MicroRNAs(miRNAs) are a class of micro,endogenously-initiated non-coding RNAs that negatively regulate gene expression at the post-transcriptional level. miRNAs have ability to negatively regulate the expression of genes involved in cell differentiation,proliferation,apoptosis and tumorigenesis.miRNAs could act as potential oncogenes and tumor repressors. Recent findings have demonstrated that miRNAs play critical roles in human gastric cancer.In this article,we reviewed the recent research progresses about the role of miRNAs in tumorigenesis,invasion and metastasis of gastric cancer and highlight the most significant and latest findings of original researches on potential implications of miRNA as a new class of biomarkers and therapeutic target in gastric cancer.

MicroRNA (miRNA) is a class of micro,endogenously-initiated non-coding RNA that negatively regulate gene expression at the post-transcriptional level and involved in tumorigenesis.Polymorphisms in miRNA sequences (miRSNP) alter mature miRNA levels and target mRNA selection.Recent findings have demonstrated that miRSNP mediated epigenetic alteration of miRNA can be one of the mechanism in tumor progression and may play critical role of hallmarks in cancer susceptibility.This review described the most recent advances in the field of hot miRSNP and susceptibility of tumour.

Objective To observed the changes of plasma levels of NT‐proBNP and hs‐CRP in patients with different types of coronary heart disease and investigate its clinical value .Methods 156 patients with CHD diagnosed by coronary computed tomo‐graphy angiography(CTA) were enrolled in this study ,including 35 cases of acute myocardial infarction(AMI) ,63 cases of unstable angina(UAP) and 58 cases of stable angina(SAP) .And 61 persons without CHD were selected as the control(CTR) .Plasma levels of NT‐proBNP and hs‐CRP were detected by the methods of immmunofluorescent antibody technic reader and turbidimetry ,respec‐tively .Results Plasma NT‐proBNP levels of AMI ,UAP ,SAP and CTR group were (1 903 .99 ± 2 055 .21) ,(897 .27 ± 947 .34) , (677 .98 ± 718 .12) ,(129 .39 ± 126 .49)ng/L ,respect ively .Plasma hs‐CRP levels were (28 .47 ± 20 .49) ,(12 .68 ± 8 .64) ,(10 .56 ± 7 .17) ,(2 .82 ± 1 .23)mg/L ,respectively .Plasma levels of NT‐proBNP and hs‐CRP in AMI group were obviously higher than those in UAP ,SAP and CTR group(P<0 .05) .Plasma levels of NT‐proBNP and hs‐CRP in UAP group were obviously higher than those in CTR group(P<0 .05) .There was no significant differences in these markers between SAP group and control group(P>0 .05) . Plasma BNP levels were positively correlated with plasma hs‐CRP .Conclusion Plasma levels of NT‐proBNP and hs‐CRP are asso‐ciated with different clinical phenotyes of CHD .Those markers may be helpful to the risk stratification and prognosis of CHD .

Objective To analyze the expression profile of specific miRNA of gastric cancer tissues.Methods miRNA were isolated from five paired gastric cancer tissues and corresponding paracancerous normal tissues.To identify the profile of gastric cancer,miRNA hybridization and cluster analysis were processed by significance analysis of microarrays (SAM,version 2.1) and cluster software.The relative expression of hsa-miR-30c,hsa-miR-196a and hsa-miR-193b were validated by fluorescence Real-Time PCR using taqman probe.Further hsa-miR-30c taget gene was predictived by online software.Results Revealed by miRNA microarray detection,there were 16 miRNA with differential expression in gastric cancer tissues and para-cancerous normal tissues,among which HS_100,hsa-miR-27a,hsa-miR-214*,solexa-555-1991 and hsa-miR-196 were significantly up-regulated,and hsa-miR-502-3p,hsa-miR-29c*,hsa-miR-64,hsa-miR-193b,hsa-miR-551b,hsa-miR-30c,hsa-miR-582-5p,hsa-miR-625*,hsa-miR-660,hsa-miR-363 and hsa-miR-486-5p were down-regulation.The relative expression of hsa-miR-30c,hsa-miR-196a and hsa-miR-193b was in high concordance with microarray.Conclusion There is specific miRNA expression profile in gastric cancer tissues,among which differentially-expressed miRNAs may be involved in the regulation of gastric cancer progress and serve as new potential biomarkers.

For the problems that 3 first-class ternary hospitals which are not directly affiliated to medical universities are facing in cultivating high-educated staff's scientific abilities,analyze the importance to carry out scientific work in clinic and discuss how to improve their scientific abilities from hospitals,departments and high-educated staff themselves.

