Objective: To explore the compatibility and application methods of clinical formulas.Methods: In the guidance of monarch,minister,assistant and guide theory, the constructions of prescription were divided into principal agents and lesser agents;500 prescriptions were from 1000 set prescriptions and empirical formulas recorded in pharmacology of traditional Chinese medical formulae teaching material and well-chosen efficacy nostrum written by national lever famous veteran teran doctors of TCM.Then they were analyzed to study the constituting formats of(clinical) formulas and the derivation methods of set prescriptions in clinical application.Results: Through analysis,we found the constituting formats of formulas in terms of single taste single group having principal agents but lesser agents(12.3%),multitude groups having principal agents but lesser agents(13.7%),single taste or single group principal agents with one group lesser agents(14.4%),multitude groups principal agents with one group lesser agents(17.7%),single taste or single group principal agents with multitude groups lesser agents(18.3%) and multitude groups principal agents with multitude groups lesser agents(23.6%) et al.From the 500 randomly extracted prescriptions,among 68.4% prescriptions were present derivation relationship,that is,for adapting new pathogenesis conditions,through methods in terms of replacing principal agents(10.2%),mild recipe lowering to lesser agents(17.0%),two formulas to put together(14.3%),expanding principal agents(16.4%),changing lesser agents(18.7%),principal agents pero-replacing(10.2%) and others(13.2%),changed constituted relation of principal agents or lesser agents,so to generate several formulas possessing new effect characteristics.Conclusion: The(divide) of principal agents and lesser agents has a universal applicability in analyzing the constitutions of formulas;the clinical application and derivation methods of set prescriptions revealed by this method have guide effect to initial(experiencing) clinical worker.

Adolescent ; Humans ; Laryngeal Neoplasms ; Male

Objective To investigate the effects of Astragalus polysaccharide (APS) on damage of keratinocytes in ultraviolet B (UVB) radiation skin in HaCaT cells; To preliminarily explore its mechanism of action.Methods HaCaT cells were treated by UVB irradiated for modeling. Blank control group, model group, UVB irradiation group and low-, medium- and high-dose APS interventional groups were set up. Each medication group was given relevant medicine. MTT assay was used to determine the cell activity; GSH-Px, CAT, SOD activity and MDA content were detected by biochemical colorimetric method; contents of TNF-α and IL-6 were detected by ELISA.Results Compared with the control group, the contents of SOD, CAT and GSH-Px in the model group decreased significantly, while the contents of MDA, TNF-α and IL-6 increased significantly (P<0.05); compared with the mode group, the contents of SOD, CAT and GSH-Px in the medication groups increased significantly, while the contents of MDA, TNF-α and IL-6 were reduced significantly (P<0.01).Conclusion APS can reduce the oxidative stress injury of UVB to HaCaT cells to some extent.

Objective:To explore effects of Baoyuan Huoxue Granule on senile type 2 diabetes with Qi deficiency and blood stasis,and expression rates of membrane glucoprotein selectin P(CD62p)and fibrin receptor(PAC-1)in the granule of platelet.Methods:60 cases of senile type 2 diabetes with Qi-deficiency and blood stasis were randomly divided into two groups,a simple western medicine control group(n=30)and a test group(n=30)treated by routine western medicine and Baoyuan Huoxue Granule.The treatment was given 2 months.Their clinical therapeutic effects,and the expression rates of CD62p and PAC-1 before and after treatment were investigated.Results:In the test group,12 cases(40%)were markedly effective,15 cases(50%)effective,3 cases (10%)ineffective,with an effective rate of 90%;In the control group,3 cases(10%)were markedly effective,12(40%) effective,15(50%)ineffective.the effective rate being 50%,with significant difference between the two groups in total effective rate(P

