Objective To determine the most appropriate method in the treatment of choledocholithiasis. Methods The clinical data of 135 patients who received either laparoscopic common bile duct exploration (LCBDE) or laparoscopic transcystic common bile duct exploration (TC-BDE) from May 2005 to July 2009 were retrospectively studied. Results All patients were cured of choledocholithiasis. In 13 patients three trocars were used, in 89 patients four trocars were used, and in 33 patients conversion to open operation was done. Of the 102 patients in whom minimal invasive surgery was successfully carried out, TC-BDE was done in 6 patients and LCBDE in 96 patients. Primary closure of common bile duct (CBD) was carried out in 33 patients while T-tube drainage was done in 63 patients. Six patients had a transient bile leak which healed spontaneously in 3 to 4 days. Retained stone was detected in 2 patients which was successfully retrieved through choledochoscopy. Conclusion An individualized treatment approach is the most reasonable way to manage CBD stones. The choice of the procedure depends on the number and size of stones, the surgeons experience and the patient's choice.

Relative problems in the management of HIV antibody primary screening lab are discussed, including laborato-ry building, staffing and training, equipment management, document management, reagent management, quality control and security protection. It is pointed out that the standardized laboratory management is an important part of the HIV antibody primary screening lab's quality Control.

Snake venoms are complex mixtures containing many different biologically active applications in the field of cancer research. In this paper, not only unpurified fraction, but also highly purified snake venoms are discussed,such as rhodostomin, acutobin, agkistrodon halys antithrombotic enzyme, batroxobin. The review mainly introduced the inhibitory effects of snake venoms on tumer metastasis.

0.05). Follow-up of 14 patients was from 1 to 60 months (the mean value is 26. 8 months). There was no late death and re-operation. The average mitral annulus area is (3.00?0.57) cm~2. The transmitral pressure gradient decreased from (8.13?4.49) mmHg to (6.25?3.82) mmHg after operation (P=0.050). There were eight cases with minimal reflux and 6 cases with slight reflux. In all patients, the heart function returned to NYHA I～II grade. Conclusion The “edge-to-edge” technique is a feasible and effective operation for treatment of the mitral regurgitation caused by Barlow's disease.

Objective To explore the role of endothelial progenitor cells (EPCs)in improving functional recovery and promoting neurogenesis in damaged area of ischemic rat brain by MRI,neurological severity score (NSS),and pathological examinations.Meth-ods EPCs wereseparated,cultured,and identified,ultrasmall superparamagnetic iron oxide(USPIO)particles was used to label EPCs in vitro.Transient middle cerebral arterial occlusion(tMCAO)was successfully performed in 30 adult SD rats.Magnetically labeled cells(the experimental group)and normal saline (the control group)were injected intravenously into the tMCAO rats through the tail vein respectively,then MR imaging and NSS was performed 0 day,2 days,4 days,6 days and 8 days later.At last the paraffin-embedded rat brain tissues were obtained and examed by Prussian blue staining and Measurement of microvessel density.Re-sults EPCs wereseparated,cultured,and identified successfully.MR imaging showed significant low signal intensity at the ischemic area on T2 WI sequence.Prussian blue staining images were corresponding to the EPCs staining in vitro.The NSS and microvessel density in experimental group were significantly higher than in control group.Conclusion EPCs can migrate to the damage zone, improve functional recovery and promote neurogenesis in damaged area of ischemic rat brain,and may be a new source of multipoten-tial stem cells for stroke treatment.

OBJECTIVE To avoid the hospital(infection) in the dental surgery operating room.METHODS The(international) ISO quality standard was applied to establish the quality control the hospital infection.RESULTS The quality control for the hospital infection was guaranteed in the dental surgery operating room,and the items (observed) were in the allowance of the legislations for the hospital infection.Zero case of hospital infection(happened.) CONCLUSIONS It is useful for the hospital infection administration in the dental surgery operating room to apply the international ISO quality standard.

Objective: To compare postoperative recovery situation between laparoscopic radical resection and conventional open surgery for patients with colorectal cancer and determine the optimal surgery scheme. Methods: 96 patients with colorectal cancer were divided into observation group (48 cases)and control group (48 cases) according to the random number table method. The patients of observation group were treated by using laparoscopic radical resection while the patients of control group were treated by conventional open surgery. A series of clinical manifestation, such as perioperative situation, abdominal pain, recovery situation, complication, pre-and post operative nutrition and quality of life, of these patients were compared between the two groups. Results: The operation time, average bleeding volume, postoperative exhaust time and hospitalization time of the observation group were significantly less than those of the control group (t=5.583, t=48.934, t=7.347, t=4.862; P<0.05). The incidence of abdominal complication of observation group (8.3%) was significantly lower than that of the control group (27.1%) (x2=5.79, P<0.05). The incidence of delayed healing of incision was 6.3% in observation group, which was significantly lower than that (20.8%) of control group (x2=4.36, P<0.05). For observation group or control group, the serum ALB, PA and LC of post-operation, respectively, was higher than those of pre-operation in 1 week after operation. And serum ALB, PA and LC of observation group, respectively, was significantly higher than that of control group (t=4.628, t=3.642, t=3.412; P<0.05). Compared with before operation, physical function, role function, emotional function, cognitive function and social functionthe quality of life of the two group were obviously improved, on the other hand, all of these aspects of the observation group were significantly higher than those of the control group (t=-12.845, t=-7.634, t=0.5001, t=-4.096, t=-2.647, P<0.05). Conclusion: Compared with the traditional open surgery for patients with colorectal cancer, the postoperative recovery of laparoscopic radical resection is better, more obvious and more effective for them.

