Objective:To research the age-related changes of thymocyte subsets and Notch 1,Presenilin 1,Presenilin 2,HES1 mRNA expression in senescence-accelerated mice during aging,and analyze the relation of the two changes.Methods:Thymocyte subsets were assayed by flow cytometry,the expression of Notch 1,Presenilin 1,Presenilin 2,HES1 mRNA was detected by quantitative real-time PCR fluorescence assay.Results:The rate of CD4+CD8+ double-positive(DP) cell of thymocytes was fairly high in first-and second-week-old SAM,and began to decrease at four-week-old,but no significant difference was observed between SAMP8 and SAMR1 of the same age.The rate of CD4+CD8-single-positive(SP) cell of thymocytes in SAM was age-related increased,yet no significant difference was overserved between SAMP8 and SAMR1,except that it was much lower at the eighth week in SAMP8 than in SAMR1.The rate of CD4-CD8+ SP cell of thymocytes in SAM was also age-related increased,but it was obviously higher in SAMP8 than in SAMR1 after the fourth week.The expression of Notch 1,Presenilin 2 and HES1 gene is age-related increased.The Notch 1 gene was higher at each week in SAMP8 than in SAMR1,but presenilin 2 and HES1 were observed higher in SAMP8 than in SAMR1 only after the fourth week.On the contrary,Presenilin 1 gene was age-related decreased,and it was lower in SAMP8 than in SAMR1 after the fourth week.Conclusion:It suggestes that the expression level of Notch 1,Presenilin 2 and HES1 gene positively correlated with the differentiation of CD4-CD8+ SP thymocyte,while presenilin 1 gene evidenced a significant negative correlated with it.

Objective:To research the effect of Liuwei Dihuang decoction and its decomposed recipes on gene expression related to thymocyte differentiation in normal mice and SAMP8.Methods:The expression of Notch1,Presenilin1,Presenilin2,HES1 mRNA was detected by quantitative real-time fluorescence PCR assay.Results:After oral administation of Liuwei Dihuang decoction(5,10,20 g/kg),the expressions of Presenilin2 and HES1 gene in thymocytes of normal mice were increased.After oral administation of Liuwei Dihuang decoction(10 g/kg)and SB,SX,the expressions of Presenilin2 and HES1 in thymocytes of normal mice were decreased.Conclusion:It is suggested that Liuwei Dihuang Decoction can increase the Notch signal intensity in the normal mice thymocytes and decrease that in SAMP8,which is immuno-senescenced mice.

Objective: To provide an important tool for the study of diagnose and treatment of prostate cancer (PCa) osseous metastasis and change of bone stress force on prostate cancer (PCa) osseous metastasis and a platform, which is more congruous to clinical process, for prevention and cure of neoplastic bone metastases, and to carry out the construction and improvement of animal models of PCa with different positional osseous metastasis in vivo.Methods: Different gradient concentrations of RM-1 cells were inoculated into the cavity of left femoral bone or lumbar vertebra of mice (C57BL/6) respectively.The change of mouse activity, tumor formation, tumor size and survival time were observed respectively.And the femur tissue and spinal tissue were obtained from the mice after death.The gray value of iconography were measured by imageological examination of femur tissue, and the final histopathological examination were taken to determine the tumor type in both femur and spinal tissue.Results: The tumor growth could be touched at the puncture site in all the mice after inoculated for 7 days.There were no obvious differences in the time of tumorigenesis, the rate of tumor growth and tumor size among the mice in the same group (P>0.05).As the result, the construction femoral bone and lumbar vertebra metastatic models of PCa had been confirmed by iconography and pathology detection.At the same time, the survival time of the mice inoculated with low concentrations of PCa cells was obviously longer than that of high concentrations of PCa cells (at least 2 weeks longer).Conclusion: The animal models with different positional osseous metastasis (limbs and axial skeleton) of PCa using the same PCa cells (RM-1) had been first constructed successfully in our study.At the same time, a high success rate of construction of PCa animal model with bone metastasis was obtained by femoral bone marrow cavity injection of PCa cells.The rate of tumor growth was rapid, animal survival time was appropriate, and the PCa animal model with bone metastasis can be stably reproduced by our method.These animal models can be used to explore the pathogenesis of different positional PCa bone metastasis and provide a new platform, which were more congruous to clinical process, for prevention and cure of neoplastic bone metastases.

This study was aimed to acquire the dynamic changes of chlorogenic acid and luteoloside of honeysuckle at different collecting periods in order to decide the best harvesting time of honeysuckle in Donghai County, Jiangsu Province. The content determination method used in the detection of chlorogenic acid and luteoloside of honeysuckle was from the 2010 edition of the Chinese Pharmacopoeia. The skills of HPLC fingerprint characteristic features, and the yield of pressed flowers were combined in the evaluation of honeysuckle at the three-green period, two-white pe-riod, great-white period, silver-flower period and the golden-flower period. The results showed that the content of honeysuckle at different blossoming stages had obvious changes in content of chlorogenic acid and luteoloside, as well as the pressed flower quality and yield of the flower. It was concluded that the optimal harvest time of honey-suckle was for the two-white period and the great-white period, which was consistent with the real origin.

Objective To discuss the clinical correlation between chronic prostatitis (CP) and erectile dysfunction (ED).Methods A retrospective analysis was made on the clinical data from patients who were diagnosed as CP in urology department of the First People's Hospital of Yunnan Province and the First Affiliated Hospital of Sun Yat-sen University.Patients' data were collected from July 2005 to July 2012.All the patients were diagnosed as CP based on their clinical manifestation,the NIH Chronic Prostatitis Symptom Index,prostatic fluid examination,routine urine examination and ultrasonography.The condition of erectile function was inquired on every patient and recorded.Results There were 4 315 patients diagnosed as CP in present study,while their age ranged from 17 to 64 years (average,31 years).Sixty-one (1.41％) patients were identified as moderate and severe types of ED (International Index of Erectile Function 5 ≤ 11),while 42 (0.97％) patients were confirmed as ED after they got CP.435/4 315 patients (10.08％)felt obviously sexuality descent after they got CP,and the sexuality was reported improved in 426/435 pa-tients after prostatitis symptomatic relief.Conclusions The incidence rate of ED in CP patients is not ob-viously higher than that in common community.And sexuality descent in patients might be caused by localindisposition and anxiety after CP.