Objective To compare the vibrating perception threshold (VPT) between normal subjects and subjects with cervical spondylosis of nerve root type, and to observe the clinical efficacy of acupoint injection at Quyuan (SI13) in releasing pain in cervical spondylosis of nerve root type.Method Sixty-three patients with cervical spondylosis of nerve root type were recruited and randomized into a treatment group of 35 cases and a control group of 28 cases. The treatment group received acupoint injection at Quyuan, and the control group received acupoint injection at Jiaji (EX-B2) points. The short-form McGill Pain Questionnaire (MPQ) was adopted. From the questionnaire, the Pain Rating Index (PRI), sensory (S), affective (A), and total (T) pain rating indexes, Visual Analogue Scale (VAS), and Present Pain Index (PPI) were used to comprehensively evaluate the pain improvement and to quantify the therapeutic efficacy, and the VPT was also considered. The Clinical Assessment Scale for Cervical Spondylosis (CASCS) by West China Rehabilitation Center was also used to analyze the result in the treatment group.Result After the intervention, the MPQ and VAS scores in the treatment group were significantly lower than those in the control group (P<0.05). The total effective rate of the treatment group was significantly higher than that of the control group (P<0.05). Meanwhile, the VPT was improved after the treatment.Conclusion Acupoint injection at Quyuan can produce a more significant efficacy than at Jiaji (EX-B2) in treating cervical spondylosis of nerve root type.

Objective To solve the problem that the rat retina paraffin sections are easily exfoliated from the slides and each layer are easily separation and fracture,we need to find a way to improve the retina paraffin section method and evaluate the tissue fixation. Methods We used 4 fixation liquids including 10%paraformaldehyde,4%paraformaldehyde, 4% paraformaldehyde and 95% alcohol and glacial acetic acid mixed liquid (FAA fixatiue solution ) combined with paraformaldehyde to fix the retinal tissue, and observed the fixation efficacy under microscope after HE staining. Results The effects of 10%paraformaldehyde and 4%paraformaldehyde fixed samples showed moderate separation and fracture of retina,but the HE staining retinal slices pre-treated by the FAA fixafive solution had bright and uniform color,although occasionally some parts of the retina were exfoliated from the slide, but it was not easy to take off,and had complete structure without separation and rupture. Conclusion The retina paraffin section fixed by FAA ixafive solution with 4% paraformaldehyde is superior to pure paraformaldehyde, and the paraformaldehyde concentration has no obviously influence on HE staining results.

Objective To explore the risk factors that may cause recurrent retinal detachment in rhegmatogenous retinal detachment(RRD)patients after surgery.Methods A total of 403 patients(403 eyes)with RRD diagnosed in the Department of Ophthalmology of the First Affiliated Hospital of Soochow University from October 2020 to April 2022 were included in this study.Among them,35 patients underwent the scleral buckling,79 patients underwent the pars plana vit-rectomy(PPV)+intravitreal gas tamponade,222 patients underwent the PPV+intravitreal silicone oil tamponade,and 67 patients underwent the PPV+vitreous silicone oil tamponade+silicone oil removal.The postoperative follow-up lasted for at least 3 months.Risk factors affecting one-time retinal reattachment after RRD surgery were analyzed by univariate and multivariate Logistic analyses,and Logistic regression was applied to construct a risk prediction model.Results Among the 403 eyes with RRD,369 eyes had retinal reattachment on the first try,and 34 eyes did not,with a one-time reat-tachment rate of 91.6％.The univariate analysis showed that the differences in axial length(AL),surgical approach,loca-tion of the tear,and size of the tear between patients with one-time retinal reattachment and those without reattachment were statistically significant(all P＜0.05).From the regression equation,it was found that the risk of non-reattachment af-ter RRD surgery of patients with an AL ≥ 26 mm was 4.248 times higher than those with an AL＜26 mm(P＜0.05).The multivariate Logistic regression analysis showed that AL,location of the tear,size of the tear,and surgical approach were risk factors for non-reattachment after RRD surgery(all P＜0.05).The Hosmer-Lemeshow test yielded P=0.165.The re-sults of the receiver operating characteristic curve analysis showed that the area under the curve to predict whether retinal re-detachment occurs after RRD surgery was 0.892(95％CI:0.832-0.953),and the sensitivity and specificity were 79.4％and 87.3％,respectively(P＜0.05).Conclusion AL is an independent risk factor for retinal re-detachment after RRD surgery.The prediction model constructed based on AL,location of the tear,size of the tear,and the surgical method can accurately predict whether retinal detachment will occur after RRD surgery.