1.Establishment of early warning control charts based on the syndromic surveillance data of outpatient diarrhea in Beijing
Pinze WANG ; Min LIU ; Xiaoqiu DAI ; Xuesong YANG ; Xiaoguang LI ; Li SHEN ; Zhenghui WANG ; Hua WU ; Jie XU ; Yongzhong NING
Journal of Peking University(Health Sciences) 2014;(3):424-428
Objective:To establish the control charts for early warning of diarrhea based on the syn-dromic surveillance data from enteric clinic in Beijing .Methods:The outpatient data from enteric clinic of a Grade Three General hospital in Haidian district , Beijing from April 1 to Oct.31, 2009 and from May 1 to Nov.10, 2010 were collected, according to the moving average method , the baseline calcula-ted, the value of probability αand μα, the early warning value based on the formula “w=Xj +μαSj”calculated and the early warning control charts drew at last .Results:According to the harmfulness , the severity and controllability of diarrheal diseases , the value of probability αwas determined as 0.01, thenμα( unilateral) as 2, based on the early warning value , the control charts of diarrheal diseases , bacillary dysentery and other infectious diarrhea were established .Conclusion:The enteric clinic requires to fur-ther collect baseline data to evaluate and continuously adjust the established control charts for the best early warning model in accordance with the enteric clinic .
2.The effect of shRNA targeting hTERT on telomerase and the expression of PCNA and Caspase-3 in nasopharyngeal carcinoma cells.
Yongzhong SHEN ; Yan WANG ; Shiming CHEN ; Bokui XIAO ; Jun SU ; Zezhang TAO
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2008;22(9):411-415
OBJECTIVE:
Analysis of the correlation between telomerase and expression of its related proteins may provide insight into the molecular mechanism of nasopharyngeal carcinogenesis. Here, we investigate the effect of short hairpin RNA (shRNA) specific for human telomerase reverse transcriptase (hTERT) mRNA on the expression of proliferating cell nuclear antigen (PCNA) and Caspase-3 in nasopharyngeal carcinoma (CNE) cells.
METHOD:
shRNA expression vectors targeting the mRNA of hTERT were constructed. Cells were treated with the constructed expression vectors and telomerase activity was measured by telomeric repeat amplification ELISA (TRAP-ELISA). Cell viability was examined using the MTT assay. Cell apoptosis was detected by TUNEL method. The expression of PCNA and Caspase-3 proteins, was determined by Western blotting.
RESULT:
shRNA specific for hTERT mRNA significantly inhibited telomerase activity, suppressed cell viability and induced apoptosis in CNE cells. In addition, the expression of PCNA was inhibited, while the expression of Caspase-3 was up-regulated.
CONCLUSION
Our results suggest that shRNA directed against hTERT inhibits cell viability by regulating telomerase activity and its related protein expression in NPC cells. Therefore, RNA-interfering technology may be a promising strategy for the treatment of nasopharyngeal cancer.
Caspase 3
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metabolism
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Cell Line, Tumor
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Cell Survival
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Humans
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Nasopharyngeal Neoplasms
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genetics
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metabolism
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Proliferating Cell Nuclear Antigen
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metabolism
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RNA, Small Interfering
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Telomerase
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genetics
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Transfection