Objective To study a method for the determination of heterophyllin B inTaizishen- Huangqi oral solution.Methods The HPLC system consisted of Phenomenex Luna-C18 (4.6 mm × 250 mm, 5μm) column, the mobile phase consisted of acetonitrile and 0.1% acetic acid, the gradient elution flow rate was 1.0 ml/min, the column temperature was 30℃, and the UV detector was set at 203 nm.Results The linear response range was 0.016-0.320 mg/ml (r=0.999 8). The average recovery of heterophyllin B was 98.92%, and RSD was 0.80%. The average determination of Heterophyllin B within 5 batches of preparation was 1.63 mg/ml. Conclusions The method showed its high sensitivity, accurate, repeatable and high secificity. It could be used as a method to control the standard ofTaizishen-Huangqi oral solution.

Objective To evaluate the effect of nursing interventions on the quality of life of patients undergoing continuous ambulatory peritoneal dialysis (CAPD).Methods Randomized controlled trials (RCTs) involving nursing interventions were collected from the databases of Cochrane,PubMed,Elsevier Science Direct,VIP,CNKI and WanFang.Data were analyzed with RevMan 5.1 software.Results 8 articles met the inclusion criteria.The results of Meta-analysis showed that there was considerable heterogeneity across the analysis,which might be resulted from length of intervention and patients of different ages according to subgroup analysis,and that gender was not the factor causing heterogeneity.In the experimental group,both physical and emotional aspects after receiving nursing interventions were significantly improved than the control group.Conclusions Nursing interventions can improve the quality of life of patients with CAPD.

Objective: To investigate the peripheral blood mucosa-associated constant T cell(MAIT) expression in children with bronchial asthma and its relevance to severity. Methods: Ninety-eight children with bronchial asthma who were treated in Affliated Dongfeng Hospital from May 2016 to May 2019 were selected as the asthma group. Another 60 healthy children who underwent health checkup during the same period were as control group. The levels of MAIT were detected by flow cytometry analyzer using BD FACSAria II flow cytometry, and the lung function was detected by Yage lung function tester. The receiver operating characteristic (ROC) curve was used to evaluate the diagnostic efficacy of peripheral blood MAIT for bronchial asthma. Pearson analysis was used to examine the relationship between MAIT cells and pulmonary function. Results: There were no significant differences in the general data of gender, age, and body mass index between the two groups (P>0.05). The asthma control test (ACT) score, the percentage of first second forced expiratory volume occupied estimated value (FEV₁%), the percentage of first second forced expiratory volume occupied forced vital capacity (FEV₁/FVC), the percentage of peak expiratory flowoccupied estimated value (PEF), and MAIT cells in asthma group were significantly lower than those in control group (P<0.05). Serum MAIT identified bronchial asthma and healthy controls with an area under curve (AUC) of 0.900, with a sensitivity of 82.65%, and a specificity of 85.00%. Serum MAIT identified the mild-sustained group and the healthy control group with an AUC of 0.910, with a sensitivity of 94.64%, and a specificity of 81.67%. Correlation analysis showed that MAIT cells in children with bronchial asthma were significantly positively correlated with pulmonary function indexes such as FEV₁%, FEV₁/FVC and PEF (r=0.159, 0.222, 0.213, P<0.05). Conclusions The peripheral blood MAIT in children with bronchial asthma is significantly reduced, which is related to the levels of lung function indicators. MAIT can be used as one of the indicators for the diagnosis and clinical prognosis of patients with bronchial asthma.

Objective To study the effects of Schwann cell-derived neurotrophic factor(SDNF) on myoblast stem cells(called satellite cells,SCs) in vitro. Methods After setting up SCs culture system in vitro, SCs which treated with various SDNF concentrations culture medium were dynamically evaluated by cell morphology,MTT growth curve and fusion rate. Results The ability of SCs preceding their participation in muscle repair include proliferation and differentiation, 200 ng/ml SDNF stimulated cell proliferation more than the other medium,but 50 ng/ml,100 ng/ml,200 ng/ml,400 ng/ml SDNF made SCs differentiation significantly for their high myotube fusion rate. Conclusion SDNF can regulate the proliferation and differentiation of rat skeletal satellite cellsin vitro,but in differentiation significantly.SDNF might play a role in slowing down denervated muscle atrophy.