HLA-A, B, C and HLA-DR are very important in the regulation of immune response and of transplant success or failure, but there have been a few reports about F3I A expression in melanocyt.es, a minor population of cpidermal cells. In the treatment of stable or segmental vitiligo, allogeneic melanocytes graft could t)e potentially useful if rejection does not. occur. The aim of this study was to examine the expression of HI.A-A, B, C and HI.A-DR in cultured hurnan melanocytes with and without. stimulation by INF-r and suction blister fliid. All strains of cultured me- lanocytes expressed HLA-A, B, C regardless of stmulation, but HLA-DR was expressed only following stimulation of INI-r. HLA expression in me]anocytes appears to be similar to that in keratinocytes.
Blister* ; HLA-A Antigens ; HLA-DR Antigens* ; Humans* ; Keratinocytes ; Melanocytes* ; Suction* ; Transplants ; Vitiligo

No abstract available.
Drug Eruptions*

Suction is a useful tool for separation of epidermis from dermis in vivo. With appropriate suction pressures and durations applied, cellular damage of the separated epidermis can reduce, This study was performed to examine the appropriste suction pressures and durations in the lhsional and normal skin of four vitiligo patients who wanted to take epidermal autograft. The suction pressures and durations for making the blisters of similar morphology were different according to person. Microscopic changes in the blisters of similar morphology were not very different, and suction of 200 to 250 mmHg for 2 to 3 hours with a 2cm diametered suction cup was considered to be acceptable. Obvious damage to keratinocytes and diminish of melanacyte number were observed with much more powerful and longer suction such as suction of 350 to 400 mmHg for 3 to 4 hours. The level of dermo-epidermal separation was between basal cell membrane.
Autografts ; Blister ; Cell Membrane ; Dermis* ; Epidermis* ; Humans ; Keratinocytes ; Skin ; Suction* ; Vitiligo*

Lesional and normal skins in 10 patients of fixed drug eruption were studied. Number and vertical distance of rete ridges were measured in H & E stained specimens. Amount of free melanin and number of melanocytes were counted in dopa stained epidermal sheets. According to the number of recurrence, activity and number of melanocytes see med to be changed. The lesions of a few recurrence showed only active melano cytes, however, those of frequent recurrenoe increased number of melanocytes with marked elongation of rete ridges. It is supposed that the numerical increase of melanocytes were from the elongation of rete redges.
Dihydroxyphenylalanine ; Drug Eruptions* ; Humans ; Melanins ; Melanocytes* ; Recurrence ; Skin

No abstract available.
DNA, Mitochondrial* ; Essential Tremor* ; Humans

No abstract available.
Denture, Complete* ; Finite Element Analysis*

Two cases of pseudoxanthoma elasticum associated with angioid streaks in both eyes were presented and the literatures were reviewed. The affected sisters were 15 and 18 years female among the five siblings. They had pin head sized yellowish maculopapular eruptions on the sides of the neck and axillae, and also visual distubance of the both eyes. They seemed to be autosomal recessive inberitance in their family background. There were no other complications.
Angioid Streaks ; Axilla ; Female ; Head ; Humans ; Neck ; Pseudoxanthoma Elasticum* ; Siblings

Sensitization to Cenasert®, a broad spectrum antimicrobial for the treatment of leukorrhea, is rare. Perineal contact dermatitis due to vaginal suppositories has never been reported in the medical literature. We report a 31-year-old woman, who developed perineal skin eruptions following the treatment of leukorrhea with Cenasert® vaginal suppositories. Patch tests showed positive reactions to Cenasert® suppositories and to the three active ingredients of Cenasert®. Vaginal suppositories can sensitize not only the vagina but also the surrounding perineum due to leakage of the allergen from the vaginal vault.
Adult ; Dermatitis, Allergic Contact* ; Dermatitis, Contact ; Female ; Humans ; Leukorrhea ; Patch Tests ; Perineum ; Skin ; Suppositories* ; Vagina

In 1927, Hamperl carried out histochemical studies on gastric cancer and found one case which contained many Fontana reactive argentaffin cells. Thereafter the gastric and intestinal adenocarcinomas which contain enterochromaffin cells such as argentaffin cell and argyrophil cell have been reported by several investigators. As the results, many reports revealed that 1.7 to 8% of gastric carcinomas and about 3% of intestinal carcinomas contained argentaffin cells, and 13 to 20% of gastric carcinomas and 12 to 15% of intestinal carcinomas contained argyrophil cells. The present study is aimed to evaluate the frequency of argentaffin and argyrophil cells in gastric and intestinal carcinoma in relation to the histopathological patterns. The material consisted of 282 cases of gastric adenocarcinoma and 83 cases of intestinal carcinoma which were diagnosed at the Department of Pathology, Yonsei University College of Medicine from January 1982 through December 1983. All cases were stained with hematoxylin-eosin for histological classification and with silver impregnation techniques for argentaffin and argyrophil granules. Staining for mucin was also performed using PAS stain. The results obtained were as follows: 1) Among 282 cases of gastric carcinomas, Grimelius positive argyrophil cells are noted in 43 cases (15.2%) and Fontana-Masson positive argentaffin cells are noted in 5 cases (1.8%). 2) Among 83 cases of intestinal carcinomas, Grimelius positive argyrophil cells are noted in 15 cases (18.2%) and Fontana-Masson positive argentaffin cells are noted in 1 cases (1.2%). 3) In gastric carcinoma, argyrophil cells are present in 31 cases (17.5%) among 176 male patients and and in 12 cases (11.3%) among 106 female patients. 4) In intestinal carcinoma, argyrophil cells are present in 9 cases (20.4%) amogn 44 male patients, and in 6 cases (15.4%) among 39 female patients. 5) According to classification by Willis, argyrophil cells are present in 12 cases (11.1%) among 108 well to moderately well differentiated adenocarcinoma, 14 cases (14.4%) among 99 poorly differentiated adenocarcinoma, 6 cases (30%) among 20 mucinous carcinoma, and 11 cases (21.1%) among 52 undifferentiated carcinoma. There is no significant difference in the frequency of argyrophil cells in relation to the histological type. 6) According to general classification of intestinal carcinoma, argyrophil cells are present in 9 cases (21.4%) among 42 well to moderately well differentiated adenocarcinoma, 3 cases (15%) among 25 poorly differentiated adrcinom adenocarcinoma and and cases (18.7%) among 16 mucinous carcinoma. The argentaffin cells were noted only in one case, which was the case of well to moderately well differentiated adenocarcinoma. In summary, the results of this study indicates that the frequency of enterochromaffin cells was 15.2% in stomach carcinoma and 18.2% in intestinal carcinoma. According to histological type, the Grimelius positivity was higher in mucinous and undifferentiated type, and higher frequency was noted in male than female.
Female ; Male ; Humans ; Adenocarcinoma ; Stomach Neoplasms

Aquagenic urticaria is a rare form of physical urticaria in which wheal is produced by contact with water. A 21-year-old woman was referred to our department because of itching wheal after contact with water for 1 month. Match-head-sized wheal surrounded by bean sized erythema was provoked with water on her face regardless of its tempera ture, osmolarity and quality. Passive transfer test was negative. Result of pro vocation test with normal saline and NaHCO solution, which was reported to be therapeutically effective, was similar to those with pure water.
Erythema ; Female ; Humans ; Intradermal Tests ; Occupations ; Osmolar Concentration ; Pruritus ; Urticaria* ; Water ; Young Adult