Objective To explore the differentially expressed genes in peripheral blood mononuclear cells of patients with primary biliary cirrhosis and compare it with healthy controls.Methods Peripheral blood mononuclear cells were isolated from 9 primary biliary cirrhosis patients and 9 age and sex matched healthy controls.Total RNA was extracted from peripheral blood mononuclear cells and analyzed by human genome oligonucleotide microarrays (22K).The differences of gene expression and signaling pathway of patients and healthy controls were compared.Results Seventy-nine genes differentially expressed in primary biliary cirrhosis were identified by microarray analysis,in which 21 were up-regulated and 58 were downregulated.The genes were further categorized into 27 signaling pathways,in which 6 pathways were involved in immune regulation and apoptosis:natural killer cell mediated cytotoxicity pathway,toll-like receptor signaling pathway,antigen processing and presentation pathway,cytokine-cytokine receptor interaction pathway,T cell receptor signaling pathway and apoptosis pathway.Conclusion This study has identified that some genes are different in transcription expression in the primary biliary cirrhosis patients.It may provide new clues for the pathogenesis and biomarker studies.

Objective To explore the effect of hydrocortisone sodium succinate on perioperative airway management in bronchial asthma patients. Methods 47 perioperative bronchial asthma patients with artificial airway in Linzhou City People Hospital were enrolled,and they were randomly divided into control group(24 cases) and therapy group(23 cases). Doxofylline 300 mg intravenous(IV)drip per day was given to the patients in both groups,and in therapy group,additionally hydrocortisone sodium succinate 500 mg IV drip per day was applied. The remission of asthma and changes in vital signs,arterial blood gas and respiratory function were observed in both groups. Results There were no significant differences in remission rate and invalid number at 30 minutes after treatment between therapy group and control group(both P>0.05). The remission rate of asthma in therapy group at 1 hour after treatment was significantly higher than that in control group(95.7%vs. 66.7%,P=0.023),the mean remission time was shorter than that in control group(minutes:38.09±15.93 vs. 45.83±18.75,P=0.012),the respiratory rate was lower(beats/min:20.8±2.3 vs. 22.3±3.3,P=0.042),and arterial partial pressure of oxygen〔PaO2(mmHg,1 mmHg=0.133 kPa):83.5±8.9 vs. 77.9±7.4,P=0.028〕,lactate〔Lac(mmol/L):1.87±0.29 vs. 2.09±0.33,P=0.029〕,forced vital capacity〔FVC(L):3.84±0.23 vs. 3.65±0.31,P=0.004〕and forced expiratory volume in 1 second〔FEV1(L):3.34±0.20 vs. 3.16±0.29,P=0.003〕were significantly increased compared with those in control group. But there were no significant differences in heart rate(HR),arterial oxygen saturation(SaO2),pH value,arterial partial pressure of carbon dioxide(PaCO2),FEV1/FVC,and peak expiratory flow (PEF)between the two groups(all P>0.05). Conclusion Hydrocortisone sodium succinate in conjunction with doxofylline can relax the symptom of perioperative bronchial asthma patients with artificial airway faster.

Objective To describe the immunological characteristics of refractory primary biliary cirrhosis compared with the typical patients for more than 1 year's administration of UDCA.Methods Sixty patients treated with UDCA for more than 1 year in our clinic were enrolled into this study.According to the response to UDCA by Paris criteria,patients were divided into refractory group (23 patients) and typical groups (37 patients).The recent peripheral lymphocyte subsets and cytokines of the two groups were tested and analyzed.One-way ANOVA and t test were used for statistical analysis.Results ① One-year treatment after diagnosis,there were no differences between the two groups in the distribution of peripheral lymphocytic subsets,meanwhile,the two groups had higher percentage of B cells,CD4+T cells,CD4+CD28+T cells and CD8+ CD28-T cells than healthy controls respectively.② The serum levels of IL-6 [(0.8±0.9) pg/ml vs (0.3±0.4) pg/ml] and HGF were higher in the refractory group than other groups.Conclusion During the plateau phase,refractory PBC patients have higher serum levels of IL-6 and HGF,which probably suggest that the refractory PBC patients may have severe immunologic disturbance in vivo.

Objective To investigate the effects of eicosapentaenoic acid (EPA) or EPA plus carboplatin on the proliferation and apoptosis of human lung cancer cell line A-549.Methods A-549 cells were cultured by 100 μg/ml carboplatin,80 μg/ml EPA,and their combination for 48 hours.MTT assay was used to determine the effect of EPA,carboplatin,or their combination on the proliferation of human lung cancer cell line A-549.The morphological changes of human lung cancer cell line A-549 were observed using HE staining,and apoptosis of A-549 cells was analyzed by flow cytometry.Results MTT assay showed that the proliferation inhibition rate of A-549 cells cultured with EPA plus carboplatin was 85.20％ ± 5.00％,which was significantly higher than that of cells cultured with 80 μg/ml EPA (32.85％ ± 3.00％,P =0.0001 ) or 100 μg/ml carboplatin (53.25％ ±3.00％,P =0.0013 ).HE staining showed apoptosis in all three groups,whereas the apoptosis rate reached 17.05％ ± 4.00％ in the combination group,which was significantly higher than that in carboplatin group (9.49％ ± 1.00％,P =0.0252).Conclusion EPA may enhance the effects of carboplatin in inhibiting the proliferation and promoting the apoptosis of human lung cancer cell line A-549.

