Forty-six male SD rats were randomly divided into 3 groups:control group(Grp 1), exhaustive swimming group(Grp 2) and recovery from swimminggroup (Grp 3). The lipid peroxidase (LPO) content and superoxide dismutase(SOD) activity of kidney, the excretion rate of total urinary protein (TUP)and the plasma angiotensin II(AII) content were measured. Their ultrastructuralchanges of kidney were investigated. It was shown that after exhaustive swimming,the LPO contents of kidney,the AII and the excretion rate of TUP increased significantly (p0.05). In Grp 3,the LPO and theexcretion rate of TP were significantly higher than Grp 2 (p

Objective To investigate the effects and safety of human hepatocytes transplantation in vivo for the treatment of liver failure. Methods The primary human hepatocytes were collected from normal liver tissue donated by healthy volunteers and preserved by cryopreservation technique. After thawing, the hepatocytes were transplanted into the spleen of patients with severe hepatitis through catheterization of the femoral artery. Then the changes in clinical symptoms, serum biochemical indexes and MRI signals of the spleen were observed in the patients. Results A total of 2?10 10 hepatocytes were isolated from normal liver tissue of healthy volunteers and 75% of the hepatocytes were alive after cryopreservation and thawing. The number of transplanted hepatocytes was 2?109. In the recipients, the clinical symptoms were markedly improved, serum levels of bilirubin, NH_3, ALT and AST were significantly reduced, but that of PTA remarkably increased, after hepatocyte transplantation. The follow-up examination was performed 80d and 270d after discharge from the hospital, and it was showed that all the serum biochemical indexes returned to normal and signals of the hepatocytes were found in the spleen. Conclusions Hepatocyte transplantation is a safe and effective therapy for severe hepatitis. The transplanted hepatocytes can proliferate and differentiate in the spleen to replace or partially compensate the liver function of synthesis, detoxication and metabolism. Contrast enhanced MRI can be a new method for follow-up study of transplanted hepatocytes.

Kidney deficiency is the core of the currence of multiple sclerosis.Invigorating the kidney is the core in treatment of multiple sclerosis,though there are some other commonly used methods,such as dissipating phlegm,promoting blood flow,clearing heat,dispelling dampness and diuresis in aspects of eliminating pathogen,and invigorating spleen for benefiting qi,dispersing stagnated liver-qi,calming mind for tranquillizing in aspects of regulating the function of organs.Either invigorating the kidney is used through the whole course of disease or used at the later stage,it plays an important role in treatment of multiple sclerosis.

Objective To investigate the effect of Mannatide capsules and Thymic peptide on children with recurrent respiratory tract infections(RRI) and the influnence on their immunity. Methods 160 patients with recurrent respiratory tract infections were randomly divided into the control group and the treatment group,the treatment group were treated with Mannatide capsales and Thymic peptide while the control group with only the routine therapy. Results The effect of the treatment group was over the control one(P＜0.01),two groups of the patients were promoted on Ig level,and the effect of 160 cases in the treatment group was more obvious than that of the control one(P＜0.05). Conclusion Mannatide and Thyrnic peptide applied together are effective in treating children with recurrent respiratory tract infections.

Objectives To evaluate the efficacy and safety of h um an hepatocyte transplantation in the treatment of severe hepatitis induced by dr ugs. Methods The primary human hepatocytes were isolated from t he liver of a healthy donor, and they were then cryopreserved. The thawed hepato cytes were transplanted into the patient's spleen via a femoral artery catheter. 2?10~10 hepatocytes were harvested, and 70% of thawed hepatocytes were vi able, and 2?10~9 vital hepatocytes were transplanted. Results One month after the transplantation, clinical symptoms of the recipient were a meliorated obviously, and the levels of BIL, NH3, ALT and AST lowered, while PA elevated. 50 days after discharge from the hospital it was found that biochemica l parameters returned to normal values, and the hepatocyte signal could be detec ted in the spleen with MRI. Conclusion Hepatocyte transplantati on is safe and efficacious.

