Eight bacterial strains were examined whether they have phoP/phoQ genes which were known to be involved in the intracellular survival of Salmonella typhimurium. The phoP/phoQ operon were known to sense the stimuli of the genes involved in the adaptation of the environment. Using 514-basepairs EcoRV DNA fragment of phoP region of Salmonella typhimurium as a probe, dot blot hybridization were performed. Chromosomal DNAs of Klebsiella pneumonia, Pseudomonas aeruginosa, Serratia marscescens, Enterobacter cloacae, Salmonella typhimurium, Escherichia coli, Shigella dysenteriae, and Listeria monocytogenes were examined by DNA hybridization assay. Against our expectation, intracellular pathogen, L. monocytogenes, did not have similar DNA sequences to phoP/phoQ of S. typhimurium, while E, coli, S. dysenteriae, and E. cloacae showed the positive signal even though they were not intracellular pathogens. This result suggested that the phoP/phoQ operon was absent in intracellular pathogenic bacterias other than S. typhimurium. Rather it was found in phylogenetically closer bacterias to S. typhimurium, which were not able to survive in intracellular environment. Some different mechanism, which is not dependent on phoP/phoQ operon, could be involved in the intracellular survival of L. monocytogenes.
Bacteria* ; Base Sequence ; Cloaca ; DNA ; Enterobacter cloacae ; Escherichia coli ; Klebsiella ; Listeria monocytogenes ; Operon ; Pneumonia ; Pseudomonas aeruginosa ; Salmonella typhimurium ; Serratia ; Shigella dysenteriae

ACL plays an important role in stability of knee joint and its injury causes instability of joint which is known as the initiation of problem knee. Reconstruction of the anterior cruciate ligament would be preferred to the primary repair in treatment. And recently with the advance of arthroscopic technique and development of bone-patellar tendon-bone (BPB) graft material, the result of reconstruction is somewhat superior to that of primary repair. Authors compared the results of 20 primary repairs (Group I ) and 20 reconstructions (Group II) among 64 acute ACL injury treated Sung-Ae General Hospital from January 1989 to December 1994. The results were as follows: 1. Most common associated injuries were medial collateral ligament rupture and medial meniscal tear. 2. Group I showed postoperatively 13 negative anterior drawer test, 12 negative Lachmann test and 12 negative pivot shift test and group II showed postoperatively 17 negative anterior drawer test, 15 negative Lachmann test and 17 negative pivot shift test. (p<0.05) 3. In postoperative Lysholm knee scoring scale, group I showed 86 points and group II showed 93 points (p<0.05). 4. In postoperative KT-1000 arthrometer, 12 cases in Group I and 16 cases in Group II showed less than 2mm difference with sound side (p<0.05).
Anterior Cruciate Ligament ; Collateral Ligaments ; Hospitals, General ; Joints ; Knee ; Knee Joint ; Rupture ; Transplants

The fat embolism syndrome is a complication which encounters rarely after fractures of pelvis and long bones of lower extremity of severe injury of soft tissue, and may results in microinfarctions in lungs, brain, heart, kidneys and other important organs when fat globules flow from bone marrow into small vessels. And that is the one of the main cause of death within 72 hours after major trauma of long bones. Diagnosis of fat embolism syndrome was made by clinical criteria by Gurd, but, majority of cases didn't show characteristic clinical symptoms or signs. Recently CT and MRI of the brain are adopted to the diagnosis of FFS as a new method. Authors experiences six cases of fat embolism syndrome from Mar. 1991 to Mar. 1994 and analyzed the brain MRI findings. We concluded that the brain MRI findings such as diffuse brain swelling, petechial hemorrhage and microinfarctions were helpful to diagnose clinically apparent or subclinical fat embolism syndrome.
Bone Marrow ; Bones of Lower Extremity ; Brain Edema ; Brain ; Cause of Death ; Diagnosis ; Embolism, Fat ; Heart ; Hemorrhage ; Kidney ; Lung ; Magnetic Resonance Imaging ; Methods ; Pelvis

PURPOSE: The purpose of the study were to categorize the subjectivity of the quality of life in elderly women and to thereby understand the differences regarding quality of life desires. METHODS: This study used a Q-methodology to measure the quality of life in elderly women. A Q sample was collected from in-depth and objective interviews and WHOQLO. Fourteen participants rated 28 selected Q-statements on a scale of 1-7. The collected data was analyzed using PC Quanl. RESULTS: The principal component analysis identified 4 factors regarding the quality of life of elderly women. These categories were labeled 'hopelessness-oriented', 'relationship/adaptation-oriented', 'physical suffering-oriented', and 'self satisfaction-orientated'. CONCLUSION: Some of the factors regarding the quality of life of elderly women were determined through this research. Further research is necessary to set up and apply different the quality of life between men and women based on this result. The results of this study indicate that different approaches toward quality of life promotion programs are recommended based on the four factors of quality of life among elderly women.
Aged* ; Female ; Humans ; Male ; Principal Component Analysis ; Quality of Life*

