1.A comparative study on the effect of BCG-PSN and thymopeptides on T-lymphocyte subsets of normal and immunosuppressed mice.
Yunhua, DENG ; Yingling, CHEN ; Xingping, CHEN ; Yongxi, LI ; Liyi, ZHOU
Journal of Huazhong University of Science and Technology (Medical Sciences) 2003;23(4):339-43, 347
To compare the effects of polysaccharide nucleic acid fraction of bacillus calmette guerin (BCG-PSN) and thymopeptides on T-lymphocytes of normal and immunosuppressed mice, CD4+ and CD8+ T-lymphocyte subsets of single nucleic cell in thymus, spleen and peripheral blood were detected successively by flow cytometry after application of BCG-PSN and thymopeptides. Meanwhile, CD4+/CD8+ ratio was also calculated. The results showed that both BCG-PSN and thymopeptides could decrease the proportion of CD4+ CD8+ T-lymphocyte subsets in the thymus, at the same time increase CD4+ T-lymphocyte, CD8+ T-lymphocyte proportion in the three tissues. The fluctuation in amplitude was greater in thymopeptides group than that in BCG-PSN group. It is concluded that acting location of thymopeptides is in thymus, its stimulating action is stronger than that of BCG-PSN, while BCG-PSN not only accelerates the differentiation in thymus, but also has some direct stimulation to peripheral CD4+ T-lymphocytes, and can maintain CD4+/CD8+ ratio within normal range. So, BCG-PSN is safer.
Adjuvants, Immunologic/*pharmacology
;
Immunocompromised Host
;
Mycobacterium bovis/*chemistry
;
Nucleic Acids/pharmacology
;
Peptide Fragments/*pharmacology
;
Polysaccharides, Bacterial/*pharmacology
;
T-Lymphocyte Subsets/*drug effects
;
Thymus Gland/chemistry
2.Predictive value on chemotherapeutic sensitivity of the changes of cell cycle and proliferation on locally advanced cervical cancer in the pre-neoadjuvant chemotherapy and post-neoadjuvant chemotherapy
Hongwen YANG ; Zhongdong CHEN ; Bo ZHOU ; Yongxi QIN ; Qiang ZHAO
Chinese Journal of Postgraduates of Medicine 2012;35(3):3-6
ObjectiveTo investigate the effect of neoadjuvant chemotherapy in locally advanced cervical cancer on cell cycle,proliferation,and evaluate the feasibility of proportion of cell in different phase and proliferating cell nuclear antigen(PCNA) as sensitive indices to assess chemotherapeutical sensitivity and therapeutical effect.MethodsForty-nine cases of locally advanced cervical cancer were divided into response group and no-response group according to the clinical efficacy of neoadjuvant chemotherapy.Compared the proportion of cell in different phase and proliferation index of PCNA between two groups.The clinical therapeutic effect was evaluated after 4 weeks in the second neoadjuvant chemotherapy regimen.Results In 49 patients with locally advanced cervical cancer,clinical effective of 39 cases (response group),no effective of 10 cases(no-response group).The S-phase proportion of cell in the pre-neoadjuvant chemotherapy and post-neoadjuvant chemotherapy of response group[ (21.47 ± 5.21 )% and(18.32 ±5.07)%] were higher than those of no-response group [ (9.63 ± 2.58)% and ( 10.14 ± 2.32)% ] (P < 0.05 ).The proliferation index of PCNA of cervical cancer in the pre-neoadjuvant chemotherapy of response group [ ( 81.67 ± 7.14)% ] was higher than that of no-response group [ (66.99 ± 2.29 )% ] (P < 0.05 ).Conclusion The S-phase proportion of cell and proliferation index of PCNA in the pre-neoadjuvant chemotherapy are important indexes to assess chemotherapeutical sensitivity and therapeutical effect.