Objective To identify the LongSAGE tags from yeast LongSAGE library and hyphal LongSAGE library of Candida albicans. Methods The technique called the generation of longer cDNA fragments from serial analysis of gene expression (SAGE) tags was used for gene identification (GLGI). The SAGE tags of 17 bases were converted into their corresponding 3′ cDNA fragments covering hundred bases, and the sequence was analyzed. Results Fifteen out of twenty tags were stably and efficiently extended with the base size between 200-1 000 bp. The sequence was considered to represent a known gene since it matched a full-length transcript sequence with over 95% similarity in the same orientation, including the same 17-bp SAGE tag sequence. Conclusion By using novel SAGE tags as primer, we can identify efficiently many novel transcripts. A combined application of SAGE-GLGI can be used to define the boundary of expressed genes in the genomic sequences in Candida albicans and in other eukaryotic genomes.

AIM: To establish the quality standard for Jixiangcao Buccal Tablets(Herba Reineckea Camea, Radix Asteris, Heaba Houttugnlae, Pericarpium Papaveris, Heaba Ephedrae etc.). METHODS: TLC was used for the identification of Herba Reineckea Camea and Pericarpium Papaveris. HPLC was used for the determination of ephedrine hydrocholoride in the Buccal tablets. RESULTS: TLC identification was highly specific and the spots were clear. The linear range for ephedrine hydrocholoride was in the range of 0.404～2.02?g and its average recovery was 97.03% and RSD 2.2%, respectively. CONCLUSION: The quality standard is able to effectively control the quality of Jixiangcao Buccal Tablets.

The inorganic drug of the metal complexes has been a new anticancer drug of chemical therapy,since cisplatin was used in clinic as an anticancer drug.This review introduced the mechanism of anticancer action of the anticancer drugs of platinum and other metallic complexes and their pharmacological effect,and the research progress in anticancer drugs of the metallic complexes were reviewed in this article.

Objective: To construct retroviral vector pLDAAOSN and observe the function of DAAO gene. Methods: With recombinant DNA technology, DAAO cDNA was cloned into retroviral vector (pLDAAOSN). The vector was transfected into ?XNA cells by CaPO4 method, and the DAAO cDNA was transferred by recombinant retroviral vector into leukemia cell line K562. The positive clones were obtained after G418 selection and termed K DAAO. PCR and in situ hybridization were used to identify the integration and expression of DAAO gene in K DAAO. In order to observe the function of DAAO, K DAAO was treated with 50 mm/L D-Ala. Results: Results of plasmid pLDAAOSN digested with KpnⅠ and the sequence determination confirmed pLDAAOSN contains full-length DAAO cDNA. Infectious titer generated by the packaging cells was 5.2?10 6 CFU/ml. PCR and in situ hybridization analysis showed integration of DAAO gene in K DAAO and expression of DAAO gene at mRNA level. Preliminary observation suggested that D-Ala could effectively kill K DAAO. Conclusion: Retroviral vector pLDAAOSN may be useful for futher study of gene therapy in cancer.

Objective To investigate the biological characteristics of mouse erythrolenkemia cell FBL-3.Methods The morphological feature, growth characters, clone formation and immunochemistry of mouse erythroleukemia cell FBL-3 were examined by light microscope. The cell cycle distribution and expression of MHC were detected by flow cytometry. Drug sensitivity was measured by MTT assay. Tumorigenicity was evaluated after intravenous injection FBL-3 cells into C57BL/6 mice. Results FBL-3 cells were smaller,fusiform or polygon, adherence. Doubling time was 24.12 h. The clone formation rates were (35.23±1.44) %and (60.27±5.56) % at 14 th and 21 th day, respectively. The reactions for PAS and chloracetic acid were positive, while the POX, NAP and butanoic acid reactions were negative. The cell cycle distribution was as follow: G0/G1 phase (50.9±2.5) %, S phase (36.3±1.4) %, G2/M phase (13.8±0.8) %. The IC50 of FBL-3 cells to Ara-C, VDS, DDP, MMC and MTX were (0.49±0.04), (0.87±0. 09), (3.77±0.32), (1.66±0.16) μmol/L and (2.77±0.24) nmol/L, respectively. Chromosome number was at 34 to 41. MHC of FBL-3 cell was H-2b. Sexual gene Sry was positive. All C57BL/6 mice were morbidity with erythroblastic leukemia when FBL-3 cells had been intravenously inoculated. There was a linear relationship between the survival time and the number cell injected. The main targets for the leukemic FBL-3 cells were liver, spleen, kindey and lung. Conclusion FBL-3 cell has typical features of mouse leukemia cell, was easily cultured in vitro and tumorigenesised in C57BL/6 mice. FBL-3 cell could be as a satisfactory tool for the research of leukemia.