A novel 2-( 2’-hydroxy-5’-chlorophenyl )-6-chloro-4 ( 3 H )-quinazolinone ( ELF-97 )-based fluorescent probe (P1) for hydrogen peroxide was prepared from 5-chlorosalicylaldehyde, 4-(bromomethyl) phenylboronic acid and 2-amino-5-chlorobenzamide, and its structure was characterized by 1 H NMR, 13 C NMR and HRMS. Weak fluorescence intensity was observed at 425nm when the solution of probe P1 was excited by 360 nm UV light. After addition of H2 O2 , however, emission peak at 425 nm disappeared while strong peak emission at 515 nm appeared with the same excitation wavelength ( 360 nm ) . The fluorescence intensity at 515 nm was dependent on the concentration of H2 O2 in the linear response range of 5-45 μmol/L. The detection limit of H2O2 was 0. 1 μmol/L (S/N=3) and the recovery rates of added H2O2 in milk were in the range of 94 . 0%-106 . 0%. Probe P1 was potential to become a useful tool for rapid detection of hydrogen peroxide.

Objective To study the influence of different moment-to-force ratios (M/F) and forces on canine displacement and periodontal ligament (PDL) stress. Methods A three-dimensional (3D) mandibular model was established based on CT images. The orthodontic force systems without a moment and with various M/F were applied to the canine for numerical calculation of its translation. An optimum force system to realize canine translation was validated by analyzing translation displacement of the canine in each case. Results The PDL stress increased with an increase in force magnitude in all cases. The PDL stress first decreased with an increase in the M/F before the M/F reached the optimum value (M/F=10.1 mm), and then increased with an increase in the M/F. The initial displacement of the canine was inclined movement, which was most close to translation when the precise optimum M/F was applied. Conclusions The canine movement requires an appropriate force to prevent root absorption, whereas the translation movement requires an optimal M/F value which is within the physiological range. Understanding the influence of force and moment on tooth translation can help to apply more reasonable orthodontic force system and design more reasonable orthodontic device.

Objective We reported the rehabilitative nursing points of patients with pectoralis minor muscle transplantation for recovery of thumb oppositional function. Methods We selected 10 patients who underwent pectoralis minor muscle transplantation for recovery of thumb oppositional function and gave them perfect preoperative examination and psychological nursing. We also implemented rigorous monitoring postoperation and auxiliary electric stimulation and functional exercises. The emphasis was put on the exercise of thumb oppositional function. Results The thumb oppositional function of all the ten patients recovered. The myodynamia reached level 4 and the shape of thenar was satisfying after 6 to 12 months of follow-up visit. Conclusions Sufficient preoperative psychological nursing and effective postoperative rehabilitative treatment had pivotal significance for the success of operation and the recovery of thumb oppositional function.

Objective To explore the effect of quality control circle (QCC) in reducing the health examination report errors. Methods QCC was founded, activity themes were selected, activity schedule was planned, the reasons of health examination report errors were analyzed, goal setting, countermeasures were planned and implemented jointly by circle members. Totally 11 738 reports of health checkup were selected and analyzed the errors were analyzed during the activity, 6 892 were male, 4 846 were female, their average age was(38.01 ± 11.31)years, 5 000 reports were made before improvement, 5 120 reports during improvement, 1 618 reports after improvement and compared the report error rates were compared. Evaluation of QCC activity results and tracking effect were evaluated. Results Error rate of health checkup report from 17.80‰ decreased to 7.23‰ during improvement, dropped to 2.48‰ after improvement. The rate of standard was 141.9%, progress rate was 86.1%. Tracking results for 8 months, error rate remained below the target value of 3.40‰although error rate roise to 5.25‰in August 2016, the effect was good. The additional benefit, working efficiency of the doctors was improved; Average time for each written report was reduced to 4 minutes from 5 minutes;Saved about 1 hours per person a day to review the accuracy of the report and learning; Formed“communication model between clinical departments”;Established the error registration system for the report has been distributed;Similar error of ECG department has been avoided. Conclusions Application of QCC not only reduced the error rate of the health checkup report, but also ensured quality, objective, and accurate physical examination report; activities not merely help to improve the work efficiency of physician, team's mutual cooperation, and is beneficial to optimize the system of medical institution and avoid the error.

