Objective To find a new method to prepare the acellular matrix of the penile cavernous body. Methods Intact specimens of the penile cavernous body were collected from healty adult rabbits,treated with Triton-X100 and NH3?H2O,and then stained with HE.Light microscopy was employed to observe the acellular cells.Results Acellular matrix of the penile cavernous body was obtained after a 25-day treatment with Triton-X100 and NH3?H2O.The matrix was intact.HE staining showed that the matrix was composed of regularly arranged elastic fibers with wide intra-spaces without any cells inside.Conclusion Acellular matrix of the penile cavernous body can be prepared by using Triton-X100 and NH3?H2O.

Objective: To confirm the optimum ingredient of the filler in Weiling Capsules (Rhizoma Coptidis, Fructus Evodiae, Fructus Toosendan and Radix et Rhizoma Rhei. Methods: The dissolution of berberine in weiling capsules of four different prescriptions were determined. Results: The T 50 , T d and m of the capsules of whole extraction, extraction which contains 1/4 raw drugs were significantly different from that containing 1/2 raw drugs ( P 0.05), and there is no significant difference between them ( P

Objective To study the construction of tissue engineering fat filler and the survival of fat particles.Methods Stromal vascular fraction (SVF) was derived from inguinal subcutaneous fat of rabbits,mixed with autologus mature fat particles and with or without neuropeptide Y to construct PLGA composite tissue.The complex was subcutaneously transplantef into back sites of the rabbits.Based on the different combination five groups were divided:Group A:PLGA + mature fat particles without NPY;Group B:PLGA + mature fat particles + NPY;Group C:PLGA + complex of SVF with mature fat particles without NPY;Group D:PLGA + complex of SVF with mature fat particles + NPY;Group E:Complex of SVF with mature fat particles + small ball with NPY.Diffenrence of virous constructive ways and fat particle survival was evaluated by general observation,histological staining,fluorescence tracing at two weeks,one month and three month after operation.Results Group D was superior to groups A,B,C and E in survival volume,graft texture and vascularization at one mouth and three mouths.The fat average srvival rate was 57.5±2.5％.Fat cell grew well,and precursor cells proliferated and differentiated actively.Conclusions High quality tissue engineering materials are successfully established with SVF-mature fat particle complex,PLGA and NPY,which could obviously improve fat particle transplantation.

Genomic DNA of the two bacterial strains including Escherichia coli DH5? and Enterobacter cloacae E 26R were amplified by PCR with specific primers of ERIC sequence Each strain showed the stable and unique DNA fingerprint when PCR products were analyzed in agarose gel electrophoresis The stability of the genomic DNA fingerprint wasn't influenced by the templet from 10ng to 100ng In the fingerprints there were characteristic bands The unique band was influenced difficultly due to the change of the environment and condition of experiment DH5? and E 26R were mixed proportionately, and examined by ERIC PCR The result showed that the DNA fingerprints of the mixture were the superposition of each pure bacterium's Analysis of the main bands showed that DH5? can be examined by ERIC PCR when its concentration gets to 0 5% of the total mixed bacteria The study provides the reference for the research of soil microbe and environmental microbe, and especially can be used for quick identification and examination of the sundry bacterial contamination in the fermentation industry

