1.The effect of interleukin 2 on the induction Of Nk 1.1 expression in CD8+ and CD4-CD8-T Cell.
Young Joo CHO ; Yoon Hae CHANG
Journal of Asthma, Allergy and Clinical Immunology 1998;18(2):290-298
BACKGROUND: Murine IL-2-induced lymphokine-activated killers can be divided into two mutually exclusive subset:NK1.1'CD8 and NK1.1 CD8+. However, there is a strong evidence that NK cell may belong to T cell lineage. Recently novel lymphocyte subsets, present in the adult murine thymus, CD3+NK1.1'TCRap(TNK) cell is readily identifiable in fresh obtained murine adult CD4 CD8 thymocytes. MATERIAL AND METHOD: We sorted out CD4 and CD8 (double negative.' DN) cells and CD8+ cells from murine spleen and cultivated these cells with IL-2. And the surface B220, CD8, NK1. 1 and cytopasmic NK1.1 was analysed simultaneously to see whether these cells can be switched to the other subtype of cells. RESULT: Purified DN cells were switched to several subtype of cells'. CD8'B220+(LAK cells), NK1.1'B220+(LAK cells), CD8 B220, cytoplasmic NK1.1+B220 cells. Purified CD8 cells were switched to CD8+B220' LAK cells and cytoplasmic NK1.1+ CD8+ B220+ and cytoplasmic NK1.1' CD8 B220 cells. In addition, the CD8' cells originated from DN cells do not express the cytoplasmic NK1.1 in contrary to the sorted CD8 cells. CONCLUSION: Our results indicated that these will be useful models to investigating CD8 precursor potentials in populations of CD4 CD8 (doble negative) cells and relationship of NK1.1 These results also supports the hypothesis that T cells and NK cells have same ontogeny and CD8 effector functions are potentially diverse and could be exploited by various conditions that switch off host protected cytolytic response. These model offer a way to study the molecular regulation of CD8 gene expression.
Adult
;
Cell Lineage
;
Cytoplasm
;
Gene Expression
;
Humans
;
Interleukin-2*
;
Interleukins*
;
Killer Cells, Lymphokine-Activated
;
Killer Cells, Natural
;
Lymphocyte Subsets
;
Spleen
;
T-Lymphocytes
;
Thymocytes
;
Thymus Gland
2.Histopathological Analysis of 9 Cases of Plexiform Schwannoma.
Nam Hoon CHO ; Jung Hoon YOON ; Hyeon Joo JEONG
Korean Journal of Pathology 1988;22(4):435-439
We present 9 cases of plexiform schwannoma examined at Severance hospital from January, 1980 to September, 1987. The predilection sites of plexiform schwannoma included head and neck including oral cavity. The mean age at the time of diagnosis was 32 years. The difference of sex ratio was not apparent. Histopathological findings revealed multiple round to oval nodules encapsulated by thin fibroconnective tissue with nuclear palisading and Verocay body in each nodule. There was mild to moderate cellular atypia in some area, but mitotic figure was not found. The Bielschowsky's silver stain did not demonstrate any axon within or outside of the nodule. The thin capsule was positive with reticulin stain.
3.The effect of transforming growth factor-beta on the expression of CD8 in the CTLL-2 cell line.
Soo Young PARK ; Yoon Hae CHANG ; Young Joo CHO
Journal of Asthma, Allergy and Clinical Immunology 2000;20(1):92-98
BACKGROUND: Transforming growth factor-beta(TGF-beta) has multiple regulatory effects on cells of the immune system, and it has been suggested that differentiation of lymphoid cells is influenced by low concentra tions of this cytokine. OBJECTIVES: The aim or this study was to investigate the role of TGF-beta in regulation of T cell growth and differentiation, and to compare this effect with that of other cell signals known to be important in T cell ontogeny. METHODS: We used the CTLL-2 cell line in the presence of IL-2. Surface phenotype expression was analysed to see whether these cells could be switched to the other subtype of cells. RESULT: Treatment of CTLL-2 cells with TGF-beta resulted in dose dependent growth inhibition and morphological changes. Curing routine passage, less than 5% of cells were CD8alpha positive, whereas 38% of cells expressed CD8alpha when treated with IL-2 plus TGF-beta. However, TPA plus calcium ionophore, IFN-gamma, or TNF-alpha caused no significant changes in the proportion of CD8 cells. CONCLUSION: Our results show that this experiment can be a useful model for investigating CD8 precursor potentials in populations of CD4-CD8-(double negative) cells, and such a model may offer a way to study the molecular regulation of CD8 gene expression.
