From May 1990 to July 1991. 11 bladder tumor patients were treated with Indiana continent ileocecal reservoir after radical cystectomy with pelvic lymphadnectomy and l0 were followed up. The average operation time for making pouch was 5 hours 22 minutes. The reservoir was created with reconfiguration of disrupted cecum and ileal patch and its average maximal capacity was 527. 8cc. The average interval of catheterization was 5 hours 36 minutes and the average catheterized urine volume was 357cc. The average intrareservoiral maximal pressure was 29.4cmH2O and the average intraluminal maximal pressure of plicated terminal ileal segment along with ilececal valve was 48cmH2O. All of 10 patients(100%) had successful daytime continence and 5 were able to sleep all night without catheteritation. Tunneled ureteral implantations along the tenia of the cecum were revealed no reflux on reservoirogram. The complications were 2 cases of temporary urine leakage From stoma site. 1 wound infection and 1 stomal bleeding. From the above results, although duration of followup is short, we can anticipate a superb outcome after experience further cases with this procedure, since successful continence and antireflux are achieved at this time.
Catheterization ; Catheters ; Cecum ; Cystectomy ; Follow-Up Studies* ; Hemorrhage ; Humans ; Indiana* ; Ureter ; Urinary Bladder Neoplasms ; Wound Infection

No abstract available.
Female ; Hypertension, Pregnancy-Induced* ; Iron* ; Pregnancy

Urethral polyp is an unusual cause of bladder outlet obstruction in male patients. Diagnosis is made by cystourethrography and is confirmed by transurethral biopsy. We report on an infant with a congenital polyp of the prostatic urethra with brief review of literature.
Biopsy ; Diagnosis ; Humans ; Infant ; Male ; Polyps* ; Urethra ; Urinary Bladder Neck Obstruction

BACKGROUND: The photsensitizing effect of quinolones has been recognized since their introdulation as an antibacterial agents. Recently several new second eneration antibacterial agents of this pharmacological class have become available for therapy, and are gaining increasing impotance. OBJECTIVE: To reveal the phototoxic potentials of some new quinolones by photohemolysis test, estimation of fluorescenc spectra, and Candida albicans test. METHODS: Nalidixic acid and four second-generation quinolones(ciprofloxacin, enoxacini, norfloxacin, and ofloxacitid were examined by fluorescence spertra which measured t.he phototoxc potentials by photochemial instability, photohemolsis test for the phototoxic properties against cell membranes and Candida tlbicans test for phototoxic properties against DNA. RESULTS: All drugs showed a fluorescence spectra within 360 nm to 450 nm, and in the photohemolysis test, all studied drug except ofloxacin got above 5% hemolytic value, and all drugs showed clear zone. in Candida albicans test after 48hours. CONCLUSION: These results suggested that all tested drugs were photochemically unstable. According to the mechanisris of cellular phototoxicity, ciprofloxacin, enoxacin, and norfloxacin was phtototoxic to nucleus and cell membrane, whereas ofloxacin was phototoxic to nucleus only.
Anti-Bacterial Agents* ; Candida ; Candida albicans ; Cell Membrane ; Ciprofloxacin ; Dermatitis, Phototoxic ; DNA ; Enoxacin ; Fluorescence ; Nalidixic Acid ; Norfloxacin ; Ofloxacin ; Quinolones

The gene encoding E7 oncoprotein of human papillomavirus type 16 was cloned and expressed in Escherichia coli, and the monoclonal antibodies (Mabs) against this expressed protein were generated. For the efficient immunization, two kind of recombinant E7 protein in fusion form were produced. One was maltose binding protein (MBP) fusion type (MBP-E7) and the other was T7 phage gene 10 product fusioa type (gene 10-E7). Immunization with these two fusion protein to mice, finally two Mabs (VD6 and IB10) were obtained. VD6 and IB10 showed reactivities with E7 protein in CaSki cell but not in HeLa by Western blot analysis. In addition, the Mab, VD6, reacted with COS-7 cell transfected with E7 gene majorly in cytoplasm by immunofluorescence test. Also VD6 could detect E7 protein in cytoplasm and nucleus of CaSki ceU by immunogold electron microscopy. Based on these results, the Mab VD6 was could be used for various E7 detection system such as Western blot analysis and immunohistochemical methods.
Animals ; Antibodies, Monoclonal* ; Bacteriophage T7 ; Blotting, Western ; Clone Cells ; COS Cells ; Cytoplasm ; Escherichia coli ; Fluorescent Antibody Technique ; Humans* ; Immunization ; Maltose-Binding Proteins ; Mice ; Microscopy, Electron

No abstract available.
Biopsy* ; Cytoplasm* ; Follow-Up Studies* ; Glomerulonephritis* ; Kidney Transplantation*

Intramucosal gastric cancer (IGC) is associated with a very low risk of lymph node metastasis; thus it is the main candidate for minimally invasive surgical procedures, such as endoscopic submucosal dissection (ESD). Herein, we document an extraordinary case of IGC, which showed a very aggressive clinical course. A 66-year-old female underwent ESD for early gastric cancer. Histologically, the tumor consisted mainly of moderately differentiated adenocarcinoma measuring 1.6 cm in diameter, and the tumor was confined to the mucosa. Despite annual esophagogastroduodenoscopic follow-up, the tumor recurred, with wide metastasis to multiple lymph nodes and bones throughout the body after three years. Fluorescence in situ hybridization study demonstrated MET gene amplification as well as low grade polysomy 7 in both original and recurrent tumors. The clinical characteristics of metastatic IGCs and the implication of MET amplification are discussed.
Adenocarcinoma ; Aged ; Female ; Fluorescence ; Follow-Up Studies ; Gene Amplification ; Humans ; In Situ Hybridization ; Lymph Nodes ; Mucous Membrane ; Neoplasm Metastasis ; Stomach Neoplasms* ; Surgical Procedures, Minimally Invasive

No abstract available.
Humans ; Iron* ; Peritoneal Dialysis, Continuous Ambulatory*

No abstract available.
C-Reactive Protein* ; Tocolysis*

Autologous Transfusion, storage of one's own blood for subsequent infusion if needed, is safe and effective in a variety of scheduled operative procedures. Obstetric involvement in such programs in very limited, however, because of concern over the possibility of inducing premature labor or causing fetal distress by blood volume change or vasovagal reactions. We describe our experience with pregnant women in this program. The incidence of vagovagal reactions of autologous donation was 9.5% (2.21). After entry into this program, 17pastients received a total 37pints, which consist of 19 Autologous and 18 Homologous. Homologous transfusion was avoided in 30% of patients receiving blood. The values of the mean hematocrits before and after hpebotomy were 34.1% and 31.8% respectively. It was stastically significant (p<0.01). We recommended that autologous blood donation by pregnant women in third trimester is safe for mothers or infants and it should be strongly encouraged for patient with placenta previa and repeated cesarean section.
Blood Donors ; Blood Volume ; Cesarean Section ; Female ; Fetal Distress ; Hematocrit ; Hemorrhage* ; Humans ; Incidence ; Infant ; Mothers ; Obstetric Labor, Premature ; Placenta Previa ; Pregnancy ; Pregnancy Trimester, Third ; Pregnant Women* ; Surgical Procedures, Operative