Objective To investigate the effects of annonaceous acetogenin on proliferation and apoptosis in Raji cells and its mechanism.Methods Raji cells cultured in vitro were divided into control group,annonaceous acetogenin group and adriamycin group.Raji cells were effected by 6.25,12.5,25,50 ?g/mL annonaceous acetogenin.Proliferation of Raji cells were evaluated by MTT assays,apoptosis percentage was assessed by flow cytometry.Caspase-9 protein was detected by immunohistochemistry.Results The Raji cell proliferation rate of annonaceous acetogenin decreased compared with the control,that of 25,50 ?g/mL group were lower than adriamycin group,and it was related to the concentration,relying on the incubating time.The apoptosis rate was higher than control,that of 25,50 ?g/mL group were higher than adriamycin group,and it was related to the concentration and the incubating time.The expression of caspase-9 protein of annonaceous acetogenin group was higher than control,and it had a positively relationship with the concentration and incubating time.Conclusions Annonaceous acetogenin could inhibit cell proliferation in a dose-dependent and time-dependent manner in Raji cells,and it may induce Raji cells apoptosis by up-regulating caspase-9 expression.

OBJECTIVE： To analysis the adverse reactions of angiotension converting enzyme inhibitor（ ACEI） ， so as to deepen the understanding to the ARDs and improve the therapeutic effect of ACEI METHODS： The therapeutic effects and ARDs of ACEI were retrospectively reviewed and the incidence of ARDs induced by ACEI and the relationship between different ACEIs and their ARDs were analyzed RESULTS： （ 1） The incidence of ARDs of ACEI was 12％  Stimulating cough， parageusia， gastrointestinal tract discomfort， skin rash， itch of skin， hypotension， headache， dizziness， proteinuria and angioedema were the common symptoms （ 2） Of five kinds of ACEIs， captopril induced the highest incidence of ARDs and stimulating cough， parageusia， gastrointestinal tract discomfort， skin rash and itch of skin were the most common manifestations CONCLUSION： Stimulating cough， parageusia， gastrointestinal tract discomfort and skin rash are the most common symptoms of ARDs， and the symptoms will disappear when drug administration is discontinued

Objective To explore curative efficacy of Xianlinggubao capsules combined with Lugua polypeptide injection in treatment of vertebral osteoporosis.Methods 90 patients of vertebral osteoporosis from March 2014 to March 2015 in our haspital were selected as research objects and randomly divided into the observation group and control group,with 45 cases in each group.The control group were treated with Lugua polypeptide injection, while the observation group were treated with Xianlinggubao capsules combined with Lugua polypeptide injection.Then bone mineral density, osteocalcin, visual analogue scale (VAS), Oswestry disability index (ODI) score, curative effect in two groups after treatment were compared.Results Ater treatment, bone mineral density, osteocalcin in observation group was than that in control group [(0.99 ±0.13)g/cm2 vs(0.89 ±0.14)g/cm2, (41.05 ±5.30)μg/L vs(35.80 ±5.23)μg/L],the difference was statistically significant(P<0.05); VAS、ODI score in observation group were lower than those in control group[(1.21 ±0.30)分vs(4.65 ±1.08)分、(18.24 ±4.97)% vs(31.26 ±5.73)%],the difference was statistically significant (P<0.05).The total effective rate of observation group was statistically higher than that in the control group 95.55%(43/45),the difference was statistically significant vs77.77%( 35/45 ) ( P<0.05 ) .Conclusion Xianlinggubao capsules combined with Lugua polypeptide injection is well for vertebral osteoporosis,which can improve the bone metabolism of patients, promote bone growth and improve treatment efficacy.

To find a new method to identify Mori Cortex and its adulterants by analysis their ITS2 sequence of barcode,total genomic DNA was isolated from Mori Cortex and its adulterants. Nuclear DNA ITS2 sequences were amplified,and purified PCR products were sequenced. Sequence assembly and consensus sequence generation were performed using the CodonCode Aligner V3.0. The Kimura 2-Parameter (K2P) distances were calculated us-ing software MEGA 4.0. Identification analyses were performed using BLAST1, Nearest Distance and neighbor-joining (NJ) methods. The results showed the intra-specific genetic distance of Mori Cortex was 0, which were lower than inter-specific genetic distances between Mori Cortex and its closely related species (0.003-0.343). The ITS2 region is an efficient barcode for identification of Mori Cortex and its closely related species, which provides a scientific basis for fast and accurate identification and new method of Mori Cortex.

