Cyclosporine A (CsA)has been widely used in steroid-dependent and steroid-resistant nephrotic syndrome as a common drug.However,renal toxicity can often occur at the same time which limited the use of CsA.CsA can lead to acute and chronic renal damage,ischemic renal tissue inflammation,and fibrosis.Currently in clinical practice,urine micro proteins and serum creatinine have been used to monitor CsA associated nephrotoxicity.However,this method cannot detect CsA associated renal damage in early stage.At present,a hot topic about CsA focuses on looking for a better non-invasive and sensitive way to monitoring CsA associated nephrotoxicity.This article reviews the current progress of known acute kidney injury biomarkers in momtoring CsA associated nephrotoxicity.

Object To research the percutaneous absorption of ?-asarone in various solvents in vitro. Methods To measure the concentration of ?-asarone passed through rat skin in various solvents which contained borneol and azote-ketone by HPLC, count the penetration of accumulation within 24 h and steady velocity. Results 0.1% Borneol could not promote the penetration of ?-asarone; azote-ketone could decrease the penetration of ?-asarone. The penetration of accumulation within 24 h and steady velocity of ?-asarone in 20% ethanol were (352.624?87.049) ?g/cm2, (18.902?4.840) ?g/(cm2?h), respectively. Conclusion The percutaneous absorption of ?-asarone is the best when the solvents contain no promoter.

Objective To study the protective effects of ?-asarone on PC12 cells damage induced by Glutamate. Method The effects of ?-asarone on PC12 cells after Glutamate intoxication on morphology, extent of damage, livability, intracellular calcium concentration and apoptosis ratio were observed. Result Morphological changes, LDH leakage and intracellular calcium concentration increasing, and cell survival decreasing were observed in PC12 cells exposured to Glutamate. 7.5, 15, 30 ?g/mL?-asarone can increase cell survival, decrease LDH leakage. 15, 30 ?g/mL ?-asarone can reduce intracellular calcium concentration and apoptosis ratio. Conclusion ?-asarone prevents the toxicity of Glutamate, and it maybe attribute to its effect of anticalcium.

Objective:To study the alteration of K-ras mutations in different stages of colorectal cancer(CRC) and its influence on the progression of CRC. Methods:The 20 paraffin-embedded tissues, including primary foci, metastatic lymph nodes, remoter metastatic foci, colorectal adenoma, and normal colorectal tissues, were collected from 20 patients with colorectal cancer. The sequence of PCR-amplified products were analyzed. Results:The wild K-ras gene was expressed in normal colorectal tissues. The mutation frequency of K-ras gene was 20.0% (4/20) in colorectal adenoma, 30.0% (6/20) in primary foci, 25.0% (5/20) in metastatic lymph nodes, and 30% (6/20) in remote metastatic lesions. In the samples with K-ras mutations, the consistency of the types of K-ras mutations between primary foci and colorectal carcinoma, lymph node metastatic lesions, remote metastatic lesions was 0.0%(0/4), 40.0%(2/5), and 50.0%(3/6), respectively.Conclusion:The colorectal adenoma, metastatic lymph nodes and remote metastatic lesions were not suited for K-ras analysis as routine samples in clinical practice. If the samples of primary lesions were not available, the detection results of metastatic lymph nodes and remote metastatic remote lesions will provide some reference values. K-ras gene had several different mutations in the progression of CRC.

A girl who aged eight years and seven months presented with prunosus patches on the right buttock for 8 years,gradual unilateral enlargement of the right lower limb for more than 7 years,and multiple vegetations for 1 year.Dermatological examination showed nevus flammeus and multiple malodorous vegetations over the right lower limb with high skin temperature.The right lower limb was thicker and longer than the left lower limb.X-ray examination,magnetic resonance imaging and Doppler ultrasound examination revealed high-flow vascular malformations.Pathological examination of the vegetations showed vascular proliferation,fibroblast proliferation and erythrocyte extravasation.She was diagnosed as Parkes-Weber syndrome accompanied by pseudo-Kaposi's sarcoma.

