1.Influence of Post-stroke Depression on Early Restore
Ruihua MA ; Yongzhen LIU ; Jie LONG
Chinese Mental Health Journal 1988;0(06):-
Objective: To study the influence of PSD (post-stroke depression) on early restore Method: 67 patients with acute stroke were evaluated with HAMD and NFA (neurological function assessment) They were followed for 6 months after discharge Results:The rate of PSD was 37%, most in mild to moderate levels Patients with PSD stayed longer in hospital than those without In follow up, patients with PSD had poor outcome in NFA Conclusion: PSD has negative influence on restore of stroke
2.Effects of transplanting bone marrow cells on cardiomyocyte apoptosis and expressions of Bcl-2 and Bax in post-infarction rats
Dongxing MA ; Yongzhen ZHANG ; Daoyin XIE
Chinese Journal of Interventional Cardiology 2003;0(05):-
Objective To clarify the role of cardiomyocyte apoptosis in cardiac function after transplantation of bone marrow cells. Methods A mycardial infarction model was induced in SD rats by left anterior descending artery ligation. 5?106 of bone marrow mononuclear cells were injected into peri-infarct zone (BMT group). In 4 or 8 weeks post-infarct, apoptosis of cardiomyocytes and expression of Bcl-2/Bax protein were determined by TUNEL or immunohistochemistry. Cardiac function was evaluated by echocardiography (UCG). Results TUNEL results indicated that apoptosis index of peri-infarct zone from the BMT group was reduced significantly when compared with the control group (4 wk: 0.094?6?0.017 vs 0.173?0.018, P
3.Effects of delayed transplantation of bone marrow cells on the expressions of bcl-2/bax mRNA and proteins in myocardial cell in rat
Dongxing MA ; Huiliang LIU ; Yongzhen ZHANG
Medical Journal of Chinese People's Liberation Army 2001;0(08):-
Objective To evaluate the effects of delayed transplantation of bone marrow mononuclear cells (BM-MNCs) after myocardial infarction (MI) on the expressions of bcl-2/bax mRNA and proteins in myocardial cells, and to explore the possible mechanism of cardiomyocyte apoptosis. Methods The MI rat model was reproduced by ligating the left anterior descending coronary artery. Two weeks later 5?106 of BM-MNCs were injected into the infarct zone and the peri-infarct zone (BMT group). TUNEL was used to determine the cardiomyocyte apoptosis, immunohistochemical method was employed to detect the expressions of bcl-2 and bax protein, and the technique of hybridization in situ was applied to assess the changes in of bcl-2/bax mRNA expression. Results TUNEL results indicated that apoptosis index of BMT group was lowered significantly compared with the control group (4 weeks: 0.095?0.017 vs 0.173?0.018; 8 weeks: 0.0916?0.014 vs 0.182?0.015, P
4.Correlation between single nucleotide polymorphism of adiponectin gene and type 2 diabetes in Chinese
Hui XIA ; Yongzhen MO ; Rongwen BIAN ; Jie SHEN ; Lijun MA
Chinese Journal of Endocrinology and Metabolism 1986;0(03):-
Two single nucleotide polymorphisms (SNP) of adiponectin gene (SNP+45 and SNP+276) were screened in Chinese population. The results showed that the distribution of SNP+45 genotypes were significantly different and G/G genotype in type 2 diabetic population appeared more frequently than that in non-diabetic population. It suggests that adiponectin gene seems to be the susceptive gene in Chinese type 2 diabetics.
