ObjectiveTo observe the effects of medical membrane of combination of the traditional Chinese medicine with the western medicine on white lesion of the vulva.Methods141 cases of white lesion of the vulva were put on vulvar pathological area with the medical membrane, twice a day.ResultsThe total effective rate was 98.5%.ConclusionThe effect of medical membrane of combining the traditional Chinese medicine with the western medicine is very satisfying.

Objectives To investigate the effect of cerebrespinal fluid replacement using three- way pipe on subarachnoid hemorrhage and the nursing points were summarized. Methods 80 subarachnoid hemorrthage patients were divided into the treatment group and the control group with 40 patients in each group. The control group received traditional medical treatment and routine nursing. The treatment group received cerebrospinal fluid replacement.The incidence of complication,death and clinical effect were com-pared between the two groups and the data under χ2 test and t test. Results Each index in the treatment group was better than that of the control group. Conclusions Intensified nursing after cerebrospinal fluid replacement can improve treatment effect, alleviate prognosis and supply reference for future nursing work for this disease.

Objective To study the location and level of relaxin receptor in placenta tissues of preeclampsia and normal pregnancy,and the relationship of relaxin receptor with pre-eclampsia.Methods Twenty-six placenta tissue samples from pregnant women with severe preeclampsia(study group),and 20samples from normal pregnancies(control group)were obtained.We detected the expression of relaxin receptor by immunohistochemistry and the expression of relaxin receptor mRNA by RT-PCR.Results In the placenta of control and preeclampsia groups,the leucine-rich repeat-containing G protein-coupled receptor(LGR7)was positively expressed.Relaxin receptor was located in the membrane of trophoblast cells.There were both strong signals on cytotrophoblastic cells and syneytiotrophoblast.The level of relaxin receptor in the control group was 0.912 ±0.003.and 0.625±0.037 in the preeclampsia group.Thedifference between the two groups was obvious(P＜0.01).In the control group,the level of relaxin receptor mRNA was 0.776±0.021;in the preeclampsia group,it was 0.393±0.075.The difference was obvious(P＜0.01).Conclusion Decrease in the expression of relaxin receptor at placenta is related with the occurrence and development of preeclampsia.

Objective To explore the clinical significance of plasma LP (a) ,Hcy ,hs-CRP and cTnI concentrations in patients with AMI .Methods Plasma LP(a) ,Hcy ,hs-CRP and cTnI concentrations of 162 patients with AMI and 165 healthy people recrui-ted as control group were determined .Immunofluorescent dry quantitative method and electro-chemiluminescence immunoassay were used in the determination ,and statistical analysis were used in data comparison .Results Before treatment ,the plasma LP(a) , Hcy ,hs-CRP and cTnI concentrations in 162 AMI patients were significantly higher than those in control group (P<0 .01) .After treatment ,the plasma hs-CRP concentrations in 97 improved patients returned to normal (t=1 .586 ,P>0 .05) ,but the plasma cTnI concentrations significantly decreased (t=3 .322 ,P<0 .01) .The plasma Hcy ,hs-CRP and cTnI concentrations in 36 died patients were significantly higher(P<0 .01) .Conclusion Plasma LP(a) ,Hcy ,hs-CRP and cTnI determination were valuable for the diagno-sis of AMI patients ,the most valuable indicators for patient prognosis and follow-up were plasma hs-CRP and cTnI .

[Objective]To investigate the pathogenesis,pathology,diagnosis and therapy of hyponatremia after acute spinal cord injury.[Method]Fifteen in-patients with hyponatremia after acute spinal cord injury from 2004 to 2006 were retrospectively analyzed.[Result]Serum sodium was lower than 130 mmol/L in all cases.Urine sodiumcanned was from 40 to 68 mmol/L and urine osmotic pressure was from 420 to 980 mmol/L.After limitation of water intake and appropriate salt intake,12 patients recovered at 2 to 3 weeks.Because of fever and avoiding limitation of water intake,the other 3 patients recovered slowly.[Conclusion]The more serious the acute spinal cord injury,the higher the frequency of hyponatremia;syndrome of inappropriate antidiuretic hormone secretion(SIADH) is its primary cause: appropriate measures should be taken to correct the hyponatremia according to the findings of serum sodium and urine osmotic pressure.

