1.Research progress of minimally invasive surgery for congenital diaphragmatic hernia
Chinese Journal of Applied Clinical Pediatrics 2016;31(11):878-880
Congenital diaphragmatic hernia(CDH) is characterized by a spectrum developmental defects in the diaphragm caused by embryogenesis disorders,and lead to incomplete fusion of elements which results in rise of the diaphragm.With the promotion of antenatal diagnosis,postoperation rehabilitation and neonatal intensive care unite,the mortality of CDH has been remarkably decreased.Nevertheless,the condition is uncommon enough that it has proven to be difficult to conduct prospective studies,leading to the lack of evidence of evidence-based medicine to explore the pathological mechanism and therapies.With the rise of endoscopic technique,the laparoscopy and thoracoscopy are also gradually carried out for the surgical management of CDH.Now,the minimally invasive surgery for CDH was reviewed.
2.Expression of Nerve Growth Factor (NGF) in Mouse Testes at Different Developmental Stages
Yongting HU ; Wenhua CHEN ; Ying ZHANG ; Xiangting NIE
Acta Laboratorium Animalis Scientia Sinica 2010;18(1):28-32,封3
Objective To study the location and the expression of nerve growth factor(NGF)in mouse testes at different developmental stages.Methods The testes were taken out from mice at different developmental stages,respectively.Parts of the testis tissue were used to extract total RNA and real-time PCR was used to detect the expression of NGF.The rest tissues were fixed and embedded in paraffin.SABC immunohistochemical staining was used to observe the location of NGF in the testes. Results Reat-time PCR results showed that NGF mRNA could be detected in the testes of mouse at postnatal 1 st week and its expression level increased with the development,and reached the highest point at 3rd week.After 5 weeks it lowered down to a stable level.Immunohistochemistry showed that NGF expressed in the mouse testes at all developmental stages.At postnatal 1 st week immunopositive reaction of NGF was detected mainly in sustentacular cells and the spermatogonia also showed positive staining.NGF positive staining in the testes was observed in interstitial cells,spermatogenetic cells,sustentacular cells and Leydig cells at 3rd week.After the postnatal 5th week,NGF-positive immunostaining was also detected in intersitial cells and spermatogenetic cells,but the intensity of reaction was weaker than that at 1 st and 3rd weeks.Conclusion The expression levels of NGF in mouse testes show regular changes and the expression profile and location of NGF in the testes are different at different developmental stages.
3.Effect of intermittent pneumatic pressure pump on the formation of deep venous thrombosis of lower extremity in elderly patients undergoing thoracotomy
Lixia WANG ; Yongting WEI ; Yanwen ZHANG ; Tinghui CHEN ; Xiuhong WU
Modern Clinical Nursing 2015;(2):41-43
Objective To investigate the effect of intermittent pneumatic pressure pump on the formation of deep venous thrombosis of lower extremity in elderly patients undergoing thoracotomy. Methods Fifty patients undergoing thoracic surgery were managed with intermittent pressure inflation pump besides general preventive measures. The pre-and postoperative blood flow in the lower extremity and incidences of deep venous thrombosis were compared. Result The differences in blood flow in the lower extremity and incidences of deep venous thrombosis were not statistically significant (P>0.05). Conclusion Intermittent pneumatic pressure pump can promote pulsatile blood flow in the lower extremity so as to effectively prevent formation of deep venous thrombosis in elderly patients after thoracotomy.
4.Single-site intracorporeal purse-string stitching versus single-port extracorporeal knotting for laparoscopic inguinal inner ring closure in children: a comparative study
Xuelai LIU ; Chuan FEI ; Yongting ZHANG ; Chi SUN ; Yanbin FANG ; Xiaofeng YANG ; Lin LIU ; Suolin LI
Chinese Journal of General Surgery 2017;32(4):328-331
Objective To compare the surgical and functional outcomes of single-site (transumbilical two-port) intracorporeal purse-suturing (IP) and single-port extracorporeal knotting (EK) for laparoscopic pediatric inguinal hernia (PIH) repair.Methods Between June 2008 and December 2014,358 PIH children underwent lapamscopic inguinal herniorrhaphy,including 126 treated by single-site intracorporeal purse string stitching using a needle-holder (IP group),and 232 by single-port extracorporeal knotting using an inner two-hook needle with preperitoneal hydrodissection (EK group).Results In all patients laparoscopic procedures were completed successfully without conversion.The operating time in IP group was significantly longer than that in EK group [unilateral:(20.4 ± 2.1) min vs.(9.4 ± 1.3) min,t=-5.23,P<0.01;bilateral:(31.3 ±2.9) min vs.(15.2±1.7) min,t=-4.22,P<0.01)].The hospital stay were similar between the two groups [(2.35 ±0.25) d vs.(2.38 ±0.18) d,t =-0.062,P > 0.05].Five cases had intraoperative hematoma in the IP group while none in the EK group.One each suffered from recurrence in IP group and EK group.Three postoperative hydroceles were seen in IP group and one in EK group.Subcutaneous knot granulomas were seen in two in EK group.Conclusions Both IP and EK laparoscopic procedures are safe and feasible.With the assistance of preperitoneal hydrodissection technique,single-port laparoscopic EK herniorraphy is superior to single-site IP repair in easy performance and shorter operation time.