No abstract available.
Down Syndrome* ; Humans ; Twins, Monozygotic*

No abstract available.
Retroperitoneal Fibrosis*

It was measured to check the state of health education by the survey of the items related to the health with first grade of students in middle school who mastered elementary courses. Totally 50 questions, which were selected from Standard and Dong-A reference books, were given to teachers and have got answered by the students who were totally 959(491 from urban areas and 468 from rural areas). It's done just after they entered middle school for a month, from April 10 to may 10, 1984. There were totally 782 items of health in all subjects of all grades. In contents, the most cases were about mental and emotional health(17.7%), exercise and rest(15.1%), environmental health, health life, personal health care, nutrition and foods, social health and organization, physiology and anatomy, statistics of public health population problem, disease and care, food sanitation, school health, parasitic and communicable disease control, eugenics and heredity(0.4%), etc were followed. In subjects, Korean language had 44.7% of mental emotional health in 114 cases, arithmetics 46.4% of statistics of public health in 26, sociology, 23.1% of environmental health in 118, natural science, 60.1% of physiology and anatomy in 30, ethics, 40.3% of mental and emotional health in 176, music, 21.8% of mental and emotional health and accidents in 23, art, 42.9% of exercise and rest in 28, physical education, 38.6% of exercise and rest in 201, practical course, 36.2% of nutrition and foods in 61, and there was nothing but only one case in Korean history. Subjects in total cases of health informing items are below: Physical education 25.8%, ethics 22.5%, sociology 15.1%, Korean language 14.6%, practical course 7.8%, natural science 3.8%, art 3.6%, arithmetics 3.3%, music 2.9%, Korean history 0.6%. Grades in total cases of health informing items are belows: the sixth grade 29.1%, the fourth grade 21.2%, the fifth grade 18.9%, the third grade 11.6%, the first grade 11.5%, the second grade 7.7%. The sections related to health matters were average 35.4%. According to the grades, the fourth and the sixth grade were 38.2% each other, the highest, and the second grade was 29.3%, the lowest. All sections in physical education included them. The acceptability to the knowledge for health was belows: 56.3% in urban students and 53.9% in rural students. There was some difference in acceptability between two parties (p<0.005).
Communicable Disease Control ; Delivery of Health Care ; Environmental Health ; Ethics ; Eugenics ; Health Education* ; Humans ; Music ; Natural Science Disciplines ; Physical Education and Training ; Physiology ; Public Health ; Pupil* ; Reference Books ; Sanitation ; School Health Services ; Sociology

PURPOSE: To report the clinical data and visual outcomes after treatment of patients with dysthyroid optic neuropathy (DON). METHODS: We retrospectively reviewed the medical records and orbital images of 40 patients (65 eyes) with DON and analyzed the visual outcomes after treatment with intravenous steroids pulse therapy, radiotherapy and orbital decompression. RESULTS: The study included 21 men and 19 women, with 10 (25%) being diabetic patients. Visual field test results revealed defects in 88.7% of DON eyes; afferent pupillary defects in 63.2%; reduced color vision in 78.5%; and abnormal visual evoked potentials in 84%. Orbital imaging showed moderate to severe apical crowding in 95% of the orbits and intracranial fat prolapse in 24.2%. Median best corrected visual acuity improved from 0.4 to 1.0 after one year of treatment (p < 0.001). We noted more improvement in vision with the use of decompressive surgery than with non-surgical methods (p < 0.05). Recurrences occurred in 7 patients who had not received orbital radiotherapy. CONCLUSIONS: Visual field defects and apical crowding seen on orbital imaging were the most sensitive indicators for the detection of DON. Treatment with intravenous steroids pulse therapy, radiotherapy and orbital decompression effectively improved visual outcomes in cases of DON.
Adult ; Aged ; Combined Modality Therapy ; *Decompression, Surgical ; Diabetes Complications ; Evoked Potentials, Visual ; Female ; *Graves Ophthalmopathy/pathology/surgery/therapy ; Humans ; Male ; Middle Aged ; Pulse Therapy, Drug ; Pupil Disorders ; *Radiotherapy ; Recovery of Function ; Recurrence/prevention & control ; Retrospective Studies ; Steroids/*administration & dosage ; Visual Acuity ; Visual Fields ; Young Adult

189-192 Six male patients with typical symptoms of Behcet's syndrome were subjected to a clinical examination. One of these was a Chinese patient. The age when the disease started was between 20 to 39 years. All of them became blind but no one was dead.
Asian Continental Ancestry Group ; Behcet Syndrome* ; Humans ; Male

Granular corneal dystrophy is primary heredofamilial dystrophy of the cornea, which is characterized by the presence of plemorphic milk-white spots in the superficial stroma in the central cornea. This corneal dystrophy was first ,described by Arthur Groenouw in 1890. Since then. many other types of familial corneal dystrophies have been discribed by morphological characteristics. In 1938 BUcklers classified the corneal dystrophy into 3 types; granular, macular and lattice dystrophy, whose nemenclature is now generally accepted. Now we report 2 cases of granular corneal dystrophies which involved 2 sisters in a family.
Cornea ; Humans ; Siblings