Mycoplasma ovipneumoniae (Mo) is difficult to culture, resulting in many difficulties in related research and application. Since nucleotide metabolism is a basic metabolism affects growth, this study conducted a “point-to-point” comparison of the corresponding growth phases between the Mo NM151 strain and theMycoplasma mycoides subsp. capri (Mmc) PG3 strain.The results showed that the largest difference in nucleotide metabolism was found in the stationary phase. Nucleotide synthesis in PG3 was mostly de novo, while nucleotide synthesis in NM151 was primarily based on salvage synthesis. Compared with PG3, the missing reactions of NM151 referred to the synthesis of deoxythymine monophosphate. We proposed and validated a culture medium with added serine to fill this gap and prolong the stationary phase of NM151. This solved the problem of the fast death of Mo, which is significant for related research and application.

Objective To explore the clinical significance on power spectra assay of heart rate (HRPSA) to cardiovascular autonomic nervous system function in patients with interictal epilepsy.Methods HRPSA of 48 patients with epilepsy and 31 healthy controls were assessed in the study. their heart rate variability(HRV) was analysed.Results HRV was significantly reduced in patients with interictal epilepsy, especially in the patients received carbamazepine treatment.Conclusion The cardiovascular autonomic nervous system function exists disturbance in patients with interictal epilepsy,especially in the patients received carbamazepine treatment.

Objective To design a new automatic rinse assemblage for cage of medical laboratory animal in order to improve the work efficiency and safety of laboratory animal center.Methods The practices of laboratory animal were analyzed retrospectively and the animal experiment were investigated.Results The new automatic rinse assemblage for cage of medical laboratory animal was designed and its main function and operation methods were explained.Conclusion The automatic rinse assemblage for cage of medical laboratory animal has advantages of advanced function and convenient operation,and it has good perspective for medical laboratory animal management.

Objective:This study aimed to develop a Chinese version of Quality of life in Epilepsy Inventory(QOLIE-89) and to confirm its psychometric properties.Methods:The original English version QOLIE-89 was adapted to Chinese language through a translation back-translation procedure. The assessment included 204 patients with epilepsy .The test-retest and internal consistency reliabilities, criterion validity and discriminative validity were assessed.Results:The test-retest reliability (Pearson's correlation coefficient) for Chinese version QOLIE-89 was 0.63-0.95 and internal consistency reliability (Cronbach's alpha) was 0.76-0.92. The confirmatory factor analysis indicated that the Chinese version of QOLIE-89 was composed of three dimensions(CMIN/DF=2.17,GFI=0.98, AGFI=0.90, CFI=0.98,RMSEA=0.061), which were physiology, psychology and social function that including 17 factors obtained by exploration analysis ,which can explained 71.91% of total variance. Spearman's rank correlations between Chinese version QOLIE-89 total score and QOLIE-31 total score was 0.96 and domain scores were from 0.86-1.00.QOLIE-89 items could be discriminated well between patients according to epileptic seizures severity and antiepileptic drug side effects.Conclusion:The psychometric properties of the Chinese version QOLIE-89 inventory are fairly good and similar to the American version and can be applied to assess quality of life for adult patients with epilepsy in China.

Objective To detect the doubling time of airway smooth muscle cells of human and rat by the xCELLigence in-strument,a real time cellular analyzer.Methods The airway smooth muscle cells were separated by collagenase-pancreatin digestion from the rat airway.Then,added to the different holes of E-plate of xCELLigence instrument with the planting destiny of 3 000 cells/well.and so were the human airway smooth muscle cells.The E-plate was then placed on the xCELLi-gence instrument to monitor cell proliferation for 100 hours to calculate the doubling time by using the RTCA Software Package 2.0 software.Results The doubling time of human airway smooth muscle cell calculated by the real time cellular analyzer was 23.96±0.47 h,which was consistent with the data provided by the reference.The doubling time of rat airway smooth muscle cell was 18.62±0.15 h,and the computational process was simple,time-saving and also effective.Conclusion The xCELLigence instrument can be used to calculate doubling time of airway smooth muscle cells of human and rat, which provides experimental methods and reference data for the basic respiratory disease research.