The concept of biochip is originated in 1980s, it can also be called micro-array technology. Biochip is a new and sophisticated technique developed by bio-science and micro-electronics. The birth of biochip technique is a great revolution in modern biology area. Nowadays, the biochip technique has been widely used in many fields, eg, spectrum analysis of gene expression, diagnosis of diseases, drug selection, tumor markers, detection of manifold kinds of viruses, etc. Based on its several characteristics such as high efficiency, sensitivity, economy, parallel, automation, gene-chip technique will be a novel modern diagnosis technique. In this article, the usages of biochip technique in preventive medicine fields including environmental health, virus detection, occupational hygiene, nutrition and food hygiene, were discussed.

The study and discussion of the establishment,continuance,and termination of physician-patient relationship from a legal point of view is of great significance for establishing a harmonious physician-patient relationship,standardizing the rights and duties on both sides,and regulating doctors' clinical practice.The physician-patient relationship is a special type of contractual relationship,whose establishment needs the patients' requirement,doctors' commitment,and the consistence and acknowledgement expressed or implied by both sides.But the patients with acute diseases or dangerously ill or the patients with Class A infectious diseases are exceptions.In the physician-patient relationship,patients enjoy more rights and doctors assume more duties.In the continuance of physician-patient relationship,principles of the bilateral equality and negotiated consensus should be followed.The physician-patient relationship should be terminated when the doctors have fulfilled their duties or the occurrence of statutory causes.

Objective To explore the risk factors of progressive cerebral infraction.Methods 112 cases with progressive cerebral infarction and 112 cases of acute cerebral infarction were selected.Patients were observed and recorded blood pressure,temperature,WBC,cholesterol,GLU and fibrinogen.Results The differences between blood pressure,temperature,WBC,cholesterol,GLU,fibrinogen and artery stenosis were statistically significant.Conclusion Infections,systolic blood pressure decreased,hyperglycemia,hyperlipidemia,and large artery stenosis were related to progressive cerebral infarction.

BACKGROUND: The increase of leukocyte and blood glucose levels is a kind of stress reaction when acute cerebrovascular disease occurs, it often occurs at the early clinical stage of acute cerebrovascular disease.OBJECTIVE: To observe the levels of leukocyte and blood glucose at the early stage of acute cerebrovascular disease, and investigate their correlation with recent prognosis.DESIGN: A prognostic analysis based on diagnosis taken patients as observational subjects.SETTING: Qingtian County People's Hospital.PARTICIPANTS: Totally 235 inpatients with acute cerebrovascular disease aged (63.13±9.55) years were selected from Qingtian County People's Hospital between May 2000 and May 2002, including 90 cases of cerebral hemorrhage (52 males and 38 females) aged (63.13±9.55) years and 145 cases of cerebral infarction (73 males and 72 females) aged (71.22±8.00) years. The disease course ≤ 1 month. According to the number of leukocytes, the patients were divided into normal leukocyte group (n=167) and increased leukocyte group (n=68). According to the level of blood glucose, they were divided into normal blood glucose group (n=146) and hyperglycemia group (n=89). The neurologic deficit score (NDS) of 0-15 points was taken as mild, 16-30 as moderate and 31-45as severe.METHODS: All the patients received cranial CT examination immediately after admission, and fasting venous blood samples were drawn on the next morning to detect the levels of leukocyte and fasting blood glucose. The x2 test was applied.MAIN OUTCOME MEASURES: ① Comparison of age: It was obviously higher in the cerebral infarction group than in the cerebral hemorrhage group [(71.22±8.00), (63.13±9.55) years, t=6.90, P ＜ 0.001]. ② Comparison of the percentage of disease types: In the increased leukocyte group,the percentage of cerebral hemorrhage was obviously higher than that of cerebral infarction (66%, 34%, x2=31.47, P ＜ 0.005). In the hyperglycemia group, the percentage of cerebral hemorrhage was higher than that of cerebral infarction (51%, 49%, x2=9.12, P ＜ 0.005). ③ Comparison of the percentage of severe neurological impairment: It was obviously higher in the increased leukocyte group than in the normal leukocyte group (26%, 6%,x2=21.12, P ＜ 0.005), also markedly higher in the hyperglycemia group than in the normal blood glucose group (27%, 5%, x2=21.70, P ＜ 0.005).④ Comparison of the percentage of death: It was obviously higher in the increased leukocyte group than in the normal leukocyte group (31%, 6%,x2=26.15, P ＜ 0.005), also markedly higher in the hyperglycemia group than in the normal blood glucose group (26%, 5%, x2=20.02, P ＜ 0.005). CONCLUSION: Both the increase of leukocyte and blood glucose can damage the brain tissue, and can be used to judge the disease condition of patients with acute cerebrovascular disease, estimate the prognosis and guide the treatment.