5.DNA immunity by recombinant encoding prME protein derived from Japanese encephalitis virus and immunogiobulin G Fc of BALB/c mice
Ximei LI ; Yan ZHOU ; Yongzhen ZHAI ; Li MA ; Guohe FENG
Chinese Journal of Microbiology and Immunology 2008;28(7):634-638
Objective To study the effect of lgG Fc gene on JEV DNA vaccine immunity. Methods Gene encoding IgG Fc was amplified by nested-RT-PCR technique from BALB/c murine spleen cells. JEV prME protein gene was obtained with restriction endonuclease BamH Ⅰ/EcoR Ⅰ from the eukaryotic recombinant named after pJME, which was constructed by us before. Recombinant, named after pJME/IgG Fc, with above two genes encoding JEV prME protein and BALB/c murine IgG Fc was constructed, and was tested by restriction enzymes analysis and DNA sequencing, then was transfected into China hamster ovary (CHO) cells by Lipo-fectAMINE 2000. Distribution and expression of the fusion proteins encoded by JEV prME protein and BALB/c murine IgG Fc genes in transfected CHO cells were detected by immunofluorescence and Western blot. The BALB/c micc were vaccinated with pJME/IgG Fc via intramuscular injection. Then the cytotoxic T lymphocyt (CTL) activity were assessed by lactic dehydrogenase (LDH) and the neutralizing antibody titer were assessed by 80% plaque reduction neutralization test. Results Molecular weights (2001 bp, 2730 bp) of the two in- serts released from pJME/IgG Fc with two group of restriction analysis associated with BamH 1/EcoR I and BamH Ⅰ/Not Ⅰ were correlated to the expected theoretic results respectively. It was estimated that molecular weight (Mr) of the fusion protein was 101 x 103. The expression of the above fusion protein was mainly distribu- ted in endochylema of transfected CHO cells,and not much in membrane of transfected CHO cells. CHO cells transfected with pJME/IgG Fc could express the fusion protein at the 32th cell passage. After immunization, the CTL activity and the neutralizing antibody titer in the pJMF/IgG Fc vaccinated group increased significantly compared with other vaccinated groups(P <0.05). Conclusion The recombinant pJME/IgG Fc was construc- ted and transfected into CHO cells successfully, and CHO cellular lines expressed fusion protein encoded by JEV prME protein and BALB/c murine lgG Fc genes stably were obtained. IgG Fc gene could reinforce the cellular immunity and humoral immunity of JEV DNA vaccine.
6.Construction and Expression of Recombinant Plasmid Encoding prME Protein Derived from Japanese Encephalitis Virus and Granulocyte-macrophage Colony-stimulating Factor of BALB/c Murine
Yongzhen ZHAI ; Yan ZHOU ; Li MA ; Guohe FENG
Journal of China Medical University 2010;(9):706-709
Objective Construction and expression of recombinant plasmid fusing encoding prME protein derived from Japanese encephalitis virus (JEV) and GM-CSF of BALB/c mouse. Methods GM-CSF gene was ampli/ied by nested-RT-PCR from BALB/c spleen cells. JEV prME protein gene was eluted by the digestion with restrict/on endonucleases BamH I/EcoR I from pJME piasmid. Genetic fusion of prME protein and GM-CSF are subcloned info pcDNA3.1 (+) eukaryotic vector, and named as pJME/GM-CSF.The recombinant was confirmed by restriction enzymes digestion and DNA sequencing, and then transfected into China hamster ovary (CHO) cells by Lipofectamine2000. pJME/ GM-CSF expression in CHO cells was examined by Western blot. Results We observed 2001 bp and 2472 bp DNA fragments when pJME/GM-CSF was digested with BamHI/EcoRI and BamHI/NotI respectively as expected. The estimated molecular weight of the fusion protein was 85kD. Conclusion The recombinant pJME/GM-CSF was constructed and transfected into CHO cells successfully with pJME/GMCSF stably expressed.
