1.Protection of chickens against infectious bronchitis virus with a multivalent DNA vaccine and boosting with an inactivated vaccine.
Fang YAN ; Yujun ZHAO ; Yongting HU ; Jianyang QIU ; Wenxin LEI ; Wenhui JI ; Xuying LI ; Qian WU ; Xiumin SHI ; Zhong LI
Journal of Veterinary Science 2013;14(1):53-60
The protective efficacy of DNA plasmids encoding avian infectious bronchitis virus (IBV) S1, N, or M protein was investigated in chickens. Chickens were inoculated monovalently (with plasmid pVAX1-16S1, pVAX1-16M, or pVAX1-16N alone) or multivalently (combination of the three different plasmids, pVAX1-16S1/M/N). A prime-boost immunization protocol against IBV was developed. Chickens were immunized with the multivalent DNA vaccine twice and then boosted with an inactivated vaccine once. Antibody titers of the chickens immunized with pVAX1-16S1/M/N were much higher than those of the monovalent groups (p < 0.01). A protective rate up to 90% was observed in the pVAX1-16S1/M/N group. The serum antibody titers in the prime-boost birds were significantly higher than those of the multivalent DNA vaccine group (p < 0.01) but not significantly different compared to the inactivated vaccine group at 49 days of age. Additionally, the prime-boost group also showed the highest level of IBV-specific cellular proliferation compared to the monovalent groups (p < 0.01) but no significant difference was found compared to the multivalent DNA vaccine group, and the prime-boost group completely protected from followed viral challenge.
Aging
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Animals
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Antibodies, Viral/blood
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Cell Proliferation
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Chickens
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Coronavirus Infections/prevention & control/*veterinary/virology
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Immunization, Secondary/veterinary
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Infectious bronchitis virus/*immunology
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Poultry Diseases/*prevention & control/virology
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T-Lymphocyte Subsets/cytology/physiology
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Vaccines, DNA/immunology
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Vaccines, Inactivated/immunology
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Viral Vaccines/*immunology
2.Preliminary evidence for the presence of multiple forms of cell death in diabetes cardiomyopathy.
Jinjing WEI ; Yongting ZHAO ; Haihai LIANG ; Weijie DU ; Lihong WANG
Acta Pharmaceutica Sinica B 2022;12(1):1-17
Diabetic mellitus (DM) is a common degenerative chronic metabolic disease often accompanied by severe cardiovascular complications (DCCs) as major causes of death in diabetic patients with diabetic cardiomyopathy (DCM) as the most common DCC. The metabolic disturbance in DCM generates the conditions/substrates and inducers/triggers and activates the signaling molecules and death executioners leading to cardiomyocyte death which accelerates the development of DCM and the degeneration of DCM to heart failure. Various forms of programmed active cell death including apoptosis, pyroptosis, autophagic cell death, autosis, necroptosis, ferroptosis and entosis have been identified and characterized in many types of cardiac disease. Evidence has also been obtained for the presence of multiple forms of cell death in DCM. Most importantly, published animal experiments have demonstrated that suppression of cardiomyocyte death of any forms yields tremendous protective effects on DCM. Herein, we provide the most updated data on the subject of cell death in DCM, critical analysis of published results focusing on the pathophysiological roles of cell death, and pertinent perspectives of future studies.