1.Effect of Cumulus Cell Coculture on Early Mouse Embryonal Development in vitro.
Jae Du YOO ; Hyun Jeong PARK ; Yu II LEE
Korean Journal of Obstetrics and Gynecology 2000;43(4):665-669
OBJECTIVE: To evaluate whether the rate of early mouse embryonal development could be enhanced by cumulus cell coculture in vitro. METHODS: Ham's F-10 culture media supplemented with 0.4% bovine serum albumin were used. Two-cell F1 mouse embryos were cultured in media with or without cumulus cells of female ICR mouse embryo for 96 hours, and the rates of embryonal development were observed and compared. RESULTS: The percentage of hatched blastocyst in the coculture group was significantly higher than that in the control group by 87.3% vs 64.8% respectively (p< 0.05). CONCLUSION: This study provides confirmative information that cumulus cell coculture will be useful in enhancing early mouse embryonal development.
Animals
;
Blastocyst
;
Coculture Techniques*
;
Culture Media
;
Cumulus Cells*
;
Embryonic Structures
;
Female
;
Humans
;
Mice*
;
Mice, Inbred ICR
;
Serum Albumin, Bovine
2.A Case of Lenear IgA Bullous Dermatosis.
Ki Beom PARK ; Kwang Hyun CHO ; Yoo Shin LEE
Korean Journal of Dermatology 1986;24(5):707-711
We report a 27-year-old male with rnultiple pruritic papulovesicular eruptions on the whole body without any gluten-sensitive enteric syrnpoms. A skin biopsy specimen showed subepidermal vesicles and papillary microabscesses infiltrated with rnany neutrophils and a few lymphocytes and eosinophils. Direct immunofluorescent study of the perilesional skin revealed linear deposits of immunoglobulin A along the dermo-epidermal junction. The lesion responded rapidly to oral dapsone and topical steroid therapy.
Adult
;
Biopsy
;
Dapsone
;
Eosinophils
;
Humans
;
Immunoglobulin A*
;
Linear IgA Bullous Dermatosis
;
Lymphocytes
;
Male
;
Neutrophils
;
Skin
;
Skin Diseases*
3.The change of head posture after orthognathic surgery in mandibular prognathism.
Jung Hyun PARK ; Ji Ho YOO ; Choung Kook YI
Journal of the Korean Association of Oral and Maxillofacial Surgeons 1993;19(4):429-444
No abstract available.
Head*
;
Orthognathic Surgery*
;
Posture*
;
Prognathism*
4.Morphea on the Face in a Patient with Rheumatoid Arthritis.
Seong Kyu KIM ; Chan Kum PARK ; Dae Hyun YOO
The Journal of the Korean Rheumatism Association 2006;13(1):91-92
No abstract available.
Arthritis, Rheumatoid*
;
Humans
;
Scleroderma, Localized*
5.The Distribution of HLA Antigens and Haplotypes in Koreans.
Hyun Soo KIM ; Yoo Sung HWANG ; Myoung Hee PARK
Korean Journal of Clinical Pathology 1997;17(6):1109-1123
BACKGROUND: The HLA system is known to be the most polymorphic genetic system in human and there are characteristic racial differences in the distribution of HLA antigens, alleles, and haplotypes. This study was performed to examine the frequency of HLA antigens, alleles and haplotypes in Koreans. METHODS: Two thousand healthy Koreans registered for unrelated bone marrow donors were subject to the study. HLA-A, B and C antigens were typed by the serological method, and HLA-DR DNA typing (low resolution) was done by PCR and reverse hybridization. HLA allele and haplotype frequencies and linkage disequilibrium values were calculated by the maximun likelihood method using the computer program of the 11th International Histocompatibility Workshop. RESULTS: HLA antigens identified in 2000 Koreans were 14 in A locus, 33 in B locus, 8 in C locus and 12 in DR locus. Alleles showing frequencies of more than 10% in decreasing order of frequency In each HLA locus were A2, A24, A33, All, B44, B62; CBL, Cw3, Cwl, Cw7, DR4, DR2, DRl3, DR8, and DR9. Among A-B, C-B, B-DR 2-locus haplotypes, A33-B44, A30-B13, Al-B37, Cwl-B54, Cw4-B62, B7-DR1, B37-DR10 showed strong positive linkage disequilibrium (Chi-square > 1000). The most common A-B-DR haplotypes in Koreans occurring at frequency of more than 2% were A33-B44-DRl3 (4.8%), A33-B58-DRl3 (3.2%), A33-B44-DR7 (2.6%), All-B62-DR4 (2.3%), A24-B7-DR1 (2.3%), and A30-Bl3-DR7 (2.1%) Comparison of the distribution of A-B-DR haplotypes among east Asian populations reveals that Koreans are most close to Japanese, but show higher degree of polymorphism in the distribution of HLA haplotypes compared to Japanese. CONCLUSIONS: The results obtained in this study can be used as basic data for Koreans in the fields of organ transplantation, disease association studies and anthropologic studies.