7.Expression of TAK1, pTAK1 proteins and density of tumor associated macrophages in pancreatic carcinoma
Dan MA ; Fangfang CHENG ; Fan YANG ; Xiaohua MAN ; Jun GAO ; Yongzhen ZHANG ; Zhaoshen LI
Chinese Journal of Pancreatology 2015;15(3):169-172
Objective To investigate the expression of TGF-β activated kinase1 (TAK1),phosphoryted TAK1 (p-TAK1) and the density of tumor associated macrophages (TAM) in human pancreatic carcinoma tissues and pairing adjacent normal pancreatic tissues,and explore their relationship.Methods The expression of TAK1,p-TAK1,CD68 (the marker of tumor associated macrophages) proteins in 57 samples of pancreatic cancer tissues and 35 samples of pairing adjacent normal pancreatic tissues were detected by immunohistochemical method,then the density of TAM was evaluated.The relationship between the protein expression and TAM,and the relationship between them and clinicopathologic parameters were examined using SPSS 18.0 software,and the independent risk factors of TNM staging were analyzed by multivariate logistic regression.Results TAK1 and p-TAK1 were positively expressed in 42.1% (24/57) and 40.4% (23/57) pancreatic carcinoma tissues,significantly higher than adjacent normal pancreatic tissues [14.3 % (5/35) and 11.4% (4/35)];the proportion of pancreatic carcinoma tissues with high density TAM was 38.6% (22/57),higher than that of adjacent pancreatic tissues [8.6% (3/35)],the differences were statistically significant (P<0.05).TAK1 expression was positively related to tumor size,tumor differentiation,lymph node metastasis,distant metastasis and clinical staging;p-TAK1 expression was positively related to tumor differentiation,lymph node metastasis,distant metastasis and clinical staging;the density of TAM was positively related tumor differentiation,lymph node metastasis,distant metastasis and clinical staging (all the P values were less than 0.05).The expression of TAK1 and p-TAK1were positively correlated with the density of TAM (P <0.001).Multivariate logistic regression analysis showed that high density of TAM was independently associated with advanced clinical staging (P =0.002,OR =129.5,95% CI 6.2 ~2718.6).Conclusions TAK1 pathway and TAM may play an important role in the pathogenesis and progression of pancreatic cancer,and there may be synergy effect between them.
8.Incidence analysis of malignant cancer in Shanxi cancer registration areas in 2011
Yongzhen ZHANG ; Ling CAO ; Zhaohui MA ; Fang SU ; Yi XU ; Yuan WANG ; Ruifeng ZHANG ; Xinchen WANG
Cancer Research and Clinic 2016;28(7):471-475
Objective To explore the cancer incidence in registration areas in Shanxi Province. Methods Data of 8 cancer registration areas in 2011 were taken into account and cancer incidence in different areas with different ages was compared with that in other domestic areas. Results 8 395 new cases in Shanxi all cancer sites were reported in 2011, including 4 810 male and 3 585 female. The incidence of malignant cancer of Shanxi was 207.53/100 000, and the standardized incidence of Chinese population and world population were 125.20/100 000 and 165.72/100 000, respectively. In urban areas, the incidence of Shanxi and the standardized incidence of Chinese population were 202.49/100 000 and 112.81/100 000, respectively. In rural areas, incidence rate of Shanxi was 211.96/100 000 and the standardized incidence of Chinese population was 138.43/100 000. In Shanxi Province, the major malignant cancer sites for males involved stomach, lung, esophagus, liver and colorectum, and cancer sites for females were more on cervix, lung, breast, stomach and esophagus. Conclusions Upper gastrointestinal cancer and uterine cervix cancer are the major cancers in Shanxi registration areas. The incidence of stomach cancer and uterine cervix cancer in Shanxi Province are much higher than national average.