Tumor radioresistance is the leading cause of clinical radiotherapy failure and disease progression.Researches show that the occurrence of radioresistance is related to the cell cycle arrest,relevant gene change,tumor microenvironment change,autophagy,tumor stem cells and other factors.Studying the mechanism of radioresistance and looking for an effective method to avoid it is the key to improve the effect of radiotherapy,which can provide the probability of the prognosis of radiosensitivity.

Objective To investigate the mental health and the affecting factors on children cochlear implants and to provide the reference of comprehensive rehabilitation for them.Methods We used the Psychological Ques-tionnaire for 3~6 Years Children to evaluate the mental health status of 1 6 6 children of 3~4 years old with cochlear implants,and compare those with the standard values of children with normal hearing.We studied the affecting fac-tors on their psychological development.Results The pass rates in action,emotion and will,cognitive ability,sociali-ty,living habits and speech ability of children with cochlear implants decreased as their age increased.The scores of action,emotion and will,cognitive ability,sociality,living habits and speech ability of children with cochlear im-plants increased as the use time increased.The affecting factors on the psychological development of children with cochlear implants were the time length of usage and their residences before operation.Conclusion There were differ-ences between the children with cochlear implants and normal hearing in psychological development.We should fo-cus on their development of hearing and speech ability,and the psychological development.

Recent studies find a number of promising drug targets which can be applied for increasing tumor radiosensitivity in addition to normal tissue radioprotection,including oxygen free radical scavenger,drug targets in view of DNA damage repair and cell cycle,new targets inhibiting cell death,radioprotection mediated by growth factors,regulation of the cell signaling pathways,angiotensin-converting enzyme inhibitor.radiorestorative chemicals and so on.

To improve 3-ketosteroid-△1-dehydrogenase (KSDH) activity and the transformation level for androst-4-ene-3,17-dione,3-ketosteroid-△1 -dehydrogenase gene (ksdD) from Arthrobacter simplex was cloned into plasmid pWB980 and expressed in B. subtilis WB600 under the control of promoter P43. The molecular weight of expressed enzyme was about 55kDa by SDS-PAGE analysis. The activitities assayed by spectrophotometrical method of intracellular and extracellular soluble enzyme were 110 ± 0.5mU and 15 ± 0.6mU per milligram of protein respectively. The transformation rate of androst-4-ene-3,17-dione by the B. subtilis recombinant cells was 45.3%. Compared with Arthrobacter simplex, the enzyme activity of KSDH expressed in B. subtilis was improved about 30 fold, and the transformation level of androst-4-ene-3,17-dione by the B.subtilis recombinant cells was improved about 10 fold. The recombinant B. subtilis cells used in biotransformation of steroids provided a new way for steroid medicines production.

Artemisinin and its derivative is a kind of efficient,quick and low toxicity of anti-malarial drug.In recent years,we find that artemisinin drugs can not only against malaria,but also have pharmacological effects of immunosuppression,antiviral and anticancer.A number of studies have confirmed that artemisinin and its derivatives have the radiosensitization effects in nasopharyngeal carcinoma,cervical cancer,lung cancer and glioma,and their mechanisms are related to decreasing Weel protein expression,increasing Cyclin B1 protein expression,blocking G2-M phase and cell apoptosis.

Objective To express and purify the envelope ( E) protein of Japanese encephalitis virus (JEV) in soluble form.Methods Various prokaryotic expression vectors , host strains and induction conditions including time and temperatures were screened to obtain an optimum prokaryotic expression system for JEV E protein in soluble form .The expressed protein was purified by using nickel column chromatography and gel filtration chromatography .Results The soluble JEV E protein , accounting for 23% of the totally bacteria soluble protein was effectively expressed by using the recombinant plasmid PBCX -E406 at low tem-perature.The purity of the expressed protein reached up to 85%after the purification by using nickel column and gel filtration chromatography .Conclusion Soluble JEV E protein was successfully expressed and puri-fied in a simple and efficient way .It would provide a useful tool for further investigation on JEV infection , attenuation mechanism of JE live vaccine strain SA 14-14-2 and the quality control of JE vaccine .It can also be used for the development of diagnosis assay for JEV .