This study was aimed to elucidate the relationship between combinations of treadmill speed-grade and oxygen consumption(Vo2). Twenty athetic and 20 non-athletic male college students aged 19-24yr were employed to exercise on a treadmill using 4 speeds(4.02, 5.47, 6.76 and 8.05km/h) and 5 grades(0, 8, 12, 16 and 20%). A fixed speed was selected for each session with the grade increased every 3 min. The Vo2, heart rate, stride frequency and stride length were measured during the last min of each 3-min stage. Vo2increased linearly with increasing speed and grade showing significant multiple correlations in nonathletes(Vo2=3.64x+0.831y+0.031xy-7.03, R=0.98, P<0.01) and athletes(Vo2=3.48x+0.324y+0.112xy-5.74, R=0.99, P<0.01). Stride frequency and length tended to increase with increasing speed except for the transition from walking to runnig at 8.05Km/h at which the stride frequency ran up much higher with the stride length getting lower than at 6.76Km/h. Heart rate increased linearly with increasing Vo2. The rate of increase was higher during walking than during running. These results indicate that athletes have higher rate of increase in Vo2than nonathletes at near-maximal exercise and may be used as a guideline in predicting maximum oxygen comsumption and in prescribing exercise intensity.
Athletes* ; Heart Rate ; Humans ; Male ; Oxygen Consumption* ; Oxygen* ; Running ; Walking

No abstract available.
Animals ; Cricetinae* ; Mice*

PURPOSE: To investigate the effect of estrogen on the expression of calcium-activated potassium (KCa) channels in an overactive bladder rat model. To this end, mRNA and protein levels of KCa channel subtypes in the bladder of ovariectomized rats were measured by reverse transcription polymerase chain reaction and western blotting, respectively. METHODS: Ten-week-old female Sprague-Dawley rats were divided randomly into 3 groups: sham-operated control group (n=11), ovariectomy group (n=11), and the group treated with estrogen after ovariectomy (n=12). Rats in the last group were subcutaneously injected with 17β-estradiol (50 µg/kg) every other day for 2 weeks, whereas rats in the other 2 groups received vehicle (soybean oil) alone. Two weeks after treatment, the whole bladder was excised for mRNA and protein measurements. RESULTS: Protein levels of the large-conductance KCa (BK) channels in the ovariectomy group were 1.5 folds higher than those in the sham-operated control group. However, the protein levels of the other KCa channel subtypes did not change significantly upon bilateral ovariectomy. Treatment with 17β-estradiol after ovariectomy restored BK channel protein levels to the control value. In contrast, BK channel mRNA levels were not significantly affected by either ovariectomy alone or 17β-estradiol treatment. The small-conductance KCa type 3 channel (SK3) mRNA and protein levels decreased to 75% of control levels upon 17β-estradiol treatment. CONCLUSIONS: These results suggest that 17β-estradiol may influence urinary bladder function by modulating BK and SK3 channel expression.
Animals ; Blotting, Western ; Estrogens ; Female ; Humans ; Models, Animal ; Ovariectomy ; Polymerase Chain Reaction ; Potassium ; Potassium Channels, Calcium-Activated* ; Rats* ; Rats, Sprague-Dawley ; Reverse Transcription ; RNA, Messenger ; Urinary Bladder* ; Urinary Bladder, Overactive

No abstract available.
Emergencies* ; Humans ; Prospective Studies*

No abstract available.
Beckwith-Wiedemann Syndrome*

Anatomy of the pancreaticobiliary ductal system is so variable that one should think of its normal anatomy as nonexistent. But exact location of the termination of the common bile duct in important to the biliary surgeon when performing transduodenal exploration of the common bile duct, to the physician and radiologist when diagnosing pancreaticobilijary disease by ERCP. We report a case of Lnomalous termination of the common bile duct into thii. duadenal bulb diagnosed by ERCP and operative cholangiogram.
Cholangiopancreatography, Endoscopic Retrograde ; Common Bile Duct*