Objective To explore techniques of endoscopic ultrasonography for nasal cavity and its accuracy. Methods Under the guidance of nasal endoscope, ultrasonography of nasal cavity was performed by using a 10 MHz,3. 3 mm section probes. Thirty nasal cavities of 15 normal canine were scanned under general anesthesia. The sink experiment was used to decrease the influence of the ultraphonic artifact and to correct the data error. Results In gray scale ultrasound,most mucous and submucous tissue within nasal cavity were hypoechoic, the septal cartilage was echoless, and the cartilaginous membrane of the septal cartilage showed a consecutive hyperechogenicity line. The average thickness of the nasal septum and the average thickness of the inboard mucous and submuous of the inferior turbinate were (1. 87 0. 33)mm and (2.96 0.36) mm respectively. The rich blood flowing signals were observed by CDFI in soft tissues mentioned above. Pulsed Doppler of the nasal septum showed a venous waveform with the velocity ranging from 3. 1 to 13.8 cm/s and the arterial waveform with the peak systolic velocity of 10 to 54.8 cm/s, resistance index ranged from 0. 31 to 0. 57. Pulsed Doppler of the inferior turbinate showed a venous waveform with the velocity ranging from 4. 0 to 17. 3 cm/s and the arterial waveform with the peak systolic velocity of 7.5 to 79.6 cm/s, resistance index ranged from 0.25 to 0.62. Conclusions With correction factor,nasal endoscopic ultrasonography was accurate in localizing structures with clear images and high resolution. It could be a new imaging modality of diagnosing nasal mucosal and submucosal disorderes.

Objective To investigate the association between serum 25(OH) D levels and the inci-dence of early-onset sepsis(EOS) in the very low birth weight infants(VLBWI) and the gestational age be-low 34 weeks. Methods The cord blood of 159 VLBWI were collected between January and December 2017,including 31 clinically diagnosed EOS and 128 non-EOS patients. Serum 25(OH)D<10 ng/ml was de-fined as severe vitamin D deficiency,25(OH)D 10 to 20 ng/ml as vitamin D deficiency,25(OH)D 20 to 30 ng/ml as vitamin D insufficiency and 25(OH)D >30 ng/ml as vitamin D sufficiency. Results There were no differences in gender,gestational age,birth weight and Apgar score between the EOS group and the non-EOS group(P>0. 05). Serum 25(OH) D was(9. 08 ± 4. 21) ng/ml in the EOS group and(11. 91 ± 5. 37) ng/ml in the non-EOS group(P＝0. 007). The rate of severe vitamin D deficiency was 67. 7%(21/31)in the EOS group and 41. 4%(53/128) in the non-EOS group. The rate of vitamin D deficiency was 32. 3%(10/31)in the EOS group and 52. 3%(67/128)in the non-EOS group. But there was no difference of vitamin D deficiency distribution in the two groups(P＝0. 152). The cut-off value of serum 25(OH)D level in predic-ting EOS was 10. 06 ng/ml. Conclusion The incidence of vitamin D deficiency is as high as 95%,calling for urgent attention on vitamin D supplementation in those VLBWI. Low 25(OH)D level( <10 ng/ml)might be predictive of EOS.

Objective:To investigate the clinical effect of posterior orbital septal fascia-levator muscle complex overlapping technique in the treating of mild ptosis.Methods:Clinical data of patients with mild blepharoptosis admitted to Peking University Shenzhen Hospital from January 2018 to January 2020 were retrospectively analyzed. All patients were treated by the same surgeon with folding of posterior orbital septal fascia-levator muscle complex. Postoperative complications were recorded. Binocular symmetry like excellent, good and poor at 6 months postoperatively was evaluated. The correction effect of blepharoptosis was evaluated 12 months after operation, including good correction, basic correction, poor correction and excessive correction.Results:A total of 85 patients(36 males and 49 females, 25.0±4.1 years old) were enrolled, including 62 bilateral and 23 unilateral blepharoptosis patients. The patients were followed up for 12 months. No complications such as hematoma, infection, exposed keratitis or fornical conjunctival prolapse occurred. Among the 85 cases, 63 cases had excellent binocular symmetry, 17 cases had good binocular symmetry, and 5 cases had poor binocular symmetry. The excellent/good rate was 94.1% (80/85). 72 cases of blepharoptosis were well corrected, 9 cases were basically corrected and 4 cases were poorly corrected. The effective rate was 95.3% (81/85).Conclusions:For mild ptosis, plication of the posterior orbital septal fascia-levator muscle complex is safe and effective operation method with minimal injury, and easy to grasp.