3.Prevalence and risk factors of human papillomavirus infectious in HIV-positive women
Yongxi ZHANG ; Yan XIONG ; Xien GUI ; Shuhui CHEN ; Yuping RONG ; Hongbing CAI ; Hongbing LIAO
Chinese Journal of Clinical Infectious Diseases 2011;04(2):79-82
Objective To evaluate the prevalence and risk factors of human papillomaviruses (HPV)infection among human immunodeficiency virus(HIV)-positive women.Methods Totally 178HIV-positive and 122 HIV-negative women were enrolled.Structured interviews,peripheral CD4 + T cells counts and cervical specimens were obtained.Polymerase chain reaction(PCR)assay was used to identify HPV types. SPSS 16.0 was used for statistical analysis,and logistic regression was used to identify independent prognostic factors for high-risk HPV infection. Results HPV positive rate,high-risk and multiple HPV infection rates were 38.2% vs.12.3% ,35.4% vs.8.2% ,and 13.5% vs.3.3% in HIVpositive women and HIV-negative women,respectively,and the differences were of statistical significance (x2 =24.77,29.08 and 8.91,P <0.05).The common types of high-risk HPV were similar between HIV-positive and HIV-negative women(HPV16,52,58 and 18).CD4 + T count < 350/pL,HIV-positive in husband,and HIV infection through sexual contact were risk factors for high-risk HPV infection in HIV-positive women.Conclusion sThe prevalence of HPV infection in HIV-infected women is high,especially for high-risk HPV infection and multiple infection.High-risk HPV infection usually occurs in HIV-positive women with low immune status,HIV infection through sexual contact and HIV-positive husband.
4.Application of ultrasound-guided Mammotome in the diagnosis and treatment of breast neoplasms
Jian SUN ; Ang DING ; Taiming SUN ; Maoli WANG ; Yuchun JIN ; Hongliang CHEN ; Yongxi YUAN
Clinical Medicine of China 2010;26(11):1219-1221
Objective To summarize and explore the clinical value of Mammotome technology in the diagnosis and treatment of breast neoplasms. Methods Nine hundred and eighty-seven breast neoplasms of 710patients were biopsied and excised with the aid of ultrasound-guided Mammotome system. The malignant neoplasms,according to the histological report,were radically removed during the operation without delay. All patients enrolled into the study were followed up closely on the complcations and satisfaction degree. Results All the operations except one were successful and got the sufficient specimen to perform the pathological examination,the biopsy achievement rate was 99. 8% . Both the sensitivity and specificity of the diagnosis were 100% in 16 breast tumors. Complete resection rate was 99%. The incision length was about 3 mm,showing good cosmetic outcome. Complcations were mild,mainly composed of haematoma and ecchymosis. The patients' general satisfactory rate was 93%. Conclusions This technique can help achieve biopsy of breast neplasm and excision of benign lesions at the same time with cosmetic result Further attention should be paid to reduce the lesion residue and hemorrhagic complications.
5.Industry distribution of 1392 occupational hazard enterprises and their current status of occupational health management.
Xinxia LIU ; Guoxian HUANG ; Yimin LIU ; Zhiping GUO ; Yongxi LIANG ; Hao CHEN ; Jianqing FENG
Chinese Journal of Industrial Hygiene and Occupational Diseases 2014;32(2):126-127
7.Discussion on standardised induction training for rehabilitation professionals in community health services
Yongxi HUANG ; Weijin ZHOU ; Zhiqiang JIAO ; Zhixin CHENG ; Suyan PAN ; Ying LIU ; Chuntie CHEN ; Maobin WANG
Chinese Journal of Rehabilitation Theory and Practice 2006;12(12):1014-1016
把社区康复纳入社区卫生服务是“人人享有康复服务”的重要保证,而社区卫生服务康复专业人员的规范化上岗培训是提高社区康复服务质量和可持续发展的关键。北京市在社区卫生服务康复专业人员规范化上岗培训方面已取得一定经验,要搞好此项工作,涉及培训的目的和职称、指导思想、师资队伍、培训基地、组织领导等方面的问题。
8.Causes and clinical features of 20 patients with hemolytic uremic syndrome
Wen ZHANG ; Hao SHI ; Hong REN ; Xiao LI ; Pingyan SHEN ; Yaowen XU ; Yongxi CHEN ; Xiaonong CHEN ; Ping ZHU ; Nan CHEN
Chinese Journal of Nephrology 2008;24(9):627-631