5.Visualization Analysis on Literature about TCM Treatment of Tic Disorder from 2000 to 2022
Xinyue XIE ; Rongyi ZHOU ; Lilu ZHU ; Shen'ao DING ; Yongting ZHANG ; Qirong WANG
Chinese Journal of Information on Traditional Chinese Medicine 2024;31(1):36-43
Objective To investigate the current status,research hotspots,and emerging trends in the field of TCM treatment for tic disorder(TD);To provide references for relevant research.Methods Literature on the TCM treatment for TD was retrieved from CNKI,VIP,Wanfang Data,Web of Science Core Collection,and PubMed databases from January 1,2000 to May 1,2022.NoteExpress 3.7.0.9296 software was used for literature management.VOSviewer 1.6.18 software was utilized to conduct co-occurrence analysis on author,institution,and keyword information.R 4.1.3 was applied to statistically analyze keyword frequency and time distributions and generate visualizations.Results A total of 1 520 articles were included in the analysis,involving 107 core authors.High-frequency keywords included experience of famous doctors,clinical observation,acupuncture,and ear acupoints.Commonly used Chinese herbal medicines included Acori Tatarinowii Rhizoma and Bombyx Batryticatus.Frequently prescribed TCM formulae included Wendan Decoction,Changpu Yujin Decoction,Yinqiao Powder,and Tianma Gouteng Decoction.Conclusion Current research hotspots in this field focus on experience of famous doctors,the clinical efficacy of TCM interventions for TD,and molecular biological mechanism studies.Emerging research trends include external TCM treatment and comorbidity investigations.
6.Effect of different immunization schedules on diphtheria antibody level among preschool children in Shenzhen
PENG Yuanzhou, HA Yongting, LI Wenhao, HUANG Fang, XIE Xu, CHENG Jinquan, WU Yu, ZHANG Yanwei
Chinese Journal of School Health 2023;44(3):415-418
Objective:
To evaluate the immunity and influencing factors of diphtheria among preschool children in Shenzhen,to provide reference for effective monitoring of diphtheria IgG antibody level in preschool children.
Methods:
Serum samples were collected from 296 preschool children aged 4-6 who were recruited in Shenzhen. The diphtheria antibody titer in serum was determined by enzyme linked immunosorbent assay, and the effect of different immumuzation schedule including types of vaccine and vaccination timing, on the geometric mean concentration (GMC) of diphtheria IgG antibody and antibody positive rate were analyzed.
Results:
The GMC of diphtheria IgG antibody was 0.71 IU/mL, and the positive conversion rate was 33.1%. There were significant differences in antibody GMC and antibody positive conversion rate of diphtheria in different age groups( F/χ 2=11.77, 27.45, P < 0.01 ). The GMC and antibody positive conversion rate showed significant differences by diphtheria antibodies, vaccine types and end dose vaccination intervals( F=49.53, 12.95,11.61, P <0.01). There were statistically significant differences in the positive conversion rate of diphtheria antibodies in children with different types of diphtheria antibodies, vaccine types of diphtheria antibodies, and diphtheria antibodies at the time interval of final vaccination (Fisher exact probability method, P <0.01).
Conclusion
The overall positive conversion rate of diphtheria antibody in preschool children in Shenzhen is high. Timely completion of full diphtheria vaccination can improve the antibody level and plays a better role in protecting preschool children.
