OBJECTIVE: To optimize the preparation technology of phloroglucinol phospholipids complex and study its physicochemical properties.METHODS: The preparation technology for phloroglucinol phospholipids complex was optimized taking the molar ratio of phloroglucinol to phospholipids(A),reaction temperature(B) and reaction time(C) as factors and complex ratio of phloroglucinol and phospholipid as index.The dissolubility and oil/water partition coefficient of the complex in different solvents were determined and compared with crude drug,and the new complex's physicochemical properties were validated by UV and IR(infrared spectra).RESULTS: The optimized preparation conditions for phloroglucinol phospholipids complex were obtained as follows: A was 1∶2;B was 60 ℃ and C was 2 h.The complex ratio remained at above 98%.Compared with phloroglucinol crude drug,the phloroglucinol phospholipids complex in water or chloroform showed increase in both dissolubility and oil/water partition coefficient.UV and IR confirmed that no new complex had been formed from phloroglucinol and phospholipids.CONCLUSION: The optimized technology greatly enhanced the dissolubility and lipotropy of the phloroglucinol phospholipids complex.

ObjectiveTo investigate the effect of neoadjuvant chemotherapy in locally advanced cervical cancer on cell cycle,proliferation,and evaluate the feasibility of proportion of cell in different phase 　and proliferating cell nuclear antigen(PCNA) as sensitive indices to assess chemotherapeutical sensitivity and therapeutical effect.MethodsForty-nine cases of locally advanced cervical cancer were divided into response group and no-response group according to the clinical efficacy of neoadjuvant chemotherapy.Compared the proportion of cell in different phase and proliferation index of PCNA between two groups.The clinical therapeutic effect was evaluated after 4 weeks in the second neoadjuvant chemotherapy regimen.Results In 49 patients with locally advanced cervical cancer,clinical effective of 39 cases (response group),no effective of 10 cases(no-response group).The S-phase proportion of cell in the pre-neoadjuvant chemotherapy and post-neoadjuvant chemotherapy of response group[ (21.47 ± 5.21 )％ and(18.32 ±5.07)％] were higher than those of no-response group [ (9.63 ± 2.58)％ and ( 10.14 ± 2.32)％ ] (P ＜ 0.05 ).The proliferation index of PCNA of cervical cancer in the pre-neoadjuvant chemotherapy of response group [ ( 81.67 ± 7.14)％ ] was higher than that of no-response group [ (66.99 ± 2.29 )％ ] (P ＜ 0.05 ).Conclusion The S-phase proportion of cell and proliferation index of PCNA in the pre-neoadjuvant chemotherapy are important indexes to assess chemotherapeutical sensitivity and therapeutical effect.

Objective To investigate the association of primary liver cancer(PLC)with the mutations of HBV precore and basic core promoter(BCP)genes.Methods The serum markers of hepatitis B and the quantities of serum HBV DNA were detected in 144 HBsAg-positive PLC patients.The precore and BCP gene mutations in patients with HBeAg-negtive and HBV DNA-positive were detected by real-time PCR.One hundred and twenty chronic hepatitis B(CHB)patients were randomly selected to serve as the conol.Results There were 46(3 1.94%)patients with HBeAg-positive and 98(68.06%)patients with HBeAg-negative.In 98 HBeAg-negative patients,56(57.14%)were HBV DNA-positive,in which 43 (76.79%)were with precore 1896 gene mutations,50(89.29%)were with BCP1762/1764 gene mutations.and 38(67.86%)were with both gene mutations.Precore 1896 and BCP1762/1764 gene mutation rates in PLC patients were much higher than those in CHB patients(χ2=9.36 and 5.77,P＜0.05).Conclusion PLC may be associated with the mutations of HBV precore anti BCP genes.

Objective To investigate the needs of nursing-based continuing home care in old patients with chronic diseases in communi-ty. Methods From June to August, 2016, 14 old patients with chronic diseases were purposively sampled, and interviewed with semi-struc-ture. The data were collected and refined with phenomenological analysis. Results The patients were very positive in nursing-based continu-ing home care. The main requirements included the knowledge about chronic diseases, psychological comforts, rehabilitation nursing, daily security help and medical insurance support. Conclusion It is necessary to support the continuing home care for old patients with chronic diseases, and strengthen the profesional nursing team building in community.

significantly save the time of diagnosis and facilitate the clinical application of large samples.