Objective To analyze the causes and clinical features of 20 patients with hemolytic uremic syndrome (HUS) in order to improve the prognosis. Methods Twenty patients with HUS hospitalized in our department during July 1998 to December 2004 were enrolled in this study. The etiology, clinical features, individualized therapy and prognosis were retrospectively analyzed. Results These 20 HUS patients (18 HUS patients complicated with ARF) accounted for 2.48% of total patients with acute renal failure (ARF) in our hospital. There were 16 females and 4 males with mean age of (49.11±19.85) years. Five patients were idiopathic HUS and the other 15 were secondary HUS (10 SLE-associated HUS, 2 pregnancy-associated HUS, 1 APS-associated HUS, 1 renal arterioles sclerosis-associated HUS and 1 drug-associated HUS). Eighteen cases had ARF and 15 had nephrotic syndrome. Hypertension was found in 17 patients, among them 4 had malignant hypertension. Twelve patients had gross hematuria and the other 8 had microscopic hematuria. Diarrhea was found only in 1 patient. At onset, mean serum creatinine was (504.40±381.10) μmol/L and 24-h proteinuria was (5.0±2.6) g. Renal biopsy was pedormed in 16 patients. Fourteen patients received hemopurification therapy: 2 patients plasma exchange (PE); 8 patients PE combined with CVVHDF and /or HD; 4 patients CVVHDF and HD. Seven cases were treated with intravenous immunoglobulin (IVIg). Patients with SLE-associated HUS received the corticosteroids and immunosuppressants. Low or middle dosage of corticosteroids( 10-40 mg/d) was administered in patients with idiopathic HUS. For patients with APS, low molecular weight heparin was used. HUS patients were followed-up for average (46.0±32.8) months. During follow-up, 4 patients died, 11 recovered from renal insufficiency, 4 progressed to end stage renal failure of whom 2 depended on dialysis and 1 lost. The survival rates of SLE-associated HUS and none-SLE-associated HUS were 70% and 90%, and renal survival rates were 50% and 60% respectively, which were not significantly different between these two groups. Conclusions Most of the patients are secondary HUS. SLE-associated HUS is the main type of secondary HUS. The prognosis of SLE-associated HUS is poor. PE and IVIg are main therapy. Low dosage of corticosteroids can reduce relapse of HUS. Immunosuppressants can improve the prognosis.
9.A comparative study on the effect of BCG-PSN and thymopeptides on T-lymphocyte subsets of normal and immunosuppressed mice.
Yunhua DENG ; Yingling CHEN ; Xingping CHEN ; Yongxi LI ; Liyi ZHOU
Journal of Huazhong University of Science and Technology (Medical Sciences) 2003;23(4):339-347
To compare the effects of polysaccharide nucleic acid fraction of bacillus calmette guerin (BCG-PSN) and thymopeptides on T-lymphocytes of normal and immunosuppressed mice, CD4+ and CD8+ T-lymphocyte subsets of single nucleic cell in thymus, spleen and peripheral blood were detected successively by flow cytometry after application of BCG-PSN and thymopeptides. Meanwhile, CD4+/CD8+ ratio was also calculated. The results showed that both BCG-PSN and thymopeptides could decrease the proportion of CD4+ CD8+ T-lymphocyte subsets in the thymus, at the same time increase CD4+ T-lymphocyte, CD8+ T-lymphocyte proportion in the three tissues. The fluctuation in amplitude was greater in thymopeptides group than that in BCG-PSN group. It is concluded that acting location of thymopeptides is in thymus, its stimulating action is stronger than that of BCG-PSN, while BCG-PSN not only accelerates the differentiation in thymus, but also has some direct stimulation to peripheral CD4+ T-lymphocytes, and can maintain CD4+/CD8+ ratio within normal range. So, BCG-PSN is safer.
Adjuvants, Immunologic
;
pharmacology
;
Animals
;
Female
;
Immunocompromised Host
;
Male
;
Mice
;
Mycobacterium bovis
;
chemistry
;
Nucleic Acids
;
pharmacology
;
Peptide Fragments
;
pharmacology
;
Polysaccharides, Bacterial
;
pharmacology
;
T-Lymphocyte Subsets
;
drug effects
;
Thymus Gland
;
chemistry
10.The MRI diagnosis of infant brachial plexus injury
Yan ZHANG ; Wenjun CHEN ; Xiaoli WANG ; Huang HUANG ; Yongxi LIU ; Yihui ZENG ; Kuiming JIANG
Journal of Practical Radiology 2018;34(5):749-752
Objective To evaluate the value of MRI in diagnosis of brachial plexus injury in infants.Methods MRI manifestations and electromyography(EMG) data of 14 cases were analyzed retrospectively.Kappa statistic was used to analyze the consistency between the two methods.Results Pure preganglionic injuries were found in 5 cases,preganglionic and postganglionic combined injuries occurred in 9 cases.MRI features of preganglionic injuries include:absence of roots in spinal canal (7 cases),nerve root enlargement (1 case),nerve sleeve dilation (3 cases),pseudomeningoceles (5 cases) and the dura thickening (3 cases).MRI features of postganglionic injuries include:trunk thickening with hyperintensity in STIR images(14 cases),adjacent structure disorder and edema (1 cases).The consistency of MRI and EMG was good (κ=0.752,P<0.05).Conclusion MRI is an efficient method for demonstrating preganglionic and postganglionic injuries,and can provide reliable information for early diagnosis and treatment for brachial plexus injury in infants.