OBJECTIVE:To develop a method for simultaneous determination of chlorogenic acid,baicalin,aloe emodin,rhe-in,emodin,chrysophanol and physcione in Kangjun xiaoyan tablets. METHODS:RP-HPLC method was adopted. The determina-tion was performed on Agilent Extend C18 with mobile phase consisted of acetonitrile-0.5% phosohoric acid solution(gradient elu-tion)at the flow rate of 1.0 mL/min. The detection wavelengths were set at 327 nm(chlorogenic acid),280 nm(baicalin)and 450 nm(aloe emodin,rhein,emodin,chrysophanol,physcione). The column temperature was 30℃,and sample size was 10μL. RE-SULTS:The linear ranges of chlorogenic acid,baicalin,aloe emodin,rhein,emodin,chrysophanol and physcione were 0.2094-4.1880μg(r=0.9999),0.3720-7.4400 μg(r=0.9999),0.0063-0.1262 μg(r=0.9999),0.0116-0.2322 μg(r=0.9999),0.0053-0.1060 μg(r=0.9998),0.0128-0.2564 μg(r=0.9999),0.0165-0.3303 μg(r=0.9998),respectively. The limits of quantita-tion were 2.01,1.93,0.76,1.25,1.24,0.53 and 1.53 ng;the limits of detection were 0.98,0.65,0.25,0.42,0.41,0.18 and 0.52 ng,respectively. The recoveries were 98.41%-100.40%(RSD=0.76%,n=9),96.17%-100.10%(RSD=1.58%,n=9), 96.11%-100.10%(RSD=1.33%,n=9),96.29%-100.80%(RSD=1.85%,n=9),96.88%-100.10%(RSD=1.22%,n=9), 97.81%-101.90%(RSD=1.64%,n=9),96.46%-101.30%(RSD=1.85%,n=9),respectively. CONCLUSIONS:The method is accurate,reliable and reproducible. It can be used for simultaneous determination of 7 components in Kangjun xiaoyan tablets.

Problem-based learning (PBL) teaching method can improve students' ability of study,analysis and problem-solving.Network platform based PBL teaching mode combines the network education and PBL teaching mode; it has clear superiority in information acquisition,communication and transmission.Furthermore,it can also solve the problem of inadequate teaching sources.Network platform based PBL teaching mode was applied in neurology teaching to investigate the best scheme and form for teaching plan compilation,network platform building-up and teaching process implementation.At the same time,teaching effect was evaluated and summarized in an aim to improving neurology teaching quality and speeding up the reform of network-based PBL teaching.

Objective To assess the therapeutic effect of imaging-guided pulsed-radiofrequency stimulation of medial branch of lumbar spinal nerve in treating lumbar facet joint syndrome. Methods A total of 48 patients with clinically-diagnosed lumbar facet syndrome were randomly divided into group A (anti-inflammatory and analgesic group, n=26) and group B (pulsed-radiofrequency group, n=22). The patients of group A received oral meloxicam dispersible tablets , while the patients of group B were treated with DSA-guided pulsed-radiofrequency stimulation of dorsal medial branch of lumbar spinal nerve corresponding to the diseased lumbar facet. Using visual analogue scale (VSA) the severity of pain at the time of admission, discharge and 6 month after discharge was separately evaluated , and the therapeutic effect was assessed based on the improvement of VSA score. Results The VAS scores of group A and group B at the time of discharge and 6 months after discharge were significantly lower than those determined at the time of admission (P<0.01). At the time of discharge, the VSA score of group B was significantly lower than that of group A (P<0.05). Six months after discharge, the VAS score of group B was significantly lower than that of group A (P<0.05). The total effective rates at 6 months after di scharge of group A and group B were 46.5% and 87.6% respectively;the excellent effect rates of group A and group B were 26.4% and 76.2% respectively. Both the total effective rate and excellent effect rate of group B were significantly higher than those of group A (P<0.01). Conclusion Imaging-guided pulsed-radiofrequency stimulation of dorsal medial branch of lumbar spinal nerve can effectively relieve chronic lower back pain caused by lumbar facet joint syndrome , and its therapeutic effect is superior to oral anti-inflammatory and analgesic medication.