Objective To investigate the effect of combined antiretroviral therapy (cART) on anal human papillomavirus (HPV) infection in human immunodeficiency virus (HIV)-infected men who sex with men (MSM).Methods HIV-infected MSM naive of cART who visited Wuhan Dermatological Hospital from June 2012 to December 2013 were enrolled in a longitudinal study before starting cART,including 81 HIV-positive and 50 HIV-negative cases.HPV infection situations between HIV-positive and HIV-negative MSM were compared.And anal HPV infection rate and condyloma acuminate incidence of HIV-positive MSM before and after cART were also compared.HPV genotyping was performed by universal primer PCR and reverse dot hybridization.The statistical analysis was done by t test or x2 test.Results The prevalence rates of HPV infection,high-risk HPV infection and multiple HPV subtypes infection in HIV-positive MSM were all significantly higher than those in HIV-negative MSM (91.4 ％ vs 62.0 ％,75.3 ％ vs 30.0 ％,56.8 ％ vs 20.0 ％,respectively,x2 =16.75,26.05,and 19.10,respectively,all P＜0.05).The prevalence rates of anal HPV infection,high-risk HPV infection and multiple HPV subtypes infection in 77 HIV-positive MSM at month 36 of cART were all significantly decreased than baseline (90.9％ vs 74.0％,75.3％ vs 44.2％ and 57.1％ vs 41.5％,respectively,x2 =7.590,15.551,and 3.741,respectively,all P＜0.05).HPV16 and HPV43 infection rates were reduced from 27.3％ at baseline to 15.6％ and 13.0％,respectively at month 36 of cART (x2 =16.92 and 14.86,respectively,both P＜0.05).Condyloma acuminate incidence also reduced from 16.9％ at baseline to 5.2％ at month 36 (x2 =4.069,P＜0.05).Conclusions The prevalence of anal HPV infection in HIV-positive MSM is higher than HIV-negative MSM.cART could reduce the prevalence of anal HPV infection rate and condyloma acuminate incidence,especially high-risk HPV infection.

OBJECTIVE：To study the influential factors for green innovation in pharmaceutical enterprises based on grounded theory so as to provide reference for pharmaceutical enterprises to enhance their green innovation ability and the government to issue relevant green policies. METHODS ：The grounded theory research methods were used to select enterprise samples and follow-up research. Firstly ，56 staffs from 22 sample enterprises （all were pharmaceutical companies ）were interviewed in depth one-on-one and face-to-face on issues related to the influential factors for green innovation. Then ，open coding ，spindle coding and selective coding were carried out on the conversation record data ，and conduct theoretical saturation testing was conducted. Finally ， the influential factor model of green innovation in pharmaceutical enterprises was constructed ，and its main influential factors were analyzed. RESULTS & CONCLUSIONS ：The government demand ，social demand ，industry demand ，green innovation ability ， environmental awareness of leaders and overall vision of leaders have significant influence on the green innovation of pharmaceutical enterprises. Among them ，leadership decision （including leaders ’environmental awareness and leadership pattern ） is the internal decisive factor ，green innovation ability （including green innovation investment capacity ，green innovation research and development capacity ，green innovation transformation capacity and green innovation production capacity ）is the internal driving factor ，social system （including government needs ，social needs and industry needs ）is the external factor ；they are the main factors affecting the green innovation of pharmaceutical enterprises ，and they all have a positive effect on the green innovation of pharmaceutical companies.

OBJECTIVE To study the protective effects of twin drugs of tetramethylpyrazine-scutellarein （TMSC4） on cerebral ischemia-reperfusion injury （CIRI） model rats and its mechanism. METHODS One hundred and five SD rats were randomly divided into sham operation group， model group， scutellarein group （0.7 mmol/kg）， tetramethylpyrazine group （0.7 mmol/kg）， and TMSC4 low-dose， medium-dose and high-dose groups （0.35， 0.7， 1.4 mmol/kg）， with 15 rats in each group. Sham operation group and model group were given constant volume of normal saline intragastrically， and other groups were given relevant drug intragastrically， once a day， for consecutive 14 d. Except for sham operation group， all other groups were treated to establish the CIRI model using the thread occlusion method. After 2 hours of ischemia and 22 hours of reperfusion， the brain index and brain water content of the rats were measured. Serum levels of interleukin 1β （IL-1β）， IL-6 and tumor necrosis factor α （TNF-α）， the levels of superoxide dismutase （SOD）， malondialdehyde （MDA）， glutathione peroxidase （GSH-Px） and catalase （CAT） in brain tissues， the situation of neuronal cell apoptosis， and the protein expressions of B-cell lymphoma 2 （Bcl-2）， Bcl-2-associated X protein （Bax） and cleaved-caspase-3 were evaluated. RESULTS Compared with sham operation group， the brain index， brain water content， the serum levels of IL-1β， IL-6 and TNF-α， the levels of MDA in brain tissues， the brain cell apoptosis and the protein expressions of Bax and cleaved-caspase-3 in model group were significantly increased （P＜0.05）； the levels of SOD， GSH- Px and CAT and the protein expression of Bcl-2 in brain tissues were significantly decreased （P＜0.05）. Compared with model group， the above indexes of rats were reversed significantly in administration groups （P＜0.05）， while the reverse effects of TMSC4 medium-dose and high-dose groups were significantly better than those of scutellarein group and tetramethylpyrazine group （P＜0.05）. CONCLUSIONS TMSC4 has a certain protective effect in CIRI model rats， the mechanism of which may be related to relieving inflammatory reaction and oxidative stress， inhibiting cell apoptosis.