Objective To explore the treatment effect and application value of sodium hyaluronate joint celecoxib in the treatment of senile knee osteoarthritis.Methods According to the digital table,120 elderly patients with knee osteoarthritis were randomly divided into observation group and control group.The control group received injection of triamcinolone acetonide knee joint cavity, the observation group used the knee joint cavity injection of sodium hyaluronate and oral celecoxib capsule treatment.The clinical curative effect was observed in the two groups.Results The total effective rate of the observation group was 95.00%,which of the control group was 76.67%, the difference between the two groups was statistically significant(x2=8.292,P<0.05).After treatment, the nitric oxide concentration, induction type concentration of nitric oxide synthase of the observation group were (60.11±13.42)μmol/L, (15.45±4.42)U/mL, which of the control group were (93.45 ±20.46)μmol/L,(23.52±7.76)U/mL, the differences between the two groups were statistically significant(t=10.554,6.999,all P<0.05).After treatment, the Lequesne score and VAS score of the observation group were (7.25±2.08)points, (2.86±0.95)points,which of the control group were (9.89±3.15)points, (4.21±1.47) points, the differences between the two groups were statistically significant(t=5.417,5.974,all P<0.05).Conclusion The clinical curative effect of sodium hyaluronate combined therapy with celecoxib in the treatment of elderly knee osteoarthritis is reliable, can effectively improve pain in patients with clinical symptoms, reduce joint fluid of induced nitric oxide and the concentration of nitric oxide synthase, it is worthy of popularization and application in clinic.

Objective:To assess the effect of serum heat inactivation on the detection of 2019 novel coronavirus (2019-nCoV) specific IgM and IgG antibodies by colloidal gold method.Methods:The serum specimens were collected from a total of 106 Coronavirus Disease 2019 (COVID-19) patients and 52 control subjects. Both the fresh serum and the heat inactivated serum samples from the same patient were detected simultaneously with the 2019-nCoV IgM and IgG antibodies detection kit (colloidal gold method). According to the patient′s onset time, the positive rates of antibodies production profile were calculated. The influence of heat inactivation on the detection rates of antibodies at different stages of disease after onset was analyzed.Results:The test results of the specimens of the healthy control group before and after inactivation were all negative. For the 106 specimens of COVID-19 patients, the detection rates of 2019-nCoV specific IgM and IgG antibodies were reduced after heating at 56 ℃ for 30 min. The positive rates of IgM antibodies significantly decreased from 66.04% (70/106) to 43.40% (46/106) ( χ2=22.042, P=0.000), while the positive rates of IgG antibodies slightly decreased from 81.13% (86/106) to 76.42% (81/106) ( χ2=0.800, P=0.063). Further analysis revealed that there was a significant difference in the positive rates of IgM antibodies before and after heat inactivation in the 3rd, 5th and 6th week after onset. However, there was no statistically significant difference in the detection rates of IgG antibodies before and after serum heat inactivation in different periods of onset. Conclusions:Heat inactivation significantly decreased the detection rates of 2019-nCoV specific IgM antibodies, which may lead to serological false negative results.

Objective:To analyze the identification and antibiotics susceptibility of Herbaspirillum in catheter-related bloodstream infection, and improve the awareness and attention of the rare pathogenic microorganisms for clinicians and microbiologists. Methods:The bacterium was isolated from a positive blood culture of a hemodialysis patient with chronic renal failure. The smear of isolate was prepared, stained and observed by microscope. The single colonies were identified by mass spectrometry and VITEK 2 Compact identification and antibiotics sensitivity analysis system, respectively. Then, 16S ribosomal DNA (rDNA) was amplified and sequenced, and bacterial genome was sequenced.Results:The gram-negative bacilli was found in the positive blood culture bottle. After incubated on blood agar for 16 hours, milky white, bulging and non-haemolytic colonies were observed. The identification result was Burkholderia cepacian by VITEK 2_Compact and antimicrobial susceptibility testing showed resistance to aztreonam and polymyxin but sensitive to other drugs in N335 card. The isolate could not be identified by VITEK MS with clinical database. However, it was identified as Herbaspirillum huttiense/Herbaspirillum aquaticum with research database. The 16S rDNA of the strain was consistent with Herbaspirillum huttiense and Herbaspirillum aquaticum (more than 99%). High-throughput bacterial genome sequencing revealed that the isolate in this case shared 100% homology with Herbaspirillum huttiense subsp putei IAM 15032 in Genbank database, which confirmed that the isolate was Herbaspirillum huttiense. Conclusions:There are more and more environmental microorganisms evolved into human pathogenic bacteria. Herbaspirillum species are easy to be misidentified because its biochemical characteristics are similar to other strains.