Diagnosis of mycobacterial infection is dependent upon the isolation and identification of causative agents. The procedures involved are time consuming and technically demanding. To improve the laborious identification process mycobacterial systematics supported by gene analysis is feasible, being particularly useful for slowly growing or uncultivable mycobacteria. To complement genetic analysis for the differentiation and identification of mycobacterial species, an alternative marker gene, rpoB encoding the B subunit of RNA polymerase, was investigated. rpoB DNAs (342 bp) were amplified from 52 reference strains of mycobacteria including Mycobacterium tuberculosis H37Rv (ATCC 27294) and clinical isolates by the PCR. The nucleotide sequences were directly determined (306 bp) and aligned using the multiple alignment algorithm in the MegAlign package (DNASTAR) and MEGA program. A phylogenetic tree was constructed with a neighborhood joining method. Comparative sequence analysis of rpoB DNA provided the basis for species differentiation. By being grouped into species-specific clusters with low sequence divergence among strains belonging to same species, all the clinical isolates could be easily identified. Furthermore RFLP analysis enabled rapid identification of clinical isolates.
Base Sequence ; Classification ; Complement System Proteins ; Diagnosis ; DNA ; DNA-Directed RNA Polymerases ; Mycobacterium tuberculosis ; Polymerase Chain Reaction* ; Polymorphism, Restriction Fragment Length* ; Residence Characteristics ; Sequence Analysis*

The solitary type of Koratoacanthoma is a common, rapidly growing, benign tumor with a natural history of spontaneous involution which was first described by Hutchinson in 1889. It was well known that this Keratoacanthoma is close resemblence to squamous cell carcinoma clinicallt & histopathology. The etiology of Kertoacanthoma is ucertain but it have been associated with many exogenous factors, including sun exposure, occupational exposure to heat and trauma, and tar and with autoimmune etiology and viral infection. We experienced 2 cases of soitary type of Keratoacantoma with typical clinical and histopathologic findings which presented on the pubic area and forehead but had no predisposing factors of Keratoacanthoma. The one was 66-year-old woman who had been a history of proritic, rapidly growing tumor on Mons Pubis since about 6 months ago. The another one was 40 year-old male who had been a history of asymromatic, growing tomor on forehead since about 3 months ago. In the H-E stained sections, there were horn filled invagination, dyskeratotic cell, horn pearl in the epidermis and an inflammatory infiltration in dermis. Diagnosis was cinfirmedby the clinical and histopatholigical pictures. The former was treated by electrocautery after simple excision and the latter by curretage and electrodesiccation.
Adult ; Aged ; Animals ; Carcinoma, Squamous Cell ; Causality ; Dermis ; Diagnosis ; Electrocoagulation ; Epidermis ; Female ; Forehead ; Horns ; Hot Temperature ; Humans ; Keratoacanthoma* ; Male ; Natural History ; Occupational Exposure ; Solar System

No abstract available.
Environmental Monitoring* ; Health Promotion*

lt is generally beIieved that several chemical mediators such as histamine, kinine, prustaglandin E and lysosomal enzyme are related to the pathogenesis of sunburn, but the exact mechenism of erythema is unknown. Recently, prostaglandins appear to be important mediators of ultraviolet erythema (UV-erythema) and several investigators has reported that the nonsteroidal antiinflammatory druga and corticosteroids supressed UV-erythema by inhibition of prostaglandin synthesis. This study was undertaken to evaluate the effect of topical nonsteroidaI antiinflammatary drugs and corticosteroids on UV-erythema induced by a artificial sunlamp. Nonsteroidal anti-inflammatory drugs (5% Bufexamac cream, 5% Ibuprofen cream, 5% Indomethacin cream) and corticosteroids(0.05% Fluocinonide cream, 0.5% Fluocortolone oint., 1%, Hydrocortisone cream) were applied immediately after 3~4 minimal erythema dose(MED) irradiation on back skin of 40 healthy volunteers and the delayed UV-erythema response was evaluated at 2, 4, 6, 8, 12, 24, 48 and 72 hours after irradiation, respectively. The resulta were as follows; 1) Minimal erythema dose(MED) was noted 4min. (62.5%), 3min. (25.0%), 5min. (22.5%). The averaged MED was 3. 63min. 2, MED has to be increased on site, applied nonsteroid and steroid agents topically, comparing with control site. 3) Blanching effect was detected in all cases of nonsteroid and steroid agents applicated topically, but in control site. 4) Average score in blanching effect was highest in 5% Indomethacin cream among nonsteroid agents(average score-2. 47) and 1% Hydrocortisone cream (average scoro -2. 63) among steroid agents.
Adrenal Cortex Hormones ; Bufexamac ; Erythema* ; Fluocinonide ; Fluocortolone ; Healthy Volunteers ; Histamine ; Humans ; Hydrocortisone ; Ibuprofen ; Indomethacin ; Prostaglandins ; Research Personnel ; Skin ; Sunburn ; Ultraviolet Rays*