7.Research progress on the pathogenesis of attention deficit hyperactivity disorder based on the theory of Dopamine deficits
Rongyi ZHOU ; Bingxiang MA ; Yongting ZHANG ; Xinyue XIE ; Chenlei WU ; Xueying DING
Chinese Journal of Applied Clinical Pediatrics 2023;38(8):623-628
Attention deficit hyperactivity disorder (ADHD) is a common neurodevelopmental disorder in childhood, which seriously affects physical and mental health in children.Its etiology and pathogenesis are complex and have not been fully elucidated.Currently, the theory of Dopamine (DA) deficits has been widely recognized and studied in the international academic community.The DA system is considered as the key to the pathogenesis of ADHD.The causes of DA deficits are complex.In addition to the well-established reuptake disorder caused by abnormal DA transporter function, DA deficits are also associated with the activation of DA vesicle cycle enzymatic inactivation, vesicle transport dysfunction, and receptor dysfunction, which are of great significance in analyzing disease pathogenesis and drug development.This article reviews the research on the causes of DA deficits proposed in recent years based on the theory of DA deficits, aiming to provide ideas and references for the research on the pathogenesis of ADHD in China.
8.Effects and mechanism of Glycyrrhiza inflata polysaccharides on the maturation and anti-tumor effect of dendritic cell
Xiaokelati NADIREMU ; Yongting YU ; Yongqiang LU ; Zhaoxi ZHOU ; Lili ZHANG ; Yuanyuan CONG
China Pharmacy 2024;35(20):2453-2459
OBJECTIVE To investigate the effects and mechanism of Glycyrrhiza inflata polysaccharides (GiP) and GiP-B1 on the maturation and anti-tumor effect of dendritic cell (DC). METHODS The immature DC (imDC) of hepatocellular carcinoma cell H22 tumor-bearing mice cultured in vitro were divided into control group, tumor necrosis factor-α (TNF-α) group, GiP group, and GiP-B1 group. The viability, positive expressions of surface markers (CD11c, CD80, CD86, MHC-Ⅱ), the levels of interleukin-12p70 (IL-12p70) and IL-4 in mature DC (mDC) of tumor-bearing mice were detected. mDC and CD4+T lymphocytes were co-cultured to generate CD4-cytotoxic T cell (CD4-CTL); stimulation index, the levels of IL-12p70, interferon-γ (IFN-γ), IL-4 and IL-10, the killing activity of CD4-CTL to H22 cell were detected. mRNA expressions of IL-12, IL-12 receptor (IL-12R), signal transducer and activator of transcription-4 (STAT-4), as well as the protein expression of IL-12 receptor β2 (IL-12Rβ2), phosphorylation levels of nuclear factor-kappa B (NF- κB) p65 and STAT-4 proteins in mDC were detected after co-culture. RESULTS Compared with the control group, the viability of mDC, the positive expressions of MHC-Ⅱ, and the levels of IL-12p70 and IL-4 were increased significantly in GiP group and GiP-B1 group (P<0.05). The positive expressions of CD11c, CD80 and CD86 showed an increasing trend, but the differences were not statistically significant (P>0.05). After co-culturing, the stimulation index, the levels of IL-12p70 and IFN-γ were significantly increased (P<0.05), while the levels of IL-4 and IL-10 (except for the GiP group) were significantly decreased (P<0.05); the cytotoxicity against H22 cells was significantly enhanced (P<0.05). mRNA expressions of IL-12 and IL-12R (except for GiP group) and STAT-4, protein expression of IL-12Rβ2 as well as phosphorylation levels of NF-κB p65 and STAT-4 protein were increased significantly in mDC (P<0.05). CONCLUSIONS GiP and GiP-B1 have a good promoting effect on the maturation of DC in tumor-bearing mice, effectively stimulate CD4+T cell proliferation, enhance the anti-tumor activity of CD4-CTL,and its mechanism may be related to activating IL-12/NF-κB/ STAT-4 signaling pathway.
9.CD146 is essential for PDGFRβ-induced pericyte recruitment.
Jianan CHEN ; Yongting LUO ; Hongxin HUANG ; Shuilong WU ; Jing FENG ; Jingjing ZHANG ; Xiyun YAN
Protein & Cell 2018;9(8):743-747
10.CD146: a potential therapeutic target for systemic sclerosis.
Lingling ZHANG ; Yongting LUO ; Xiao TENG ; Zhenzhen WU ; Mengtao LI ; Dong XU ; Qian WANG ; Fei WANG ; Jing FENG ; Xiaofeng ZENG ; Xiyun YAN
Protein & Cell 2018;9(12):1050-1054