Objective To clone and sequence variant-specific surface antigen gene from Giardia lamblia isolate SUCH/89/BTMRI/2(C2) derived from human in China. Methods Total genomic DNA of G.lamblia was extracted and a full-length variant-specific surface antigen gene fragment was amplified by pelymerase chain reaction (PCR). The PCR product was cloned into pMD19-T simple-vector, transformed into an Escherichia coli JM109 strain and then sequenced. The sequence analysis for cloned fragment was finished by Vector NTI 9.0 software for the homology of Giardia variantspecific surface antigen gene to that of sequences publishend in GenBank. Results The full-length variant-specific surface antigen gone fragment from G. lamblia was found to be 2 142 bp, encoding a 713 amino acid polypeptide and contained a single open reading frame (ORF). The deduced polypeptide sequence was rich in cysteine (11.8 mol%), most of which occurred with in 29 copies of the 4-amino acid CXXC motif, one GGCY-tetrapeptide motifs and three NXS consensus N-linked glycosylation sites. This polypeptide was also rich in threonine (10.2 mol%), glycine (12.1 mol%) and alanine (10.1 mol%). Like other previously identified VSPs, it contained a highly conserved hydropbebic Cterminal region. The homology of G. lamblia SUCH/89/BTMRI/2(C2) variant-specific surface antigen gene to that of sequence (TSA417) published in GenBank was 99% both at the nueleotide and the amino acid levels. Conclusion The full length variant-specific surface antigen gene from the isolate of G. lamblia has the common characteristics with other previously identified VSPs.

Objective To analyze the employment situation of graduates majoring in nutrition and provide employment guidance and reference for them. Methods Materials about 172 graduates of 2013 to 2015 were collected from relevant departments. The software of Excel was used for data processing and SPSS 19.0 was used for data statistics. The crosstabs with chi-square was used for statistic test. Results The overall employment rate was 66.3% (114/172), which was 82.9% (58/70), 56.9% (33/58) and 52.3% (23/44) respectively from 2013 to 2015. There was no statistical difference among different genders ( χ2=1.05, P=0.30) and residence registration types ( χ2=1.06, P=0.30). Graduates who are a member of the Communist Party got a statistical higher employment rate than those who are not (χ2=8.75, P=0.01). Most graduates chose to work in the medical units or to further their study with the proportion of 47.4% (54/114) and 26.3% (30/114) respectively This was especially common for the 2015 graduates. 60.9% of them chose to work in the medical units. And those who chose to work in the urban areas of the city accounted for 64.9%. Conclusion Compared with the employment rates of other majors in the same medical university, the rate of the nutrition graduates was lower. The concerted efforts are needed from the society, the university and the graduates themselves.