Objective To explore the mechanism of therapeutic effect of Yichantang in the treatment of Parkinson′s disease (PD). Methods A rat model of PD was replicated. The experimental animals were divided into a normal control group,a normal saline control group and a Yichantang group. Changes in the rats' behavior pattern was obrerved. Using the technique of intracerebral dialysis,the content of catecholamine like substances in the nigral extracellular fluid(NECF)was measured by means of high performance liquid chromatography(HPLC). Changes in the affinity and quantity of M and DA receptors were determined too. Results Yichantang markedly improved the rats' rotation behavior,and enhanced the Bmax and KD of DA and M receptors in the brain tissues on the side of lesion. The levels of DOPAC,HVA,DA and 5-HT in the NECF of the side of lesion in the Yichantang group were significantly higher than that of the normal saline group ( P

280r/40min). So the PD model was regarded eligible. In the site of lesion in SN, the number and volume of dobaminergic neurons were decreased, and the tissue structure of SN became indistinct, the levels of dopamine (DA), dihydroxyphenylaceticacid (DOPAC), homovanillicacid (HVA) 5-hydroxytryptamine (5-HT) and 5-hydroxyindoleaceticacid (5-HIAA) were more conspicuously reduced than that in uninjured side. TH-immunoreactive cells of substantia nigra compact part were lessened obviously on injured side. The abnormal rotatory behavior of PD rats did not spontaneously disappear during 10 months of continuous observation. Conclusion The PD rat model, which is reproduced by injecting 6-OHDA to selectively destroy dopaminergic neurons in the substantia nigra of rat, is stable and reliable, and demonstrated similar primary pathological changes of PD.

BACKGROUND: Parkinson disease (PD) not only influences motor function, also affects sensation and cognition. Treatment with dopamine can only control symptom and has well side effect; however, yichan tang has great effect on treating PD in clinic.OBJECTIVE: To observe the effect of yichan tang on praxiological behavior, catecholamines substance in cerebral tissue and substantia nigra nerve cells of PD rats and analyze its effective mechanism.DESIGN: Randomized control animal study and single-blind evaluation.SETTING: Department of Traditional Chinese Medicine of General Hospital of Chinese PLA.MATERIALS: A total of 120 female SD rats weighing 180-200 g were selected in this study. Seventy modeling PD rats were randomly divided into saline group (n=30) and yichan tang group (n=40) according to digital table. Another 30 non-modeling rats were regarded as normal control group. Yichan tang was made of 15 g shanyurou, 15 g shichangpu, 10 g xianlingpi, 10 g roucongrong, 15 g gouqizi, 15 g danshen and 8 g wugong,etc., and provided by Dispensary of Chinese herb of General Hospital of Chinese PLA. Drug was decocted for 30 minutes to filter the solution and then concentrated into decoction including 1.0 g/mL raw materials.METHODS: The experiment was carried out at the Animal Laboratory of General Hospital of Chinese PLA from September to December 2001. PD rat model were established with 6-hydroxy-dopa to observe behavior of rats.The administration of rats (body mass/kg) in yichan tang group was 10 times as the administration of the adults (60 kg) (5 g/kg). Rats were perfused once a day and weighted once a week. The dosage of administration was regulated as the body mass changing, and the administration lasted for 8 weeks. Rats in saline group and normal control group were perfused with the same volume of saline as the criteria of body mass. Rotatory behaviors of rats in normal group, saline group (model group before administration)and yichan tang group were observed before administration and at 5, 6, 7 and 8 weeks after administration. Changes of content of catecholamines in extracellular fluid of cerebral tissue were measured with micro-dialysis technique and high performance liquid chromatography (HPLC), meanwhile, changes of substantia nigra nerve cells on both sides of corpus striatum were observed under 20-time and 40-time optic microscopes.MAIN OUTCOME MEASURES: Effect of yichan tang on medium of cranial nerve of PD rats; changes of substantia nigra nerve cells on both sides of corpus striatum with optic microscope and electron microscope; evaluation of yichan tang.RESULTS: Among 100 rats, 6 rats in yichan tang group died because of failure perfusion, 5 in saline group and 2 in normal control group. Therefore, 87 rats were involved in the final analysis. ① At 5, 6, 7 and 8 weeks after treatment, rotatory times within 40 minutes were all shorter in yichan tang group than those in saline group [(112.34±33.36), (91.16±73.06),(72.05±20.77), (61.63±17.93), (340.90±52.97) times, P ＜ 0.01]. ② Levels of 3,4-dihydroxybenzene acid, homovanilic acid, dopamine and 5-serotonin on the injured side were lower than those on the uninjured side (P ＜ 0.05-0.01). After treatment, levels of mediums mentioned above were higher in yichan tang group than those in saline group (P ＜ 0.05-0.01); however,there were no significant differences of mediums on the uninjured side in the three groups (P ＞ 0.05). ③ Pathological observation: Amount of substantia nigra nerve cells on the injured side was more in yichan tang group than that in saline group; moreover, volume of neurons was full, structure was clear, and values of Golgi complex and mitochondria were close to the normals.CONCLUSION: Yichan tang can relieve injured degrees of substantia nigra nerve cells, facilitate the repair, increase the content of endogenetic catecholamines in cerebral tissue, and improve rotatory behavior of PD rats.