Alleles
;
Asian Continental Ancestry Group
;
Bone Marrow
;
DNA Fingerprinting
;
Education
;
Haplotypes*
;
Histocompatibility
;
HLA Antigens*
;
HLA-A Antigens
;
HLA-DR Antigens
;
Humans
;
Linkage Disequilibrium
;
Organ Transplantation
;
Polymerase Chain Reaction
;
Tissue Donors
;
Transplants
6.Effects of Capsaicin Pretreatment on Lung Colonization of B16 Melanoma and mRNA Expressions for Some Cytokines in Mice.
Hyun Joo HA ; Jae Seung PARK ; Dae Yoo HA
Korean Journal of Immunology 2000;22(3):123-130
No abstract available.
Animals
;
Capsaicin*
;
Colon*
;
Cytokines*
;
Lung*
;
Melanoma, Experimental*
;
Mice*
;
RNA, Messenger*
7.Basal Cell Carcinoma with Metaplastic Bone Formation
Dae San YOO ; Seh Hyun PARK ; Mi Ryung ROH
Korean Journal of Dermatology 2019;57(5):279-280
No abstract available.
Carcinoma, Basal Cell
;
Osteogenesis
8.A Case of Bullous Lichen Planus.
Heung Bae PARK ; Yoo Chan KIM ; Kwang Hyun CHO ; Yoo Shin LEE
Korean Journal of Dermatology 1986;24(6):832-836
We herein present a case of bullous lichen planus in a 56-year-old female. She had had a generalized eruption of lichen planus with violaceus papules and plaques. many of which had been surmounted by vesicles and bullae. The histopathologic findings of a bulla revealed hyperkeratosis, irregular acanthosis, subepidermal bulla and upper dermal band-like cell infiltration. The patient had been treated with dapsone but the lesions had not been significantly improved.
Dapsone
;
Female
;
Humans
;
Lichen Planus*
;
Lichens*
;
Middle Aged
9.Identification of Differentially Expressed Genes in Human Fetal Astrocytes.
Hye Myung YOO ; Hyun Sook PARK ; Hyun Chul CHO ; Choon Myung KOH ; Joo Young PARK
Journal of the Korean Society for Microbiology 2000;35(5):374-374
No Abstract Available.
Astrocytes*
;
Humans*
10.Development of the Health-Information System using a Multimedia Tool.
Sun Mi YOO ; Yoo Seock JEONG ; Eal Whan PARK ; Sang Hoon LEE ; Sun Ryoung PARK ; Ji Hyun SUH
Journal of the Korean Academy of Family Medicine 1997;18(6):645-656
BACKGROUND: As people have been interested in healt,h and as the microcomputers with multimedia steeing have been supplied more and more, there is increasing need of multimedia information about health. The CD-ROM, which is a representative multimedia tool, is capable of interacting between information-provider and users, offering audiovisual interface, and saving great deal of knowledge. METHODS: We developed the health-information system to provide ordinary people with hea1th information using multimedia tools. It, has 7 parts ; self-diagnosis, exploring human body, drug information, hospital information, first-aid treatment, Q and A, and medical common sense. We got image data such as figures, photos, X-rays, electrocardiograms and pathologic specimens with scanner and film scanner, also moving image(video) with video-capturing program. RESULTS & CONCLUSIONS: We hope the multimedia health-information system to become a useful methodology of patient education and to be used as an information source in medical information network in the near future.
CD-ROM
;
Electrocardiography
;
Hope
;
Human Body
;
Information Services
;
Microcomputers
;
Multimedia*
;
Patient Education as Topic