Tuberculosis of the skin may present clinically as plaques, ulcers, verrucous, lesions, nodules, tumors, vegetatives reactions, and cicartricial infiltration. The reaction of the host to the presence of Mycobacterium tuberculosis also varies with the extent and the degree of invasion, the virulence of the bacilli, and nonspecific factors such as age and generaI state of health. The incidence of cutaneous tuberculosis has tendency to decline in the world because of thc availability of effective antiTB drugs, elimination of infected milk herbs, and an elevation of living standards, but in Korea, cutaneous tuberculosis remained to be a significant medical problem. We experienced 21 year-old male who had a case of 3 clinical types including Lupus vulgaris, Scrofuloderma, Papulonecrotic tuberculid. Diagnosis of 3 clinical types vere established by clinical history and signs, histo pathology, tuberculin test and effective of treatment vith INH, streptomycin and literature were reviewed.
Diagnosis ; Dronabinol ; Humans ; Incidence ; Korea ; Lupus Vulgaris ; Male ; Milk ; Mycobacterium tuberculosis ; Pathology ; Skin ; Socioeconomic Factors ; Streptomycin ; Tuberculin Test ; Tuberculosis ; Tuberculosis, Cutaneous* ; Ulcer ; Virulence ; Young Adult

Malignant melanoma is a tumor arising within the melanocyte system of the skin, eyes and juxtacutaneous mucous membrane. Tbrough the lymphatic channel developed early metastasis and regional lymphade- nopzthy developed. Malignant melanoma may arise on apparently normal skin, in a junction nevua, rareh in a compound nevus and extremeIy rarely in an intradermaI nevus and 20% of total incidence of malignant melanoma are preceded by a nevocellular nevus. A 41 year-old male patient who had multiple, pea to walnut sized, black colored. nodules developing on entire body visited our Dermto. dept. About one year ago, solitary black colored nodule arose from preexisting pigmented nevus which had been from 20 yrs ago on Rt. ant. axillary area and then nodule remcved by wide exeision and regional lymphadenectomy at a Hosp. 2 months after surgical operation, multiple pea to walnut sized, black colored nodules has developed on entire body. There was no lymphadenopathy on entire body. In H-E & Fontana-Masson stain, numerous bizzarre large cells with hyperchromatic variable sized melanin pigments are seen in the dermis and subcutaneous tissues Mitotic figutes are present. Diagnosis was confirmed by the clinical and histological feature. The patient was treated with BCG waccination(4*10^6-8*10^6 live organisms weekly) by altemately intralesional inj, on the lesion and subcuraneous inj. on both shoulder during 6wks and has been in follow-up.
Adult ; Ants ; Dermis ; Diagnosis ; Follow-Up Studies ; Humans ; Incidence ; Juglans ; Lymph Node Excision ; Lymphatic Diseases ; Male ; Melanins ; Melanocytes ; Melanoma* ; Mucous Membrane ; Mycobacterium bovis ; Neoplasm Metastasis ; Nevus ; Nevus, Pigmented* ; Peas ; Shoulder ; Skin ; Subcutaneous Tissue

Pagets disease is charaterized by a unilateral sharply defined eczema with or without an underlying ductal adenocarcinoma of the female nipple which was described by James Paget on 1874. We experienced 2 cases of Pagets disease confined to the nipple & areola with or without a palpable mass. The one was 44-year-old female, who had been suffered from pea. sized, eczematous lesion and crust formation on the Lt. nipple and areola with a painful palpable mass in the Lt, breast and nipple retraction since about 3years ago(Fig. 1). The another one was 52-year-old female, who had been suffered frorn palm sized, same lesions of the former on the Lt. breast with accompanying itching sensation and without a palpable mass and nipple retraction since about 7years ago(Fig. 5). Histopathologically, there were large, round, clear staining cells with Iarge nuclei in the epidersi and an inflammatory infiltration in the dermis. Diagnosis was confirmed by the clinical and histological pictures. The former was treated by simple mastectomy and the latter by radical mastectorny.
Adenocarcinoma ; Adult ; Breast ; Dermis ; Diagnosis ; Eczema ; Female ; Humans ; Mastectomy, Simple ; Middle Aged ; Nipples ; Peas ; Pruritus ; Sensation

No abstract available.
Cicatrix* ; Contracture*

No abstract available.
Peptic Ulcer* ; Retrospective Studies*

No abstract available.
Cardiopulmonary Bypass* ; Endarterectomy* ; Pulmonary Embolism*