Objective This paper investigated the effects of intermittent hypoxia exposure (IHE) on HRPRP?SOD?GSH-PX?MDA and exercise performance. Methods Fifteen male students were randomly assigned to group of control and IHE. The IHE group intermittently inhaled different hypoxic gas mixtures (O 2% were 14%, 12%, 10%, respectively),50～60 mins daily for 4 weeks. Both groups completed Bruce protocol exercise on the treadmill before and after IHE. The HRPRP, SOD, GSH-PX, MDA were measured at rest and post exercise respectively. Results The concentration of HRPRP and the activity of SOD, GSH-PX were increased as well as VO 2max and the time to exhaustion. Conclusion IHT is effective on improving the antioxidant capacity and sports performance.

Objective To explore the feasibility of using Zeocin resistance as a new positive selection marker for the genetic manipulation of Candida albicans (C.albicans).Methods The susceptibility of C.albicans strain CAI4 to Zeocin was determined using a broth microdilution method in pH range from 6.0 to 8.0.The ZeoR expression cassette was amplified from plasmid pGAPZ-α-A by polymerase chain reaction (PCR).An integrated vector used to knockout AAF1 gene was constructed by gene splicing.Then the C.albicans CAI4 was transformed with this integrated plasmid containing the ZeoR expression cassette using lithium chloride method and the target AAF1 gene was replaced.The recombinant C.albicans was screened and the genome was extracted and amplified by PCR with two pairs of primers,then confirmed using genetic method.Results The C.albicans CAI4 was sensitive to Zeocin in pH range from 6.0 to 8.0.And the concentration of Zeoein(100 mg/L) at pH 7.0 was used to select recombined C.albicans.The recombined integrated vector was confirmed by DNA sequencing.The ZeoR expression cassette was detected using PCR method in recombined C.albicans.The AAF1 gene was replaced by ZeoR gene.Conclusion A new vector for targeted gene disruption in C.albicans is successfully constructed.

In order to reduce the impact of various noise in pulse signal, the quality estimation and filtering algorithms based on cyclostationarity are proposed to reprocess pulse signal. First, A quality evaluation index of pulse signal which named quality factor is defined by cyclic spectrum to describe the quality variation of the pulse signal affected by noise; Second, a cyclic correlation matched filter (CCMF) is designed to remove noise. The simulation of pulse signal is produced by ourselves and noise signal is provided by MIT-BIH physiological database are used to test the function of proposed method, and then the method is applied to the actual pulse signal. The results show that the quality factor can accurately reflect the quality of the pulse signal and the CCMF can effectively remove noise from pulse signal.
Algorithms ; Databases, Factual ; Heart Rate ; Humans

Objective To explore the effects of mental practice on upper extremity function after stroke. Methods Thirty sub-acute stroke patients were randomly divided into a treatment group ( n=15 ) and a control group (n=15). The patients in the control group were treated with conventional therapy. The patients in the treat-ment group were treated with motor imagery therapy in addition. All patients were assessed using the Fugl-Meyer mo-tor assessment (FMA) and the motor assessment scale (bIAS) before treatment and after 2, 4 and 8 weeks of treat-ment. Results After 2 weeks of treatment, average MAS scores in the treatment group improved significantly com-pared with before treatment, but there was no significant difference between the two groups. After 4 weeks, FMA and MAS scores in the two groups had improved, and the FMA scores in the treatment group were significantly higher than those of the control group. After 8 weeks, the FMA and MAS scores of both groups had further improved significant-ly, but the average FMA and MAS scores in the treatment group were now significantly higher than those in the control group. Conclusions Mental practice can improve the functional performance of the upper extremities of stroke pa-tients.

The superoxide anion radical (Of) produced in the alkaline dimethyl sulfoxide (DMSO) system was directly measured by electron paramagnetic resonance (EPR) at low temperature. This reaction was used as a model system for generating O2-. Astragalus membranaceus, Codonopsis pilousla, Paeonia laetiflora, Angelica sincnsis and Sophora flavescens, traditional natural medicines which have the effect of delaying anility, were added into the model system to observe their intensifying or inhibiting effect on EPR signals of O2. These results suggest that these traditional natural medicines have the mostly eliminating effect on Of produced in the alkaline DMSO system.