Calcium
;
Cell Line*
;
Gene Expression
;
Immune System
;
Interleukin-2
;
Lymphocytes
;
Phenotype
;
Transforming Growth Factor beta
;
Tumor Necrosis Factor-alpha
4.A Case of Congenital Rubella Syndrome.
Kang Cheol YOON ; Kyung Hee MOON ; Chan Uhng JOO ; Soo Chul CHO ; Jung Soo KIM
Journal of the Korean Society of Neonatology 1998;5(1):104-108
We experienced a case of congenital rubella syndrome manifesting patent ductus arteriosus, petechiae, microcephaly, intrauterine growth retardation, and hepatomegaly in one-day-old female newborn infant. Congenital rubella syndrome was confirmed by positive results of anti-rubella IgM in the sera of patient. Anti-rubella IgM was negative in the sera of mother, while anti-rubella IgG was positive.
Ductus Arteriosus, Patent
;
Female
;
Fetal Growth Retardation
;
Hepatomegaly
;
Humans
;
Immunoglobulin G
;
Immunoglobulin M
;
Infant, Newborn
;
Microcephaly
;
Mothers
;
Purpura
;
Rubella Syndrome, Congenital*
5.Hypersensitivity myocarditi caused by ceused by cafadroxil.
Tae Rim SHIN ; Hae Young CHOI ; Yoon Hye CHANG ; Young Joo CHO
Korean Journal of Allergy 1997;17(2):186-191
Hypersensitivity myocarditis is an inflammatory disease of the myocardium usually related to drug allergy. The clinical manifestation may be nonspecific, and the diagnosis is seldom suspected or established during the life. So in most of the reported patients, the diagnosis was made at autopsy. Although, retrospectively, more than 90% of the patients described have had clinically recognizable cardiac involvement before death, tole clinical suspicion of hypersensitivity myocarditis is rare. The presence of nonspecific cardiac finding in a patient receiving any drug associated with hypersensitivity should alert the clinician to the possibility of drug related myocarditis. We experienced a case of hypersensitivity myocarditis accompanying allergic skin lesion in a 58 years old man with a history of taking cefadroxil. Fortunately, the outcome was favorable, therefore we do not have histologic confirmation of the diagnosis.
Autopsy
;
Cefadroxil
;
Diagnosis
;
Drug Hypersensitivity
;
Humans
;
Hypersensitivity*
;
Middle Aged
;
Myocarditis
;
Myocardium
;
Retrospective Studies
;
Skin
6.Advance in Estrogen Signaling through Estrogen Receptor.
Journal of Korean Society of Endocrinology 2004;19(1):10-18
No abstract available.
Estrogens*
7.A Case of Takayasu's Arteritis : Managed with Percutaneous Transluminal Angioplasty.
Hyeon Bu KIM ; Byoung Soo CHO ; Sung Ho CHA ; Joo Hyung OH ; Yup YOON
Korean Circulation Journal 1997;27(10):1044-1049
Takayasu's arteritis is a chronic vasculitic disease of the aorta, its major branches and the pulmonary arteries, resulting in stenosing, occlusive or aneurysmal lesion. While the precise etiology of Takayasu's disease is unknown, an autoimmune mechanism or active tuberculous inflammation have been suggested. It is more common in young oriental women but has a rare incidence in children. We experienced a case of Takayasu arteritis type IV in an 8years old girl. The diagnosis was made by physial examination and digital substraction aortography(DSA) which showed narrowing of left common carotid artery, right subclavin artery and left renal artery. The perfusion defect at posterior segment of right upper lobe was noted in lung perfusion scan. Medical treatment and percutaneous transluminal angioplasty(PTA) were performed. In addition to case report, a brief review of literature was added.
Aneurysm
;
Angioplasty*
;
Aorta
;
Arteries
;
Carotid Artery, Common
;
Child
;
Diagnosis
;
Female
;
Humans
;
Incidence
;
Inflammation
;
Lung
;
Perfusion
;
Pulmonary Artery
;
Renal Artery
;
Takayasu Arteritis*
8.Detection of Mycobacterium leprae in Skin Biopsy Sepcimens From Leprosy patients by Polymerase Chain Reaction.