Objective: To investigate the effects of traditional Chinese medicine Baoyuanhuoxue Granula on the state of senile type Ⅱdiabetes before thrombus.Methods: 60 cases of selected senile type Ⅱdiabetes patients were divided into two groups,trial group(treatment with routine western medicine added Baoyuanhuoxue Granula;30 cases) and control group(treatment with simple western medicine; 30 cases).After two months,the changes of expression rate of blood viscosity, nail fold microcirculation and platelet aggregation,D-D,CD-62p and PAC-1 of patients in two groups before and after treatment were observed.Results: Baoyuanhuoxue Granula can reduce the expression rate of blood viscosity,platelet aggregation,D-D,CD-62p and PAC-1 obviously,and can improve nail fold microcirculation.Conclusion: Baoyuanhuoxue Granula can improve the state of senile type Ⅱdiabetes before thrombus.

Objective:To study and design measurement scale which can judge and survey sub-health state and itscharacteristic of TCM syndrome.Methods:Making use of methods of scale technique,literature research,expertise consultation et al,based on TCM theory,via conceptualization and operation of sub-health and TCM syndrome,forming structure and type of scale,establishing item pool and results,and then through item selection,assessment of validity and reliability of pre-investigation,making the measurement scale.Results:The scale is made up of 4 parts:body function state,psychological function state,social function state and particular state of TCM.The 4 parts are divided into 15 sorts,including 120 items.Conclusion:The scale contributes much to the diagnosis of sub-health state and the clari cation of clinical manifestations,features,distributions of TCM syndrome of public of sub-health,which is one of the most important methods for investigating TCM syndrome of sub-health state.

AIM: To approach the antiobesity action and mechanisms of the daidzein derivative: LRXH609(LRX).METHODS: The body weight,Lee′s index,total weight of celiac fat tissue,food intake,serum glucose and lipids in obese rats induced by a high-fat diet were measured and the antiobesity action was tested after LRX was administered for 30 days.3T3-L1 pre-adipocytes were induced by in vitro culture,the effects of LRX on cell proliferation,lipogenesis,lipolysis were observed.RESULTS: The body weight,Lee′s index,fat tissue weight in obese rats were significantly decreased by LRX,and the concentrations of TC,FFA in serum were decreased,the proliferation of 3T3-L1 pre-adipocytes was inhibited,the activities of hormone sensitive lipase in 3T3-L1 pre-adipocytes were significantly elevated,the glycerine release from adipocytes was promoted and the concentrations of TG in adipocytes were decreased.CONCLUSION: LRX plays a role in antiobesity action and regulating blood lipid and the mechanism might be related to inhibiting proliferation and differentiation of pre-adipocytes,stimulating TG decomposition by activating hormone-sensitive lipase and decreasing the TG storage in adipocyte.