Objective To analyze the CT features of mycoplasma pneumonia(MP)in children,and to provide the reference for clinical diagnosis and treatment.Methods The clincal data of 125 cases with MP were analyzed retrospectively.Results There were 73 cases of unilateral lesions in the case of the right side of the lesion,and the left side of the lesion in 26 cases.The data showed that the incidence of left lesions was significantly less than the incidence of the right side of the lesion.The main manifestations of CT were large real changes shadow,patchy or nodular shadow,thickening of the bronchial vascular bundle,hilar lymph node enlargement,atelectasis,cavity,pleural effusion,and most of them were common.Conclusion The incidence of MP in children is more common in the lower right lung,and the imaging changes are diverse,but the most common is the large real change shadow,and improving the chest CT examination is helpful to the diagnosis of MP.

Objective To investigate efficacy in Alpmstadil and Shenkang on regulating renal function and lipids in patients with chronic renal failure (CRF).Methods Ninety-eight cases of CRF patients were collected for the study,and 1:1 randomized,double-blind experiment grouping principles were used in the current study.All cases were divided into study group and the control group (49 cases of each group).The two group were given the high quality low protein,low fat low salt diet,inftion control,diuretic swelling,correct acidosis,buck,correction of electrolyte imbalance and other conventional treatment.The patients in study group on the basis of conventional therapy were given 20 g Alprostadil injection + 100 ml saline,intravenous infusion of 1times/d,and 100 ml Shenkang injection +300 ml 5％ glucose the solution,intravenous infusion of 1 times/d.While patients of control grrup on the basis of conventional therapy plus 200 mg Ligustrazine + 250 ml 5％Glucose Injection,intravenous infusion of 1times/d.Two groups were treated for 14 d.Before and after treatment,renal function,lipid metabolism,adverse drug reactions were observed and determined.Results There was no significant difference in term of renal function between two groups before treatment(P ＞ 0.05).After treatment,the levels of serum creatinine,blood urea nitrogen,24 h-Urine protein and creatinine clearance(Ccr) in study group were (313.7 ±66.2) mmol/L,(15.3 ±2.9) mmol/L,(1.24 ±0.45) g/24 h and (35.7 ±5.4) mmol/L respectively,significant different from those in control group ((358.4 ± 53.9) mmol/L,(18.4 ± 3.0) mmol/L,(1.57 ±0.39) g/24 h and (28.4 ±4.3) mmol/L;t =3.754,7.864,5.873,4.312,P ＜0.05).There was no significant difference in term of blood fat between two groups before treatment (P ＞ 0.05).In study group,the levels of glycerin trilaurate,cholesterol total,high density hpoprotein cholesterol,low density lipoprotein cholesterol were significantly different than those in the control group ((2.12 ± 1.26) mmol/L vs.(2.32 ± 1.34) mmol/L,(4.91 ± 1.43) mmol/vs.(5.35 ± 1.31) mmol/L,(1.39 ± 0.43) mmol/L vs.(1.23±0.37) mmol/L,(2.39 ± 0.68) mmol/L vs.(2.73 ± 0.88) mmol/L; t =3.876,4.661,5.387,8.543 ; P ＜ 0.05) after treatment.There were no adverse drug reactions in both groups.Conclusion Combined usage of alprostadil and shenkang injection could significantly improve the patient's renal function,lipid metabolism disorders and shows no significant adverse reactions,suggesting that it is worthy of clinical application and promotion.

To study the efficacy of CAG regimen used in 35 cases of newly diagnosed acute myeloid leukemia in elder-ly patients. Methods:Totally 35 cases of elderly patients with acute myeloid leukemia were randomly divided into two groups. The pa-tients in the control group (15 cases) were treated with standard chemotherapy treatment, while the CAG regimen group (20 cases) was treated with CAG regimen (cytarabine, aclarubicin and granulocyte colony-stimulating factor). After one treatment course, the chemotherapy efficacy was observed, and the neutrophil and platelet recovery time and the incidence of adverse reactions in the two groups were compared. Results:The treatment efficacy of the CAG regimen group was 90%, which was higher than that of the control group (60%, P<0. 05). The neutrophil and platelet recovery time of the CAG regimen group was significantly shorter than that of the control group (P<0. 05). The incidence of adverse reactions in CAG regimen group was much lower than that of the control group(P<0. 05). Conclusion:The efficacy of CAG regimen used in the 35 cases of newly diagnosed acute myeloid leukemia in elderly pa-tients is promising, which can effectively control the progression of the disease and accelerate blood cell recovery with high safety.