Objective: To explore the effectiveness of exercise combined with nutrition intervention on body mass index (BMI), blood glucose and blood lipid in obese adolescents. Methods: Eighty obese adolescents who participated in a weight-loss training camp were selected as the intervention group, and 50 healthy adolescents were selected as the control group. Adolescents in the intervention group received 8-week weight-loss training and nutritional intervention, while those in the control group maintained their original lifestyle. The changes in body weight, body fat percentage, blood glucose, lipid and inflammatory indicators were compared before and after intervention in both groups. Results: After intervention, weight, BMI, body fat percentage and waisthip ratio of the intervention group were significantly lower than those before intervention (P<0.05). The levels of insulin, HOMA-IR, TG and LDL in the intervention group before intervention were significantly higher than those in the control group (P<0.05). There was no significant difference in blood glucose after intervention (P>0.05). The levels of insulin, HOMA-IR, TG and LDL after intervention were significantly lower than those before intervention (P<0.05). There was no significant difference in HOMA-IR, TG and LDL levels between the intervention and the control group (P>0.05). The levels of TNF-α and hs-CRP in the intervention group before intervention were significantly higher than those in the control group (P<0.05); the levels of TNF-α and hs-CRP in the study group after intervention were significantly lower than those before intervention(P<0.05); the levels of TNF-α and hs-CRP in the control group before and after intervention had no significant changes (P>0.05). Conclusion Exercise combined with nutrition intervention can effectively reduce body fat and body weight, improve insulin resistance, regulate glycolipid metabolism and reduce inflammation in adolescent obese patients.

Objective To study the protective effect of hyperin( Hyp) on the acute liver injury in rats induced by CCl4 . Methods The acute liver injury model was induced by CCl4 . The effect of Hyp on acute liver injury of rat liver histopathology was observed;and the impact of changes of homogenates total superoxide dismutase ( T-SOD) ,glutathione ( GSH) activity and malondialdehyde ( MDA) in liver were de-tected. Results The acute liver injury of tissue induced by CCl4,apparent pathological inflammation and fibrous tissue degeneration and necrosis were observed by HE staining;At the high doses of 60 mg/kg and medium doses of 30 mg/kg treated by Hyp,liver pathology changes was significantly obvious. The T-SOD,GSH activity of liver tissue was significantly increased in the groups of Hyp treatment,MDA content was significantly decreased,which related to the dosages. Conclusion In the rat model of acute liver injury induced by CCl4 . A better therapeu-tic effect of Hyp was shown,the mechanism may be related to its antioxidant activity.

Objective To investigate the chondrogenic feasibility of the human umbilical cord derived mesenchymal stem cells (hUCMSCs)as cartilage tissue engineering seed cells ,type Ⅱ collagen composite glycosaminoglycan scaffold as the cellular carrier and cell‐scaffold complex .Methods The type Ⅱ collagen composite glycosaminoglycan scaffolds was prepared .The pore diameter , porosity and hydrophilia of scaffold materials were observed and measured by electronic microscope .The corresponding histological analysis on the scaffold materials was performed .hUCMSCs of P3 generation were cultured and identified .The hUCMSCs suspen‐sion was inoculated in the type Ⅱ collagen composite glycosaminoglycan scaffold for conducting culture without adding inducer .The samples were taken out after 3 weeks and performed the toluidine blue and safranin O staining ,type Ⅱ collagen immunohistochemi‐cal staining and SEM scanning .Results hUCMSCs of P3 generation highly expressed the mesenchymal cell marker CD29 and CD105 ,while hardly expressed endothelial cells of CD34 and hematopoietic cell markers .The type Ⅱ collagen composite glycosami‐noglycan scaffold presented white porous foam like ,the porosity was (91 .8 ± 2 .17)% ,the average pore diameter was 110‐230 μm , which was homogeneously distributed and had interpenetration .The scaffold showed good hydrophilicity with the water absorption expansion rate of (213 .71 ± 1 .31)% .The scaffold staining of toluidine blue ,safranin O and type Ⅱ collagen was positive .The car‐tilage‐like tissues were observed ,and gradually increased in the surface of cell‐scaffold complex along with culture ,which were posi‐tive in Toluidine blue ,safranin O and type Ⅱ collagen staining ,the electronic microscopic observation displayed that the cells were actively proliferated in the scaffold ,closely adhered with the materials ,the cartilage‐like cells and a large number of peripheral colla‐gen fibers with zigzag connection could be seen .Conclusion Compositing hUCMSCs and type Ⅱ collagen composite glycosamin‐oglycan scaffold could construct tissue‐engineering cartilage in vitro without induction ,which lays a certain experimental foundation for the repair of cartilage damage .