The effects of qi-tonics and blood-activating decoction on acute myocardial infarction and ischemia-reperfusion induced arrhythmias were studied in anaesthetized open chest SD rats. Three days before coronary artery occlusion, the qi-tonics and blood-activating decoction was perfused into the stomach of the rats in the experimental group (10 ml/kg weight) once a day, and 4d later, the coronary artery was occluded in anaesthetized open chest rats. The results demonstrated that qi-tonics and blood-activating decoction could reduce significantly the incidence and severity of arrhythmias caused by myocardial infarction and ischemia-reperfusion. The qi-tonics and blood-activating decoction could decrease the amount of the superoxide anion radicals (O2-) produced in the alkaline dimethyl sulfoxide (DMSO) system measured by electron paramagnetic resonance (EPR) at low temperature. The effect of qi-tonics and blood-activating decoction on O2-may be one of the mechanisms for its antiarrhythmic effect during reperfusion.

Self-made molecule imprinting polymer-solid phase extraction column was used in extracting nerve agent degradation products in rice. The extraction was then examined by capillary electrophoresis. The method was simple, reliable and sensitive. The calibration curve showed a good linearity for the nerve agent degradation products in rice was in the concentration range of 0.2～5.0　μg/g and the detection limits were 0.05μg/g. The RSD of the method was less than 6.2%.

Objective To explore the application of atomic force microscopy( AFM ) on the research of morphology of the rabies viruses. Methods To prepare the rabies virus CTN-1v strains by ultracentrifugation, and observe it with transmission electron microscopy (TEM) which negatively stained by phosphotungstic acid. Then study the morphology of rabies virus with AFM based on the result of TEM. AFM image applies the tapping mode to rabies virus without any further treatment in air at room temperature. Results The TEM image is two-dimensional image which can be seen the classical bullet-shaped structure,and the spike structure can also be seen. The AFM image showed the rabies virus morphology with three-dimensional image which can shows the characteristics of the virus surface and edge. The rabies virus particle was successfully observed by TEM or AFM methods. Conclusion It's the first time to get the three-dimensional morphological structure of rabies virus by atomic force microscopy, compared with transmission electron microscopy, AFM is a new research tool for viral morphology study with the advantages of simple sample preparing and intuitionistic and visible interface for researchers.

[Summary] The aim of this study was to investigate the changes of sex hormone in male patients with type 2 diabetes and complicated with non-alcoholi fattty liver disease (NAFLD),and to detect the characteristics of the metablic indexs.A total of 112 patients were enrolled and divided into NAFLD group (n =54) and non-NAFLD group (n =58).All patients were measured for waist circumference,body mass index,metablic index,sex hormone,and serum transaminase.Compared with non-NAFLD group,the levels of fasting blood glucose,2 hour postprandial blood glucose,HbA1C in NAFLD group were significantly elevated [(8.75 ± 2.58 vs 7.79 ± 1.89) mmol/L,(18.12 ± 3.95 vs 15.63 ± 3.89) mmol/L,(11.96 ±4.85 vs 10.05 ±4.15)％,all P＜0.05].In addition,total cholesterol,triglyceride,body mass index,fasting insulin,and postprandial insulin in NAFLD group were significantly elevated [(4.97± 1.02 vs4.15±0.92) mmol/L,(2.74±2.25 vs 2.01± 1.45)mmol/L,(27.34±3.93 vs 22.38±3.39) kg/m2,(9.62 ± 5.80 vs 6.18 ± 4.21) μIU/ml,(72.71 ± 109.70 vs 31.72 ± 42.27) μIU/ml,all P＜0.05];while the total testosterone and free testosterone were lowered dramatically[(14.18 ±6.39 vs 18.21 ±6.14) nmol/L,(0.32 ± ± 0.03 vs 0.39 ± 0.08) ng/dl,both P＜0.05].Male patients with type 2 diabetes mellitus complicated with NAFLD are more prone to have disturbance in the lipid metabolism and had larger percentages of obesity and with lower level of testesterone.

Objective Toinvestigate the related risk factors of newly diagnosed diabetes among residents aged over 40 years in Nanjing City , and to provide evidence for diabetes prevention. Methods The clinical data were collected from the diabetes epidemiologic investigation among residents aged 40 -79 years from 6 communities in Nanjing City. Univariate and multiple logistic regression analyses were performed to analyze the potential risk factors for newly diagnosed diabetes. Results The prevalence rate of new diagnosed diabetes from 8039 subjects was 10.87%. A higher prevalence rate of new diagnosed diabetes was found in male than that in female (13.15% vs. 9.74%, P < 0.01). Multiple logistic regression analyses revealed that the main risk factors for newly diagnosed diabetes were male, age, family history, body mass index (BMI) and less physical activity after adjusting other factors (adjusted OR 1.339-1.862, P < 0.05). Conclusions Male, age, family history, body mass index (BMI) and less physical activity may be the main risk factors for diabetes among residents in Nanjing City.