9.Tissue-engineered skin construction with amniotic epithelial cells and amniotic mesenchymal stem cells
Biao XU ; Fang LI ; Qing SUN ; Yunyun XU ; Juan ZHAO ; Hansi LIANG ; Shuli MA ; Yongzhen CHEN
Chinese Journal of Tissue Engineering Research 2013;(41):7213-7220
BACKGROUND: Placenta mesenchymal stem cells have become hot spots in stem cells study in recent years because of its advantages. OBJECTIVE:To analyze the biological characteristics of amniotic mesenchymal stem cells and amnion epithelial cells, and to explore the feasibility of amniotic mesenchymal stem cells and amnion epithelial cells applied as seed cells in three-dimensional liquid culture to construct the tissue-engineered skin. METHODS:The amniotic mesenchymal stem cells and amnion epithelial cells were obtained by using multi-step digestion with trypsin and col agenase;then the flow cytometry, reverse transcription-PCR and immunofluorescent staining techniques were adopted to identify the surface molecular markers, stem cellcharacteristics and keratinocytes similarity respectively. Based on these data, amniotic mesenchymal stem cells and amnion epithelial cells used as seed cells together with rat type Ⅰ col agen matrix were adopted for three-dimensional liquid culture. RESULTS AND CONCLUSION:Flow cytometry test showed that amniotic mesenchymal stem cells and amnion epithelial cells cultured in vitro could highly express CD90, CD73 and CD105, and could not express the hemopoietic stem cellmarker of CD34 and MHC-class Ⅱ molecular HLA-DR. Reverse transcription-PCR results detected that amniotic mesenchymal stem cells could express stem cellcharacteristic genes CMCY and NANOG;amnion epithelial cells could express stem cellcharacteristic genes CMCY and KLF4, showing that both amniotic mesenchymal stem cells and amnion epithelial cells have stem cellproperties. Reverse transcription-PCR results showed that amniotic mesenchymal stem cells could express keratinocytes characteristic genes K19,β1-integrin and K8;amnion epithelial cells could express K19,β1-integrin, K5 and K8. Immunofluorescence staining results showed amnion epithelial cells could express keratinocytes proliferation related protein K14, which revealed that there was certain similarity in the mRNA expression between keratinocytes and amnion epithelial cells, and indicating that it has the potential to differentiate into keratinocytes. Tissue-engineered skin was successful y constructed by using amniotic mesenchymal stem cells and amnion epithelial cells, hematoxylin-eosin staining section showed that it has certain skin structure, and amnion epithelial cells had a preliminary differentiation. Al these prove that it is feasible to construct human skin tissues with amniotic mesenchymal stem cells and amniotic epithelial cells through the three-dimensional culture.
10.Application of human papillomavirus 16/18 E6 protein detection for cervical cancer screening
Yu ZHANG ; Lixin SUN ; Hongwei ZHAO ; Yongzhen ZHANG ; Lixia WANG ; Zhaohui MA ; Yi XU ; Fang SU ; Ruifeng ZHANG
Cancer Research and Clinic 2017;29(3):164-167
Objective To evaluate the diagnostic accuracy and efficacy on cervical precancerous lesions and cervical cancer by HPV 16/18 E6 protein detection. Methods A total of 439 females with sexual activities were selected from Department of Gynaecology in Shanxi Cancer Hospital from May 2014 to January 2015, including 299 cases of cervical intraepithelial neoplasia (CIN)Ⅱ, CINⅢ or cervical cancer (the case group), and the other 140 cases (the control group). All the patients accepted the thinprep cytology test (TCT), HPV DNA and HPV 16/18 E6 oncoprotein tests and colposcope examination. Results The positive rates of the TCT, HPV DNA, HPV 16/18 E6 oncoprotein in the case group were 97.0 % (290/299), 94.3 % (282/299) and 66.9 % (200/299), respectively, and those in the control group were 44.3 % (62/140), 21.4 % (30/140) and 2.9%(4/140), respectively, and there were significant differences between both groups (all P<0.05). The sensitivity and specificity of the HPV 16/18 E6 oncoprotein test in detecting CINⅡ and above were 66.9 %and 97.1 %, respectively, and both of HPV DNA test were 94.3 % and 78.6 %, respectively; The consistent rate between HPV 16/18 E6 and HPV DNA was 71.9 % (κ= 0.21). In the case group, when TCT was associated with HPV DNA test, the sensitivity, specificity and accuracy were 98.9%, 82.8%and 81.7%, respectively, and when TCT was combined with HPV 16/18 E6 oncoprotein test, those were up to 97.9 %, 97.1 % and 95.0 %. Conclusion HPV 16/18 E6 oncoprotein test can improve the specificity of cervical cancer screening, so it may be used as a primary screening method in the less developed areas where HPV DNA test is difficult to be carried out, or as a shunt method for HPV DNA positive patients, which will allocate the limited health resources rationally.