1.THE NEW CRUSH MODEL AND ITS PATENCY RATE ON THE RAT FEMORAL VESSELS.
Journal of the Korean Society of Plastic and Reconstructive Surgeons 1997;24(2):257-263
A new crush anastomosis model which can be evaluated as crush energy was established on the femoral artery and vein of the rats weighing 250g on the average. The degrees of vessel injury were evaluated as crush energy. The arteries underwent the crush injury with the energy of 0.3J, 0.4J, 0.5J, while the reins were crushed with the energy of 0.2J, 0.3J, 0.4J respectively then the vessels in each group were divided and anastomoses by a standard microsurgical technique. Saline and heparin were used as a luminal topical agent and systemic one, which allowed contact with the damaged endothelium. The heparin treated group had a patency rate of 95%(0.3J), 87.5%(0.4J), 40%(0.5J) vs. 55%(0.3J), 15%(0.4J), 10%(0.5J) in the saline irrigated group in the femoral arteries, which had the patency rate of 82.5%(0.2J), 72.5%(0.3J), 42.5%(0.4J) vs. 35%(0.2J), 12.5%(0.3J), 7.5%(0.4J) in the saline irrigated group in the femoral vein at 1 day. There was a energy-related decrease in patency rate. These findings indicate that the crush model of 0.4J in the rat femoral artery, 0.3J in the rat femoral vein is a reliable and reproducible thrombosis model. The model described in this study is to be used to evaluate and assess the ability of antithrombotic agent in preventing thrombosis at the anastomotic site.
Animals
;
Arteries
;
Endothelium
;
Femoral Artery
;
Femoral Vein
;
Heparin
;
Phenobarbital
;
Rats*
;
Thrombosis
;
Veins
2.Morphological Change with the Induction of Cisplatin Resistance from the Bladder Cell Lines.
Korean Journal of Urology 2001;42(2):139-146
PURPOSE: When Managing metastatic bladder tumors, to overcome the resistance mechanism of cisplatin is a main problem to be solved. The objective is to confirm the changes of general and ultrastructural morph ology with the induction of cisplatin resistance from the bladder cell line. MATERIALS AND METHODS: The samples of this investigation are 2ng/ml-cisplatin resistant human bladder cell lines T24R2 established by SNUH Urology and the drug resistant bladder cell lines T24 was obtained from ATCC, as a control group. We cultured the resistant cell line on the slide and observed it using light microscopy to see the general morphology. For the ultrastructural morphology, we fixed cultured cells, made an epon block, sliced an ultrathin section and observed it using H-71000 EM. RESULTS: Under light microscopy, the cytoplasm of the resistant cell line shows a plumper pattern than that of the parent cell. Under electronmicroscopy, the chromatin of the resistant cell line has a relatively finely dispersed chromatin pattern when compared to the parent cell line, which shows a coarse and aggregated chromatin pattern. Within the cytoplasm, the mitochondrial volume, dilated rough endoplasmic reticulum, polyribosomes and ribosomes are moderately increased in the resistant cell line when compared to the parent cell line. In particular, we found a great amount of double membrane vesicle near the cell surface and pinocytic vesicles on the surface, which are seldom observed within the parent cells. CONCLUSIONS: We concluded that the cisplatin resistant human bladder cell lines (T24R2) underwent a morphological change with the induction of cisplatin resistance, and we hypothesize that the resistant cell's ultrastructure, which shows morphological change, will be involved in the drug resistance mechanism. Regarding this matter, further research will be needed.
Cell Line*
;
Cells, Cultured
;
Chromatin
;
Cisplatin*
;
Cytoplasm
;
Drug Resistance
;
Endoplasmic Reticulum, Rough
;
Humans
;
Membranes
;
Microscopy
;
Mitochondrial Size
;
Parents
;
Polyribosomes
;
Ribosomes
;
Urinary Bladder Neoplasms
;
Urinary Bladder*
;
Urology
4.Effect of All-trans-retinoic Acid on the Growth and cAMP Level in Cultured Normal Human Melanocytes Stimulated by a-MSH.
Korean Journal of Dermatology 1999;37(8):1017-1028
No abstract available.
Humans*
;
Melanocytes*
;
Tretinoin*
5.Microtia correction using autogenous rib cartilage grafts.
Taik Jong LEE ; Young Jin SHIN ; Yoon Joo LEE
Journal of the Korean Society of Plastic and Reconstructive Surgeons 1992;19(5):837-846
No abstract available.
Cartilage*
;
Ribs*
;
Transplants*
6.Relationship of compliance and oxygen transport in experimental acute respiratory failure during positive end-expiratory pressure ventilation.
Sang Do LEE ; Se Jin YOON ; Bok Hee LEE
Tuberculosis and Respiratory Diseases 1993;40(1):6-15
No abstract available.
Compliance*
;
Oxygen*
;
Positive-Pressure Respiration*
;
Respiratory Insufficiency*
;
Ventilation*
7.Relationship of compliance and oxygen transport in experimental acute respiratory failure during positive end-expiratory pressure ventilation.
Sang Do LEE ; Se Jin YOON ; Bok Hee LEE
Tuberculosis and Respiratory Diseases 1993;40(1):6-15
No abstract available.
Compliance*
;
Oxygen*
;
Positive-Pressure Respiration*
;
Respiratory Insufficiency*
;
Ventilation*
8.Clinical menifestations of tuberculosis in chronic renal failure.
Hyung Jin YOON ; Yoon Goo KIM ; Curie AHN ; Jin Suk HAN ; Auhnggwon KIM ; Jung Sang LEE
Korean Journal of Nephrology 1991;10(3):352-359
No abstract available.
Kidney Failure, Chronic*
;
Tuberculosis*
9.Hypnotics and cognitive function.
Bong Joo OH ; Jin Sang YOON ; Hyung Yung LEE
Journal of Korean Neuropsychiatric Association 1992;31(4):756-766
No abstract available.
Hypnotics and Sedatives*
10.A Case of Primary Erythermalgia.
Byeong Song LEE ; Chee Won OH ; Tae Jin YOON
Annals of Dermatology 1997;9(3):224-227
An 18-year-old man had suffered from intense burning with redness and an increased temperature of both the lower legs, forearms and hands since the age of 5. Symptoms were exacerbated by exposure to heat or walking. The patient obtained temporary relief by immersing his feet and hands in cold water. We observed the increase of skin temperature in relation to pain by thermography. No specific abnormalities were noted in laboratory studies. Treatment with aspirin was ineffective. We report a rare case of primary erythermalgia with typical features.
Adolescent
;
Aspirin
;
Burns
;
Erythromelalgia*
;
Foot
;
Forearm
;
Hand
;
Hot Temperature
;
Humans
;
Leg
;
Skin Temperature
;
Thermography
;
Walking
;
Water