Objective:To establish autoverification rules for coagulation tests in multicenter cooperative units, in order to reduce workload for manual review of suspected results and shorten turnaround time (TAT) of test reports, while ensure the accuracy of results.Methods:A total of 14 394 blood samples were collected from fourteen hospitals during December 2019 to March 2020. These samples included: Rules Establishment Group 11 230 cases, including 1 182 cases for Delta check rules; Rules Validation Group 3 164 cases, including 487cases for Delta check; Clinical Application Trial Group 77 269 cases. Samples were analyzed for coagulation tests using Sysmex CS series automatic coagulation analyzers, and the clinical information, instrument parameters, test results, clinical diagnosis, medication history of anticoagulant and other relative results such as HCT, TG, TBIL, DBIL were summarized; on the basis of historical data, the 2.5 and 97.5 percentile of all data arranged from low to high were initially accumulated; on the basis of clinical suggestions, critical values and specific drug use as well as relative guidelines, autoverification rules and limits were established.The rules were then input into middleware, in which Stage I/Stage II validation was done. Positive coincidence, negative coincidence, false negative, false positive, autoverification pass rate, passing accuracy (coincidence of autoverification and manual verification) were calculated. Autoverification rules underwent trial application in coagulation results reports.Results:(1) The autoverification algorisms involve 33 rules regarding PT/INR, APTT, FBG, D-dimer, FDP,Delta check, reaction curve and sample abnormalities; (2)Autoverification Establishment Group showed autoverification pass rate was 68.42% (7 684/11 230), the false negative rate was 0%(0/11230), coincidence of autoverification and manual verification was 98.51%(11 063/11 230), in which positive coincidence and negative coincidence were respectively 30.09% (3 379/11 230) and 68.42%(7 684/11 230); Autoverification Validation Group showed autoverification pass rate was 60.37%(1 910/3 164), the false negative rate was 0%(0/11 230), coincidence of autoverification and manual verification was 97.79%(3 094/3 164), in which positive coincidence and negative coincidence were respectively 37.42%(1 184/3 164) and 60.37%(1 910/3 164); (3) Trialed implementation of these autoverification rules on 77 269 coagulation samples showed that the average TAT shortened by 8.5 min-83.1 min.Conclusions:This study established 33 autoverification rules in coagulation tests. Validation showedthese rules could ensure test quality while shortening TAT and lighten manual workload.

OBJECTIVE To study the effects of Phellodendron amurense polysaccharides （PAP） on improving gouty nephropathy （GN） in rats， and to investigate its mechanism primarily by interfering the p38 mitogen-activated protein kinase （p38 MAPK）/nuclear factor-κB（NF-κB）/tumor necrosis factor-α（TNF-α）. METHODS Sixty rats were randomly divided into normal group （water）， model group （water）， allopurinol group （positive control， 20 mg/kg）， PAP high-dose， medium-dose and low-dose groups （100， 50， 25 mg/kg， by raw material） after being stratified by body weight， with 10 rats in each group. Except for the normal group， the other groups were induced to construct GN model by giving 1 500 mg/kg potassium oxazinate and 100 mg/kg adenine intragastrically for 14 days. After modeling， the rats in each group were given relevant medicine/water intragastrically， once a day， for consecutive 28 days. After the last medication， the levels of biochemical parameters related to renal function [uric acid， creatinine （Cr）， blood urea nitrogen （BUN）， xanthine oxidase （XOD）] were detected in rats， and the histopathological changes in the rat kidney were observed. The protein expressions of monocyte chemoattractant protein-1（MCP-1），TNF-α and interleukin-6（IL-6） as well as the phosphorylation levels of p38 MAPK and NF-κB p65 protein were determined in renal tissue of rats. RESULTS Compared with the normal group， the model group suffered from the dilatation of renal tubules， structural damage to glomeruli， accompanied by inflammatory infiltration and fibrosis； the contents of uric acid， Cr， BUN and XOD， the protein expressions of MCP-1，TNF-α and IL-6 and the phosphorylation levels of p38 MAPK and NF-κB p65 protein were all increased significantly （P＜0.05 or P＜0.01）. Compared with the model group， the pathological symptoms of renal tissue in rats had been improved to varying degrees in different dose groups of PAP； the contents of uric acid， Cr， BUN and XOD， protein expressions of MCP-1， TNF-α and IL-6， the phosphorylation levels of p38 MAPK and NF-κB p65 protein in PAP high-dose and PAP medium-dose groups were all decreased significantly （P＜0.05 or P＜0.01）. CONCLUSIONS PAP exhibits an anti-GN effect， the mechanism of which may be associated with inhibiting the p38 MAPK/NF-κB/TNF-α signaling pathway.