidification and the reduction of mitochondrial potential, suggesting that both these events act upstream of caspases. Overexpression of Bcl-2 prevented cell death induced by GM-CSF deprivation as well as pHi acidification and the reduction in mitochondrial membrane potential. In parental cells maintained with GM-CSF, EIPA, a competitive inhibitor of Na+/H+ antiporter induced apoptosis, accompanied by a drastic reduction in mitochondrial potential. In contrast, EIPA induced apoptosis in Bcl-2 transfectants without causing mitochondrial membrane depolarization. CONCLUSION: Taken together, our results suggest that the regulation of H fluxes, either through a mitochondrion- dependent or independent pathway, is central to caspase activation and apoptosis.
Annexin A5 ; Apoptosis* ; Caspases ; Cell Death ; Cell Line ; Flow Cytometry ; Granulocyte-Macrophage Colony-Stimulating Factor* ; Humans ; Hydrogen-Ion Concentration* ; Intercellular Signaling Peptides and Proteins ; Ion Transport ; Membrane Potential, Mitochondrial ; Mitochondrial Membranes ; Parents

No abstract available.
Adult* ; Humans ; Neck*

It is usually difficult to obtain an exact reduction of bony mallet finger involving more than one third of the articular surface by closed manipulation and extension splinting and needs the operative treatment to obtain an exact anatomical reduction. Operative treatment of a mallet finger is a difficult procedure, fraught with many potential problems, including intraoperative fragmentation of dorsal lip fracture, difficulty of exposure and anatomical reduction of the fragment, skin slough, and postoperative loss of fixation. The purpose of this study is to evaluate the fixation technique and to reduce intraoperative complications. We retrospectively reviewed 13 patients of bony mallet fingers, who were treated by tension wire fixation between January 1994 and August 1995 at the Department of Orthopaedic Surgery, Kwangmyung Sung Ae Hospital. There were 6 male and 7 female pateints. The most common cause was sports injury (6 cases). According to Wehbe and Schneider classification, 8 cases were type II and subtype B, 7 cases were the other types. According to modified Abouna and Brown criteria, the clinical results were success in 10 cases and failure in 3 cases. Postoperative complications were superficial infeciton (2 cases), skin necrosis (1 case), nail deformity (1 case) and secondary extensor tendon rupture (1 case). In conclusion, tension wire fixation of bony mallet finger was a simple and effective fixation of the fragment under the direct vision, which make it possible to exercise early and fix sturdily. But it should be avoided some pitfalls associated with internal fixation of small bony fragments.
Athletic Injuries ; Classification ; Congenital Abnormalities ; Female ; Fingers* ; Humans ; Intraoperative Complications ; Lip ; Male ; Necrosis ; Postoperative Complications ; Retrospective Studies ; Rupture ; Skin ; Splints ; Tendons

BACKGROUND AND OBJECTIVES: Little information about the surgical anatomy and technique for retropharyngeal node dissection has been published. The purpose of this study was to review our surgical technique and results of retropharyngeal lymph node dissection. MATERIALS AND METHODS: Eleven advanced oropharyngeal and hypopharyngeal squamous cell carcinoma patients who had been treated with resection of primary tumor and standard neck dissection including retropharyngeal lymph node dissection from 1994 to 1999 were evaluated retrospectively. RESULTS: One of 11 patients had positive retropharyngeal lymph node. The surgical technique used for retropharyngeal lymph node dissection were total laryngopharyngectomy, mandibular splitting or mandibulectomy approach. There was no specific complication of retropharyngeal lymph node dissection except one case of Horner's syndrome. CONCLUSION: Retropharyngeal lymph node dissection was a safe procedure, bet it required total laryngopharyngectomy, madibular splitting or mandibulectomy approach. It was possible to remove retropharyngeal lymph made en-bloc with primary tumor in most cases.
Carcinoma, Squamous Cell ; Horner Syndrome ; Humans ; Hypopharyngeal Neoplasms ; Lymph Node Excision* ; Lymph Nodes* ; Neck Dissection ; Oropharyngeal Neoplasms ; Retrospective Studies