OBJECTIVE:To evaluate the stability of 12 kinds of submicro emulsion in market,and screen the test method for the stability. METHODS:12 kinds of submicro emulsion in market were selected,high pressure sterilization (121 ℃,30 min), high speed centrifugation(4000 r/min,15 min),accelerated test [placing 6 months under temperature of(40±2)℃,relative hu-midity of (75 ± 5)%] were conducted to investigate the pH,particle size and other indexes,and SPSS 22.0 was used to analyze the distribution variance and chi-square test,and investigate the correlation of 3 evaluation methods. RESULTS:In terms of stabili-ty investigation,the pH value of 12 kinds of submicro emulsion decreased to some extent after accelerated test,average particle size of 6 kinds of submicro emulsion samples were greater than 300 nm,the variance of the particle size distribution of 9 kinds ap-peared in 0.05-0.15,the chi-square test results of 8 kinds distributed below 1. The average particle size of 4 kinds of submicro emul-sions changed more than 10 nm after accelerated test. In terms of stability test method,Pearson chi-square progressive significance of high pressure sterilization and accelerated test was 0.665,which was higher than 0.05,indicating there was no correlation (no significance),the stability results of high pressure sterilization can not represent the results of accelerated test;that of high speed centrifugation and accelerated test was 0.004,which was lower than 0.05,indicating stability results between high speed centrifuga-tion and accelerated test results were significantly correlated. CONCLUSIONS:The submicro emulsion in market can meet the re-quirements of stability. To a certain extent,high speed centrifugation can replace the acceleration test.

The main clinical symptoms of Parkinson's disease (PD) are resting tremor, muscle rigidity, bradykinesia, and so on. There is no effective treatment for PD yet, and dyskinesia symptoms affect the life qualities of PD patients. The therapy used for reinforcing kidney and activating blood circulation in treatment of PD can achieve good clinical effects.

AIM: To study the therapeutic effects and the related mechanism of Pingchuanling(PCL) on bronchial asthma in rats. METHODS: The rats asthma model was established by ovalbumin(OVA) sensitization.The SD rats were divided into the normal control group,asthma model group,dexamethasone group,low and higher dose of PCL group.Eos in serum and BALF were taken count of and tissue slice dyed with HE were observed.In addition Fas,Bcl-2 expression in lung tissue were examined by immunotisssuchemical technology. RESULTS: Eos count in serum and BALF of asthma model group were increased significantly as compared with that in normal animals.Asthma induced delitescence also were shorten obviously,the differences between the groups were all significant(P

Objective To explore the value of superb microvacular imaging ( SMI) technology in differentiating thyroid microcarcinomas from benign micronodules comparing with contrast‐enhanced ultrasound (CEUS) . Methods A total of 195 thyroid micronodules ( ≤1 .0 cm) from 172 patients were examined using CEUS and SMI ,and the findings were reviewed and evaluated in comparison to the pathological results . Results Of all the 195 thyroid nodules ,pathologic findings revealed that 140 (71 .8% ) were malignant and 55 (28 .2% ) were benign ,CEUS diagnosed that 127 (65 .1% ) were malignant and 68 (34 .9% ) were benign ,and SMI diagnosed that 129 (66 .2% ) were malignant and 66 (33 .8% ) were benign . There were no differences between the SMI and CEUS in terms of sensitivity ( 90 .0% vs 87 .9% ) , specificity ( 94 .5% vs 92 .7% ) ,accuracy ( 91 .3% vs 89 .2% ) ,positive predictive value ( 97 .7% vs 96 .9% ) and negative predictive value ( 92 .9% vs 87 .9% ) ( P > 0 .05 for all) . Conclusions SMI can effectively observe the perforating blood vessels of thyroid micronodules , which plays an important role in differentiating of benign and malignant thyroid micronodules .

In the guidance of system theory, using Multidisciplinary technology to study on the combination of Chinese traditional and western medicine, helps to promote fingding the common points between the Chinese traditional and western medicine. In this article, it intends to create mathematical model and analysis of correlation between Chinese medicinal characteristics and neurobehavioral effects based on literature informatics. The numbers of the Chinese medicines with neurobehavioral effects were worked out within the frame work of 'The China Pharmacopeia' of 2005 edition, from the literature publicized since 1980. The correlation and mathematical model were figured outbetween Chinese medicinal characteristics including traditional functional classification, different tastes as well as channel tropism and immunoregulatory activity based on the statistical software SPSS. The results showed that the neurobehavioral effects was related to the qi-invigorating drugs, yang-invigorating herbs, blood-invigorating herbs, resuscitative drugs and kidney meridian herbs, tastes mild of Chinese medicines (P<0.05). It also accords with the theory of 'Kidney produce marrow' of TCM, The coincidencewas 82.8% between mathematical computing and original classification. There are correlations between Chinese medicinal characteristics and neurobehavioral effects. The mathematical model based on these results can be used for studing of neurobehavioral effects and clinical treatment.
China ; Clinical Laboratory Techniques ; Diagnosis, Differential ; Drugs, Chinese Herbal ; pharmacology ; Humans ; Medicine, Chinese Traditional ; methods ; trends ; Models, Theoretical ; Nervous System Diseases ; Statistics as Topic