The inorganic drug of the metal complexes has been a new anticancer drug of chemical therapy,since cisplatin was used in clinic as an anticancer drug.This review introduced the mechanism of anticancer action of the anticancer drugs of platinum and other metallic complexes and their pharmacological effect,and the research progress in anticancer drugs of the metallic complexes were reviewed in this article.

The Caco-2 cell model was widely applied in the research of absorption,metabolism and toxicity of drugs, especially in the aspect of anticancer,inorganic and traditional Chinese medicine,It has become an important tool of the study on medicine.

Objective: To investigate the different efficacy of Yinchenhao Decoction (YCHD), a compound traditional Chinese herbal medicine, for liver cirrhosis induced by dimethylnitrosamine (DMN) or carbon tetrachloride (CCl(4)) in rats. Methods: To induce liver fibrosis, 0.5% DMN solution (2mL/kg body weight, i.p.) was given three consecutive days a week to male Wistar rats for 4 weeks. Cirrhotic rats were randomly divided into DMN group, YCHD group, Xiaochaihu decoction group by the end of the fourth week to accomplish a 2-week recipe treatment course. In CCl(4)-induced liver fibrosis model, 50% CCl(4)-olive solution was injected subcutaneously to rats at a dose of 2 mL/kg body weight twice a week to duplicate rat cirrhosis model. After 8 weeks, rats were divided into CCl(4) group, CCl(4) plus YCHD group and Xiaochaihu decoction group. For the YCHD group, YCHD was administered intragastrically once a day for 4 weeks. For DMN or CCl(4) model, by the end of 6 or 12 weeks respectively, rats were sacrificed for sampling to detect liver function, hepatic histological changes, hydroxyproline (Hyp) content and apoptosis-related gene expressions. Results: In DMN liver fibrosis model, hepatic fibrosis was obvious at week 2 and cirrhosis was evident at week 4 in DMN-treated rats. Compared to 6-week DMN group, hepatic pathological changes and liver function were improved significantly and content of Hyp decreased remarkably in YCHD group. In CCl(4)-induced liver fibrosis model, hepatic fibrosis was obvious at 8 weeks and cirrhosis was evident at 12 weeks in CCl(4)-treated rats. Compared to 12-week CCl(4) group, hepatic pathological changes and liver function were not obviously improvement in YCHD group. The results of gene chip showed that YCHD significantly decreased Fas, Bax and caspase-3 gene expressions, and increased Bcl-xL gene expression in the liver of DMN model. However, in the model induced by CCl(4), YCHD did not inhibit hepatocyte apoptosis induced by CCl(4), but increased tyrosine kinase receptor gene expression by 4.8 times. Conclusion: YCHD exerts more significant therapeutic effects on DMN-induced than CCl(4)-induced cirrhosis in rats in Hyp content and pathological change in liver tissue.

This study was aimed to reveal the material basis on different diseases of the same syndrome damp-heat syndrome from the level of metabonomics.The typical damp-heat syndrome patients diagnosed as chronic viral hepatitis B,non-alcoholic fatty liver disease,or chronic glomerulonephritis were included,with 30 cases in each disease.There were 30 healthy volunteers in the control group.The serum samples were detected by UPLC-QTOFMS and GC-TOFMS.And then,the results were analyzed by variance analysis in order to find out the generality and specificity of metabolic material in three different diseases with damp-heat syndrome.The results showed that through comparisons of different diseases with damp-heat syndrome,as well as the healthy group as control,it was revealed that inosine,uridine,aspartic acid,oleic acid glyceride and lactate were the same substances of three diseases of damp-heat syndrome.It was concluded that based on metabonomics,as for three different diseases with damp-heat syndrome,there were different substances among different diseases,but common substances related to damp-heat syndrome.Thus,it provided objective evidences for the theory of different diseases of the same syndrome in traditional Chinese medicine (TCM) from the level of metabonomics.

Liver cirrhosis is the final outcome of various chronic liver diseases. In recent years, along with the rapid development of molecular biology and cell biological engineering technology, liver regeneration based on stem cell transplantation technique has become a new research hotspot for treatment of acute and chronic hepatic failure. Here we review basic and clinical studies on different types of stem cells for the treatment of liver cirrhosis and optimal choices of the stem cell type used for transplantation based on specificity of patients' particular status. A large number of experimental studies reveal that the technology of stem cell for the treatment of liver cirrhosis has gradually become mature with a broad prospect of application in the field of liver regeneration. This technology that holds enormous potential for treatment will bring hopes to the patients with end-stage liver cirrhosis.