Objective To investigate therapeutic effects of CT-guided percutaneous minimally invasive treatment by using Herbert screws for wrist scaphoid fracture . Methods From September 2010 to October 2012, 21 patients with scaphoid fracture were treated by internal fixation by using Herbert screws under the guidance of CT .The results of the procedure were assessed in terms of osseous union , pain extent , grip strength , and active motion of the wrist .Postoperative outcomes were evaluated by wrist standard scores based on patients’ perception of functions. Results The operation time was 25 -55 min (mean, 35.3 min).The intraoperative blood loss was 8-40 ml (mean, 18.6 ml).Fracture fragments were cured with bone union , with an average healing time of 10.4 weeks (range, 8-17 weeks).All the 21 cases were followed up for 8 -13 months (mean, 9.8 months).The VAS scores at the last follow-up was (1.2 ±0.8) points, which was significantly lower than that before the operation (5.3 ±1.4) points (t=11.785, P=0.000).The grip strength was (39.6 ±3.5) kg, which was significantly higher than that preoperatively [(19.4 ± 2.4) kg, t=-21.624, P =0.000].The Angle of wrist flexion and extension was 115.2°±7.4°, significantly higher than preoperative measure (89.8°±10.4°, t =-9.103, P =0.000).The wrist ulnar radial deflection angle was 53.7°±5.5°, significantly higher than preoperative one (39.4°±3.4°, t =-10.116, P=0.000).At the last follow-up assessment of wrist functions, excellent result was recorded in 18 cases, and good in 3 cases, with a good-or-excellent rate of 100%. Conclusion Treatment of scaphoid fracture with Herbert screws under the guidance of CT is a minimally invasive method , bearing advantages of little incision, simple operation performance , and good effects .

Objective To observe the impact of RNA interference-induced ADAR1 down-regulation on cell proliferation of liver cancer.Methods Small interfering RNA (siRNA) was transfected into liver cancer cell line SMMC-7721.ADAR1 expression was detected by RT-PCR and Western blotting.Cell proliferation was determined by methyl thiazol tetrazolium (MTT) assay.Results After transfection for 24,48,and 72 h,ADAR1 mRNA expression was 0.612 ± 0.086,0.264 ± 0.018,0.156 ± 0.063 in experimental group and 1.032 ± 0.107,0.898 ± 0.092,0.968 ± 0.074 in control group,respectively.Experimental group had significantly lower ADAR1 mRNA than the other groups (P ＜ 0.05),and there was no statistically significant difference between control and blank group (P ＞ 0.05).ADAR1 protein relative expression was 0.684 ± 0.079,0.324 ± 0.042,0.145 ± 0.058 in experimental group and 1.002 ± 0.092,0.917 ± 0.068,0.972 ± 0.073 in control group,respectively,which was statistically significant (P ＜ 0.05).After transfection with siRNA,the proliferation ability of SMMC-7721 cells was enormously inhibited (P ＜ 0.05).Conclusion ADAR1 mRNA and protein level could be significantly decreased by specific RNA interference,and cell proliferation in SMMC-7721 cells were also greatly inhibited.

Objective:To observe the effect of “bulk-fill”flowable resin in filling deep wedge-shaped defects.Methods:50 pa-tients with totally 85 pairs of premolars with deep wedge-shaped defects(depth >2 mm)were included.Each pair of the defects was randomly divided into the test group and the control group,the defects were treated by “bulk-fill”flowable resin with single cure tech-nique and conventional flowable resin with incremental layering technique respectively,the tooth sensitive rate 1 week after filling,the filling failure rate,the edge coloring and secondary caries rate 2 years after filling of the 2 groups were compared.Results:There was no significant difference between the 2 groups in the sensitive rate 1 week after filling,the failure rate and the edge coloring and sec-ondary caries rate 2 years after filling(P >0.05).Conclusion:The “bulk-filled”flowable resin using single cure technique can ac-quire the same clinical effect as conventional flowable resin using incremental layering technique in filling deep wedge-shaped defects.