Objective To explore the effect of visualization health education of diet on diet compliance in with esophageal stenting. Methods A total of 56 esophageal neoplasms patients with esophageal stenting at Nanxishan Hospital of Guangxi Zhuang Autonomous Region were selected from January 2012 to June 2016. They were randomly divided into observation group and control group, 28 patients in each group. Patients in the control group received routine health education on diet knowledge, while patients in the observation group were provided with diet health education knowledge cards. Intervention time included the day been admitted to hospital, the day of surgery, one day after surgery and before leaving hospital. This study compared the diet compliance and the incidence of complications of patients between two groups. Results The diet compliance of patients in the observation group was better than that of the control group with a significant difference (P<0.01). The incidence of pectoralgia, fecal occult blood, stent displacement or abscission and gastroesophageal reflux of patients in the observation group was 57.1%, 53.6%, 3.6%, 14.3% lower than those (92.9%, 82.1%, 28.6%, 57.1%) of the control group with significant differences (P< 0.01). Conclusions Nurisng of visualization health education can effectively improve the diet compliance of patients with esophageal stenting and reduce the incidence of complications after esophageal stenting.

Objective:To investigate the clinical effect of posterior orbital septal fascia-levator muscle complex overlapping technique in the treating of mild ptosis.Methods:Clinical data of patients with mild blepharoptosis admitted to Peking University Shenzhen Hospital from January 2018 to January 2020 were retrospectively analyzed. All patients were treated by the same surgeon with folding of posterior orbital septal fascia-levator muscle complex. Postoperative complications were recorded. Binocular symmetry like excellent, good and poor at 6 months postoperatively was evaluated. The correction effect of blepharoptosis was evaluated 12 months after operation, including good correction, basic correction, poor correction and excessive correction.Results:A total of 85 patients(36 males and 49 females, 25.0±4.1 years old) were enrolled, including 62 bilateral and 23 unilateral blepharoptosis patients. The patients were followed up for 12 months. No complications such as hematoma, infection, exposed keratitis or fornical conjunctival prolapse occurred. Among the 85 cases, 63 cases had excellent binocular symmetry, 17 cases had good binocular symmetry, and 5 cases had poor binocular symmetry. The excellent/good rate was 94.1% (80/85). 72 cases of blepharoptosis were well corrected, 9 cases were basically corrected and 4 cases were poorly corrected. The effective rate was 95.3% (81/85).Conclusions:For mild ptosis, plication of the posterior orbital septal fascia-levator muscle complex is safe and effective operation method with minimal injury, and easy to grasp.

Objective:To investigate the impact of overweight/obesity on postoperative complications in breast reduction surgery.Methods:A retrospective study was conducted on patients who underwent breast reduction surgery in our hospital from 2016 to 2022. Basic patient information and postoperative complications, such as poor wound healing, infection, hematoma, nipple necrosis, etc., were collected. Patients were categorized into normal/low weight group and overweight/obese group according to their body mass index (BMI). The differences in postoperative complications between the two groups were compared and statistically analyzed.Results:The study included 82 patients, 48 in the normal/low weight group and 34 in the overweight/obese group. The gender, age, and surgical methods of the two groups of patients were relatively balanced, and there was no statistical difference. However, the incidence of postoperative complications was 50.0% (17/34) in overweight/obeseitg group, higher than 18.8% (9/48) in normal/low weight group. Common complications included delayed healing such as infection or dehiscence in 8 cases (9.8%), skin necrosis in 5 cases (6.1%), and hypertrophic scars in 5 cases (6.1%). The incidence of skin necrosis and delayed healing in the overweight/obese group was higher than that in the normal/low weight group, but the difference was not statistically significant ( P>0.05). Postoperative hematoma and hypertrophic scars occurred in both groups, while fat liquefaction and thrombosis only occurred in the overweight/obese group. Conclusions:Overweight/obesity is an important risk factor for postoperative complications in breast reduction surgery. Patients should be evaluated before surgery, and necessary measures should be taken to reduce the risk of complications.