Objective To analyze the urine trace albumin(mALb)/creatinine(Cr) ratio and blood uric acid(UA),and other various metabolic index level in patients with diabetic nephropathy(DN),combined with clinical data such as patients' age,body mass index(BMI),course of diseases,to explore the related mechanism of occurrence and development of DN.Methods 76 DN patients were selected.The microalbuminuria group(urinary mALb/Cr<300μg/mg) had 46 cases,the clinical albuminuria group(urinary mALb/Cr≥300μg/mg) included 30 cases,another 49 diabetic patients without kidney damage were seleted as control group.The urinary mALb/Cr,blood UA,fasting blood glucose(FBG),triacylglycerol(TG),total cholesterol(TC),high-density lipoprotein(HDL),low density lipoprotein(LDL),glycosylated hemoglobin(HbA1c) levels were determined.The BMI and the length of the course of the disease calculate.Results The patients' age,course of the disease,urinary mALb/Cr,blood UA,FBG,TC,TG,LDL,HbA1c and BMI level in the clinical albuminuria group and microalbuminuria group were significantly higher than those in the control group,the differences were statistically significant (F=6.18,12.48,141.43,12.48,8.49,4.98,6.18,3.89,3.17,3.89,all P<0.05).The high uric acid hematic disease rates of the clinical albuminuria group and microalbuminuria group were 26.09% and 26.09%,which were significantly higher than 10.20% of the control group,the differences were statistically significant(x2=4.074,24.833,all P<0.05).Urinary mALb/Cr was positively correlated with age,duration,BMI,UA,TG,TC,LDL,FBG,HbA1c(r=0.120,0.299,0.148,0.340,0.157,0.149,0.103,0.487,0.103).Multiple linear stepwise regression analysis suggested that duration,blood UA,FBG were independent risk factors of urinary mALb/Cr;TG,BMI,urinary mALb/Cr were independent risk factors for blood UA.Conclusion Urinary mALb/Cr and blood UA are the independent risk factors,high uric acid hematic disease may participate in the development process of DN,and diabetes duration,UA,BMI,TG,TC,LDL,FBG,HbA1C associated with increased urinary mALb/Cr excretory DN patients,the effective monitoring can improve the symptoms of DN and quality of life.

The prepared slices of Chinese crude drugs are growing important in recent years, and faced with new developments and opportunities. The author analyzed the importance of formulate national processing procedures of prepared slices of Chinese crude drugs combined actual work, proposed the overall objectives and tasks for the formulation, and emphasized to need to correctly deal with several important factors during the process of formulate "National processing procedures of prepared slices of Chinese crude drugs", unified the national standards of prepared slices, solved the real problems that the prepared slices of Chinese crude drugs industry faced.
Chemistry, Pharmaceutical ; methods ; standards ; China ; Drugs, Chinese Herbal ; chemistry ; Humans ; Medicine, Chinese Traditional ; methods ; standards

Objective:To explore the effect of tension-reducing distraction of external fixators for limb wound closure.Methods:A retrospective case series study was made on data of 21 patients with refractory limb wounds admitted to 920th Hospital of Joint Logistics Support Force of PLA from January 2016 to July 2019. There were 15 males and 6 females，aged 11 to 56 years ［ （37.8 ± 11.2）years］. The lower-limb wounds were found in 15 patients and the upper-limb wound in 6 patients. The area of wound defect ranged from 1.2 cm × 1.0 cm to 22.0 cm × 17.1 cm. After debridement of the limb wound，the tension-reducing distraction of Ilizarov external fixators was used according to the shape and location of the wound. Distraction was applied at a rate of 1 mm/d starting at postoperative day 1 and the direction of distraction was adjusted according to the angle of skin closure of the trauma. The wound reduction distraction time and wound healing time were recorded. The wound healing score was used to evaluate wound healing at postoperative 5 days and 30 days. Complications were recorded according to the Paley classification. The wound survival curve was plotted to observe the time of wound tension reduction and traction and wound healing. The final wound healing was detected as well.Results:All patients were followed up for 3 to 9 months ［（4.5 ± 1.7）months］. The wounds were significantly reduced after reduction and distraction，with the reduction time of 6-23 days ［（7.8 ± 2.3）days］ and the wound healing time of 15-47 days ［（23.0 ± 3.3）days］. The wound healing score was （3.2 ± 0.9）points at postoperative 5 days and （0.7 ± 0.2）points at postoperative 30 days （ P < 0.05）. There were 15 problems，0 disorder，and 0 sequelae according to the Paley classification of complications. The wound survival curve showed the median time of reduction and distraction and wound healing was 16 days and 34 days，respectively. All patients showed wound healing without recurrence，of which 10 were healed directly，8 by implants and 3 by sutures. Conclusion:For patients with limb wounds difficult to be treated by flap coverage，the tension-reducing distraction of external fixators can effectively close the wound，fasten wound healing，shorten treatment period，and has fewer complications and a low recurrence rate.