Kyeong Han YOON ; Sang Nae CHO ; Jung Bok LEE ; Joo Deuk KIM
Korean Journal of Dermatology 1994;32(3):409-415
BACKGROUND: Polymerase chain reaction(PCR) has brought an oppotunity for rapid detection of Mycobacterium leprae in clinical pecimens for the diagnosis of leprosy. Th DNA segment specific to M. leprae was detectable in a matteir of hours and DNA from one orgnisa appeared positive by PCR. However, the PCR tool has not been evaluated using elinical specimeriis from leprosy patients and controls. OBJECTIVE & METHODS: The primers amplifying 372bp segment of rebetitive sequence of M. leprae DNA were used in PCR. Skin biopsy specimens from 102 leprosy patient, and controls were examined for the presence of M. leprae by PCR and the results were aomared with microscopic and histopathologic findings. RESULTS: 1. As a result, of PCR after DNA preparation of M. leprae, six other mycobacteria, ten other bacteria, and skin from leprosy with five other skin biopsy tissues, 372bp DNA fragment was specifically amplified from M. leprae. 2. Dot blot, hybridization of PCR products showed that the 372bp DNA from skin biopsy specimens were derived from M. leprae. 3. As a result of PCR after DNA preparation of 10-fold diluted M. legrae from mouse footpad, PCR gave a positive result as low as one organism. 4. Of 87 specimens in which acid-fast bacilli were found under microcopic examinations 97% had positive PCR results. 5. Of 97 specimens which hadihistopathologic evidences of leprosy 95% had positive PCR results. 6. Of 15 specimens in which acid-fast bacilli were not found under n!icroscopic examinations 73% had positive PCR results. In three of five cases which had neither histopathologic nor microscopic evidences of leprosy had positive PCR results. CONCLUSION: PCR method amplifying 372bp fragment of repetitive seqi,ence was highly sensitive and specific in detecting M. leprae DNA in skin biopsy specimens, thus may be a useful tool as an additive diagnostic method, espcially for cases where microscopic antihystopathologic findings are not definite.
Animals
;
Bacteria
;
Biopsy*
;
Diagnosis
;
DNA
;
Humans
;
Leprosy*
;
Mice
;
Mycobacterium leprae*
;
Mycobacterium*
;
Polymerase Chain Reaction*
;
Skin*
9.A Case of Bilateral Brown's Syndrome.
Journal of the Korean Ophthalmological Society 1987;28(3):709-714
Brown's syndrome is a clinical entity which shows restriction of elevation in the adducted position of the affected eye and in which the forced duction test for elevation in adduction is positive. The syndrome has been found to be bilateral in 10% of the cases. The authors experienced Brown's syndrome of both eyes associated with exophoria-tropia and both superior oblique overaction(SOOA OU) in an 18-year-old girl. We performed tenotomy of the superior oblique muscles in both eyes and 8mm recession of left lateral rectus muscle. After operation, orthophoria was achieved in primary, up and downgaze and SOOA of both eyes disappeared and there is only mild restriction of elevation in adduction in each eyes. The same findings were shown in follow up of 6 month after operation.
Adolescent
;
Female
;
Follow-Up Studies
;
Humans
;
Muscles
;
Tenotomy
10.Two Cases of Basal Cell Carcinoma Treated with Recombinant Alpha-2 Interferon.
Yoon Whoa CHO ; Joo Hyeup LEE ; Chang Kwun HONG ; Byung In RO
Annals of Dermatology 1990;2(2):141-144
Two patients with basal cell carcinoma (BCC) were treated with recombinant alpha-2 interferon. One patient was a 48-year-old man who had a 8 × 5 an sized ulcer with an elevated border on the face for 10 years, which revealed BCC of the solid type, histopathologically. He received intralesional injections of recombinant alpha-2 interferon (total dose: 51 × 10⁶ IU) for 11 weeks. The biopsy specimen after completion of injections showed a remaining small tumor cell nest in the lesion despite clinical remission. The other patient was a 75-year-old female, who had 1.2 × 1.2 cm-sized, darkly pigmented, nodular BCC of the solid type on the nose for 3 years. She also received intralesional injections of recombinant alpha-2 interferon (total dose: 18 × 10⁶ IU) for 8 weeks. The biopsy specimen after completion of injections did not show any tumor cells.
Aged
;
Biopsy
;
Carcinoma, Basal Cell*
;
Female
;
Humans
;
Injections, Intralesional
;
Interferons*
;
Middle Aged
;
Nose
;
Ulcer