PURPOSE: The aim of this study is to evaluate the sequential histologic changes and spermatogenesis of the testis after extracorporeal shock wave treatment (ESWT). MATERIALS AND METHODS: ESWT to right testis was applied on total 48 immature, 5-week-old Sprague Dawley rats. The rats were sacrficed immediately, at 1 day, 3 days, 1 week, 2 weeks and 4 weeks after ESWT, and each group consisted of 8 rats including 2 rats as control (without ESWT). Histologic changes of both testes were examined, and serial three or more parafin sections were obtained, and immunohistochemical stains for bcl-2 and Hsp 70 and TUNEL method for apoptosis were performed. RESULTS: Hemorrhage and destruction of seminiferous tubules which appeared immediately after ESWT were maximal at 1 day after ESWT and were seen absorption and atrophy at 1 week after ESWT. Even regenerating tubules were found at 2 weeks after ESWT, no significant histologic difference between both testes experimental and control groups were seen except for some regenerating tubules. The right testicular tissues, remote from the ESWT-targeted area, showed preserved spermatogenesis since the age of 5 weeks 3 days, and was presented no significant difference between experimental and control groups. Hsp 70 and bcl-2 expression and apoptosis were maximal at 1 day after ESWT and persisted to 3 day after ESWT. Weak and focal expression were noted at 1 week after ESWT, and mostly disappeared at 2 weeks after. CONCLUSIONS: Spermatogenesis of right testis except the targeted area was not diffrent compared with that of left testis and control group. Hsp-70 expressin, which limits tissue destruction after injury, was immediately appeared for defense mechanism, and hemorrhage and tissue destruction following ESWT seemed to be due to apoptosis controlled by bcl-2 expression.
Absorption ; Animals ; Apoptosis* ; Atrophy ; Coloring Agents ; Hemorrhage ; In Situ Nick-End Labeling ; Rats* ; Rats, Sprague-Dawley ; Seminiferous Tubules ; Shock* ; Spermatogenesis ; Testis*

PURPOSE: The aim of this study was to examine how the mRNA and protein levels of calcium activated Kchannel (K(Ca)) and connexin (Cx) change in association with overactive bladder in the bladder mucosae of stress urinary incontinence (SUI) patients. MATERIALS AND METHODS: Twenty SUI patients were included in our study. Bladder mucosae were obtained, with using cold cup biopsy forceps, from the patients suffering with genuine stress urinary incontinence (group 1, n=7), from the patients suffering with SUI along with urgency and frequency (group 2, n=6), and from the patients suffering with mixed incontinence (group 3, n=7). RESULTS: The mRNA transcripts of type 2 (SK2) and type 3 (SK3) small conductance K(Ca), Cx26, and Cx43 were highly expressed in the bladder mucosa. The message of large conductance K(Ca)(BK) was significantly decreased in group 3 compared with that in the controls. The SK2 and Cx26 messages in group 3 were also lower than those in groups 1 and 2. In the presence of urge incontinence, the BK and SK2 protein levels were decreased and the Cx26 protein expression was significantly increased in the bladder mucosa of the SUI patients. In contrast, there were no significant differences in the mRNA and protein levels of K(Ca)s and Cxs between groups 1 and group 2. CONCLUSIONS: Downregulation of both BK and SK2 and upregulation of Cx26 in the bladder mucosa of MI patients may contribute to the alterations of urothelial instability, and this correlate with the symptom severity of bladder instability in SUI patients.
Biopsy ; Calcium ; Connexin 43 ; Connexins ; Down-Regulation ; Humans ; Mucous Membrane* ; Potassium Channels, Calcium-Activated* ; RNA, Messenger ; Surgical Instruments ; Up-Regulation ; Urinary Bladder* ; Urinary Bladder, Overactive* ; Urinary Incontinence* ; Urinary Incontinence, Stress ; Urinary Incontinence, Urge ; Urothelium

No abstract available.
Body Weight* ; Cholesterol*

PURPOSE: This study was conducted in order to investigate the protective effects of ethanolic extract of Acanthopanax koreanum Nakai (AE) against carbon tetrachloride (CCl4)-induced liver injury in rats. METHODS: Male Sprague-Dawley rats were randomly divided into four groups in order to receive the following experimental diets with intraperitoneal injection of CCl4 (2.0 mL/kg body weight, 20% solution 0.65 mL) for eight weeks (n = 8 per group): CCl4 control (CON), CCl4 + AE 1% (AE1), CCl4 + AE 3% (AE3), or CCl4 + acanthoic acid 0.037%, which is equivalent to AE 3% (AA). RESULTS: Highest serum ALT activity and albumin level were observed in the CCL4 control group, but showed a significant decrease by either AE or AA supplementation in a dose-dependent manner (p = 0.0063 and 0.0076, respectively). Both hemotoxylin and eosin staining and Masson's staining indicated remarkable prevention of CCl4-induced liver damage in the AE3 group. TNFalpha and IL-6 production were significantly lowered in the AE treated groups, but not in the AA group (p = 0.0016 and p = 0.0002, respectively). The effects of AE3 were greater than those of AA for inflammation and liver toxicity biomarkers. CONCLUSION: Taken together, the results suggested that ethanolic extract of Acanthopanax koreanum Nakai provided hepa-toprotective effects, leading to the reduction of inflammatory response. In addition, the effect of AE was superior to that of single compound AA.
Eleutherococcus* ; Animals ; Biomarkers ; Body Weight ; Carbon Tetrachloride ; Carbon* ; Diet ; Eosine Yellowish-(YS) ; Ethanol ; Hematoxylin ; Humans ; Inflammation ; Injections, Intraperitoneal ; Interleukin-6 ; Liver* ; Male ; Rats ; Rats, Sprague-Dawley* ; Tumor Necrosis Factor-alpha